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作物学报

作物学报 ›› 2018, Vol. 44 ›› Issue (8): 1169-1184.doi: 10.3724/SP.J.1006.2018.01169

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

甘蓝型油菜种子萌发期耐苯磺隆种质筛选与综合评价

王倩(),崔翠(),叶桑,崔明圣,赵愉风,林呐,唐章林,李加纳,周清元()   

  1. 西南大学农学与生物科技学院, 重庆400715
  • 收稿日期:2017-11-20 接受日期:2018-03-26 出版日期:2018-08-10 网络出版日期:2018-04-18
  • 通讯作者: 王倩,崔翠,周清元
  • 基金资助:
    国家现代农业产业技术体系建设专项(CARS-12);国家科技支撑计划项目(2013BAD01B03-12);重庆市社会事业与民生保障科技创新项目(cstc2016shmszx0756);西南大学博士启动基金项目(swu113064)

Screening and Comprehensive Evaluation of Germplasm Resources with Tribenuron-methyl Tolerance at Germination Stage in Rapeseed (Brassica napus L.)

Qian WANG(),Cui CUI(),Sang YE,Ming-Sheng CUI,Yu-Feng ZHAO,Na LIN,Zhang-Lin TANG,Jia-Na LI,Qing-Yuan ZHOU()   

  1. College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China
  • Received:2017-11-20 Accepted:2018-03-26 Published:2018-08-10 Published online:2018-04-18
  • Contact: Qian WANG,Cui CUI,Qing-Yuan ZHOU
  • Supported by:
    the China Agriculture Research System(CARS-12);the National Key Technology Support Program of China(2013BAD01B03-12);the Science and Technology Committee of Chongqing(cstc2016shmszx0756);the Doctoral Start-up Fund of Southwestern University(swu113064)

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摘要：

本研究旨在探讨不同基因型甘蓝型油菜种子萌发期耐除草剂苯磺隆特性, 建立其筛选的评价体系, 选育萌发期耐苯磺隆的甘蓝型油菜品种(系)。采用苯磺隆耐性度量值(T值)、平均隶属函数值(ASF值)、加权耐性系数(WTC值)等指标及相关分析、频数分析、主成分分析、灰色关联度分析、聚类分析和逐步回归分析相结合的方法, 鉴定萌发期耐性。通过11份供试材料相对根长的方差分析, 确定了油菜萌发期耐苯磺隆种质资源筛选及评价研究的最佳苯磺隆浓度为25 mg kg -1。在此浓度胁迫下, 241份甘蓝型油菜的根长、芽长、率、发芽势、鲜重5个指标均存在显著性差异。根据T值的聚类分析, 将供试种质划分为5个苯磺隆耐性级别, 其中I级3份、II级30份、III级198份、IV级6份、V级4份。对苯磺隆耐性较强的甘蓝型油菜品种(系)为希望106、SWU95和WH-33, 可作为甘蓝型油菜苯磺隆耐性育种和耐性机制研究的重要资源。根长、鲜重、发芽率可作为评价油菜种质资源萌发期耐性的指标性状。

关键词: 甘蓝型油菜, 耐苯磺隆, 种质资源, 综合评价, 萌发期

Abstract:

The purpose of this study is to explore the characteristics of tribenuron-methyl tolerance in different genotypes of Brassica napus, and establish the evaluation system for screening and breeding new cultivars with tribenuron-methyl tolerance. The germination vigor was measured on the third day after treatment and the germination rate, shoot length, root length, dry weight, as well as fresh weight were measured on the seventh day. The identification of tolerance at germination stage was performed by tribenuron-methyl tolerance comprehensive evaluation value (T), average subordinative function value (ASF) and weight tribenuron-methyl tolerance coefficient (WTC) in correlation analysis, frequency analysis, principal component analysis, grey analysis, cluster analysis and stepwise regression analysis. Based on the variance analysis of relative root length index in 11 tested materials, we determined the optimum treatment concentration (25 mg kg -1) for selecting and evaluating the tolerant germplasm resources to tribenuron-methyl in napeseed. Under the optimum concentration, the single factor variance analysis showed significant differences in root length, shoot length, germination rate, germination vigor and fresh weight among 241 accessions of Brassica napus. The clustering analysis according to T value exhibited that the tested cultivars were roughly divided into five tribenuron-methyl tolerance grades, including three in grade I, 30 in grade II, 198 in grade III, six in grade IV, and four in grade V. We conclude that the varieties (lines) with strong tribenuron-methyl tolerance at germination stage are Xiwang 106, SWU95, and WH-33, which could be used as the germplasm materials for the study on tolerance breeding and mechanism. Furthermore, the root length, fresh weight and germination rate could be used as index traits to evaluate the tolerance of rapeseed germplasm resources during germination stage.

