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作物学报 ›› 2018, Vol. 44 ›› Issue (05): 716-722.doi: 10.3724/SP.J.1006.2018.00716

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

水稻咪草烟抗性的遗传分析及其紧密连锁分子标记的筛选与应用

费云燕1, 杨杰1,2,*(), 范方军1,2, 王芳权1,2, 李文奇1,2, 王军1,2, 朱金燕1,2, 仲维功1,2   

  1. 1江苏省农业科学院粮食作物研究所, 江苏南京 210014
    2扬州大学 / 江苏省粮食作物现代产业技术协同创新中心, 江苏扬州 225009
  • 收稿日期:2017-07-06 接受日期:2018-01-08 出版日期:2018-05-20 网络出版日期:2018-01-23
  • 通讯作者: 杨杰
  • 作者简介:

    第一作者联系方式: E-mail: suiyiyixinyisi@163.com

  • 基金资助:
    本项目由国家重点研发计划项目(2017YFD0100403), 江苏省现代农业重点研发项目(BE2015355), 江苏省农业科学院探索性项目(ZX(17)2014)和江苏省自然科学基金项目(BK20171326)资助

Genetic Analysis of Imazethapyr Resistance in Rice and the Closely Linked Marker Selection and Application

Yun-Yan FEI1, Jie YANG1,2,*(), Fang-Jun FAN1,2, Fang-Quan WANG1,2, Wen-Qi LI1,2, Jun WANG1,2, Jin-Yan ZHU1,2, Wei-Gong ZHONG1,2   

  1. 1 Institute of Food Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, Jiangsu, China
    2 Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops / Yangzhou University, Yangzhou 225009, Jiangsu, China
  • Received:2017-07-06 Accepted:2018-01-08 Published:2018-05-20 Published online:2018-01-23
  • Contact: Jie YANG
  • Supported by:
    This study was supported by the National Key Research and Development Program (2017YFD0100403), the Jiangsu Province Key Research and Development Program (Modern Agriculture) (BE2015355), the Exploratory Project of the Jiangsu Academy of Agricultural Sciences (ZX(17)2014), and the Project of Jiangsu Province Natural Science Foundation (BK20171326).

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摘要：

抗除草剂水稻的培育及推广能够提高除草效率, 取得巨大经济效益。本研究中金粳818为抗咪草烟资源, 以其与常规粳稻苏垦118杂交产生的F2群体对抗性基因进行遗传分析与分子定位表明, 其抗性表型受1对显性核基因控制, 位于水稻第2染色体SSR分子标记RM7413和RM7426之间。对该区间候选基因预测和测序发现, 咪草烟靶基因-乙酰乳酸合酶基因(ALS)在重要功能位点发生1个碱基的突变(G变为A), 导致一个氨基酸由丝氨酸(S)突变为天冬酰胺(N), 初步确定ALS是抗性表型的重要候选基因。标记RM7413、RM7426与ALS的物理距离分别为165 kb、1612 kb。以金粳818与南粳9108为亲本, 检测标记RM7413在辅助育种实践中的应用潜力, 对杂交种及其自交后代进行连续表型及标记选择, F7群体能够稳定遗传抗咪草烟性状, 表明RM7413在粳稻抗咪草烟辅助育种中具有巨大应用潜力。本研究结果为粳稻抗除草剂分子标记辅助改良奠定了基础。

关键词: 粳稻, 分子标记辅助育种, RM7413, 金粳818, 咪草烟

Abstract:

Cultivation and extension of herbicide-resistant rice can increase the efficiency of weed control and obtain great economic benefits. Here, Jinjing 818 was identified as an imazethapyr-resistant line, F2 population from the cross between Jinjing 818 and the conventional japonica rice Suken 118 was used for genetic analysis and gene mapping of the imazethapyr resistance in rice. The imazethapyr resistance in Jinjing 818 was controlled by a single dominant nuclear gene located between SSR markers RM7413 and RM7426 on the chromosome 2. Through gene-predicting and sequencing in this region, we found that the target gene of imazethapyr, acetolactate synthase gene (ALS), had a nucleotide mutation (G mutated into A) in its coding region, leading to the mutation of serine into asparagine. It could be preliminarily deduced that ALS is the candidate gene for imazethapyr resistance. The physical distances between RM7413, RM7426 and ALS were 165 kb, 1612 kb, respectively. Jinjing 818 and Nanjing 9108 were used as parents to detect the application potential of RM7413 in molecular marker assisted selection breeding (MAS). On the basis of selecting phenotype and RM7413 in the hybrid and its selfing generation, F7 population showed stable imazethapyr resistance, indicating that the great application potential of RM7413 in MAS and breeding for imazethapyr resistant rice. Our results will lay a foundation for breeding herbicide-resistant japonica rice by MAS.

