欢迎访问作物学报，今天是
作物学报

作物学报 ›› 2018, Vol. 44 ›› Issue (8): 1169-1184.doi: 10.3724/SP.J.1006.2018.01169

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

甘蓝型油菜种子萌发期耐苯磺隆种质筛选与综合评价

王倩(),崔翠(),叶桑,崔明圣,赵愉风,林呐,唐章林,李加纳,周清元()   

  1. 西南大学农学与生物科技学院, 重庆400715
  • 收稿日期:2017-11-20 接受日期:2018-03-26 出版日期:2018-08-10 网络出版日期:2018-04-18
  • 通讯作者: 王倩,崔翠,周清元
  • 基金资助:
    国家现代农业产业技术体系建设专项(CARS-12);国家科技支撑计划项目(2013BAD01B03-12);重庆市社会事业与民生保障科技创新项目(cstc2016shmszx0756);西南大学博士启动基金项目(swu113064)

Screening and Comprehensive Evaluation of Germplasm Resources with Tribenuron-methyl Tolerance at Germination Stage in Rapeseed (Brassica napus L.)

Qian WANG(),Cui CUI(),Sang YE,Ming-Sheng CUI,Yu-Feng ZHAO,Na LIN,Zhang-Lin TANG,Jia-Na LI,Qing-Yuan ZHOU()   

  1. College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China
  • Received:2017-11-20 Accepted:2018-03-26 Published:2018-08-10 Published online:2018-04-18
  • Contact: Qian WANG,Cui CUI,Qing-Yuan ZHOU
  • Supported by:
    the China Agriculture Research System(CARS-12);the National Key Technology Support Program of China(2013BAD01B03-12);the Science and Technology Committee of Chongqing(cstc2016shmszx0756);the Doctoral Start-up Fund of Southwestern University(swu113064)

RichHTML

16

PDF

1149

PDF

24

被引次数

1

摘要：

本研究旨在探讨不同基因型甘蓝型油菜种子萌发期耐除草剂苯磺隆特性, 建立其筛选的评价体系, 选育萌发期耐苯磺隆的甘蓝型油菜品种(系)。采用苯磺隆耐性度量值(T值)、平均隶属函数值(ASF值)、加权耐性系数(WTC值)等指标及相关分析、频数分析、主成分分析、灰色关联度分析、聚类分析和逐步回归分析相结合的方法, 鉴定萌发期耐性。通过11份供试材料相对根长的方差分析, 确定了油菜萌发期耐苯磺隆种质资源筛选及评价研究的最佳苯磺隆浓度为25 mg kg -1。在此浓度胁迫下, 241份甘蓝型油菜的根长、芽长、率、发芽势、鲜重5个指标均存在显著性差异。根据T值的聚类分析, 将供试种质划分为5个苯磺隆耐性级别, 其中I级3份、II级30份、III级198份、IV级6份、V级4份。对苯磺隆耐性较强的甘蓝型油菜品种(系)为希望106、SWU95和WH-33, 可作为甘蓝型油菜苯磺隆耐性育种和耐性机制研究的重要资源。根长、鲜重、发芽率可作为评价油菜种质资源萌发期耐性的指标性状。

关键词: 甘蓝型油菜, 耐苯磺隆, 种质资源, 综合评价, 萌发期

Abstract:

The purpose of this study is to explore the characteristics of tribenuron-methyl tolerance in different genotypes of Brassica napus, and establish the evaluation system for screening and breeding new cultivars with tribenuron-methyl tolerance. The germination vigor was measured on the third day after treatment and the germination rate, shoot length, root length, dry weight, as well as fresh weight were measured on the seventh day. The identification of tolerance at germination stage was performed by tribenuron-methyl tolerance comprehensive evaluation value (T), average subordinative function value (ASF) and weight tribenuron-methyl tolerance coefficient (WTC) in correlation analysis, frequency analysis, principal component analysis, grey analysis, cluster analysis and stepwise regression analysis. Based on the variance analysis of relative root length index in 11 tested materials, we determined the optimum treatment concentration (25 mg kg -1) for selecting and evaluating the tolerant germplasm resources to tribenuron-methyl in napeseed. Under the optimum concentration, the single factor variance analysis showed significant differences in root length, shoot length, germination rate, germination vigor and fresh weight among 241 accessions of Brassica napus. The clustering analysis according to T value exhibited that the tested cultivars were roughly divided into five tribenuron-methyl tolerance grades, including three in grade I, 30 in grade II, 198 in grade III, six in grade IV, and four in grade V. We conclude that the varieties (lines) with strong tribenuron-methyl tolerance at germination stage are Xiwang 106, SWU95, and WH-33, which could be used as the germplasm materials for the study on tolerance breeding and mechanism. Furthermore, the root length, fresh weight and germination rate could be used as index traits to evaluate the tolerance of rapeseed germplasm resources during germination stage.

Key words: Brassica napus L, Tribenuron-methyl tolerance, germplasm resources, comprehensive evaluation, germination stage

表1

不同浓度下11份品种根长相对值的显著性分析"

品种(系)
Variety (line)		 苯磺隆浓度 Concentration of tribenuron-methyl
0.25 mg kg-1 2.50 mg kg-1 25.00 mg kg-1 250.00 mg kg-1
炎81-2 Yan 81-2 70.3±0.007 c 17.8±0.015 de 10.4±0.003 f 10.3±0.003 de
SWU92 82.5±0.030 b 19.3±0.009 de 14.5±0.002 def 11.7±0.011 de
SWU101 64.5±0.077 cd 15.3±0.017 de 12.5±0.001 ef 9.3±0.012 e
782 48.5±0.003 e 18.5±0.004 de 13.5±0.006 ef 12.9±0.002 cde
509 50.0±0.008 e 19.8±0.021 de 15.4±0.004 cdef 14.5±0.009 bcde
P18 106.3±0.091 a 36.0±0.031 a 31.1±0.002 a 28.8±0.016 a
甲预31棚 Jiayu 31 peng 24.6±0.010 f 22.4±0.013 cd 19.6±0.029 bcd 15.8±0.006 bcde
苏油1号 Suyou 1 95.7±0.039 a 26.1±0.008 bc 23.8±0.012 b 20.0±0.004 bc
至尊 Zhizun 85.2±0.002 b 29.3±0.030 ab 22.3±0.001 bc 21.4±0.019 ab
B265 96.4±0.044 a 31.1±0.054 ab 18.5±0.011 bcde 18.0±0.006 bcd
中双12 Zhongshuang 12 60.1±0.010 d 13.3±0.017 e 13.2±0.038 ef 13.5±0.059 cde

