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作物学报 ›› 2018, Vol. 44 ›› Issue (05): 783-789.doi: 10.3724/SP.J.1006.2018.00783

• 耕作栽培·生理生化 • 上一篇    

高粱种子萌发过程中超微弱光子辐射的特征

赵燕燕1,*(), 习岗2   

  1. 1郑州工业应用技术学院, 河南郑州 451100
    2西安理工大学理学院应用物理系, 陕西西安 710048
  • 收稿日期:2017-09-08 接受日期:2018-01-08 出版日期:2018-05-20 网络出版日期:2018-01-29
  • 通讯作者: 赵燕燕
  • 作者简介:

    第一作者联系方式: E-mail: anjp812@163.com

  • 基金资助:
    本研究由国家自然科学基金项目(31471412, 51277151)资助

Characteristics of Ultra-weak Photon Emission from Sorghum Seeds during Germination

Yan-Yan ZHAO1,*(), Gang XI2   

  1. 1 Zhengzhou University of Industrial Technology, Zhengzhou 451100, Henan, China
    2 Department of Applied Physics, Faculty of Sciences, Xi’an University of Technology, Xi’an 710048, Shaanxi, China;
  • Received:2017-09-08 Accepted:2018-01-08 Published:2018-05-20 Published online:2018-01-29
  • Contact: Yan-Yan ZHAO
  • Supported by:
    This study was supported by the National Natural Science Foundation of China (31471412, 51277151).

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摘要：

采用50 μg mL-1的蛋白质合成转录抑制剂放线菌素D (actinomycin D, AMD)、100 μg mL-1的蛋白质合成翻译抑制剂环己亚胺(cycloheximide, CHM)和650 μg mL-1的呼吸抑制剂NaN3处理萌发高粱种子, 研究了高粱种子萌发过程中自发光子辐射和LED诱导的延迟光子辐射的动力学特征及其变化规律。结果发现, 高粱萌发过程中的自发光子辐射强度与种子鲜质量的变化呈正相关(相关系数r为0.93121), AMD和CHM不同程度地抑制了萌发过程中高粱鲜质量的增长, NaN3几乎完全抑制了鲜质量的增长, 表明高粱萌发过程中的自发光子辐射强度可以作为萌发状况的信号, RNA/DNA合成反应是高粱萌发过程中自发光子辐射的来源之一, 呼吸代谢是其主要来源。高粱萌发过程中的延迟光子辐射特征可用动力学参数初始光子数、相干时间和延迟光子辐射积分强度定量表达, 通过对延迟光子辐射积分强度的测量和分析可以实时、灵敏和无损伤的了解和判断高粱萌发过程中细胞代谢系统的动态变化。

关键词: 高粱, 萌发, 超微弱光子辐射, 蛋白质合成抑制剂, 呼吸代谢抑制剂, 无损检测

Abstract:

Ultra-weak photon radiation from germinated seed includes spontaneous photon emission of germinated seeds in the dark and delayed photon radiation induced by external light. In order to study dynamical feature and biological significance of ultra-weak photon radiation from sorghum during germination, 50 μg mL-1 protein synthesis transcription inhibitor actinomycin D (AMD), 100 μg mL-1 protein synthesis translation inhibitor cycloheximide (CHM) and 650 μg mL-1 respiration inhibitor were adopted to treat germinated sorghum seeds separately. There was a positive correlation (correlation coefficient was 0.93121) between the spontaneous photon emission intensity of germinated sorghum and seed fresh mass. AMD and CHM partially inhibited while NaN3 almost completely inhibited the increase of fresh mass of sorghum seeds in germination process. It indicated that the spontaneous photon emission intensity from germinated sorghum seed can be taken as a signal of germination status from which RNA/DNA synthesis reaction is one of the sources and respiratory metabolism is the main source. The characteristic of delayed photon emission from germinated sorghum seeds can be expressed quantitatively by dynamical parameters such as initial photon number, coherence time and the integrated intensity of delayed photon emission. The cell metabolism and its changes in germination process of sorghum seeds can be understand and evaluated through a real-time, sensitive and non-destructive analysis of the integrated intensity of delayed photon emission from germinated sorghum seeds.

