作物学报 ›› 2016, Vol. 42 ›› Issue (02): 180-189.doi: 10.3724/SP.J.1006.2016.00180
孟亚雄1,2,孟祎林1,2,汪军成1,2,司二静1,2,张海娟1,2,任盼荣1,2,马小乐1,2,李葆春1,3, 杨轲1,2,王化俊1,2*
MENG Ya-Xiong1,2,MENG Yi-Lin1,2,WANG Jun-Cheng1,2,SI Er-Jing1,2,ZHANG Hai-Juan1,2,REN Pan-Rong1,2,MA Xiao-Le1,2,LI Bao-Chun1,3,YANG Ke1,2,WANG Hua-Jun1,2,*
摘要:
利用92个SSR标记对108份青稞亲本材料进行多态性扫描,分析其遗传多样性,旨在寻找与农艺性状相关联的分子标记,为青稞杂交组合的配制及分子标记辅助育种提供依据。挑选48个多态性标记进行群体遗传结构分析,在此基础上采用Tassel 2.1 GLM (general linear model)和MLM (mixed linear model)方法进行标记与农艺性状的关联分析。共检测出156个等位变异,每个位点2~6个等位变异。供试群体的Shannon指数为0.6727~1.1368,材料间遗传相似系数为0.2250~1.0000,平均0.7585。通过群体遗传结构分析将供试材料划分成4个亚群。以GLM分析,发现12个与株高、穗长、穗粒数和分蘖数相关联的标记,对表型变异的解释率分别为11.5%~17.6%、19.4%~45.4%、15.4%~22.1%和29.2%;以MLM分析,发现8个与株高、分蘖数和小穗数相关的标记,各标记对表型变异的解释率分别为31.7%~49.8%、28.1%~37.2%、22.7%~32.7%。关联标记分布在基因组全部6个连锁群上。
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