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作物学报 ›› 2017, Vol. 43 ›› Issue (11): 1643-1649.doi: 10.3724/SP.J.1006.2017.01643

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

利用BSR-Seq定位小麦品种郑麦103抗条锈病基因YrZM103

张怀志1,谢菁忠2,陈永兴2,刘旭3,王勇1,闫素红3,杨兆生3,赵虹4,王西成4,贾联合5,曹廷杰4,*,刘志勇2,*   

  1. 1中国农业大学农学院, 北京 100193; 2中国科学院遗传与发育生物学研究所, 北京 100101; 3中国农业科学院棉花研究所, 河南安阳 455000；4河南省农业科学院小麦研究所, 河南郑州450002; 5河北省唐县职业技术教育中心，河北唐县 072350
  • 收稿日期:2017-01-13 修回日期:2017-07-23 出版日期:2017-11-12 网络出版日期:2017-08-10
  • 通讯作者: 刘志勇, E-mail: zyliu@genetics.ac.cn; 曹廷杰, E-mail: caotingjie893@163.com
  • 基金资助:

    本研究由国家重点研发计划项目(2017YFD0101802)资助。

Mapping Stripe Rust Resistance Gene YrZM103 in Wheat Cultivar Zhengmai 103 by BSR-Seq

ZHANG Huai-Zhi1,XIE Jing-Zhong2,CHEN Yong-Xing2,LIU Xu3,WANG Yong1,WU Qiu-Hong2,Lu Ping2,ZHANG De-Yun1,LI Miao-Miao1,GUO Guang-Hao1,YAN Su-Hong3,YANG Zhao-Sheng3,ZHAO Hong4,WANG Xi-Cheng4,JIA Lianhe5,CAO Ting-Jie4,*,LIU Zhi-Yong2,*   

  1. 1 College of Agronomy & Biotechnology, China Agricultural University, Beijing 100193, China; 2 State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China; 3 Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, China; 4Wheat Research Institute, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China; 5Tangxian Vocational and Technical Educational Center, Tangxian, Hebei 072350, China
  • Received:2017-01-13 Revised:2017-07-23 Published:2017-11-12 Published online:2017-08-10
  • Contact: 刘志勇, E-mail: zyliu@genetics.ac.cn; 曹廷杰, E-mail: caotingjie893@163.com
  • Supported by:

    This study was supported by the National Key Research and Development Program of China (2017YFD0101802).

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摘要：

郑麦103是一个高抗条锈病的小麦新品种，为明确其携带的抗病基因，用郑麦103与感条锈病品种农大399杂交构建分离群体，用条锈菌CYR32、CYR33和CRY34 (V26)混合菌系进行田间接种和成株期抗性鉴定，对214个F2:3家系的条锈病抗性进行遗传分析，初步确定郑麦103的抗条锈性由单个主效基因控制，定名为YrZM103。通过BSR-Seq技术开发了6个与YrZM103紧密连锁的分子标记，将YrZM103定位于染色体臂7BL分子标记ZM215和ZM221之间，遗传距离分别为11.8 cM和6.9 cM。利用7BL染色体上与其他已知抗条锈病基因紧密连锁的分子标记进行比较作图，发现YrZM103是不同于7BL末端其他抗条锈病基因的新基因。

关键词: 郑麦103, 条锈病, 分子标记, BSR-Seq, 小麦

Abstract:

Zhengmai 103 is a new wheat cultivar highly resistant to stripe rust. In order to detect the stripe rust resistance gene in Zhengmai 103, a segregating population was developed by making cross between Zhengmai 103 and a highly stripe rust susceptible wheat cultivar Nongda 399. The 214 F2 derived F2:3 progenies were inoculated with mixed prevailing Pst races CYR32, CYR33 and CYR34 (V26) in field condition for disease resistance evaluation at adult plant stage. Genetic analysis revealed that the stripe rust resistance of Zhengmai 103 is controlled by a single gene, temporarily designated YrZM103. By applying RNA-seq with bulked segregant analysis (BSA), six polymorphic markers were developed to map YrZM103 on chromosome 7BL flanked by markers ZM215 and ZM221 with genetic distances of 6.9 and 11.8 cM, respectively. Comparative genetic mapping indicated YrZM103 was located on different genetic interval from that of known stripe rust resistance genes on 7BL.

Key words: Zhengmai 103, Stripe rust, Molecular marker, BSR-Seq, Wheat

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