Key words: sorghum, germination, ultra-weak photon radiation, protein synthesis inhibitor, respiratory metabolic inhibitor, non-destructive test

图1

高粱萌发过程中鲜质量的变化"

图2

高粱萌发过程中自发光子辐射的变化"

表1

对照组萌发高粱延迟光子辐射动力学参数"

萌发时间Germination time
(d)		 自发光子辐射Spontaneous photon emission, ISL
(counts s-1)		 初始光子数
Initial photon number, I0
(counts s-1)		 相干时间
Coherence
time, τ
(s)		 衰减常量Attenuation
coefficient,
β		 拟合优度Determination
coefficient,
R2
0 7.64 237.28 0.26 0.84 0.993
1 8.57 256.05 0.27 0.87 0.992
2 9.51 298.72 0.30 0.95 0.996
3 10.38 333.00 0.32 0.96 0.992
4 10.39 337.77 0.35 0.97 0.995
5 10.17 323.95 0.32 0.96 0.991
6 10.41 344.55 0.34 0.97 0.995
7 10.27 331.29 0.33 0.96 0.996
8 10.42 353.76 0.38 0.97 0.996

表2

NaN3处理组萌发高粱延迟光子辐射动力学参数"

萌发时间
Germination time
(d)		 自发光子辐射Spontaneous photon emission, ISL
(counts s-1)		 初始光子数
Initial photon
number, I0
(counts s-1)		 相干时间
Coherence
time, τ
(s)		 衰减常量
Attenuation
coefficient,
β		 拟合优度
Determination
coefficient,
R2
0 7.64 234.28 0.25 0.83 0.992
1 7.78 245.65 0.26 0.84 0.996
2 7.96 269.56 0.27 0.86 0.993
3 8.02 278.51 0.28 0.87 0.991
4 7.56 233.87 0.23 0.81 0.991
5 7.16 228.73 0.22 0.78 0.991
6 7.27 229.76 0.23 0.80 0.994
7 7.16 223.19 0.21 0.80 0.993
8 7.18 226.09 0.22 0.78 0.996

表3

AMD处理组萌发高粱延迟光子辐射动力学参数"

萌发时间Germination time
(d)		 自发光子辐射Spontaneous photon emission, ISL
(counts s-1)		 初始光子数
Initial photon number, I0
(counts s-1)		 相干时间
Coherence
time, τ
(s)		 衰减常量Attenuation
coefficient,
β		 拟合优度Determination
coefficient,
R2
0 7.64 235.44 0.26 0.84 0.994
1 8.17 252.95 0.26 0.86 0.994
2 8.67 290.64 0.29 0.93 0.996
3 9.13 318.03 0.32 0.95 0.992
4 9.15 322.93 0.32 0.95 0.994
5 8.98 305.41 0.32 0.95 0.995
6 9.11 317.80 0.33 0.95 0.994
7 9.06 309.35 0.32 0.94 0.992
8 9.19 324.50 0.34 0.94 0.992

表4

CHM处理组萌发高粱延迟光子辐射动力学参数"

萌发时间Germination time
(d)		 自发光子辐射Spontaneous photon emission, ISL
(counts s-1)		 初始光子数
Initial photon number, I0
(counts s-1)		 相干时间Coherence time, τ
(s)		 衰减常量Attenuation
coefficient,
β		 拟合优度Determination
coefficient,
R2
0 7.64 235.07 0.26 0.83 0.993
1 7.93 249.44 0.26 0.85 0.990
2 8.31 281.77 0.29 0.92 0.994
3 8.64 311.75 0.30 0.92 0.993
4 8.73 314.14 0.31 0.93 0.991
5 8.56 307.29 0.31 0.93 0.996
6 8.70 312.99 0.31 0.94 0.992
7 8.61 299.47 0.31 0.93 0.993
8 8.67 318.98 0.32 0.93 0.995

图3

高粱萌发过程中延迟光子辐射积分强度的变化"

