作物学报 ›› 2016, Vol. 42 ›› Issue (02): 170-179.doi: 10.3724/SP.J.1006.2016.00170
原换换1,2,**,孙广华1,2,**,闫蕾2,郭林2,樊小聪1,2,肖阳3,孟凡华2,宋梅芳2,4,詹克慧1,杨青华1,*,杨建平1,2,*
YUAN Huan-Huan1,2,**,SUN Guang-Hua1,2,**,YAN Lei 2,GUO Lin2,FAN Xiao-Cong1,2,XIAO Yang3,MENG Fan-Hua2,SONG Mei-Fang2,4,ZHAN Ke-Hui1,YANG Qing-hua1,*, YANG Jian-Ping1,2,*
摘要:
丝氨酸/苏氨酸蛋白磷酸酶6亚基(catalytic subunits of Ser/Thr protein phosphatase 6, PP6C)是PP6全酶的催化亚基。在模式植物拟南芥中的研究表明,PP6C参与生长素极性运输、脱落酸信号转导和光信号转导途径介导的开花调控。为了明确玉米丝氨酸/苏氨酸蛋白磷酸酶6亚基(ZmPP6C)的蛋白结构特征与同源蛋白间的进化关系,采用RT-PCR方法克隆了ZmPP6C的全长基因。序列分析表明,ZmPP6C开放阅读框为912个核苷酸,编码303个氨基酸残基,包含PP2A的催化亚基PP2Ac结构域;系统进化树分析表明,PP6C蛋白在进化上较为保守,并且与高粱的PP6C蛋白相似性更高。对玉米自交系B73的ZmPP6C基因进行器官特异性表达分析表明,其表达量在成株期叶片中最高,是根中的7.9倍;ZmPP6C能够响应不同光质处理,且受远红光和红光的影响较大;也能响应长日和短日处理,在长日条件下的光照和黑暗阶段各有一个明显的表达高峰,在短日条件下的光照和黑暗阶段分别有2个和3个表达峰值;同时,ZmPP6C还响应高渗透、盐渍和淹水等胁迫处理,出现明显的上调表达。结果表明,ZmPP6C在玉米光信号转导、开花诱导与胁迫应答中发挥重要作用,其分子与生化机制值得进一步探讨。
[1] Terol J, Bargues M, Carrasco P, Pérez-Alonso M, Paricio N. Molecular characterization and evolution of the protein phosphatase 2A B’ regulatory subunit family in plants. Plant Physiol, 2002, 129: 808–822[2] Moorhead G B, Trinkle-Mulcahy L, Ulke-Lemée A. Emerging roles of nuclear protein phosphatases. Nat Rev Mol Cell Biol, 2007, 8: 234–244[3] Cohen P T. Novel protein serine/threonine phosphatases: variety is the spice of life. Trends Biochem Sci, 1997, 22: 245–251[4] Dai M, Xue Q, Mccray T, Margavage K, Chen F, Lee J H, Nezames C D, Guo L, Terzaghi W, Wan J, Deng X W, Wang H. The PP6 phosphatase regulates ABI5 phosphorylation and abscisic acid signaling in Arabidopsis. Plant Cell, 2013, 25: 517–534[5] 刘钊, 贾霖, 贾盟, 关明俐, 曹英豪, 刘丽娟, 曹振伟, 李莉云, 刘国振. 水稻PP2Ac类磷酸酶蛋白质在盐胁迫下的表达. 中国农业科学, 2012, 45: 2339–2345Liu Z, Jia L, Jia M, Guang L M, Cao Y H, Liu L J, Cao Z W, Li L Y, Liu G Z. Expression on profiling of rice PP2Ac type phosphatase proteins in seedlings under salt stressed conditions. Sci Agric Sin, 2012, 45: 2339–2345 (in Chinese with English abstract)[6] Kim D H, Kang J G, Yang S S, Chung K S, Song P S, Park C M. A phytochrome-associated protein phosphatase 2A modulates light signals in flowering time control in Arabidopsis. Plant Cell, 2002, 14: 3043–3056[7] Farkas I, Dombrádi V, Miskei M, Szabados L, Koncz C. Arabidopsis PPP family of serine /threonine phosphatases. Trends Plant Sci, 2007, 12: 169–176[8] Gälweiler L, Guan C, Müller A, Wisman E, Mendgen K, Yephremov A, Palme K. Regulation of polar auxin transport by AtPIN1 in Arabidopsis vascular tissue. Science, 1998, 282: 2226–2230[9] Chen R, Hilson P, Sedbrook