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作物学报 ›› 2020, Vol. 46 ›› Issue (01): 140-146.doi: 10.3724/SP.J.1006.2020.92022

• 研究简报 • 上一篇    下一篇

增加穗粒数的水稻染色体代换系Z747鉴定及相关性状QTL定位

王大川,汪会,马福盈,杜婕,张佳宇,徐光益,何光华,李云峰,凌英华,赵芳明()   

  1. 西南大学水稻研究所/西南大学农业科学研究院, 重庆 400715
  • 收稿日期:2019-04-15 接受日期:2019-08-09 出版日期:2020-01-12 网络出版日期:2019-09-11
  • 通讯作者: 赵芳明
  • 作者简介:E-mail: wangdachuan6@163.com
  • 基金资助:
    本研究由国家重点研发计划项目(2017YFD0100202);重庆市科委主题专项(CSTC, 2016shms-ztzx0017);西南大学基本业务费专项创新团队项目资助(XDJK2017A004)

Identification of rice chromosome segment substitution Line Z747 with increased grain number and QTL mapping for related traits

WANG Da-Chuan,WANG Hui,MA Fu-Ying,DU Jie,ZHANG Jia-Yu,XU Guang-Yi,HE Guang-Hua,LI Yun-Feng,LING Ying-Hua,ZHAO Fang-Ming()   

  1. Rice Research Institute, Southwest University/Academy of Agricultural Sciences, Southwest University, Chongqing 400715, China
  • Received:2019-04-15 Accepted:2019-08-09 Published:2020-01-12 Published online:2019-09-11
  • Contact: Fang-Ming ZHAO
  • Supported by:
    This study was supported by the National Key Research and Development Program of China(2017YFD0100202);Project of Chongqing Science & Technology Commission(CSTC, 2016shms-ztzx0017);Fundamental Research Funds for the Central Universities(XDJK2017A004)

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摘要：

增加穗粒数对水稻高产品种培育至关重要。其遗传基础复杂, 由多基因控制。水稻染色体片段代换系可以将多基因控制的复杂性状分解, 因而是理想的遗传研究材料。本研究通过高代回交和自交结合分子标记辅助选择方法, 鉴定了一个以日本晴为受体、西恢18为供体亲本的、含有15个代换片段的增加穗粒数的水稻染色体片段代换系Z747, 平均代换长度为4.49 Mb。与受体日本晴相比, Z747的每穗总粒数、一次枝梗数、二次枝梗数、穗长和粒长显著增加, 粒宽显著变窄、结实率显著降低, 但结实率仍为81%。进一步以日本晴和Z747杂交构建的次级F2群体鉴定出46个相关性状的QTL, 分布于水稻1号、2号、3号、5号、6号、9号、11号和12号染色体上。其中qGPP12、qPH-3-1、qPH-3-2等12个QTL可能与已克隆的基因等位, qSPP9等34个可能是新鉴定的QTL。Z747的每穗总粒数由2个具有增加粒数效应的QTL (qSPP3和qSPP5)和1个具有减少粒数效应的QTL (qSPP9)控制。研究结果对主效QTL的精细定位和克隆、以及有利基因的单片段代换系培育有重要意义。

关键词: 水稻, 染色体片段代换系, 粒数, QTL定位

Abstract:

