作物学报 ›› 2019, Vol. 45 ›› Issue (12): 1796-1805.doi: 10.3724/SP.J.1006.2019.91025
伦珠朗杰1,2,3,4,5,李慧慧5,郭刚刚5,其美旺姆2,3,高丽云2,3,唐亚伟2,3,尼玛扎西2,4,达瓦顿珠2,3,*(),卓嘎1,*()
Lhundrupnamgyal1,2,3,4,5,Hui-Hui LI5,Gang-Gang GUO5, Chemiwangmo2,3,Li-Yun GAO2,3,Ya-Wei TANG2,3, Nyematashi2,4, Dawadondrup2,3,*(), Dolkar1,*()
摘要:
为提高青稞种质资源的利用效率并筛选优异杂交亲本, 分别在西藏林芝和拉萨两地的春播和秋播环境下对1605份青稞种质资源进行冬春性鉴定和抽穗期多样性分析。设拉萨春播2个播期, 即正常春播I和晚10 d春播II, 以2个播期的抽穗期变化作为冬春性区别依据。结果表明, 96.2%的西藏青稞地方品种为春性, 在西藏3个生态区均有分布, 冬性品种仅有3.8%, 且主要分布在以林芝为主的藏东南生态区; 抽穗期的Shannon-Wiener’s多样性分析表明, 春播条件下抽穗期多样性高于秋播, 拉萨春播条件下抽穗期多样性最高, 林芝春播次之, 林芝秋播最低; 在相同环境下, 地方品种的多样性高于育成品种; 在环境稳定性分析中, 368份春性地方品种和21份育成品种在不同环境中抽穗期较为稳定, 其中康青3号在两地所有参试品种中抽穗期稳定性最高。本研究为全面理解西藏青稞资源的冬春性、抽穗期多样性和环境稳定性提供了参考, 为广适应性青稞品种培育筛选出环境稳定性佳的亲本材料。
[1] |
Pourkheirandish M, Komatsuda T . The importance of barley genetics and domestication in a global perspective. Ann Bot, 2007,100:999-1008.
doi: 10.1093/aob/mcm139 |
[2] |
Turner A, Beales J, Faure S, Dunford R P, Laurie D A . The pseudo-response regulator Ppd-H1 provides adaptation to photoperiod in barley. Science, 2005,310:1031-1034.
doi: 10.1126/science.1117619 |
[3] |
Jones H, Leigh F J, Mackay I, Bower M A, Smith L M, Charles M P, Jones G, Jones M K, Brown T A, Powell W . Population based resequencing reveals that the flowering time adaptation of cultivated barley originated east of the Fertile Crescent. Mol Biol Evol, 2008,25:2211-2219.
doi: 10.1093/molbev/msn167 |
[4] |
Ibrahim A, Harrison M, Meinke H, Fan Y, Johnson P, Zhou M . A regulator of early flowering in barley (Hordeum vulgare L.). PLoS One, 2018,13:e0200722.
doi: 10.1371/journal.pone.0200722 |
[5] |
Doebley J F, Gaut B S, Smith B D . The molecular genetics of crop domestication. Cell, 2006,127:1309-1321.
doi: 10.1016/j.cell.2006.12.006 |
[6] |
Sang T . Genes and mutations underlying domestication transitions in grasses. Plant Physiol, 2009,149:63-70.
doi: 10.1104/pp.108.128827 |
[7] |
Trevaskis B, Bagnall D J, Ellis M H, Peacock W J, Dennis E S . MADS box genes control vernalization-induced flowering in cereals. Proc Natl Acad Sci USA, 2003,100:13099-13104.
doi: 10.1073/pnas.1635053100 |
[8] |
Saisho D, Ishii M, Hori K, Sato K . Natural variation of barley vernalization requirements: implication of quantitative variation of winter growth habit as an adaptive trait in East Asia. Plant Cell Physiol, 2011,52:775-784.
doi: 10.1093/pcp/pcr046 |
[9] |
Nishida H, Yoshida T, Kawakami K, Fujita M, Long B, Akashi Y, Laurie D A, Kato K . Structural variation in the 5′ upstream region of photoperiod-insensitive alleles Ppd-A1a and Ppd-B1a identified in hexaploid wheat(Triticum aestivum L.), and their effect on heading time. Mol Breed, 2012,31:27-37.
doi: 10.1007/s11032-012-9765-0 |
[10] |
Comadran J, Kilian B, Russell J, Ramsay L, Stein N, Ganal M, Shaw P, Bayer M, Thomas W, Marshall D, Hedley P, Tondelli A, Pecchioni N, Francia E, Korzun V, Walther A, Waugh R . Natural variation in a homolog of Antirrhinum CENTRORADIALIS contributed to spring growth habit and environmental adaptation in cultivated barley. Nat Genet, 2012,44:1388-1392.
doi: 10.1038/ng.2447 |
[11] |
Laurie D A, Pratchett N, Bezant J H, Snape J W . RFLP mapping of five major genes and eight quantitative trait loci controlling flowering time in a winter × spring barley (Hordeum vulgare L.) cross. Genome, 1995,38:575-585.
doi: 10.1139/g95-074 |
[12] |
Yan L L, Loukoianov A, Tranquilli G, Helguera M, Fahima T, Dubcovsky J . Positional cloning of the wheat vernalization gene VRN1. Proc Natl Acad Sci USA, 2003,100:6263-6268.