Key words: Brassica napus L, Tribenuron-methyl tolerance, germplasm resources, comprehensive evaluation, germination stage

表1

不同浓度下11份品种根长相对值的显著性分析"

品种(系)
Variety (line)		 苯磺隆浓度 Concentration of tribenuron-methyl
0.25 mg kg-1 2.50 mg kg-1 25.00 mg kg-1 250.00 mg kg-1
炎81-2 Yan 81-2 70.3±0.007 c 17.8±0.015 de 10.4±0.003 f 10.3±0.003 de
SWU92 82.5±0.030 b 19.3±0.009 de 14.5±0.002 def 11.7±0.011 de
SWU101 64.5±0.077 cd 15.3±0.017 de 12.5±0.001 ef 9.3±0.012 e
782 48.5±0.003 e 18.5±0.004 de 13.5±0.006 ef 12.9±0.002 cde
509 50.0±0.008 e 19.8±0.021 de 15.4±0.004 cdef 14.5±0.009 bcde
P18 106.3±0.091 a 36.0±0.031 a 31.1±0.002 a 28.8±0.016 a
甲预31棚 Jiayu 31 peng 24.6±0.010 f 22.4±0.013 cd 19.6±0.029 bcd 15.8±0.006 bcde
苏油1号 Suyou 1 95.7±0.039 a 26.1±0.008 bc 23.8±0.012 b 20.0±0.004 bc
至尊 Zhizun 85.2±0.002 b 29.3±0.030 ab 22.3±0.001 bc 21.4±0.019 ab
B265 96.4±0.044 a 31.1±0.054 ab 18.5±0.011 bcde 18.0±0.006 bcd
中双12 Zhongshuang 12 60.1±0.010 d 13.3±0.017 e 13.2±0.038 ef 13.5±0.059 cde

表2

苯磺隆胁迫下油菜萌发期各指标的变化"

项目
Item		 参数
Parameter		 根长
RL (cm)		 芽长
SL (cm)		 发芽势
GV (%)		 发芽率
GR (%)		 鲜重
FW (g)		 干重
DW (g)
对照
Control		 最大值 Max. 13.13 5.81 100.00 100.00 0.79 0.08
最小值 Min. 0.55 0.76 10.00 15.00 0.08 0.01
均值 Average 7.38** 2.27** 81.31** 87.94** 0.38** 0.04**
标准差 SE 1.61 1.24 19.87 14.47 0.11 0.01
变异系数 CV (%) 21.77 54.56 24.44 16.45 28.91 26.74
胁迫
Stress		 最大值 Max. 2.97 6.24 100.00 100.00 0.79 0.06
最小值 Min. 0.34 0.71 5.00 15.00 0.12 0.01
均值 Average 0.94** 2.37** 75.77** 83.22** 0.36** 0.04**
标准差 SE 0.38 1.30 21.47 16.66 0.10 0.01
变异系数 CV (%) 40.00 54.83 28.34 20.01 28.00 25.91
较对照变化 均值 Average -6.44 0.10 -5.54 -4.72 -0.02 0
Comparison with the control 变异系数 CV (%) 18.23 0.27 3.90 3.56 -0.91 -0.83

图1

241份甘蓝型油菜不同指标耐性系数(TC)的频次分布 RL: 根长; FW: 鲜重; GV: 发芽势; GR: 发芽率; SL: 芽长; DW: 干重。"

表3

供试油菜种质各指标的苯磺隆耐性系数"

参数
Parameter		 根长
RL		 芽长 发芽势 发芽率 鲜重 干重
SL GV GR FW DW
最大值 Max. 0.588 2.005 1.000 1.000 2.357 1.946
最小值 Min. 0.050 0.494 0.143 0.333 0.411 0.333
均值 Average 0.136** 1.052** 0.926** 0.942** 0.980** 1.055
标准差 SE 0.066 0.187 0.123 0.097 0.202 0.136
变异系数 CV (%) 48.950 17.800 13.269 10.276 20.593 12.856

