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作物学报 ›› 2018, Vol. 44 ›› Issue (05): 783-789.doi: 10.3724/SP.J.1006.2018.00783

• 耕作栽培·生理生化 • 上一篇    

高粱种子萌发过程中超微弱光子辐射的特征

赵燕燕1,*(), 习岗2   

  1. 1郑州工业应用技术学院, 河南郑州 451100
    2西安理工大学理学院应用物理系, 陕西西安 710048
  • 收稿日期:2017-09-08 接受日期:2018-01-08 出版日期:2018-05-20 网络出版日期:2018-01-29
  • 通讯作者: 赵燕燕
  • 作者简介:

    第一作者联系方式: E-mail: anjp812@163.com

  • 基金资助:
    本研究由国家自然科学基金项目(31471412, 51277151)资助

Characteristics of Ultra-weak Photon Emission from Sorghum Seeds during Germination

Yan-Yan ZHAO1,*(), Gang XI2   

  1. 1 Zhengzhou University of Industrial Technology, Zhengzhou 451100, Henan, China
    2 Department of Applied Physics, Faculty of Sciences, Xi’an University of Technology, Xi’an 710048, Shaanxi, China;
  • Received:2017-09-08 Accepted:2018-01-08 Published:2018-05-20 Published online:2018-01-29
  • Contact: Yan-Yan ZHAO
  • Supported by:
    This study was supported by the National Natural Science Foundation of China (31471412, 51277151).

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摘要：

采用50 μg mL-1的蛋白质合成转录抑制剂放线菌素D (actinomycin D, AMD)、100 μg mL-1的蛋白质合成翻译抑制剂环己亚胺(cycloheximide, CHM)和650 μg mL-1的呼吸抑制剂NaN3处理萌发高粱种子, 研究了高粱种子萌发过程中自发光子辐射和LED诱导的延迟光子辐射的动力学特征及其变化规律。结果发现, 高粱萌发过程中的自发光子辐射强度与种子鲜质量的变化呈正相关(相关系数r为0.93121), AMD和CHM不同程度地抑制了萌发过程中高粱鲜质量的增长, NaN3几乎完全抑制了鲜质量的增长, 表明高粱萌发过程中的自发光子辐射强度可以作为萌发状况的信号, RNA/DNA合成反应是高粱萌发过程中自发光子辐射的来源之一, 呼吸代谢是其主要来源。高粱萌发过程中的延迟光子辐射特征可用动力学参数初始光子数、相干时间和延迟光子辐射积分强度定量表达, 通过对延迟光子辐射积分强度的测量和分析可以实时、灵敏和无损伤的了解和判断高粱萌发过程中细胞代谢系统的动态变化。

关键词: 高粱, 萌发, 超微弱光子辐射, 蛋白质合成抑制剂, 呼吸代谢抑制剂, 无损检测

Abstract:

Ultra-weak photon radiation from germinated seed includes spontaneous photon emission of germinated seeds in the dark and delayed photon radiation induced by external light. In order to study dynamical feature and biological significance of ultra-weak photon radiation from sorghum during germination, 50 μg mL-1 protein synthesis transcription inhibitor actinomycin D (AMD), 100 μg mL-1 protein synthesis translation inhibitor cycloheximide (CHM) and 650 μg mL-1 respiration inhibitor were adopted to treat germinated sorghum seeds separately. There was a positive correlation (correlation coefficient was 0.93121) between the spontaneous photon emission intensity of germinated sorghum and seed fresh mass. AMD and CHM partially inhibited while NaN3 almost completely inhibited the increase of fresh mass of sorghum seeds in germination process. It indicated that the spontaneous photon emission intensity from germinated sorghum seed can be taken as a signal of germination status from which RNA/DNA synthesis reaction is one of the sources and respiratory metabolism is the main source. The characteristic of delayed photon emission from germinated sorghum seeds can be expressed quantitatively by dynamical parameters such as initial photon number, coherence time and the integrated intensity of delayed photon emission. The cell metabolism and its changes in germination process of sorghum seeds can be understand and evaluated through a real-time, sensitive and non-destructive analysis of the integrated intensity of delayed photon emission from germinated sorghum seeds.