表2

苯磺隆胁迫下油菜萌发期各指标的变化"

项目
Item		 参数
Parameter		 根长
RL (cm)		 芽长
SL (cm)		 发芽势
GV (%)		 发芽率
GR (%)		 鲜重
FW (g)		 干重
DW (g)
对照
Control		 最大值 Max. 13.13 5.81 100.00 100.00 0.79 0.08
最小值 Min. 0.55 0.76 10.00 15.00 0.08 0.01
均值 Average 7.38** 2.27** 81.31** 87.94** 0.38** 0.04**
标准差 SE 1.61 1.24 19.87 14.47 0.11 0.01
变异系数 CV (%) 21.77 54.56 24.44 16.45 28.91 26.74
胁迫
Stress		 最大值 Max. 2.97 6.24 100.00 100.00 0.79 0.06
最小值 Min. 0.34 0.71 5.00 15.00 0.12 0.01
均值 Average 0.94** 2.37** 75.77** 83.22** 0.36** 0.04**
标准差 SE 0.38 1.30 21.47 16.66 0.10 0.01
变异系数 CV (%) 40.00 54.83 28.34 20.01 28.00 25.91
较对照变化 均值 Average -6.44 0.10 -5.54 -4.72 -0.02 0
Comparison with the control 变异系数 CV (%) 18.23 0.27 3.90 3.56 -0.91 -0.83

图1

241份甘蓝型油菜不同指标耐性系数(TC)的频次分布 RL: 根长; FW: 鲜重; GV: 发芽势; GR: 发芽率; SL: 芽长; DW: 干重。"

表3

供试油菜种质各指标的苯磺隆耐性系数"

参数
Parameter		 根长
RL		 芽长 发芽势 发芽率 鲜重 干重
SL GV GR FW DW
最大值 Max. 0.588 2.005 1.000 1.000 2.357 1.946
最小值 Min. 0.050 0.494 0.143 0.333 0.411 0.333
均值 Average 0.136** 1.052** 0.926** 0.942** 0.980** 1.055
标准差 SE 0.066 0.187 0.123 0.097 0.202 0.136
变异系数 CV (%) 48.950 17.800 13.269 10.276 20.593 12.856

表4

供试油菜种质各指标耐性系数的相关性"

指标
Index		 根长
RL		 芽长
SL		 鲜重
FW		 发芽率
GR		 发芽势
GV		 干重
DW
RL 1
SL 0.046 1
FW 0.042 0.215** 1
GR -0.150* -0.059 -0.018 1
GV -0.171** 0.031 0.022 0.687** 1
DW -0.128* 0.031 0.062 0.133* 0.097 1

表5

各综合指标的特征值、贡献率和主成分特征向量值"

主成分
Principal
component		 特征值
Eigen
value		 贡献率
Variance
contribution (%)		 累计贡献率
Accumulated variance
contribution (%)		 所测指标的特征向量值 Eigen vector of measured indicators
发芽率
GR		 发芽势GV 鲜重
FW		 根长
RL		 芽长
SL		 干重
DW
1 1.810 30.167 30.167 0.978 0.945 0.279 0.011 0.261 0.617
2 1.310 21.825 51.992 -0.358 -0.263 0.841 0.396 0.769 0.258
3 1.096 18.267 70.259 0.115 0.311 -0.263 0.904 0.195 -0.637
4 0.790 13.159 83.418 -0.044 -0.057 0.316 0.508 -0.755 0.323

表6

苯磺隆胁迫下油菜品种资源的ASF值、WTC值、T值和综合排序"

