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作物学报 ›› 2016, Vol. 42 ›› Issue (02): 159-169.doi: 10.3724/SP.J.1006.2016.00159

• 作物遗传育种·种质资源·分子遗传学 •    下一篇

利用3个F2群体整合高密度栽培种花生遗传连锁图

郭建斌1,2,黄莉1,成良强1,陈伟刚1,任小平1,陈玉宁1,周小静1,沈金雄2,姜慧芳1,*   

  1. 1中国农业科学院油料作物研究所/农业部油料作物生物学与遗传育种重点实验室,湖北武汉 430062;2华中农业大学植物科学技术学院,湖北武汉 430070
  • 收稿日期:2015-06-24 修回日期:2015-11-20 出版日期:2016-02-12 网络出版日期:2015-12-07
  • 通讯作者: 姜慧芳, E-mail:peanut@oilcrops.cn, Tel: 027-86711550
  • 基金资助:

    本研究由国家自然科学基金项目(31271764, 31371662, 31471534, 31461143022),国家重点基础研究发展计划(973计划)项目(2011CB109300),农业部农作物种质资源保护项目(NB2010-2130135-28B)和国家现代农业产业技术体系建设专项(CARS-14-花生种质资源评价)。

An Integrated Genetic Linkage Map from Three F2 Populations of Cultivated Peanut (ArachishypogaeaL.)

GUO Jian-Bin1,2,HUANG Li1,CHENG Liang-Qiang1,CHEN Wei-Gang1,REN Xiao-Ping1,CHEN Yu-Ning1,ZHOU Xiao-Jing1,SHEN Jin-Xiong2,JIANGHui-Fang1,*   

  1. 1Oil Crops Research Institute of China Academy of Agricultural Sciences/Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062,China; 2College of Plant Science & Technology of Huazhong Agricultural University, Wuhan 430070,China
  • Received:2015-06-24 Revised:2015-11-20 Published:2016-02-12 Published online:2015-12-07
  • Supported by:

    This study was supported by the Natural Science Foundation of China(31271764, 31371662, 31471534, 31461143022),the National Key Basic Research Program of China(973 Program),the Crop Germplasm Resources Protection Project(NB2010-2130135-28B),and the NationalModern Agro-industry Technology System (CARS-14-peanut germplasm resource evaluation).

摘要:

遗传图谱的构建及整合是开展花生分子育种研究的基础,利用多个作图群体整合遗传图谱是解决图谱标记密度低的有效途径。本研究采用基于锚定

SSR标记的作图策略,构建3个F2群体3张遗传连锁图,利用JoinMap3.0软件整合图谱,获得一张包含20个连锁群、792个位点、总遗传距离为2079.5cM、标记间平均距离为2.63cM的整合图谱,各连锁群标记数在20~66个之间,遗传距离在59.1~175.8cM之间。将3个分离群体中检测到的与荚果及种子大小相关的QTL区段与整合连锁图的标记比较发现,各群体中检测到的位于各染色体上的QTL在整合图谱中都能出现,有些QTL标记区间在整合图谱中存在更多的标记,为今后利用这些标记进行精细定位奠定了基础。

关键词: 花生, SSR, 整合图谱

Abstract:

The genetic linkage map is important for peanut molecular breeding. Construction of integrating genetic linkage map using multiple populations is an effective approach to increase the marker density of map. Three maps were constructed with three F2 populations, respectively in the present study. Based on anchored SSR markers in the three maps, we constructed anew map with 792 SSR loci and total map distance of 2079.5cM. The length of linkage groups varied from59.1 to175.8cM, and the number ofmarkers wasfrom20 to 66 inthe integrated linkages groups.Comparingthe intervals of QTLslinked to the pod size and seed size in the three F2 populations withthe markers in the integrated linkage groups, all the QTLs linked to the pod size and seed size could be found in the integrated map. Some intervals of QTLs had more markers in the integrated map than in the F2 linkage groups in the present study. The markers in the intervals of QTLs of the integrated map could be used for fine mapping.

Key words: Cultivated peanut, SSR, Integrated genetic mapping

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