欢迎访问作物学报，今天是
作物学报 ›› 2023, Vol. 49 ›› Issue (8): 2088-2096.doi: 10.3724/SP.J.1006.2023.23059
王娟(
), 徐相波, 张茂林, 刘铁山, 徐倩, 董瑞, 刘春晓, 关海英, 刘强, 汪黎明(), 何春梅()
WANG Juan(), XU Xiang-Bo, ZHANG Mao-Lin, LIU Tie-Shan, XU Qian, DONG Rui, LIU Chun-Xiao, GUAN Hai-Ying, LIU Qiang, WANG Li-Ming(), HE Chun-Mei()
摘要：
籽粒发育是决定玉米产量和品质的关键因素, 但目前对其遗传调控机制的研究还相当不完善。我们在田间筛选到一个玉米籽粒发育突变体, 表现为籽粒灌浆不充分, 胚和胚乳变小, 成熟籽粒干瘪或“空果皮”。该突变表型受单个隐性核基因控制。通过图位克隆的方式将候选突变基因定位到2号染色体1.1 Mb的区间内, 进一步研究发现, 该区间内Miniature1 (Mn1)基因在第1个外显子处发生hAT转座子插入, 导致Mn1基因表达下调和无义突变。此插入突变与突变体籽粒灌浆缺陷表型完全连锁, 该突变体被命名为mn1-m2。通过与mn1-89突变体进行等位测验, 确认mn1-m2即为Mn1基因的等位突变体。因此, 本研究鉴定了一个新的mn1等位突变体, 其突变位点及方式与已知mn1突变体均不相同, 完善了玉米籽粒突变体的种质资源信息, 也为Mn1调控籽粒发育机制的解析提供新的遗传材料。
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