Increasing grain number per panicle is important for rice breeding for high yield. Its inheritance is very complex and controlled by many genes. Chromosome segment substitution lines can dissect complex traits controlled by many genes, and thus are ideal genetic research materials. Here, an excellent rice chromosome segment substitution line Z747 with increased grain number was identified from recipient Nipponbare and donor Xihui 18 through advanced backcrossing and inbreeding combined SSR marker-assisted selection. Z747 carried fifteen substitution segments with 4.49 Mb of average length. Compared with Nipponbare, Z747 had significantly increased spikelet number per panicle, number of primary branches, number of secondary branches, panicle length and grain length, and decreased grain width and seed setting rate. However, the seed setting rate in Z747 was still up to 81%. Furthermore, secondary F2 population from crosses between Nipponbare and Z747 was used to map QTL for related traits. A total of 46 QTLs distributed on chromosomes 1, 2, 3, 5, 6, 9, 11, and 12 were detected. Among them, 12 QTLs such as qGPP12, qPH-3-1, and qPH-3-2 etc. might be alleles of cloned genes, and the remaining 34 QTLs such as qSPP9 etc. might not be identified in the past. The spikelet number per panicle of Z747 was mainly controlled by two QTLs (qSPP3 and qSPP5) with effects of increasing spikelet number and one (qSPP9) with decreasing effects. These results are important for fine mapping and cloning of major QTL, and developing single-segment substitution lines carrying favorable QTLs.

Key words: rice, chromosome segment substitution lines, grain number, QTL mapping

图1

Z747的代换片段及携带的QTL 每条染色体左侧为物理距离(Mb)、定位的QTL; 右侧为标记名称、代换区间(框内标记)和代换长度(黑箭头指向)。PH: 株高; PL: 穗长; NPB: 一次枝梗数; NSB: 二次枝梗数; GPP: 每穗实粒数; SPP: 每穗总粒数; SSR: 结实率; GL: 粒长; GW: 粒宽; RLW: 谷粒长宽比; GWT: 千粒重。部分有上标[20]~[30]的QTL表示可能与已克隆的基因等位。[20]: OsRLCK102; [21]: HTD2; [22]: pbr2; [23]: JMJ703; [24]: ND1; [25]: DFO1; [26]: OsMCA1; [27]: OsFRDL1; [28]: PTB1; [29]: DCM1; [30]: OsVPE3。"

图2

日本晴和Z747的表型"

表1

日本晴和Z747及F2群体各性状统计参数"

性状
Trait		 平均值±标准差(亲本)
Mean±SD (parents)		 F2群体
F2 population
日本晴
Nipponbare		 Z747 平均值±标准差
Mean±SD		 范围
Range		 偏度
Skewness		 峰度
Kurtosis
株高 Plant height (cm) 88.1±3.4 91.8±6.9 91.9±8.2 68.0-130.8 0.39 0.31
穗长Panicle length (cm) 19.7±1.2 23.3±1.3** 19.3±1.6 14.8-27.3 -0.18 0.57
有效穗数Panicle number 19.3±5.1 15.5±6.4 15.6±5.5 3.0-36.0 0.40 -0.03
一次枝梗数Number of primary branch 7.4±0.5 12.2±1.3** 8.5±1.1 6.9-13.4 -0.15 0.27
二次枝梗数Number of secondary branch 14.3±1.7 40.5±1.2** 15.1±4.3 7.4-45.6 0.03 0.71
每穗总粒数Spikelet number per panicle 110.7±16.4 173.0±49.6** 94.3±19.9 21.9-221.7 -0.19 0.39
每穗实粒数Grain number per panicle 101.4±16.5 139.8±41.2 79.4±15.6 10.1-142.5 -0.23 0.65
结实率Seed setting rate (%) 91.0±2.0 81.0±0.7** 85.1±12.9 10.9-96.3 -2.17 10.44
粒长Grain length (mm) 7.17±0.24 7.93±0.04** 7.45±0.47 6.61-10.05 0.36 1.81
粒宽Grain width (mm) 3.40±0.08 3.23±0.05* 3.34±0.13 2.38-3.52 -0.38 0.26
千粒重1000-grain weight (g) 24.6±1.6 25.5±0.7 25.5±1.8 21.0-32.3 0.28 1.27
单株产量Yield per plant (g) 38.6±9.8 40.9±5.3 31.7±12.4 2.4-72.5 0.45 0.20

表2

Z747携带的水稻重要农艺性状QTL"

