作物学报 ›› 2016, Vol. 42 ›› Issue (02): 170-179.doi: 10.3724/SP.J.1006.2016.00170
原换换1,2,**,孙广华1,2,**,闫蕾2,郭林2,樊小聪1,2,肖阳3,孟凡华2,宋梅芳2,4,詹克慧1,杨青华1,*,杨建平1,2,*
YUAN Huan-Huan1,2,**,SUN Guang-Hua1,2,**,YAN Lei 2,GUO Lin2,FAN Xiao-Cong1,2,XIAO Yang3,MENG Fan-Hua2,SONG Mei-Fang2,4,ZHAN Ke-Hui1,YANG Qing-hua1,*, YANG Jian-Ping1,2,*
摘要:
丝氨酸/苏氨酸蛋白磷酸酶6亚基(catalytic subunits of Ser/Thr protein phosphatase 6, PP6C)是PP6全酶的催化亚基。在模式植物拟南芥中的研究表明,PP6C参与生长素极性运输、脱落酸信号转导和光信号转导途径介导的开花调控。为了明确玉米丝氨酸/苏氨酸蛋白磷酸酶6亚基(ZmPP6C)的蛋白结构特征与同源蛋白间的进化关系,采用RT-PCR方法克隆了ZmPP6C的全长基因。序列分析表明,ZmPP6C开放阅读框为912个核苷酸,编码303个氨基酸残基,包含PP2A的催化亚基PP2Ac结构域;系统进化树分析表明,PP6C蛋白在进化上较为保守,并且与高粱的PP6C蛋白相似性更高。对玉米自交系B73的ZmPP6C基因进行器官特异性表达分析表明,其表达量在成株期叶片中最高,是根中的7.9倍;ZmPP6C能够响应不同光质处理,且受远红光和红光的影响较大;也能响应长日和短日处理,在长日条件下的光照和黑暗阶段各有一个明显的表达高峰,在短日条件下的光照和黑暗阶段分别有2个和3个表达峰值;同时,ZmPP6C还响应高渗透、盐渍和淹水等胁迫处理,出现明显的上调表达。结果表明,ZmPP6C在玉米光信号转导、开花诱导与胁迫应答中发挥重要作用,其分子与生化机制值得进一步探讨。
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