编号
Code		 ASF值 ASF value WTC值 WTC value T值 T value 综合排序
Final rank
数值Value 排序Order 数值Value 排序Order 数值Value 排序Order
1 0.55 35 0.55 30 0.28 73 40
2 0.46 167 0.53 67 -0.30 178 141
3 0.49 127 0.55 32 0.07 108 81
4 0.48 138 0.54 62 -0.16 154 124
5 0.44 192 0.50 164 -0.55 201 192
6 0.57 24 0.55 26 0.65 31 25
7 0.48 141 0.48 194 -0.29 177 175
8 0.42 213 0.46 208 -0.53 199 212
9 0.47 148 0.52 101 -0.29 176 148
10 0.54 49 0.55 33 0.55 35 35
11 0.47 147 0.52 114 -0.14 145 138
12 0.52 78 0.52 121 0.05 111 103
13 0.45 177 0.52 106 -0.62 207 165
14 0.51 84 0.53 84 0.23 80 74
15 0.42 206 0.52 100 -0.66 215 178
16 0.47 150 0.49 174 -0.01 121 153
17 0.47 154 0.50 145 -0.15 151 154
18 0.50 98 0.53 83 0.10 105 85
19 0.42 203 0.49 178 -0.72 218 209
20 0.50 110 0.52 99 0.13 98 100
21 0.45 184 0.50 144 -0.55 203 181
22 0.44 194 0.51 132 -0.52 198 179
23 0.44 196 0.48 183 -0.54 200 202
24 0.49 122 0.53 65 -0.14 146 116
25 0.46 164 0.50 163 -0.12 141 161
26 0.47 155 0.51 123 -0.30 179 158
27 0.40 218 0.46 212 -0.83 227 218
28 0.49 134 0.52 94 0.05 110 118
29 0.60 11 0.57 7 0.79 24 11
30 0.42 209 0.48 191 -0.60 206 210
31 0.52 68 0.54 57 0.02 118 70
32 0.53 58 0.52 98 0.22 81 67
33 0.59 14 0.56 13 0.96 15 12
34 0.48 143 0.52 104 -0.21 163 140
35 0.33 235 0.41 236 -1.28 234 235
36 0.16 241 0.36 241 -2.57 241 241
37 0.50 92 0.50 141 -0.01 119 123
38 0.25 238 0.38 238 -1.77 238 238
39 0.48 140 0.53 66 -0.10 138 122
40 0.47 157 0.54 49 -0.07 133 119
41 0.46 169 0.53 82 -0.43 191 152
42 0.50 107 0.53 63 0.03 116 86
43 0.41 215 0.49 166 -0.57 204 206
编号
Code		 ASF值 ASF value WTC值 WTC value T值 T value 综合排序
Final rank
数值Value 排序Order 数值Value 排序Order 数值Value 排序Order
44 0.46 162 0.50 147 -0.13 143 156
45 0.44 197 0.49 175 -0.39 186 194
46 0.49 120 0.54 47 -0.02 122 87
47 0.39 223 0.45 223 -0.74 219 223
48 0.51 89 0.54 40 0.35 64 54
49 0.54 43 0.53 81 0.35 63 52
50 0.53 52 0.55 25 0.21 82 46
51 0.42 204 0.47 206 -0.36 185 208
52 0.64 5 0.57 9 1.13 6 5
53 0.50 106 0.50 156 -0.07 135 134
54 0.50 108 0.51 137 0.36 60 99
55 0.44 187 0.48 186 -0.31 181 191
56 0.46 163 0.49 167 -0.17 157 164
57 0.45 182 0.47 197 -0.24 167 189
58 0.50 103 0.54 58 -0.15 152 106
59 0.46 168 0.48 185 -0.31 180 183
60 0.54 50 0.55 27 0.46 46 37
61 0.47 160 0.53 75 -0.33 182 144
62 0.51 83 0.53 86 0.33 67 66
63 0.32 236 0.42 235 -1.41 236 236
64 0.53 60 0.54 55 0.20 86 58
65 0.54 46 0.54 52 0.53 39 39
66 0.55 34 0.56 14 0.53 38 26
67 0.49 124 0.53 89 -0.27 172 131
68 0.50 104 0.53 73 -0.11 140 107
69 0.45 175 0.52 105 -0.39 188 162
70 0.52 71 0.52 102 0.18 88 78
71 0.39 222 0.44 224 -0.81 225 225
72 0.57 21 0.54 42 1.00 12 20
73 0.29 237 0.41 237 -1.47 237 237
74 0.53 51 0.54 38 0.13 99 53
75 0.45 174 0.54 61 -0.26 169 136
76 0.52 69 0.50 142 0.04 114 112
77 0.51 85 0.51 133 0.12 100 109
78 0.45 173 0.49 181 -0.17 155 172
79 0.52 72 0.52 118 0.16 92 84
80 0.40 219 0.45 222 -0.77 223 221
81 0.37 228 0.42 234 -0.75 221 229
82 0.54 42 0.51 124 0.11 101 82
83 0.42 211 0.47 198 -0.64 212 213
84 0.47 152 0.50 152 0.02 117 147
85 0.49 133 0.50 158 -0.08 136 150
86 0.45 179 0.45 215 -0.10 137 182
87 0.21 239 0.38 239 -1.99 239 239
88 0.56 31 0.54 59 0.44 48 41
89 0.53 63 0.52 96 0.52 40 57
90 0.45 172 0.49 169 -0.15 149 166
91 0.43 202 0.46 209 -0.22 164 201
92 0.50 101 0.53 74 0.32 69 73
93 0.51 90 0.51 139 0.35 61 89
94 0.52 77 0.56 22 0.42 55 45
95 0.47 156 0.49 165 -0.13 144 160
96 0.55 33 0.56 16 0.43 49 31
97 0.52 75 0.53 70 0.04 115 77
98 0.53 54 0.53 76 0.54 37 47
99 0.50 102 0.54 45 0.15 96 71
100 0.38 226 0.45 219 -0.92 231 227
101 0.45 171 0.50 161 -0.43 192 180
102 0.57 25 0.53 72 0.90 17 34
103 0.45 176 0.51 131 -0.35 183 167
104 0.53 62 0.50 143 0.15 95 95
105 0.42 212 0.47 205 -0.68 216 216
106 0.55 36 0.52 110 0.76 26 48
107 0.59 13 0.56 15 0.97 14 13
108 0.50 93 0.53 79 -0.15 150 111
109 0.54 44 0.54 53 0.43 50 44
110 0.51 88 0.51 136 0.20 83 101
111 0.48 137 0.50 148 -0.04 127 142
112 0.49 119 0.54 60 -0.04 129 102
113 0.52 79 0.50 159 0.37 59 91
114 0.52 80 0.53 64 0.20 84 64
115 0.49 125 0.52 97 0.27 75 92
116 0.49 131 0.51 127 -0.19 162 146
117 0.52 74 0.53 85 0.40 57 61
118 0.47 151 0.52 92 -0.16 153 133