Key words: sorghum, germination, ultra-weak photon radiation, protein synthesis inhibitor, respiratory metabolic inhibitor, non-destructive test

图1

高粱萌发过程中鲜质量的变化"

图2

高粱萌发过程中自发光子辐射的变化"

表1

对照组萌发高粱延迟光子辐射动力学参数"

萌发时间Germination time
(d)		 自发光子辐射Spontaneous photon emission, ISL
(counts s-1)		 初始光子数
Initial photon number, I0
(counts s-1)		 相干时间
Coherence
time, τ
(s)		 衰减常量Attenuation
coefficient,
β		 拟合优度Determination
coefficient,
R2
0 7.64 237.28 0.26 0.84 0.993
1 8.57 256.05 0.27 0.87 0.992
2 9.51 298.72 0.30 0.95 0.996
3 10.38 333.00 0.32 0.96 0.992
4 10.39 337.77 0.35 0.97 0.995
5 10.17 323.95 0.32 0.96 0.991
6 10.41 344.55 0.34 0.97 0.995
7 10.27 331.29 0.33 0.96 0.996
8 10.42 353.76 0.38 0.97 0.996

表2

NaN3处理组萌发高粱延迟光子辐射动力学参数"

萌发时间
Germination time
(d)		 自发光子辐射Spontaneous photon emission, ISL
(counts s-1)		 初始光子数
Initial photon
number, I0
(counts s-1)		 相干时间
Coherence
time, τ
(s)		 衰减常量
Attenuation
coefficient,
β		 拟合优度
Determination
coefficient,
R2
0 7.64 234.28 0.25 0.83 0.992
1 7.78 245.65 0.26 0.84 0.996
2 7.96 269.56 0.27 0.86 0.993
3 8.02 278.51 0.28 0.87 0.991
4 7.56 233.87 0.23 0.81 0.991
5 7.16 228.73 0.22 0.78 0.991
6 7.27 229.76 0.23 0.80 0.994
7 7.16 223.19 0.21 0.80 0.993
8 7.18 226.09 0.22 0.78 0.996

表3

AMD处理组萌发高粱延迟光子辐射动力学参数"

萌发时间Germination time
(d)		 自发光子辐射Spontaneous photon emission, ISL
(counts s-1)		 初始光子数
Initial photon number, I0
(counts s-1)		 相干时间
Coherence
time, τ
(s)		 衰减常量Attenuation
coefficient,
β		 拟合优度Determination
coefficient,
R2
0 7.64 235.44 0.26 0.84 0.994
1 8.17 252.95 0.26 0.86 0.994
2 8.67 290.64 0.29 0.93 0.996
3 9.13 318.03 0.32 0.95 0.992
4 9.15 322.93 0.32 0.95 0.994
5 8.98 305.41 0.32 0.95 0.995
6 9.11 317.80 0.33 0.95 0.994
7 9.06 309.35 0.32 0.94 0.992
8 9.19 324.50 0.34 0.94 0.992

表4

CHM处理组萌发高粱延迟光子辐射动力学参数"

萌发时间Germination time
(d)		 自发光子辐射Spontaneous photon emission, ISL
(counts s-1)		 初始光子数
Initial photon number, I0
(counts s-1)		 相干时间Coherence time, τ
(s)		 衰减常量Attenuation
coefficient,
β		 拟合优度Determination
coefficient,
R2
0 7.64 235.07 0.26 0.83 0.993
1 7.93 249.44 0.26 0.85 0.990
2 8.31 281.77 0.29 0.92 0.994
3 8.64 311.75 0.30 0.92 0.993
4 8.73 314.14 0.31 0.93 0.991
5 8.56 307.29 0.31 0.93 0.996
6 8.70 312.99 0.31 0.94 0.992
7 8.61 299.47 0.31 0.93 0.993
8 8.67 318.98 0.32 0.93 0.995

图3

高粱萌发过程中延迟光子辐射积分强度的变化"

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