[1] 何磊, 陆兆华, 管博, 赵艳云, 王睿彤, 刘洋. 盐碱胁迫对两种高粱种子萌发及幼苗生长的影响. 西北植物学报, 2012, 32: 362-369
doi: 10.3969/j.issn.1000-4025.2012.02.023
He L, Lu Z H, Guan B, Zhao Y Y, Wang R T, Liu Y.Seed germination and seedling growth of the two sorghums under saline-alkaline stress. Acta Bot Boreali-Occident Sin, 2012, 32: 362-369 (in Chinese with English abstract)
doi: 10.3969/j.issn.1000-4025.2012.02.023
[2] 任根增, 崔江慧, 许凤亭, 乔海宇, 常金华. 不同高粱品系萌发期耐碱性筛选与鉴定. 河北农业大学学报, 2016, 39(1): 9-15
doi: 10.13320/j.cnki.jauh.2016.0002
Ren G Z, Cui J H, Xu F T, Qiao H Y, Chang J H.Screening and identification of sorghum cultivars for alkali tolerance during germination.J Agric Univ Hebei, 2016, 39(1): 9-15 (in Chinese with English abstract)
doi: 10.13320/j.cnki.jauh.2016.0002
[3] Tsuchihashi N, Goto Y.Cultivation of sweet sorghum (Sorghum bicolor(L.) Moench) and determination of its harvest time to make use as the raw material for fermentation practiced during rainy season in the dry land of Indonesia. Plant Prod Sci, 2004, 7: 442-448
[4] Walter Z L, Andrea M.Are we ready to cultivate sweet sorghum as a bioenergy feedstock? A review on field management practices.Biomass Bioenergy, 2012, 40: 1-12
doi: 10.1016/j.biombioe.2012.01.048
[5] 籍贵苏, 杜瑞恒, 刘国庆, 李素英, 侯升林, 吕芃, 马雪, 张之越, 赵全民. 高粱耐盐性评价方法研究及耐盐碱资源的筛选. 植物遗传资源学报, 2013, 14: 25-30
Ji G S, Du R H, Liu G Q, Li S Y, Hou S L, Lyu F, Ma X, Zhang Z Y, Zhao Q M.Methodology and screen of saline-alkaline tolerance in sorghum accessions.J Plant Genet Resour, 2013, 14: 25-30 (in Chinese with English abstract)
[6] Almodares A, Hotjatabady R H, Mirniam E.Effects of drought stress on biomass and carbohydrate contents of two sweet sorghum cultivars.J Environ Biol, 2013, 34: 585-589
doi: 10.1016/Mclepro.2012.10.031 pmid: 24617146
[7] Patane C, Saita A, Sortino O.Comparative effects of salt and water stress on seed germination and early embryo growth in two cultivars of sweet sorghum.J Agron Crop Sci, 2013, 199: 30-37
doi: 10.1111/j.1439-037X.2012.00531.x
[8] 孙璐, 周宇飞, 汪澈, 肖木辑, 陶冶, 许文娟, 黄瑞冬. 高粱品种萌发期耐盐性筛选与鉴定. 中国农业科学, 2012, 45: 1714-1722
doi: 10.3864/j.issn.0578-1752.2012.09.006
Sun L, Zhou Y F, Wang C, Xiao M J, Tao Y, Xu W J, Huang R D.Tolerance during germination screening and identification of sorghum cultivars for salinity.Sci Agric Sin, 2012, 45: 1714-1722 (in Chinese with English abstract)
doi: 10.3864/j.issn.0578-1752.2012.09.006
[9] 王艺陶, 周宇飞, 李丰先, 依兵, 白薇, 闫彤, 许文娟, 高明超, 黄瑞冬. 基于主成分和SOM聚类分析的高粱品种萌发期抗旱性鉴定与分类. 作物学报, 2014, 40: 110-121
doi: 10.3724/SP.J.1006.2014.00110
Wang Y T, Zhou Y F, Li F X, Yi B, Bai W, Yan T, Xu W J, Gao M C, Huang R D.Identification and classification of sorghum cultivars for drought resistance during germination stage based on principal components analysis and self organizing map cluster analysis.Acta Agron Sin, 2014, 40: 110-121 (in Chinese with English abstract)
doi: 10.3724/SP.J.1006.2014.00110
[10] 顾樵. 生物光子学(第2版). 北京: 科学出版社, 2012, p 30
Gu Q.Biophotonics, 2nd edn. Beijing: Science Press, 2012. p 30 (in Chinese)
[11] Rastogi A, Pospísil P.Effect of exogenous hydrogen peroxide on biophoton emission from radish root cells.Plant Physiol Biochem, 2010, 48: 117-123
doi: 10.1016/j.plaphy.2009.12.011 pmid: 20106674
[12] Cifra M, Pospisil P.Ultra-weak photon emission from biological samples: definition, mechanisms, properties, detection and applications.J Photochem Photobiol B, 2014, 139: 2-10
doi: 10.1016/j.jphotobiol.2014.02.009 pmid: 24726298
[13] Kato K, Iyozumi H, Kageyama C, Inagaki H, Yamaguchi A, Nukui H.Application of ultra-weak photon emission measurements in agriculture.J Photochem Photobiol B, 2014, 139: 54-62
doi: 10.1016/j.jphotobiol.2014.06.010 pmid: 25017819
[14] Pospíšil P, Prasad A, Marek R.Role of reactive oxygen species in ultra-weak photon emission in biological systems.J Photochem Photobiol B, 2014, 139: 11-23
doi: 10.1016/j.jphotobiol.2014.02.008 pmid: 24674863