表4

供试油菜种质各指标耐性系数的相关性"

指标
Index		 根长
RL		 芽长
SL		 鲜重
FW		 发芽率
GR		 发芽势
GV		 干重
DW
RL 1
SL 0.046 1
FW 0.042 0.215** 1
GR -0.150* -0.059 -0.018 1
GV -0.171** 0.031 0.022 0.687** 1
DW -0.128* 0.031 0.062 0.133* 0.097 1

表5

各综合指标的特征值、贡献率和主成分特征向量值"

主成分
Principal
component		 特征值
Eigen
value		 贡献率
Variance
contribution (%)		 累计贡献率
Accumulated variance
contribution (%)		 所测指标的特征向量值 Eigen vector of measured indicators
发芽率
GR		 发芽势GV 鲜重
FW		 根长
RL		 芽长
SL		 干重
DW
1 1.810 30.167 30.167 0.978 0.945 0.279 0.011 0.261 0.617
2 1.310 21.825 51.992 -0.358 -0.263 0.841 0.396 0.769 0.258
3 1.096 18.267 70.259 0.115 0.311 -0.263 0.904 0.195 -0.637
4 0.790 13.159 83.418 -0.044 -0.057 0.316 0.508 -0.755 0.323

表6

苯磺隆胁迫下油菜品种资源的ASF值、WTC值、T值和综合排序"