119 0.49 121 0.52 117 -0.01 120 127
120 0.45 180 0.49 180 -0.51 197 193
121 0.50 99 0.52 95 0.08 107 96
122 0.42 207 0.47 201 -0.65 213 214
122 0.42 207 0.47 201 -0.65 213 214
123 0.38 225 0.43 227 -0.62 208 219
124 0.47 159 0.50 155 -0.40 189 171
125 0.44 190 0.46 207 -0.19 161 195
126 0.53 65 0.51 140 0.42 54 75
127 0.44 189 0.48 190 -0.63 209 207
128 0.51 87 0.52 109 -0.05 131 114
129 0.39 221 0.44 225 -0.77 224 224
130 0.42 208 0.51 135 -0.44 193 185
131 0.42 210 0.49 176 -0.17 156 188
132 0.40 217 0.46 210 -0.76 222 217
133 0.61 7 0.56 21 0.97 13 10
134 0.54 47 0.54 51 0.51 42 42
135 0.43 200 0.48 195 -0.36 184 203
136 0.41 214 0.45 220 -0.90 230 222
137 0.42 205 0.48 192 -0.71 217 211
138 0.50 111 0.53 88 0.10 102 97
139 0.50 94 0.54 56 -0.11 139 88
140 0.51 82 0.55 34 0.17 91 59
141 0.49 126 0.52 103 0.13 97 113
142 0.58 19 0.55 24 0.72 27 17
143 0.49 123 0.51 128 -0.04 128 129
144 0.50 109 0.53 87 -0.04 125 110
145 0.47 146 0.52 112 -0.17 159 145
146 0.55 39 0.57 10 0.80 22 18
147 0.44 198 0.49 182 -0.55 202 204
148 0.55 37 0.56 17 0.52 41 30
149 0.39 220 0.44 226 -0.81 226 226
150 0.49 129 0.52 116 -0.25 168 143
151 0.50 96 0.50 153 0.08 106 125
152 0.49 132 0.50 162 -0.07 134 151
153 0.50 113 0.52 120 0.07 109 120
154 0.49 130 0.50 154 -0.24 166 155
155 0.43 201 0.45 217 -0.65 214 215
156 0.49 128 0.52 119 0.17 90 117
157 0.59 16 0.57 6 0.66 30 15
158 0.57 20 0.56 23 0.60 33 22
159 0.56 26 0.56 20 0.40 58 32
160 0.50 95 0.54 39 0.10 103 68
161 0.45 183 0.48 184 -0.45 194 198
162 0.63 6 0.56 11 1.25 5 6
163 0.44 195 0.50 149 -0.46 195 187
164 0.56 28 0.55 29 0.89 18 21
165 0.49 117 0.46 211 0.71 28 126
166 0.35 232 0.42 230 -1.29 235 233
167 0.46 170 0.51 130 -0.49 196 170
168 0.49 135 0.53 69 -0.04 126 115
169 0.59 12 0.57 8 0.83 20 9
170 0.53 56 0.54 50 0.30 70 49
171 0.50 114 0.50 150 0.23 79 121
172 0.47 158 0.53 77 -0.26 170 137
173 0.52 73 0.49 173 0.35 65 104
174 0.53 64 0.54 43 0.30 71 50
175 0.51 81 0.51 129 0.43 51 79
176 0.58 18 0.57 5 0.54 36 16
177 0.53 55 0.55 36 0.15 94 51
178 0.49 115 0.52 93 0.10 104 105
179 0.44 191 0.52 108 -0.64 211 173
180 0.68 1 0.58 4 2.16 2 2
181 0.52 66 0.52 107 0.41 56 65
182 0.57 23 0.55 31 0.79 23 23
183 0.52 70 0.51 125 0.32 68 80
184 0.44 193 0.47 200 -0.12 142 184
185 0.68 2 0.60 2 1.12 7 4
186 0.53 53 0.51 126 0.93 16 55
187 0.48 142 0.49 171 -0.26 171 163
188 0.54 45 0.49 179 0.86 19 72
189 0.48 139 0.48 187 -0.05 130 157
190 0.37 229 0.45 221 -1.19 233 230
191 0.17 240 0.37 240 -2.14 240 240
192 0.53 61 0.52 115 0.46 45 63
193 0.38 224 0.43 229 -0.63 210 220
194 0.41 216 0.45 218 -0.14 148 205
195 0.48 145 0.53 78 0.28 74 93
196 0.44 186 0.47 202 -0.28 173 199
197 0.51 86 0.48 188 0.51 43 108
198 0.49 118 0.50 157 0.04 112 132
199 0.67 3 0.62 1 2.21 1 1
200 0.46 165 0.46 213 -0.28 175 190
201 0.50 97 0.52 91 0.18 89 83
202 0.45 178 0.48 193 -0.39 187 196
203 0.60 9 0.56 18 1.03 10 8
204 0.60 8 0.53 68 1.34 4 24
205 0.46 161 0.49 177 -0.28 174 176
206 0.36 230 0.42 233 -0.89 229 232
207 0.59 15 0.56 12 1.04 9 7
208 0.56 30 0.55 37 0.82 21 27
209 0.45 185 0.45 216 -0.23 165 200
210 0.49 116 0.50 146 -0.14 147 139
211 0.59 17 0.56 19 1.01 11 14
212 0.47 149 0.47 203 -0.17 158 174
213 0.47 153 0.46 214 -0.02 123 168
214 0.56 29 0.55 28 0.56 34 28
215 0.67 4 0.60 3 1.85 3 3
216 0.43 199 0.47 199 -0.19 160 197
217 0.36 231 0.43 228 -0.75 220 228
218 0.52 67 0.52 90 0.20 85 69
219 0.50 105 0.51 122 0.29 72 94
220 0.45 181 0.49 168 0.26 76 149
221 0.54 48 0.54 44 0.43 52 43
222 0.50 112 0.49 172 0.24 78 128
223 0.57 22 0.54 46 0.79 25 29
224 0.56 27 0.54 48 0.67 29 33
225 0.50 91 0.51 138 0.35 66 90
226 0.48 136 0.50 151 0.04 113 135
227 0.55 40 0.52 111 0.35 62 60
228 0.48 144 0.48 189 -0.03 124 159
229 0.60 10 0.54 54 1.07 8 19
230 0.52 76 0.51 134 0.16 93 98
231 0.46 166 0.48 196 -0.06 132 169
232 0.44 188 0.50 160 -0.41 190 186
233 0.50 100 0.47 204 0.25 77 130
234 0.53 57 0.49 170 0.63 32 76
235 0.37 227 0.42 231 -0.87 228 231
236 0.55 38 0.55 35 0.48 44 36
237 0.55 32 0.52 113 0.42 53 56
238 0.54 41 0.54 41 0.44 47 38
239 0.53 59 0.53 71 0.19 87 62
240 0.33 234 0.53 80 -0.57 205 177
241 0.34 233 0.42 232 -1.18 232 234