J, Rosen E, Caspar T, Masson P H. The Arabidopsis thaliana AGRAVITROPIC 1 gene encodes a component of the polar-auxin-transport efflux carrier. Proc Natl Acad Sci USA, 1998, 95: 15112–15117[10] Müller A, Guan C, Gälweiler L, Tänzler P, Huijser P, Marchant A, Parry G, Bennett M, Wisman E, Palme K. AtPIN2 defines a locus of Arabidopsis for root gravitropism control. EMBO J, 1998, 17: 6903–6911[11] Friml J, Benková E, Blilou I, Wisniewska J, Hamann T, Ljung K, Woody S, Sandberg G, Scheres B, Jürgens G, Palme K. AtPIN4 mediates sink-driven auxin gradients and root patterning in Arabidopsis. Cell, 2002, 108: 661–673[12] Petrásek J, Mravec J, Bouchard R, Blakeslee J J, Abas M, Seifertová D, Wisniewska J, Tadele Z, Kubes M, Covanová M, Dhonukshe P, Skupa P, Benková E, Perry L, Krecek P, Lee OR, Fink G R, Geisler M, Murphy A S, Luschnig C, Zazímalová E, Friml J. PIN proteins perform a rate-limiting function in cellular auxin efflux. Science, 2006, 312: 914–918[13] Dai M, Zhang C, Kania U, Chen F, Xue Q, McCray T, Li G, Qin G, Wakeley M, Terzaghi W, Wan J, Zhao Y, Xu J, Friml J, Deng X W, Wang H. A PP6-type phosphatase holoenzyme directly regulates PIN phosphorylation and auxin efflux in Arabidopsis. Plant Cell, 2012, 24: 2497–2514[14] Mauch-Mani B, Mauch F. The role of abscisic acid in plant-pathogen interactions. Curr Opin Plant Biol, 2005, 8: 409–414[15] Fujita Y, Fujita M, Satoh R, Maruyama K, Parvez M M, Seki M, Hiratsu K, Ohme-Takagi M, Shinozaki K, Yamaguchi-Shinozaki K. AREB1 is a transcription activator of novel ABRE-dependent ABA signaling that enhances drought stress tolerance in Arabidopsis. Plant Cell, 2005, 17: 3470–3488[16] Fujita Y, Fujita M, Shinozaki K, Yamaguchi-Shinozaki K. ABA-mediated transcriptional regulation in response to osmotic stress in plants. J Plant Res, 2011, 124: 509–525[17] Hauser F, Waadt R, Schroeder J I. Evolution of abscisic acid synthesis and signaling mechanisms. Curr Biol, 2011, 21: R346–355 [18] Hattori T, Totsuka M, Hobo T, Kagaya Y, Yamamoto-Toyoda A. Experimentally determined sequence requirement of ACGT-containing abscisic acid response element. Plant Cell Physiol, 2002, 43: 136–140[19] Busk P K, Pagès M. Regulation of abscisic acid induced transcription. Plant Mol Biol, 1998, 37: 425–435[20] Finkelstein R R, Lynch T J. The Arabidopsis abscisic acid response gene ABI5 encodes a basic leucine zipper transcription factor. Plant Cell, 2000, 12: 599–609[21] Lopez-Molina L, Chua N H. A null mutation in a bZIP factor confers ABA-insensitivity