作物学报 ›› 2018, Vol. 44 ›› Issue (01): 144-156.doi: 10.3724/SP.J.1006.2018.00144
• 研究简报 • 上一篇
刘天鹏1,董孔军1,董喜存2,何继红1,刘敏轩3,任瑞玉1,张磊1,杨天育1,4
LIU Tian-Peng1,DONG Kong-Jun1,DONG Xi-Cun2,HE Ji-Hong1,LIU Min-Xuan3,REN Rui-Yu1,ZHANG Lei1,YANG Tian-Yu1,4,
摘要:
为构建物理诱变糜子突变体库,采用50、100、150、200和250 Gy剂量碳离子束(12C6+)辐照陇糜7号和晋黍9号种子,结合混合系谱法和系统聚类分别构建2个含52和79个株系的M5寡表型突变群体。田间试验结果显示,诱变M1出苗率随剂量增大明显降低,陇糜7号M1半致死剂量为150 Gy,晋黍9号M1半致死剂量为100 Gy,且100、150 Gy诱变下M4变异最多。不同诱变剂量下M5、M6表型稳定且株高、产量性状、成株色及粒色均表现出明显遗传差异。从2个M6群体中分别选择9和11个代表性株系,利用多态性SSR引物进行分子验证,与亲本相比,6对SSR引物在陇糜7号的9个株系中位点变异基因型数为1~2,在晋黍9号的11个株系中位点变异基因型数为1~4,突变群体存在丰富的遗传多样性。
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