表7

供试油菜种质各指标TC值与T值和WTC值的关联度及各指标权重"

指标
Index		 等权关联度γT 权重系数
ωi		 加权关联度γWTC
数值 Value 排序 Order 数值 Value 排序 Order
发芽率 GR 0.649 3 0.163 0.669 5
发芽势 GV 0.634 4 0.159 0.671 4
鲜重 FW 0.658 2 0.165 0.745 2
干重 DW 0.633 5 0.159 0.615 6
根长 RL 0.781 1 0.196 0.763 1
芽长 SL 0.630 6 0.158 0.673 3

表8

供试油菜种质苯磺隆耐性评价指标的分级"

指标
Index		 隶属函数值 Subordinate function value
I II III V IV
发芽率 GR 0.94 0.94 0.93 0.84 0.48
发芽势 GV 0.98 0.96 0.92 0.83 0.43
鲜重 FW 0.71 0.28 0.21 0.16 0.15
干重 DW 0.50 0.43 0.41 0.35 0.22
根长 RL 0.44 0.29 0.15 0.11 0.09
芽长 SL 0.40 0.47 0.40 0.32 0.28
ASF值 ASF value 0.67 0.57 0.50 0.43 0.28
WTC值 WTC value 0.60 0.54 0.52 0.48 0.40
T值 T value 2.08 0.77 0.12 -0.47 -1.63

表9

供试油菜种质耐性模型预测"