编号
Code		 ASF值 ASF value WTC值 WTC value T值 T value 综合排序
Final rank
数值Value 排序Order 数值Value 排序Order 数值Value 排序Order
1 0.55 35 0.55 30 0.28 73 40
2 0.46 167 0.53 67 -0.30 178 141
3 0.49 127 0.55 32 0.07 108 81
4 0.48 138 0.54 62 -0.16 154 124
5 0.44 192 0.50 164 -0.55 201 192
6 0.57 24 0.55 26 0.65 31 25
7 0.48 141 0.48 194 -0.29 177 175
8 0.42 213 0.46 208 -0.53 199 212
9 0.47 148 0.52 101 -0.29 176 148
10 0.54 49 0.55 33 0.55 35 35
11 0.47 147 0.52 114 -0.14 145 138
12 0.52 78 0.52 121 0.05 111 103
13 0.45 177 0.52 106 -0.62 207 165
14 0.51 84 0.53 84 0.23 80 74
15 0.42 206 0.52 100 -0.66 215 178
16 0.47 150 0.49 174 -0.01 121 153
17 0.47 154 0.50 145 -0.15 151 154
18 0.50 98 0.53 83 0.10 105 85
19 0.42 203 0.49 178 -0.72 218 209
20 0.50 110 0.52 99 0.13 98 100
21 0.45 184 0.50 144 -0.55 203 181
22 0.44 194 0.51 132 -0.52 198 179
23 0.44 196 0.48 183 -0.54 200 202
24 0.49 122 0.53 65 -0.14 146 116
25 0.46 164 0.50 163 -0.12 141 161
26 0.47 155 0.51 123 -0.30 179 158
27 0.40 218 0.46 212 -0.83 227 218
28 0.49 134 0.52 94 0.05 110 118
29 0.60 11 0.57 7 0.79 24 11
30 0.42 209 0.48 191 -0.60 206 210
31 0.52 68 0.54 57 0.02 118 70
32 0.53 58 0.52 98 0.22 81 67
33 0.59 14 0.56 13 0.96 15 12
34 0.48 143 0.52 104 -0.21 163 140
35 0.33 235 0.41 236 -1.28 234 235
36 0.16 241 0.36 241 -2.57 241 241
37 0.50 92 0.50 141 -0.01 119 123
38 0.25 238 0.38 238 -1.77 238 238
39 0.48 140 0.53 66 -0.10 138 122
40 0.47 157 0.54 49 -0.07 133 119
41 0.46 169 0.53 82 -0.43 191 152
42 0.50 107 0.53 63 0.03 116 86
43 0.41 215 0.49 166 -0.57 204 206
编号
Code		 ASF值 ASF value WTC值 WTC value T值 T value 综合排序
Final rank
数值Value 排序Order 数值Value 排序Order 数值Value 排序Order
44 0.46 162 0.50 147 -0.13 143 156
45 0.44 197 0.49 175 -0.39 186 194
46 0.49 120 0.54 47 -0.02 122 87
47 0.39 223 0.45 223 -0.74 219 223
48 0.51 89 0.54 40 0.35 64 54
49 0.54 43 0.53 81 0.35 63 52
50 0.53 52 0.55 25 0.21 82 46
51 0.42 204 0.47 206 -0.36 185 208
52 0.64 5 0.57 9 1.13 6 5
53 0.50 106 0.50 156 -0.07 135 134
54 0.50 108 0.51 137 0.36 60 99
55 0.44 187 0.48 186 -0.31 181 191
56 0.46 163 0.49 167 -0.17 157 164
57 0.45 182 0.47 197 -0.24 167 189
58 0.50 103 0.54 58 -0.15 152 106
59 0.46 168 0.48 185 -0.31 180 183
60 0.54 50 0.55 27 0.46 46 37
61 0.47 160 0.53 75 -0.33 182 144
62 0.51 83 0.53 86 0.33 67 66
63 0.32 236 0.42 235 -1.41 236 236
64 0.53 60 0.54 55 0.20 86 58
65 0.54 46 0.54 52 0.53 39 39
66 0.55 34 0.56 14 0.53 38 26
67 0.49 124 0.53 89 -0.27 172 131
68 0.50 104 0.53 73 -0.11 140 107
69 0.45 175 0.52 105 -0.39 188 162
70 0.52 71 0.52 102 0.18 88 78
71 0.39 222 0.44 224 -0.81 225 225
72 0.57 21 0.54 42 1.00 12 20
73 0.29 237 0.41 237 -1.47 237 237
74 0.53 51 0.54 38 0.13 99 53
75 0.45 174 0.54 61 -0.26 169 136
76 0.52 69 0.50 142 0.04 114 112
77 0.51 85 0.51 133 0.12 100 109
78 0.45 173 0.49 181 -0.17 155 172
79 0.52 72 0.52 118 0.16 92 84
80 0.40 219 0.45 222 -0.77 223 221
81 0.37 228 0.42 234 -0.75 221 229
82 0.54 42 0.51 124 0.11 101 82
83 0.42 211 0.47 198 -0.64 212 213
84 0.47 152 0.50 152 0.02 117 147
85 0.49 133 0.50 158 -0.08 136 150
86 0.45 179 0.45 215 -0.10 137 182
87 0.21 239 0.38 239 -1.99 239 239
88 0.56 31 0.54 59 0.44 48 41
89 0.53 63 0.52 96 0.52 40 57
90 0.45 172 0.49 169 -0.15 149 166
91 0.43 202 0.46 209 -0.22 164 201
92 0.50 101 0.53 74 0.32 69 73
93 0.51 90 0.51 139 0.35 61 89
94 0.52 77 0.56 22 0.42 55 45
95 0.47 156 0.49 165 -0.13 144 160
96 0.55 33 0.56 16 0.43 49 31
97 0.52 75 0.53 70 0.04 115 77
98 0.53 54 0.53 76 0.54 37 47
99 0.50 102 0.54 45 0.15 96 71
100 0.38 226 0.45 219 -0.92 231 227
101 0.45 171 0.50 161 -0.43 192 180
102 0.57 25 0.53 72 0.90 17 34
103 0.45 176 0.51 131 -0.35 183 167
104 0.53 62 0.50 143 0.15 95 95
105 0.42 212 0.47 205 -0.68 216 216
106 0.55 36 0.52 110 0.76 26 48
107 0.59 13 0.56 15 0.97 14 13
108 0.50 93 0.53 79 -0.15 150 111
109 0.54 44 0.54 53 0.43 50 44
110 0.51 88 0.51 136 0.20 83 101
111 0.48 137 0.50 148 -0.04 127 142
112 0.49 119 0.54 60 -0.04 129 102
113 0.52 79 0.50 159 0.37 59 91
114 0.52 80 0.53 64 0.20 84 64
115 0.49 125 0.52 97 0.27 75 92
116 0.49 131 0.51 127 -0.19 162 146
117 0.52 74 0.53 85 0.40 57 61
118 0.47 151 0.52 92 -0.16 153 133