性状
Trait		 QTL 染色体
Chr.		 连锁标记
Linked marker		 加性效应
Additive effect		 贡献率
Var. (%)		 P值
P-value		 可能的等位基因
Possible alleles
株高 qPH-3-1 3 RM5474 -7.79 9.45 0.0004 OsRLCK102[20]
Plant height qPH-3-2 3 RM3417 5.74 5.15 0.0003 HTD2 [21]
qPH-3-3 3 RM6266 5.03 10.30 <0.0001 —
qPH5 5 RM5874 13.67 27.54 0.0003 —
qPH11 11 RM7120 5.31 4.39 0.0090 —
穗长 qPL3 3 RM6266 0.51 2.70 0.0020 —
Panicle length qPL11 11 RM7120 1.28 6.61 0.0012 —
一次枝梗数 qNPB2 2 RM1385 0.59 6.07 0.0002 Pbr2[22]
Number of primary branch qNPB3 3 RM3766 0.83 7.45 0.0012 —
qNPB9 9 RM24537 1.79 9.96 <0.0001 —
qNPB12 12 RM247 -1.07 8.14 0.0049 —
二次枝梗数 qNSB2 2 RM6378 2.33 1.65 0.0004 —
Number of secondary branch qNSB3 3 RM3766 3.86 7.83 0.0005 —
qNSB5 5 RM18067 3.59 5.92 0.0036 JMJ703[23]
qNSB6 6 RM494 -2.46 7.10 0.0004 —
qNSB-9-1 9 RM7048 -7.39 20.71 0.0007 —
qNSB-9-2 9 RM24537 6.29 6.05 0.0001 —
实粒数 qGPP2 2 RM1385 8.89 4.53 0.0014 —
Grain number per panicle qGPP-9-1 9 RM7048 -28.08 19.83 0.0008 —
qGPP-9-2 9 RM24537 18.49 3.66 0.0038 —
qGPP12 12 RM3331 -18.64 8.21 <0.0001 ND1[24]
总粒数 qSPP3 3 RM3766 20.73 9.66 0.0002 —
Spikelet number per panicle qSPP5 5 RM18067 20.07 7.90 0.0015 —
qSPP9 9 RM7048 -29.51 14.15 0.0044 —
结实率 qSSR1 1 RM259 -24.38 11.97 0.0001 DFO1[25]
Seed setting rate qSSR2 2 RM1385 4.65 1.19 0.0069 —
qSSR-3-1 3 RM5474 18.66 9.82 <0.0001 OsMCA1[26]
qSSR-3-2 3 RM3766 -10.38 3.58 0.0014 OsFRDL1[27]
qSSR5 5 RM5874 42.72 46.67 <0.0001 PTB1[28]
qSSR6 6 RM5371 -17.59 6.48 0.0013 DCM1[29]
qSSR-9-1 9 RM7048 -20.74 10.33 0.0004 —
qSSR-9-2 9 RM24537 23.28 5.24 <0.0001 —
qSSR12 12 RM3331 -14.58 4.80 <0.0001 —
粒长 qGL3 3 RM6266 0.40 63.73 <0.0001 —
Grain length qGL5 5 RM18067 0.24 9.29 0.0007 —
qGL12 12 RM3331 0.16 3.21 0.0047 —
粒宽 qGW2 2 RM1385 -0.07 5.59 0.0080 OsVPE3[30]
Grain width qGW5 5 RM18148 -0.08 6.05 0.0029 —
长宽比 qRLW2 2 RM1385 0.08 5.35 0.0004 —
Rate of length to width qRLW-3-1 3 RM3766 0.10 5.42 0.0047 —
qRLW-3-2 3 RM6266 0.13 19.74 <0.0001 —
qRLW5 5 RM18148 0.13 10.17 0.0002 —
qRLW6 6 RM494 -0.09 10.31 <0.0001 —
qRLW12 12 RM3331 0.15 7.70 <0.0001 —
千粒重 qGWT2 2 RM1385 -0.80 4.01 0.0048 OsVPE3[30]
1000-kernel weight qGWT3 3 RM6266 1.82 28.67 <0.0001 —
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