因变量
Dependent variable		 多元逐步回归方程
Multiple stepwise regression equation		 相关系数
r		 F值
F-value		 P值
P-value
ASF值 ASF value y = -0.4505+0.6436x1+0.1260x3+0.4108x5+0.1421x6 0.9555 620 0.0001
WTC值 WTC value y = -0.0506+0.2568x1+0.1867x2+0.3401x3-0.0606x4+0.0910x5+0.0320x6 0.9578 433 0.0001
T值 T value y = -6.5511+4.5564x1+1.5790x3+5.2021x5 0.9247 466 0.0001
[1] 张朝贤, 胡祥恩, 钱益新 . 国外除草剂应用趋势及我国杂草科学研究现状和发展方向. 植物保护学报, 1997,24:278-282
Zhang C X, Hu X E, Qian Y X . Trend of herbicides use in developed countries and current research and future directions in weed science research in China. J Plant Prot, 1997,24:278-282 (in Chinese with English abstract)
[2] 张朝贤, 倪汉文, 魏守辉, 黄红娟, 刘延, 崔海兰, 隋标峰, 张猛, 郭峰 . 杂草抗药性研究进展. 中国农业科学, 2009,42:1274-1289
Zhang C X, Ni H W, Wei S H, Huang H J, Liu Y, Cui H L, Sui B F, Zhang M, Guo F . Current advances in research on herbicide resistance. Sci Agric Sin, 2009,42:1274-1289 (in Chinese with English abstract)
[3] 吴春华, 陈欣 . 农药对农区生物多样性的影响. 应用生态学报, 2004,15:341-344
Wu C H, Chen X . Impact of pesticides on biodiversity in agricultural areas. Chin J Appl Ecol, 2004,15:341-344 (in Chinese with English abstract)
[4] 单正军, 陈祖义 . 除草剂对非靶植物(农作物)的危害影响及控制技术. 农药科学与管理, 2007,28(9):50-54
doi: 10.3969/j.issn.1002-5480.2007.09.017
Shan Z J, Chen Z Y . Harm and control technology of herbicides to non target plants (crops). Pestic Sci Admin, 2007,28(9):50-54 (in Chinese with English abstract)
doi: 10.3969/j.issn.1002-5480.2007.09.017
[5] Brighenti A M, Moraes V J, Oliveira Jr R S, Gazziero D L P, Voll E, Gomes J A . Persistência e fitotoxicidade do herbicida atrazine aplicado na cultura do milho sobre a cultura do girassol em sucessão. Planta Daninha, 2002,20:291-297
doi: 10.1590/S0100-83582002000200016
[6] 林长福 . 玉米田化学除草现状及发展趋势. 农药, 1999, ( 9):3-4
Lin C F . Present situation and development trend of chemical weed control in maize field. Agrochemicals, 1999, ( 9):3-4 (in Chinese with English abstract)
[7] 王汉中 . 我国油菜产需形势分析及产业发展对策. 中国油料作物学报, 2007,29:101-105
Wang H Z . Strategy for rapeseed industry development based on the analysis of rapeseed production and demand in China. Chin J Oil Crop Sci, 2007,29:101-105 (in Chinese with English abstract)
[8] Zhou W J, Yoneyama K, Takeuchi Y, Iso S, Rungmekarat S, Chae S H, Sato D, Joel D M . In vitro infection of host roots by differentiated calli of the parasitic plant orobanche. J Exp Bot, 2004,55:899-907
doi: 10.1093/jxb/erh098
[9] Song W J, Zhou W J, Jin Z L, Gao D D, Joel D M, Takeuchi Y, Yoneyama K . Germination response of Orobanche seeds subjected to conditioning temperature, water potential and growth regulator treatments. Weed Res, 2005,45:467-476
doi: 10.1111/wre.2005.45.issue-6
[10] 俞琦英, 周伟军 . 油菜田的杂草发生特点及其防治研究概况. 浙江农业科学, 2010, ( 1):123-127
doi: 10.3969/j.issn.0528-9017.2010.01.048
Yu Q Y, Zhou W J . Study on weed occurrence characteristics and control in rape field. J Zhejiang Agric Sci, 2010, ( 1):123-127 (in Chinese)
doi: 10.3969/j.issn.0528-9017.2010.01.048
[11] 张宏军, 贾富勤, 张佳, 李晓晶 . 杂草对灭生性除草剂百草枯的抗性问题. 农药科学管理, 2003,24(12):26-29
doi: 10.3969/j.issn.1002-5480.2003.12.013
Zhang H J, Jia F L, Zhang J, Li X J . The resistant weeds of no selective herbicide-paraquat. Pestic Sci Admini, 2003,24(12):26-29 (in Chinese with English abstract)
doi: 10.3969/j.issn.1002-5480.2003.12.013
[12] 张宏军, 刘学, 张佳, 崔海兰, 张朝贤, 朱文达 . 我国油菜田除草剂登记和使用情况. 科技创新导报, 2008, ( 15):252-253
doi: 10.3969/j.issn.1674-098X.2008.15.201
Zhang H J, Liu X, Zhang J, Cui H L, Zhang C X, Zhu W D . Herbicide registration and usage in rape fields in China. Sci Technol Innov Herald, 2008, ( 15):252-253 (in Chinese)
doi: 10.3969/j.issn.1674-098X.2008.15.201
[13] 孙妍妍, 曲高平, 黄谦心, 吕金洋, 郭媛, 胡胜武 . 甘蓝型油菜耐苯磺隆突变体ALS基因分析与SNP标记. 中国油料作物学报, 2015,37:589-595
Sun Y Y, Qu G P, Huang Q X, Lyu J Y, Guo Y, Hu S W . SNP markers for acetolactate synthase genes from tribenuron-methyl resistant mutants in Brassica napus L. Chin J Oil Crop Sci, 2015,37:589-595 (in Chinese with English abstract)
[14] 信晓阳, 曲高平, 张荣, 庞红喜, 吴强, 王发禄, 胡胜武 . 不同品种油菜对苯磺隆耐药性差异的鉴定. 西北农业学报, 2014,23(7):68-74
doi: 10.7606/j.issn.1004-1389.2014.07.012
Xin X Y, Qu G P, Zhang R, Pang H X, Wu Q, Wang F L, Hu S W . Identification of the tribenuron-methyl tolerance in different rapeseed genotypes. Acta Agric Boreali-Occident Sin, 2014,23(7):68-74 (in Chinese with English abstract)
doi: 10.7606/j.issn.1004-1389.2014.07.012
[15] Yu C, Hu S, He P, Sun G, Zhang C, Yu Y . Inducing male sterility in Brassica napus L. by a sulphonylurea herbicide, tribenuron-methyl. Plant Breed, 2006,125:61-64
doi: 10.1111/j.1439-0523.2006.01180.x
[16] Li H, Li J, Zhao B, Wang J, Yi L, Liu C, Wu J, King G J, Liu K . Generation and characterization of tribenuron-methyl herbicide-resistant rapeseed (Brasscia napus) for hybrid seed production using chemically induced male sterility. Theor Appl Genet, 2015,128:107-118
[17] 吴学莉, 易丽聪, 侯凡, 吴江生, 姚璇, 刘克德 . 表达播娘蒿突变基因DsALS-108的抗苯磺隆甘蓝型油菜植株构建. 农业生物技术学报, 2016,24:469-477
doi: 10.3969/j.issn.1674-7968.2016.04.001
Wu X L, Yi L C, Hou F, Wu J S, Yao X, Liu K D . Generation of tribenuron-methyl herbicide resistant rapeseed (Brasscia napus) plants expressing mutated gene DsALS-108 of flixweed(Descurainia sophia). J Agric Biotechnol, 2016,24:469-477 (in Chinese with English abstract)
doi: 10.3969/j.issn.1674-7968.2016.04.001
[18] 曲高平, 孙妍妍, 庞红喜, 吴强, 王发禄, 胡胜武 . 甘蓝型油菜EMS突变体库构建及抗除草剂突变体筛选. 中国油料作物学报, 2014,36:25-31