in Arabidopsis thaliana. Plant Cell Physiol, 2000, 41: 541–547[22] Liu X B, Zhang X Y, Wang Y X, Sui Y Y, Zhang S L, Herbert S J, Ding G. Soil degradation: a problem threatening the sustainable development of agriculture in Northeast China. Plant Soil Environ, 2010, 56: 87–97[23] Gao Y, Jiang W, Dai Y, Xiao N, Zhang C, Li H, Lu Y, Wu M, Tao X, Deng D, Chen J. A maize phytochrome interacting factor 3 improves drought and salt stress tolerance in rice. Plant Mol Biol, 2015, 87: 413–428[24] Xu Z S, Ni Z Y, Li Z Y, Li L C, Chen M, Gao D Y, Yu X D, Liu P, Ma Y Z. Isolation and functional characterization of HvDREB1—a gene encoding a dehydration-responsive element binding protein in Hordeum vulgare. J Plant Res, 2009, 122: 121–130[25] Tian S J, Mao X G, Zhang H Y, Chen S S, Zhai C C, Yang S M, Jing R L. Cloning and characterization of TaSnRK2.3, a novel SnRK2 gene in common wheat. J Exp Bot, 2013, 64: 2063–2080[26] Liu Z J, Yang X G, Hubbard K G, Lin X M. Maize potential yields and yield gaps in the changing climate of northeast China. Global Change Biol, 2012, 18: 3441–3454[27] Yang X, Lin E D, Ma S M, Ju H, Guo L P, Xiong W, Li Y, Xu Y L. Adaptation of agriculture to warming in Northeast China. Clim Change, 2007, 84: 45–58[28] Sun H, Tonks N K. The coordinated action of protein tyrosine phosphatases and kinases in cell signaling. Trends Biochem Sci, 1994, 19: 480–485[29] Hanada M, Ninomiya-Tsuji J, Komaki K, Ohnishi M, Katsura K, Kanamaru R, Matsumoto K, Tamura S. Regulation of the TAK1 signaling pathway by protein phosphatase 2C. J Biol Chem, 2001, 276: 5753–5759[30] 翁华, 冉亮, 魏群. 植物蛋白磷酸酶及其在植物抗逆中的作用. 植物学通报, 2003, 20: 609–615Weng H, Ran L, Wei Q. Protein phosphatases and their functions in plant response to environmental stress. Chin Bull Bot, 2003, 20: 609–615 (in Chinese with English abstract)[31] Rajeevan M S, Ranamukhaarachi D G, Vernon S D, Unger E R. Use of real-time quantitative PCR to validate the results of cDNA array and differential display PCR technologies. Methods, 2001, 25: 443–451[32] Shi Y. Serine/Threonine phosphatases: mechanism through structure. Cell, 2009, 139: 468–484[33] Fankhauser C, Chory J. Light control of plant development. Annu Rev Cell Dev Biol, 1997, 13: 203–229[34] Neff M M, Fankhauser C, Chory J. Light: An indicator of time and place. Genes Dev, 2000, 14: 257–271[35] Ma L, Li J, Qu L, Hager J, Chen Z, Zhao H, Deng X W. Light control of Arabidopsis development entails coordinated regulation of genome expression and cellular pathways. Plant Cell, 2001, 13, 2589–2607[36] 李潮海, 刘奎. 