Key words: Oryza sativa subsp. Keng, Molecular marker assisted selection, RM7413, Jinjing 818, Imazethapyr

表1

长江中下游主要推广的91份粳稻品种"

生育特性 Maturing type 品种 Cultivar
中熟中粳
Medium-maturing medium japonica rice		 连粳4号, 连粳7号, 连粳9号, 宁粳4号, 徐稻3号, 镇稻88, 镇稻99, 连粳10号, 连粳11, 华粳5号, 华粳6号, 淮稻8号, 淮稻11, 淮优粳2号, 盐稻11, 盐粳11, 武运粳21, 武运粳27, 华瑞稻1号, 淮糯11, 淮粳096, 扬中稻1号, 扬辐粳1号, 扬粳4308, 泗稻12
Lianjing 4, Lianjing 7, Lianjing 9, Ningjing 4, Xudao 3, Zhendao 88, Zhendao 99, Lianjing 10, Lianjing 11, Huajing 5, Huajing 6, Huaidao 8, Huaidao 11, Huaiyoujing 2, Yandao 11, Yanjing 11, Wuyunjing 21, Wuyunjing 27, Huaruidao 1, Huainuo 11, Huaijing 096, Yangzhongjing 1, Yangfujing 1, Yangjing 4308, Sidao 12
迟熟中粳
Late-maturing medium japonica rice		 淮稻5号, 盐稻8号, 盐稻9号, 南粳49, 南粳40, 南粳41, 南粳45, 扬育粳2号, 扬辐粳8号, 苏沪香粳, 武陵粳1号, 武育粳3号, 武运粳24, 华粳3号, 华粳4号, 华粳7号, 淮稻7号, 淮稻9号, 淮稻10号, 淮稻13, 盐粳9号, 盐粳10号, 宁粳5号, 武运粳4号, 武运粳11
Huaidao 5, Yandao 8, Yandao 9, Nanjing 49, Nanjing 40, Nanjing 41, Nanjing 45, Yangyujing 2, Yangfujing 8, Suluxiangjing, Wulingjing 1, Wuyunjing 3, Wuyunjing 24, Huajing 3, Huajing 4, Huajing 7, Huaidao 7, Huaidao 9, Huaidao 10, Huaidao 13, Yanjing 9, Yanjing 10, Ningjing 5, Wuyunjing 4, Wuyunjing 11
早熟晚粳
Early-maturing late
japonica rice		 宁粳1号, 南粳5055, 南粳44, 武香粳14, 南粳47, 武运粳7号, 镇稻18, 淮香稻15, 宁粳3号, 南粳42, 通粳981, 武粳13, 武粳15, 武香粳9号, 武运粳19, 武运粳23, 武运粳29, 常农粳4号, 常农粳5号, 常农粳7号, 镇稻7号, 镇稻10号, 镇稻15, 镇稻16, 镇糯19, 宁粳2号, 常粳144, 苏粳5号, 镇稻1号, 镇稻9424
Ningjing 1, Nanjing 5055, Nanjing 44, Wuxiangjing 14, Nanjing 47, Wuyunjing 7, Zhendao 18, Huaixiangdao 15, Ningjing 3, Nanjing 42, Tongjing 981, Wujing 13, Wujing 15, Wuxiangjing 9, Wuyunjing 19, Wuyunjing 23, Wuyunjing 29, Changnongjing 4, Changnongjing 5, Changnongjing 7, Zhendao 7, Zhendao 10, Zhendao 15, Zhendao 16, Zhennuo 19, Ningjing 2, Changjing 144, Sujing 5, Zhendao 1, Zhendao 9424
中熟晚粳
Medium-maturing late japonica rice		 南粳46, 扬粳4227, 武育粳18, 常农粳3号, 常农粳6号, 苏粳8号, 苏香粳2号, 镇稻12, 镇稻13, 镇稻17, 苏粳9号
Nanjing 46, Yangjing 4227, Wuyujing 18, Changnongjing 3, Changnongjing 6, Sujing 8, Suxiangjing 2, Zhendao 12, Zhendao 13, Zhendao 17, Sujing 9

图1

抗咪草烟水稻和咪草烟敏感水稻的表型 R: 抗咪草烟水稻金粳818; S: 感咪草烟水稻苏垦118。"

图2

亲本金粳818、苏垦118和F2群体中咪草烟抗性及感性苗表型 R: 抗咪草烟幼苗; S: 感咪草烟幼苗。"

图3

SSR标记RM7326、RM7413、RM7426、RM5221在咪草烟敏感植株中的基因型(部分图) 1: 抗性对照金粳818的基因型; 2: 敏感对照苏垦118的基因型; 其余为咪草烟敏感植株中基因型。"

图4

抗咪草烟基因的标记连锁图"

图5

SSR标记RM7413在F7的随机10个个体中的基因型1: 抗性对照金粳818的基因型; 2: 敏感对照南粳9108的基因型; M: DNA marker; 3~12: 10个随机个体的基因型。"

图6

SSR标记RM7413在常规粳稻中的基因型(部分图) 1: 抗性对照金粳818的基因型; 2: 敏感对照苏垦118的基因型; M: DNA marker; 其余为常规粳稻的基因型。"

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