[15] Costanzo E, Gulino M, Lanzanò L, Musumeci F, Scordino A, Tudisco S, Sui L.Single seed viability checked by delayed luminescence.Eur Biophys J Biophy, 2008, 37: 235-238
doi: 10.1007/s00249-007-0221-8 pmid: 17952430
[16] Scordino A, Musumeci F, Gulino M, Lanzanò L, Tudisco S, Sui L, Grasso R, Tviglia A.Delayed luminescence of microalgae as indicator of metal toxicity.J Phys D App Phys, 2008, 41: 155-507
doi: 10.1088/0022-3727/41/15/155507
[17] Rastogi A, Pospíšil P.Ultra-weak photon emission as a non-invasive tool for the measurement of oxidative stress induced by UVA radiation in Arabidopsis thaliana. J Photochem Photobiol B, 2013, 123: 59-64
doi: 10.1016/j.jphotobiol.2013.03.012 pmid: 23624533
[18] Ohya T, Yoshida S, Kawabata R, Okabe H, Kai S.Biophoton emission due to drought injury in red beans: possibility of early detection of drought injury.Jpn J Appl Phys, 2002, 41: 4766-4771
doi: 10.1143/JJAP.41.4766
[19] Yan Y, Popp F A, Rothe G M.Correlation between germination capacity and biophoton emission of barley seeds (Hordeum vulgare L.). Seed Sci Technol, 2003, 31: 249-258
doi: 10.15258/sst.2003.31.2.03
[20] Kobayashi M, Sasaki K, Enomoto M, Ehara Y.Highly sensitive determination of transient generation of biophotons during hypersensitive response to cucumber mosaic virus in cowpea.J Exp Bot, 2007, 58: 465-472
doi: 10.1093/jxb/erl215 pmid: 17158510
[21] 李少华, 习岗, 樊琳琳, 刘锴. 渗透胁迫下萌发小麦种子超弱光子辐射的变化及其意义. 光子学报, 2011, 40: 282-288
doi: 10.3788/gzxb20114002.0282
Li S H, Xi G, Fan L L, Liu K.Changes of ultra-weak photon emission of wheat seed during germination and its significance under osmotic stress,Acta Phot Sin, 2011, 40: 282-288 (in Chinese with English abstract)
doi: 10.3788/gzxb20114002.0282
[22] Komatsu S, Hena A, Kamal M, Makino T, Hossain Z.Ultraweak photon emission and proteomics analyses in soybean under abiotic stress.Biochim Biophys Acta, 2014, 1844: 1208-1218
doi: 10.1016/j.bbapap.2014.04.002 pmid: 24726903
[23] 高宇, 习岗, 刘锴, 杨运经. 基于生物延迟发光评价玉米萌发期抗旱性的方法. 发光学报, 2014, 35: 243-250
doi: 10.3788/fgxb20143502.0243
Gao Y, Xi G, Liu K, Yang Y J.Evaluation method of maize drought resistance during germination based on delayed luminescence.Chin J Lumin, 2014, 35: 243-250 (in Chinese with English abstract)
doi: 10.3788/fgxb20143502.0243
[24] 毛大璋, 沈恂, 张月敬, 李新愿, 戴军, 周佩珍. 代谢抑制剂对萌发绿豆超弱发光的影响. 生物物理学报, 1988, 4: 116-120
Mao D Z, Shen X, Zhang Y J, Li X Y, Dai J, Zhou P Z.Effects of metabolic inhibitors on the ultra-weak photon emission from germinating mung bean seeds.Acta Biophys Sin, 1988, 4: 116-120 (in Chinese with English abstract)
[25] 习岗, 赵燕燕, 刘锴, 贺瑞瑞. 蛋白质合成抑制剂对萌发玉米超弱光子辐射的影响. 农业工程学报, 2015, 31(10): 303-308
doi: 10.11975/j.issn.1002-6819.2015.10.041
Xi G, Zhao Y Y, Liu K, He R R.Effect of protein synthesis inhibitors on ultraweak photon emission during germination of corn.Trans CSAE, 2015, 31(10): 303-308 (in Chinese with English abstract)
doi: 10.11975/j.issn.1002-6819.2015.10.041
[26] 习岗, 傅志东. 外磁场对小麦萌发期过氧化物酶合成的影响及其激活效应. 植物生理学报, 1993, 19: 155-161
Xi G, Fu Z D.Effect of external magnetic field on peroxidase synthesis and its activation during germination in wheat.Acta Phytophysiol Sin, 1993, 19: 155-161 (in Chinese with English abstract)
[27] 孟令波. 玉米种子萌发的蛋白质组学研究. 东北林业大学博士学位论文. 黑龙江哈尔滨, 2008
Meng L B.Proteomic Research during Maize (Zea mays L.) Seed Germination. PhD Dissertation of Northeast Forest University, Harbin, Heilongjiang, China, 2008 (in Chinese with English abstract)
[28] Inomata K, Tanaka H.Protective effect of benidipine against sodium azide-induced cell death in cultured neonatal rat cardiac myocytes.J Pharmacol Sci, 2003, 93: 163-170
doi: 10.1254/jphs.93.163 pmid: 14578584
[29] Perez F J, Vergara R, Or E.On the mechanism of dormancy release in grapevine buds: a comparative study between hydrogen cyanamide and sodium azide.Plant Growth Regul, 2009, 59: 145-152
doi: 10.1007/s10725-009-9397-5
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