doi: 10.1073/pnas.0937399100 |
[13] |
Yan L L, Loukoianov A, Blechl A, Tranquilli G, Ramakrishna W, Sanmiguel P, Bennetzen J L, Echenique V, Dubcovsky J . The wheat VRN2 gene is a flowering repressor down-regulated by vernalization. Science, 2004,303:1640-1644.
doi: 10.1126/science.1094305 |
[14] |
Yan L L, Fu D, Li C, Blechl A, Tranquilli G, Bonafede M, Sanchez A, Valarik M, Yasuda S, Dubcovsky J . The wheat and barley vernalization gene VRN3 is an orthologue of FT. Proc Natl Acad Sci USA, 2006,103:19581-19586.
doi: 10.1073/pnas.0607142103 |
[15] |
Faure S, Higgins J, Turner A, Laurie D A . The FLOWERING LOCUS T-like gene family in barley (Hordeum vulgare). Genetics, 2007,176:599-609.
doi: 10.1534/genetics.106.069500 |
[16] |
Distelfeld A, Li C, Dubcovsky J . Regulation of flowering in temperate cereals. Curr Opin Plant Biol, 2009,12:178-184.
doi: 10.1016/j.pbi.2008.12.010 |
[17] | 卢良恕 . 中国大麦学. 北京: 中国农业出版社, 1996. |
Lu L S. Chinese Barley. Beijing: China Science and Technology Press, 1996. pp 3-7(in Chinese). | |
[18] | 胡颂杰 . 西藏农业概论. 成都: 四川科学技术出版社, 1995. |
Hu S J. An Introduction of Tibet Agriculture. Chengdu: Sichuan Science and Technology Press, 1995 ( in Chinese) | |
[19] | Tashi N. Food preparation from hull-less barley in Tibet. In: Food Barley—Importance, Uses and Local Knowledge: Proc. International Workshop on Food Barley Improvement, 2002. Grando S, Macpherson H G, eds. ICARDA, Aleppo, Syria, 2005. |
[20] | 朱睦元, 张京 . 大麦(青稞)营养分析及其食品加工. 杭州: 浙江大学出版社, 2015. |
Zhu M Y, Zhang J. Barley Nutritional Analysis and Food Processing. Hangzhou: Zhejiang University Press, 2015 (in Chinese). | |
[21] | 马得泉 . 中国西藏大麦遗传资源. 北京: 中国农业出版社, 2000. |
Ma D Q. Genetic Resources of Tibetan Barley in China. Beijing: China Science and Technology Press, 2000 (in Chinese). | |
[22] | 张京, 曹永生 . 我国大麦基因库的群体结构和表型多样性研究. 中国农业科学, 1998,32(4):20-26. |
Zhang J, Cao Y S . Population Structure and Phenotypic Diversity of Barley Gene Bank in China. Sci Agric Sin, 1998 (in Chinese with English abstract). | |
[23] | 冯宗云 . 徐廷文大麦学术文集. 成都四川科学技术出版社, 2006. |
Feng Z Y. Xu Tingwen's Work for Barley Science. Chengdu: Sichuan Science and Technology Press, 2006 ( in Chinese) | |
[24] | 达瓦顿珠. 中国大麦低温春化和光周期基因单倍型及表型关联分析. 中国农业科学院研究生院博士学位论文, 北京, 2015. pp 25-28. |
Dawadondrop. Haplotypes and Phenotypic Association Analsis of Vernalization and Photoperiod Genes in Chinese Barley in china. PhD Dissertation of Chinese Academy of Agricultural Sciences, Beijing, China, 2015. pp 25-28 (in Chinese with English abstract). | |
[25] |
Alqudah A M, Sharma R, Pasam R K, Graner A, Kilian B, Schnurbusch T . Genetic dissection of photoperiod response based on GWAS of pre-anthesis phase duration in spring barley. PLoS One, 2014,9:e113120.
doi: 10.1371/journal.pone.0113120 |
[26] |
Alqudah A M, Schnurbusch T . Heading date is not flowering time in spring barley. Front Plant Sci, 2017,8:896.
doi: 10.3389/fpls.2017.00896 |
[27] |
Hemming M N, Fieg S, Peacock W J, Dennis E S, Trevaskis B . Regions associated with repression of the barley (Hordeum vulgare) VERNALIZATION1 gene are not required for cold induction. Mol Genet Genom, 2009,282:107-117.
doi: 10.1007/s00438-009-0449-3 |
[28] |
Dondup D, Dong G Q, Xu D D, Zhang L S, Zha S, Yuan X M, Tashi N, Zhang J, Guo G G . Allelic variation and geographic distribution of vernalization genes HvVRN1 and HvVRN2 in Chinese barley germplasm. Mol Breed, 2016,36:11
doi: 10.1007/s11032-016-0434-6 |
[29] | Shannon C E, Weaver W. The Mathematical Theory of Communication. Urbana: University of Illinons Press, 1949. |
[30] | 王建康 . 数量遗传学. 北京: 科学出版社, 2017. |
Wang J K. Quantitative Genetics. Beijing: Science Press, 2017 (in Chinese). | |
[31] | 强小林 . 西藏青稞品种系谱及其分析. 中国作物学会大麦专业委员会. 中国大麦文集. 北京: 中国农业科技出版社, 1986. pp 189-195. |
Qiang X L. The pedigree and its analysis of barley varieties in Tibet. In: Barley Professional Committee of China Crop Society. Barley Science in China. Beijing: China Agricultural Science and Technology Press, 1986. pp 189-195(in Chinese). |
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