作物学报 ›› 2015, Vol. 41 ›› Issue (07): 989-997.doi: 10.3724/SP.J.1006.2015.00989
• 作物遗传育种·种质资源·分子遗传学 • 下一篇
张天泉**,郭爽**,邢亚迪,杜丹,桑贤春,凌英华,何光华*
ZHANG Tian-Quan**,GUO Shuang**,XING Ya-Di,DU Dan,SANG Xian-Chun,LING Ying-Hua,HE Guang-Hua*
摘要:
叶色突变体是研究高等植物光合作用、叶绿素代谢途径、叶绿体结构与功能分子机理的理想材料。本研究从EMS (ethyl methane sulfonate)处理的缙恢10号(Oryza sativa L. ssp. indica)诱变群体中发现了一个苗期呈现黄绿色、抽穗期渐变为淡绿色的叶色突变体,命名为yellow green leaf 9 (ygl9)。与野生型相比,ygl9苗期和分蘖期光合色素极显著降低,抽穗期光合色素显著降低,气孔长度、气孔导度和蒸腾速率极显著增加,净光合速率无明显变化。透射电镜观察表明,ygl9的嗜锇小体增多、基粒模糊、基质片层减少且疏松,但叶绿体结构基本完整。遗传分析显示该突变性状受一对隐性核基因调控。利用西农1A/ygl9 F2群体中的759株隐性单株,最终将YGL9定位在第3染色体短臂SSR标记S03-1和InDel标记Ind03-19之间,遗传距离分别为0.13 cM和0.07 cM,物理距离为63 kb。本研究为YGL9基因的克隆和功能分析奠定了基础。
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