119 0.49 121 0.52 117 -0.01 120 127
120 0.45 180 0.49 180 -0.51 197 193
121 0.50 99 0.52 95 0.08 107 96
122 0.42 207 0.47 201 -0.65 213 214
122 0.42 207 0.47 201 -0.65 213 214
123 0.38 225 0.43 227 -0.62 208 219
124 0.47 159 0.50 155 -0.40 189 171
125 0.44 190 0.46 207 -0.19 161 195
126 0.53 65 0.51 140 0.42 54 75
127 0.44 189 0.48 190 -0.63 209 207
128 0.51 87 0.52 109 -0.05 131 114
129 0.39 221 0.44 225 -0.77 224 224
130 0.42 208 0.51 135 -0.44 193 185
131 0.42 210 0.49 176 -0.17 156 188
132 0.40 217 0.46 210 -0.76 222 217
133 0.61 7 0.56 21 0.97 13 10
134 0.54 47 0.54 51 0.51 42 42
135 0.43 200 0.48 195 -0.36 184 203
136 0.41 214 0.45 220 -0.90 230 222
137 0.42 205 0.48 192 -0.71 217 211
138 0.50 111 0.53 88 0.10 102 97
139 0.50 94 0.54 56 -0.11 139 88
140 0.51 82 0.55 34 0.17 91 59
141 0.49 126 0.52 103 0.13 97 113
142 0.58 19 0.55 24 0.72 27 17
143 0.49 123 0.51 128 -0.04 128 129
144 0.50 109 0.53 87 -0.04 125 110
145 0.47 146 0.52 112 -0.17 159 145
146 0.55 39 0.57 10 0.80 22 18
147 0.44 198 0.49 182 -0.55 202 204
148 0.55 37 0.56 17 0.52 41 30
149 0.39 220 0.44 226 -0.81 226 226
150 0.49 129 0.52 116 -0.25 168 143
151 0.50 96 0.50 153 0.08 106 125
152 0.49 132 0.50 162 -0.07 134 151
153 0.50 113 0.52 120 0.07 109 120
154 0.49 130 0.50 154 -0.24 166 155
155 0.43 201 0.45 217 -0.65 214 215
156 0.49 128 0.52 119 0.17 90 117
157 0.59 16 0.57 6 0.66 30 15
158 0.57 20 0.56 23 0.60 33 22
159 0.56 26 0.56 20 0.40 58 32
160 0.50 95 0.54 39 0.10 103 68
161 0.45 183 0.48 184 -0.45 194 198
162 0.63 6 0.56 11 1.25 5 6
163 0.44 195 0.50 149 -0.46 195 187
164 0.56 28 0.55 29 0.89 18 21
165 0.49 117 0.46 211 0.71 28 126
166 0.35 232 0.42 230 -1.29 235 233
167 0.46 170 0.51 130 -0.49 196 170
168 0.49 135 0.53 69 -0.04 126 115
169 0.59 12 0.57 8 0.83 20 9
170 0.53 56 0.54 50 0.30 70 49
171 0.50 114 0.50 150 0.23 79 121
172 0.47 158 0.53 77 -0.26 170 137
173 0.52 73 0.49 173 0.35 65 104
174 0.53 64 0.54 43 0.30 71 50
175 0.51 81 0.51 129 0.43 51 79
176 0.58 18 0.57 5 0.54 36 16
177 0.53 55 0.55 36 0.15 94 51
178 0.49 115 0.52 93 0.10 104 105
179 0.44 191 0.52 108 -0.64 211 173
180 0.68 1 0.58 4 2.16 2 2
181 0.52 66 0.52 107 0.41 56 65
182 0.57 23 0.55 31 0.79 23 23
183 0.52 70 0.51 125 0.32 68 80
184 0.44 193 0.47 200 -0.12 142 184
185 0.68 2 0.60 2 1.12 7 4
186 0.53 53 0.51 126 0.93 16 55
187 0.48 142 0.49 171 -0.26 171 163
188 0.54 45 0.49 179 0.86 19 72
189 0.48 139 0.48 187 -0.05 130 157
190 0.37 229 0.45 221 -1.19 233 230
191 0.17 240 0.37 240 -2.14 240 240
192 0.53 61 0.52 115 0.46 45 63
193 0.38 224 0.43 229 -0.63 210 220
194 0.41 216 0.45 218 -0.14 148 205
195 0.48 145 0.53 78 0.28 74 93
196 0.44 186 0.47 202 -0.28 173 199
197 0.51 86 0.48 188 0.51 43 108
198 0.49 118 0.50 157 0.04 112 132
199 0.67 3 0.62 1 2.21 1 1
200 0.46 165 0.46 213 -0.28 175 190
201 0.50 97 0.52 91 0.18 89 83
202 0.45 178 0.48 193 -0.39 187 196
203 0.60 9 0.56 18 1.03 10 8
204 0.60 8 0.53 68 1.34 4 24
205 0.46 161 0.49 177 -0.28 174 176
206 0.36 230 0.42 233 -0.89 229 232
207 0.59 15 0.56 12 1.04 9 7
208 0.56 30 0.55 37 0.82 21 27
209 0.45 185 0.45 216 -0.23 165 200
210 0.49 116 0.50 146 -0.14 147 139
211 0.59 17 0.56 19 1.01 11 14
212 0.47 149 0.47 203 -0.17 158 174
213 0.47 153 0.46 214 -0.02 123 168
214 0.56 29 0.55 28 0.56 34 28
215 0.67 4 0.60 3 1.85 3 3
216 0.43 199 0.47 199 -0.19 160 197
217 0.36 231 0.43 228 -0.75 220 228
218 0.52 67 0.52 90 0.20 85 69
219 0.50 105 0.51 122 0.29 72 94
220 0.45 181 0.49 168 0.26 76 149
221 0.54 48 0.54 44 0.43 52 43
222 0.50 112 0.49 172 0.24 78 128
223 0.57 22 0.54 46 0.79 25 29
224 0.56 27 0.54 48 0.67 29 33
225 0.50 91 0.51 138 0.35 66 90
226 0.48 136 0.50 151 0.04 113 135
227 0.55 40 0.52 111 0.35 62 60
228 0.48 144 0.48 189 -0.03 124 159
229 0.60 10 0.54 54 1.07 8 19
230 0.52 76 0.51 134 0.16 93 98
231 0.46 166 0.48 196 -0.06 132 169
232 0.44 188 0.50 160 -0.41 190 186
233 0.50 100 0.47 204 0.25 77 130
234 0.53 57 0.49 170 0.63 32 76
235 0.37 227 0.42 231 -0.87 228 231
236 0.55 38 0.55 35 0.48 44 36
237 0.55 32 0.52 113 0.42 53 56
238 0.54 41 0.54 41 0.44 47 38
239 0.53 59 0.53 71 0.19 87 62
240 0.33 234 0.53 80 -0.57 205 177
241 0.34 233 0.42 232 -1.18 232 234