doi: 10.7505/j.issn.1007-9084.2014.01.004
Qu G P, Sun Y Y, Pang H X, Wu Q, Wang F L, Hu S W . Ems mutagenesis and als-inhibitor herbicide-resistant mutants of Brassica napus L. Chin J Oil Crop Sci, 2014,36:25-31 (in Chinese with English abstract)
doi: 10.7505/j.issn.1007-9084.2014.01.004
[19] 汪亚琴 . 水稻抗除草剂基因CYP81A6转化油菜的研究. 华中农业大学硕士学位论文, 湖北武汉, 2013
Wang Y Q . The Expression of Rice Herbicide Resistance Gene CYP81A6 in Brassica napus. MS Thesis of Huazhong Agricultural University, Wuhan, Hubei, China, 2013 ( in Chinese with English abstract)
[20] 邱芳心, 杜桂萍, 刘开林, 毛爱星, 罗坤 . 杂草抗药性及其治理策略研究进展. 杂草科学, 2015, ( 2):1-6
doi: 10.3969/j.issn.1003-935X.2015.02.001
Qiu F X, Du G P, Liu K L, Mao A X, Luo K . Research progress on weed resistance to herbicides and control methods. Weed Sci, 2015, ( 2):1-6 (in Chinese with English abstract)
doi: 10.3969/j.issn.1003-935X.2015.02.001
[21] 信晓阳 . 油菜苯磺隆抗性研究与转基因抗除草剂油菜的选育. 西北农林科技大学硕士学位论文, 陕西西安, 2013
Xin X Y . Charaterization of Resistance to Tribenuron-Methyl in Rapeseed (Brassica napus L.) and Development of Transgenic Rapeseed with Herbericide-Resistance. MS Thesis of Northwest A&F University, Xi’an, Shaanxi, China, 2013 ( in Chinese with English abstract)
[22] Koger C H, Poston D H, Hayes R M, Montgomery R F . Glyphosate-resistant horseweed (Conyza canadensis) in Mississippi. Weed Technol, 2004,189:820-825
[23] Kuk Y I, Kim K H, Kwon O D, Lee D J, Burgos N R, Jung S, Guh J O . Cross-resistance pattern and alternative herbicides for Cyperus difformis resistant to sulfonylurea herbicides in Korea. Pest Manage Sci, 2004,60:85-94
doi: 10.1002/(ISSN)1526-4998
[24] 刘伟, 王金信, 杨广玲, 毕建杰, 隋标峰 . 不同小麦品种对苯磺隆耐药性差异及其机理. 植物保护学报, 2005,32:300-304
Liu W, Wang J X, Yang G L, Bi J J, Sui B F . Different of tolerance and mechanism of various wheat varieties to tribenuron-methyl. Acta Phytophy Sin, 2005,32:300-304 (in Chinese with English abstract)
[25] Heap I M . International survey of herbicide-resistant weeds. Weed Technol, 1990,4(1):220
doi: 10.1017/S0890037X00025252
[26] Deng W, Yang Q, Jiao H T, Zhang Y Z, Li X F, Zhang M Q . Cross-resistance pattern to four AHAS-inhibiting herbicides of tribenuron-methyl-resistant flixweed (Descurainia sophia) conferred by Asp-376-Glu mutation in AHAS. J Integr Agric, 2016,15:2563-2570
[27] Sun J, Wang J X, Zhang H J, Liu J L, Bian S N . Study on mutations in ALS for resistance to tribenuron-methyl in Galium aparine L. Agric Sci Chin, 2011,10:86-91
[28] Cumminsa I, Wortleyb David J, Sabbadin F, Heb Z, Coxona C R, Strakera H E, Sellarsa J D, Knighta K, Edwardsc L, Hughesd D, Kaundund S S, Hutchingsd S J, Steela P G, Edwardsb R . Key role for a glutathione transferase in multiple-herbicide resistance in grass weeds. Proc Natl Acad Sci USA, 2013,110:5812-5817
doi: 10.1073/pnas.1221179110 pmid: 23530204
[29] 商璐 . 抗草甘膦大豆种质挖掘及抗性机制研究. 东北农业大学硕士学位论文, 黑龙江哈尔滨, 2016
Shang L . Study on Screening and Resistant Mechanism of Germplasm Resources to Glyphosate-Resistance in Soybean. MS Thesis of Northeast Agricultural University, Harbin, Heilongjiang, China, 2016 (in Chinese with English abstract)
[30] Kotoula-Syka E, Eleftherohorinos I G, Gagianas A A, Sficas A G . Phytotoxicity and persistence of chlorsulfuron, metsulfuron- methyl, triasulfuron and tribenuron-methyl in three soils. Weed Res, 2010,33:355-367
[31] 邹月利, 陶波 . 磺酰脲类除草剂的降解机制及代谢产物的研究进展. 农药科学与管理, 2011,32(10):24-31
Zou Y L, Tao B . Research advance on the degradation mechanism and degradation products of sulfonylurea herbicides. Pestic Sci Admini, 2011,32(10):24-31 (in Chinese with English abstract)
[32] 王正贵 . 除草剂对小麦产量和品质的影响及其残留特性. 扬州大学博士学位论文, 江苏扬州, 2011
doi: 10.7666/d.y2050365
Wang Z G . Effects of Herbicides on Grain Yield and Quality in Wheat and Relevant Residual Behavior. PhD Dissertation of Yangzhou University, Yangzhou, Jiangsu, China, 2011 ( in Chinese with English abstract)
doi: 10.7666/d.y2050365
[33] 江改青 . 小麦和土壤中苯磺隆与氯氟吡氧乙酸残留分析方法及消解动态研究. 安徽农业大学硕士学位论文, 安徽合肥, 2009
doi: 10.7666/d.y1597372
Jiang G Q . Study on Analytical Mechods and Dynamics of Tribenuron-methyI and Fluroxypyr Residues in Wheat and Soil. MS Thesis of Anhui Agricultural University, Hefei, Hanhui, China, 2009 ( in Chinese with English abstract)
doi: 10.7666/d.y1597372
[34] 陈新, 张宗文, 吴斌 . 裸燕麦萌发期耐盐性综合评价与耐盐种质筛选. 中国农业科学, 2014,47:2038-2046
Chen X, Zhang Z W, Wu B . Comprehensive evaluation of salt tolerance and screening for salt tolerant accessions of naked oat (Avena nuda L.) at germination stage. Sci Agric Sin, 2014,47:2038-2046 (in Chinese with English abstract)
[35] 汪梦竹, 慕小倩, 李玉菲, 崔宏安, 郭媛, 胡胜武 . 油菜和小麦种苗根系对乙草胺的耐性差异分析. 植物保护学报, 2017,44:337-342
doi: 10.13802/j.cnki.zwbhxb.2017.2015145
Wang M Z, Mu X Q, Li Y F, Cui H A, Guo Y, Hu S W . Analysis of acetochlor tolerance in root of Brassica napus L. and Triticum aestivum L. J Plant Prot, 2017,44:337-342 (in Chinese with English abstract)
doi: 10.13802/j.cnki.zwbhxb.2017.2015145
[36] 刘安芳, 伍莲 . 生物统计学. 重庆: 西南师范大学出版社, 2013. pp 279-285
Liu A F, Wu L. Biostatistics. Chongqing: Southwest China Normal University Press, 2013. pp 279-285(in Chinese)
[37] 唐启义, 冯明光 . DPS数据处理系统: 实验设计统计分析及数据挖掘. 北京: 科学出版社, 2007. pp 636-644, 682-690, 1027-1036
Tang Q Y, Feng M G. DPS Data Processing System: Statistical Analysis and Data Mining of Experimental Design. Beijing: Science Press, 2007. pp 636-644, 682-690, 1027-1036(in Chinese)
