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作物学报 ›› 2019, Vol. 45 ›› Issue (3): 477-482.doi: 10.3724/SP.J.1006.2019.84035

• 研究简报 • 上一篇    

雪茄烟品种Beinhart1000-1赤星病抗性基因的QTL定位

童治军,张谊寒,陈学军,曾建敏,方敦煌(),肖炳光()   

  1. 云南省烟草农业科学研究院 / 烟草行业烟草生物技术育种重点实验室 / 国家烟草基因工程研究中心, 云南昆明 650021
  • 收稿日期:2018-03-05 接受日期:2018-12-24 出版日期:2019-03-12 网络出版日期:2019-01-05
  • 通讯作者: 方敦煌,肖炳光
  • 作者简介:E-mail: tzj861@163.com
  • 基金资助:
    本研究由云南省基础研究计划项目(2018FB064);中国烟草总公司项目(110201601027(JY-01));中国烟草总公司项目(110201603008);中国烟草总公司云南省公司项目(2017YN01);中国烟草总公司云南省公司项目资助(2016YN23)

Mapping of quantitative trait loci conferring resistance to brown spot in cigar tobacco cultivar Beinhart1000-1

Zhi-Jun TONG,Yi-Han ZHANG,Xue-Jun CHEN,Jian-Min ZENG,Dun-Huang FANG(),Bing-Guang XIAO()   

  1. Yunnan Academy of Tobacco Agricultural Sciences / Key Laboratory of Tobacco Biotechnological Breeding / National Tobacco Genetic Engineering Research Center, Kunming 650021, Yunnan, China
  • Received:2018-03-05 Accepted:2018-12-24 Published:2019-03-12 Published online:2019-01-05
  • Contact: Dun-Huang FANG,Bing-Guang XIAO
  • Supported by:
    This study was supported by the Fundamental Research Program of Yunnan Province(2018FB064);China National Tobacco Company(110201601027(JY-01));China National Tobacco Company(110201603008);Yunnan Tobacco Company(2017YN01);Yunnan Tobacco Company(2016YN23)

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摘要：

由链格孢菌(Alternaria alternata)引起的赤星病是最具破坏性的烟草叶斑病害之一, 在中国严重地影响烟草(Nicotiana tabacum L.)的产量和质量。选育抗烟草赤星病的优良品种虽是预防该病最经济、有效的途径, 但因其抗性受数量性状基因控制而很难通过常规育种手段实现。为了便于开展烟草抗赤星病分子标记辅助选择育种, 本研究利用抗赤星病雪茄烟品种Beinhart1000-1和感病烤烟品种红花大金元经杂交、自交产生的362个F2单株构建了一张包含670个SSR标记的烟草遗传连锁图谱, 并结合组培快繁形成的F2株系的田间赤星病病情指数(DI), 在全基因组范围内检测获得2个与烟草赤星病抗性相关的QTL, 分别位于第20和23连锁群上的SSR标记TMs05179和TMs04022, 以及TM61049和TM62212之间。这2个QTL等位基因均来自抗病亲本Beinhart1000-1, 它们一起解释了两亲本间81%的病情指数(DI)差异及64%的加性效应。为下一步开展烟草抗赤星病的分子标记辅助育种奠定了基础。

关键词: 烟草赤星病, 简单重复序列, 遗传连锁图谱, 数量性状基因座

Abstract:

Tobacco brown spot (TBS) caused by Alternaria alternata is one of the most destructive foliar diseases affecting tobacco (Nicotiana tabacum L.) production and quality in China. Breeding TBS-resistant cultivars is difficult by traditional method because the resistance has proved to be quantitatively inherited. To facilitate marker-assisted selection, we carried out a study of mapping quantitative trait loci (QTLs) for TBS resistance. We developed an F2 population consisting of 362 individuals from a cross between a TBS-susceptive flue-cured tobacco Honghua Dajinyuan (HD) and a TBS-resistant cigar tobacco cultivar Beinhart1000-1, and constructed a genetic map consisting of 670 SSR markers based on this population. Using disease index (DI) as the indicator of TBS resistance, we detected two QTLs located between SSR markers TMs05179 and TMs04022, and TM61049 and TM62212 on linkage group (LG) 20 and LG23, respectively. The resistant alleles of the two QTLs were all from the resistant parent Beinhart1000-1. The two QTLs together could explain 81% of the DI difference between the two parents in total, and 64% of their additive effects. Therefore, the two QTLs will be useful for TBS resistance breeding.

Key words: tobacco brown spot (TBS), simple sequence repeats (SSR), genetic linkage map, quantitative trait locus (QTL)

图1

烟草赤星病病情指数在F2群体中的分布频率"

图2

基于362个F2单株(红花大金元×Beinhart1000-1)的烟草赤星病抗性QTL定位结果 图中每条连锁群的左右两侧分别为遗传距离(厘摩尔)和标记名称, 其中, 红色标记表示位于Bindler等[30,31]公布的连锁群上。"

表1

烟草赤星病抗性QTL定位结果"

QTL LG 位置
Position		 两侧标记
Marker interval		 LOD 加性效应a
Additive a		 显性效应
Dominant		 贡献率b
Exp b(%)
qTBS20 LG20 62.414 TMs05179-TMs04022 5.82 -15.874 -1.819 18.31
qTBS23 LG23 79.091 TM61049-TM62212 3.51 -10.923 11.145 8.94
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