表7

供试油菜种质各指标TC值与T值和WTC值的关联度及各指标权重"

指标
Index		 等权关联度γT 权重系数
ωi		 加权关联度γWTC
数值 Value 排序 Order 数值 Value 排序 Order
发芽率 GR 0.649 3 0.163 0.669 5
发芽势 GV 0.634 4 0.159 0.671 4
鲜重 FW 0.658 2 0.165 0.745 2
干重 DW 0.633 5 0.159 0.615 6
根长 RL 0.781 1 0.196 0.763 1
芽长 SL 0.630 6 0.158 0.673 3

表8

供试油菜种质苯磺隆耐性评价指标的分级"

指标
Index		 隶属函数值 Subordinate function value
I II III V IV
发芽率 GR 0.94 0.94 0.93 0.84 0.48
发芽势 GV 0.98 0.96 0.92 0.83 0.43
鲜重 FW 0.71 0.28 0.21 0.16 0.15
干重 DW 0.50 0.43 0.41 0.35 0.22
根长 RL 0.44 0.29 0.15 0.11 0.09
芽长 SL 0.40 0.47 0.40 0.32 0.28
ASF值 ASF value 0.67 0.57 0.50 0.43 0.28
WTC值 WTC value 0.60 0.54 0.52 0.48 0.40
T值 T value 2.08 0.77 0.12 -0.47 -1.63

表9

供试油菜种质耐性模型预测"

因变量
Dependent variable		 多元逐步回归方程
Multiple stepwise regression equation		 相关系数
r		 F值
F-value		 P值
P-value
ASF值 ASF value y = -0.4505+0.6436x1+0.1260x3+0.4108x5+0.1421x6 0.9555 620 0.0001
WTC值 WTC value y = -0.0506+0.2568x1+0.1867x2+0.3401x3-0.0606x4+0.0910x5+0.0320x6 0.9578 433 0.0001
T值 T value y = -6.5511+4.5564x1+1.5790x3+5.2021x5 0.9247 466 0.0001
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