[38] 闫锋, 崔秀辉, 李清泉, 王成, 曾玲玲, 刘峰, 马波, 袁明 . 绿豆品种的灰色关联度分析及综合评价. 中国种业, 2011, ( 增刊2):31-33
Yan F, Cui X H, Li Q Q, Wang C, Zeng L L, Liu F, Ma B, Yuan M . Grey relational grade analysis and comprehensive evaluation of mungbean (Vigna radiata L.) germplasm resources. Chin Seed Ind, 2011, ( suppl-2):31-33 (in Chinese with English abstract)
[39] Upadhyaya Hari D . Variability for drought resistance related traits in the mini core collection of peanut. Crop Sci, 2005,45:1432-1440
doi: 10.2135/cropsci2004.0389
[40] 汪灿, 周棱波, 张国兵, 张立异, 徐燕, 高旭, 姜讷, 邵明波 . 薏苡种质资源萌发期抗旱性鉴定及抗旱指标筛选. 植物遗传资源学报, 2017,18:846-859
doi: 10.13430/j.cnki.jpgr.2017.05.006
Wang C, Zhou L B, Zhang G B, Zhang L Y, Xu Y, Gao X, Jiang N, Shao M B . Identification and indices screening of drought resistance in Job’s tears germplasm resources at germination stage. J Plant Genet Resour, 2017,18:846-859 (in Chinese with English abstract)
doi: 10.13430/j.cnki.jpgr.2017.05.006
[41] 于泉林, 武宝悦 . 不同作物对苯磺隆残留敏感性室内模拟研究. 河北职业技术师范学院学报, 2003,17(1):16-19
Yu Q L, Wu B Y . Different crops on the sensitivity of the indoor simulation of tribenuron-methyl residues. J Hebei Normal Univ Sci Technol, 2003,17(1):16-19 (in Chinese)
[42] 牛志锋, 杜慧玲 . 不同小麦品种对苯磺隆除草剂的耐药性研究. 山西农业科学, 2008,36(2):28-29
doi: 10.3969/j.issn.1002-2481.2008.02.011
Niu Z F, Du H L . Study on tolerance of different wheat variety to tribenuron-methyl. J Shanxi Agric Sci, 2008,36(2):28-29 (in Chinese with English abstract)
doi: 10.3969/j.issn.1002-2481.2008.02.011
[43] 娄国强, 吕文彦, 职明星 . 苯磺隆、苄嘧磺隆对不同小麦品种安全性及叶绿素含量的影响. 中国农学通报, 2005,21(10):317-320
doi: 10.3969/j.issn.1000-6850.2005.10.089
Lou G Q, Lyu W Y, Zhi M X . Studies on safety tribenuron- methyl and bensulfuron-methyl and their impact to the content of chlorophyll. Chin Agric Sci Bull, 2005,21(10):317-320 (in Chinese with English abstract)
doi: 10.3969/j.issn.1000-6850.2005.10.089
[44] 范志金, 钱传范, 于维强, 陈俊鹏, 李正名, 王玲秀 . 氯磺隆和苯磺隆对玉米乙酰乳酸合成酶抑制作用的研究. 中国农业科学, 2003,36:173-178
Fan Z J, Qian C F, Yu W Q, Chen J P, Li Z M, Wang L X . Study on enzymatic inhibition of acetolactate synthase from maize (Zea mays L.) by chlorsulfuron and tribenuron-methyl. Sci Agric Sin, 2003,36:173-178 (in Chinese with English abstract)
[45] 李脉泉, 化宿南, 郭兵福, 刘明, 宋健, 陈建港, 周福来, 于莉莉, 陶波, 邱丽娟 . 大豆微核心种质对草甘膦的耐受性鉴定. 植物遗传资源学报, 2015,16:940-946
doi: 10.13430/j.cnki.jpgr.2015.05.003
Li M Q, Hua S N, Guo B F, Liu M, Song J, Chen J G, Zhou F L, Yu L L, Tao B, Qiu L J . Identification of glyphosate-tolerance in soybean mini-core collection. J Plant Genet Resour, 2015,16:940-946 (in Chinese with English abstract)
doi: 10.13430/j.cnki.jpgr.2015.05.003
[46] 王园园 . 棉花草甘膦自然抗性评价及抗性基因源挖掘研究. 中国农业科学院博士学位论文, 北京, 2015
doi: 10.7666/d.Y2787485
Wang Y Y . Identification of Natural Resistance to Glyphosate in Gossypium and the Excavation of Glyphosate-resistant Gene Resources in Gossypium hirsutum Races. PhD Dissertation of Chinese Academy of Agricultural Sciences, Beijing, China, 2015 (in Chinese with English abstract)
doi: 10.7666/d.Y2787485
[47] 苏少泉 . 抗咪唑啉酮类除草剂作物的发展与未来. 现代农药, 2006,5(1):1-4
Su S Q . The development and future of imidazolinone herbicide-resistant crops. Mod Agrochem, 2006,5(1):1-4 (in Chinese)
[48] 王米道, 程凤侠, 司友斌 . 铜与草甘膦复合污染对小麦种子发芽与根伸长的抑制作用. 生态毒理学报, 2009,4:591-596
Wang M D, Cheng F X, Si Y B . The inhibition of the combined pollution of copper and glyphosate to the seed germination and root elongation of wheat. Asian J Ecotoxicol, 2009,4:591-596 (in Chinese with English abstract)
[49] 杜小娟, 梁婷婷, 慕小倩 . 8种常用除草剂对黄芩种子萌发及幼苗生长的影响. 西北农业学报, 2012,21:202-206
doi: 10.3969/j.issn.1004-1389.2012.04.040
Du X J, Liang T T, Mu X Q . Effects of eight herbicides on seed germination and seedling growth of Scutellaria baicalensis Georg. Acta Agric Boreali-Occident Sin, 2012,21:202-206 (in Chinese with English abstract)
doi: 10.3969/j.issn.1004-1389.2012.04.040
[1] 张钰坤, 陆赢, 崔看, 夏石头, 刘忠松. 芥菜种子颜色调控基因TT8的等位变异及其地理分布分析[J]. 作物学报, 2022, 48(6): 1325-1332.
[2] 陈松余, 丁一娟, 孙峻溟, 黄登文, 杨楠, 代雨涵, 万华方, 钱伟. 甘蓝型油菜BnCNGC基因家族鉴定及其在核盘菌侵染和PEG处理下的表达特性分析[J]. 作物学报, 2022, 48(6): 1357-1371.
[3] 秦璐, 韩配配, 常海滨, 顾炽明, 黄威, 李银水, 廖祥生, 谢立华, 廖星. 甘蓝型油菜耐低氮种质筛选及绿肥应用潜力评价[J]. 作物学报, 2022, 48(6): 1488-1501.
[4] 袁大双, 邓琬玉, 王珍, 彭茜, 张晓莉, 姚梦楠, 缪文杰, 朱冬鸣, 李加纳, 梁颖. 甘蓝型油菜BnMAPK2基因的克隆及功能分析[J]. 作物学报, 2022, 48(4): 840-850.
[5] 陈小红, 林元香, 王倩, 丁敏, 王海岗, 陈凌, 高志军, 王瑞云, 乔治军. 基于高基元SSR构建黍稷种质资源的分子身份证[J]. 作物学报, 2022, 48(4): 908-919.
[6] 黄成, 梁晓梅, 戴成, 文静, 易斌, 涂金星, 沈金雄, 傅廷栋, 马朝芝. 甘蓝型油菜BnAPs基因家族成员全基因组鉴定及分析[J]. 作物学报, 2022, 48(3): 597-607.
[7] 王瑞, 陈雪, 郭青青, 周蓉, 陈蕾, 李加纳. 甘蓝型油菜白花基因InDel连锁标记开发[J]. 作物学报, 2022, 48(3): 759-769.
[8] 胡亮亮, 王素华, 王丽侠, 程须珍, 陈红霖. 绿豆种质资源苗期耐盐性鉴定及耐盐种质筛选[J]. 作物学报, 2022, 48(2): 367-379.
[9] 宋丽君, 聂晓玉, 何磊磊, 蒯婕, 杨华, 郭安国, 黄俊生, 傅廷栋, 汪波, 周广生. 饲用大豆品种耐荫性鉴定指标筛选及综合评价[J]. 作物学报, 2021, 47(9): 1741-1752.
[10] 张鹤, 蒋春姬, 殷冬梅, 董佳乐, 任婧瑶, 赵新华, 钟超, 王晓光, 于海秋. 花生耐冷综合评价体系构建及耐冷种质筛选[J]. 作物学报, 2021, 47(9): 1753-1767.
[11] 王艳花, 刘景森, 李加纳. 整合GWAS和WGCNA筛选鉴定甘蓝型油菜生物产量候选基因[J]. 作物学报, 2021, 47(8): 1491-1510.
[12] 王琰琰, 王俊, 刘国祥, 钟秋, 张华述, 骆铮珍, 陈志华, 戴培刚, 佟英, 李媛, 蒋勋, 张兴伟, 杨爱国. 基于SSR标记的雪茄烟种质资源指纹图谱库的构建及遗传多样性分析[J]. 作物学报, 2021, 47(7): 1259-1274.
[13] 李杰华, 端群, 史明涛, 吴潞梅, 柳寒, 林拥军, 吴高兵, 范楚川, 周永明. 新型抗广谱性除草剂草甘膦转基因油菜的创制及其鉴定[J]. 作物学报, 2021, 47(5): 789-798.
[14] 唐鑫, 李圆圆, 陆俊杏, 张涛. 甘蓝型油菜温敏细胞核雄性不育系160S花药败育的形态学特征和细胞学研究[J]. 作物学报, 2021, 47(5): 983-990.
[15] 周新桐, 郭青青, 陈雪, 李加纳, 王瑞. GBS高密度遗传连锁图谱定位甘蓝型油菜粉色花性状[J]. 作物学报, 2021, 47(4): 587-598.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备15008789号-2