不同产量水平玉米杂交种生育后期光合效率比较分析. 作物学报, 2002, 28: 379–383 Li C H, Liu K. Analysis of photosynthesis efficiency of maize hybrids with different yield in the later growth stage. Acta Agron Sin, 2002, 28: 379–383 (in Chinese with English abstract)[37] Smith H. Phytochrome transgenics: functional, ecological and biotechnological applications. Semin Cell Biol. 1994, 5: 315–325[38] Bae G, Choi G. Decoding of light signals by plant phytochromes and their interacting proteins. Annu Rev Plant Biol, 2008, 59: 281–311[39] Li J, Li G, Wang H, Deng X W. Phytochrome signaling mechanisms. America: American Society of Plant Biologists,2011. pp1-26[40] Quail P H. Phytochrome photosensory signalling networks. Nat Rev Mol Cell Biol, 2002, 3: 85–93[41] 詹克慧, 李志勇, 侯佩, 习雨琳, 肖阳, 孟凡华, 杨建平. 利用修饰光敏色素信号途径进行品种改良的可行性. 中国农业科学, 2012, 45: 3249−3255Zhan K H, Li Z Y, Hou P, Xi Y L, Xiao Y, Meng F H, Yang J P. A new strategy for crop improvement through modification of phytochrome signaling pathways. Sci Agric Sin, 2012, 45: 3249−3255[42] Boylan M T, Quil P H. Oat phytochrome is biologically active in transgenic tomatoes. Plant Cell, 1989, 1: 765−773[43] Nagatani A, Kay S A, Deak M, Chua N H, Furuya M. Rice type I phytochrome regulates hypocotyl elongation in transgenic tobacco seedlings. Proc Natl Acad Sci USA, 1991, 88: 5207−5211[44] Garg A K, Sawers R J, Wang H, Kim J K, Walker J M, Brutnell T P, Parthasarathy M V, Vierstra R D, Wu R J. Light-regulated overexpression of an Arabidopsis phytochrome A gene in rice alters plant architecture and increases grain yield. Planta, 2006, 223: 627−636[45] Thiele A, Herold M, Lenk I, Quail P H, Gatz C. Heterologous expression of Arabidopsis phytochrome B in transgenic potato influences photosynthetic performance and tuber development. Plant Physiology, 1999, 120: 73−815[46] Putterill J, Robson F, Lee K, Simon R, Coupland G. The CONSTANS gene of Arabidopsis promotes flowering and encodes a protein showing similarities to zinc finger transcription factors. Cell, 1995, 80: 847–857[47] Suárez-López P, Wheatley K, Robson F, Onouchi H, Valverde F, Coupland G. CONSTANS mediates between the circadian clock and the control of flowering in Arabidopsis. Nature, 2001, 410: 1116–1120[48] Guo H, Yang H, Mockder T C, Lin C. Regulation of flowering time by Arabidopsis photoreceptors. Science, 1998, 279: 1360–1363[49] Pineiro R P, Coupland G. The control of flowering time and floral identity in Arabidopsis. Plant Physiol, 1998, 17: 1–8[50] Samach A, Onouchi H, Gold S E, Ditta G S, Schwarz-Sommer Z, Yanofsky M F, Coupland G. Distinct roles of CONSTANS target genes in reproductive development in Arabidopsis. Science, 2000, 288: 1613–1616[51] 王翠玲, 程芳芳, 孙朝晖, 库丽霞, 陈晓, 陈彦惠. 玉米光周期敏感性的遗转特性及相关基因的研究进展. 玉米科学, 2008, 16: 11–14Wang C L, Cheng F F, Sun Z H, Ku L X, Chen X, Chen Y H. Advances in genetic research and related genes of photoperiod sensitivity in maize. J Maize Sci, 2008, 16: 11–14 (in Chinese with English abstract)[52] 李思远, 陈晓, 王新涛, 陈彦惠. 玉米光周期敏感类Hd6基因的克隆和实时定量表达分析. 作物学报, 2008, 34: 713−717Li S Y, Chen X, Wang X T, Chen Y H. Clone and quantitative analysis by real-time RT-PCR of photoperiod sensitive gene Hd6-like in maize. Acta Agron Sin, 2008, 34: 713−717 (in Chinese with English abstract)[53] Yu R M, Zhou Y, Xu Z F, Chye M L, Kong R Y. Two genes encoding protein phosphatase 2A catalytic subunits are differentially expressed in rice. Plant Mol Biol, 2003, 51: 295–311 |
[1] | 肖颖妮, 于永涛, 谢利华, 祁喜涛, 李春艳, 文天祥, 李高科, 胡建广. 基于SNP标记揭示中国鲜食玉米品种的遗传多样性[J]. 作物学报, 2022, 48(6): 1301-1311. |
[2] | 崔连花, 詹为民, 杨陆浩, 王少瓷, 马文奇, 姜良良, 张艳培, 杨建平, 杨青华. 2个玉米ZmCOP1基因的克隆及其转录丰度对不同光质处理的响应[J]. 作物学报, 2022, 48(6): 1312-1324. |
[3] | 王丹, 周宝元, 马玮, 葛均筑, 丁在松, 李从锋, 赵明. 长江中游双季玉米种植模式周年气候资源分配与利用特征[J]. 作物学报, 2022, 48(6): 1437-1450. |
[4] | 杨欢, 周颖, 陈平, 杜青, 郑本川, 蒲甜, 温晶, 杨文钰, 雍太文. 玉米-豆科作物带状间套作对养分吸收利用及产量优势的影响[J]. 作物学报, 2022, 48(6): 1476-1487. |
[5] | 陈静, 任佰朝, 赵斌, 刘鹏, 张吉旺. 叶面喷施甜菜碱对不同播期夏玉米产量形成及抗氧化能力的调控[J]. 作物学报, 2022, 48(6): 1502-1515. |
[6] | 徐田军, 张勇, 赵久然, 王荣焕, 吕天放, 刘月娥, 蔡万涛, 刘宏伟, 陈传永, 王元东. 宜机收籽粒玉米品种冠层结构、光合及灌浆脱水特性[J]. 作物学报, 2022, 48(6): 1526-1536. |
[7] | 单露英, 李俊, 李亮, 张丽, 王颢潜, 高佳琪, 吴刚, 武玉花, 张秀杰. 转基因玉米NK603基体标准物质研制[J]. 作物学报, 2022, 48(5): 1059-1070. |
[8] | 许静, 高景阳, 李程成, 宋云霞, 董朝沛, 王昭, 李云梦, 栾一凡, 陈甲法, 周子键, 吴建宇. 过表达ZmCIPKHT基因增强植物耐热性[J]. 作物学报, 2022, 48(4): 851-859. |
[9] | 刘磊, 詹为民, 丁武思, 刘通, 崔连花, 姜良良, 张艳培, 杨建平. 玉米矮化突变体gad39的遗传分析与分子鉴定[J]. 作物学报, 2022, 48(4): 886-895. |
[10] | 闫宇婷, 宋秋来, 闫超, 刘爽, 张宇辉, 田静芬, 邓钰璇, 马春梅. 连作秸秆还田下玉米氮素积累与氮肥替代效应研究[J]. 作物学报, 2022, 48(4): 962-974. |
[11] | 徐宁坤, 李冰, 陈晓艳, 魏亚康, 刘子龙, 薛永康, 陈洪宇, 王桂凤. 一个新的玉米Bt2基因突变体的遗传分析和分子鉴定[J]. 作物学报, 2022, 48(3): 572-579. |
[12] | 宋仕勤, 杨清龙, 王丹, 吕艳杰, 徐文华, 魏雯雯, 刘小丹, 姚凡云, 曹玉军, 王永军, 王立春. 东北主推玉米品种种子形态及贮藏物质与萌发期耐冷性的关系[J]. 作物学报, 2022, 48(3): 726-738. |
[13] | 渠建洲, 冯文豪, 张兴华, 徐淑兔, 薛吉全. 基于全基因组关联分析解析玉米籽粒大小的遗传结构[J]. 作物学报, 2022, 48(2): 304-319. |
[14] | 张倩, 韩本高, 张博, 盛开, 李岚涛, 王宜伦. 控失尿素减施及不同配比对夏玉米产量及氮肥效率的影响[J]. 作物学报, 2022, 48(1): 180-192. |
[15] | 苏达, 颜晓军, 蔡远扬, 梁恬, 吴良泉, MUHAMMAD AtifMuneer, 叶德练. 磷肥对甜玉米籽粒植酸和锌有效性的影响[J]. 作物学报, 2022, 48(1): 203-214. |
|