优良水稻染色体片段代换系Z746的鉴定及重要农艺性状QTL定位及其验证

doi:10.3724/SP.J.1006.2021.92002

作物学报 ›› 2021, Vol. 47 ›› Issue (3): 451-461.doi: 10.3724/SP.J.1006.2021.92002

• 作物遗传育种·种质资源·分子遗传学 • 上一篇下一篇

优良水稻染色体片段代换系Z746的鉴定及重要农艺性状QTL定位及其验证

沈文强(), 赵冰冰(), 于国玲, 李凤菲, 朱小燕, 马福盈, 李云峰, 何光华, 赵芳明^*()

西南大学水稻研究所 / 西南大学农业科学研究院 / 转基因植物与安全控制重庆市重点实验室, 重庆 400715

收稿日期:2019-01-09 接受日期:2020-10-14 出版日期:2021-03-12 网络出版日期:2020-11-02
通讯作者: 赵芳明
作者简介:沈文强, E-mail: 412588538@qq.com|赵冰冰, E-mail: 977276044@qq.com
基金资助:
国家重点研发计划项目(2017YFD0100202)(2017YFD0100202);重庆市科委项目(cstc2016shms-ztzx0017);重庆市科委项目(CSTCCXLJRC201713);西南大学基本业务费专项创新团队项目资助(XDJK2017A004)

Identification of an excellent rice chromosome segment substitution line Z746 and QTL mapping and verification of important agronomic traits

SHEN Wen-Qiang(), ZHAO Bing-Bing(), YU Guo-Ling, LI Feng-Fei, ZHU Xiao-Yan, MA Fu-Ying, LI Yun-Feng, HE Guang-Hua, ZHAO Fang-Ming^*()

Rice Research Institute, Southwest University / Academy of Agricultural Sciences, Southwest University / Transgenic Plants and Safety Control, Chongqing Key Laboratory, Chongqing 400715, China

Received:2019-01-09 Accepted:2020-10-14 Published:2021-03-12 Published online:2020-11-02
Contact: ZHAO Fang-Ming
Supported by:
National Key Research and Development Program of China(2017YFD0100202);Project of Chongqing Science & Technology Commission Grants(cstc2016shms-ztzx0017);Project of Chongqing Science & Technology Commission Grants(CSTCCXLJRC201713);Southwest University Innovation Team Project(XDJK2017A004)

摘要/Abstract

摘要：

粒型、株高及穗部组成性状与产量形成密切相关, 是水稻重要农艺性状, 但遗传基础复杂。染色体片段代换系是用于复杂性状遗传研究的良好材料。本研究鉴定了一个以日本晴为受体、西恢18为供体亲本的水稻优良染色体片段代换系Z746。Z746携带来自西恢18的7个代换片段, 平均代换长度为3.99 Mb, 其株高、粒长和穗部性状均与受体存在显著差异。进一步通过日本晴与Z746杂交构建的次级F₂群体共检测到36个相关QTL, 分布于2号、3号、4号、6号和11号染色体。其中5个可能与已克隆基因等位, 如qPH3-1等, 8个可被多次检出, 表明这些是遗传稳定的主效QTL。Z746的粒长主要由4个QTL (qGL3、qGL4、qGL2、qGL6)控制, 其中qGL3和qGL4对粒长变异的贡献率分别为60.28%和27.47%。株高由5个QTL控制, 穗长由4个QTL控制, 每穗粒数由2个QTL控制, 千粒重由2个QTL控制。然后以MAS在F₃共选出8个单片段代换系, 并以此在F₄进行了相关QTL验证, 共有24个QTL可被8个单片段代换系(SSSL)检出, 重复检出率为66.7%, 表明这些QTL遗传稳定。本研究为目的QTL的进一步遗传机制研究及分子设计育种奠定了良好基础。

关键词: 水稻, 染色体片段代换系, 粒长, 株高, 穗部组成, QTL定位

Abstract:

The important agronomic traits, such as grain type, plant height, and panicle composition, are closely related to rice yield, and their gene inheritance is complex. Chromosome segment substitution lines (CSSLs) are useful materials for studying these complex traits. An excellent CSSL, Z746, containing seven substitution segments and with an average substitution length of 3.99 Mb, was identified from Nipponbare as a recipient and ‘Xihui 18’ as a donor parent. There were significant differences in plant height, panicle composition and grain size between Z746 and Nipponbare. Furthermore, a total of 36 quantitative trait loci (QTL) were detected on chromosomes 2, 3, 4, 6, and 11 in the secondary F₂ population constructed by hybridization between Nipponbare and Z746. Five of them may be alleles of the cloned genes, such as qPH3-1, and eight can be detected by multiple times, indicating that these were genetically stable major QTLs. The grain length in Z746 was mainly controlled by four QTLs (qGL3, qGL4, qGL2, and qGL6), and the phenotypic variation of qGL3 and qGL4 for grain length was 60.28% and 27.47%, respectively. Plant height was controlled by five QTLs, panicle length by four QTLs, grain number per panicle by two QTLs, and the 1000-grain weight by two QTLs. Eight single segment substitution lines (SSSLs) were developed in F₃ population by molecular marker-assisted selection (MAS), and relevant QTLs verification was conducted in F₄. Finally, 24 QTLs were detected by 8 SSSLs and the repeat detection rate was 66.7%, indicating that these QTLs were genetically stable. These results provide a good foundation for further research on genetic mechanisms of the target QTLs and molecular design breeding.

Key words: rice (Oryza sativa L.), chromosome segment substitution line, grain length, plant height, spike composition, QTL mapping

沈文强, 赵冰冰, 于国玲, 李凤菲, 朱小燕, 马福盈, 李云峰, 何光华, 赵芳明. 优良水稻染色体片段代换系Z746的鉴定及重要农艺性状QTL定位及其验证[J]. 作物学报, 2021, 47(3): 451-461.

SHEN Wen-Qiang, ZHAO Bing-Bing, YU Guo-Ling, LI Feng-Fei, ZHU Xiao-Yan, MA Fu-Ying, LI Yun-Feng, HE Guang-Hua, ZHAO Fang-Ming. Identification of an excellent rice chromosome segment substitution line Z746 and QTL mapping and verification of important agronomic traits[J]. Acta Agronomica Sinica, 2021, 47(3): 451-461.

图/表 5

图1

图2

表1

表2

表3

SSSL中QTL的加性效应分析"

SSSL代码 SSSL code	代换片段 ^b Substitution segment ^b	长度 Length (Mb)	染色体 Chr.	性状 Trait	QTL	均值±标准差 Mean±SD	加性效应 Additive	P值 P-value
N ^a		—		株高 Plant height (cm)		90.60±4.50
S1	RM2770--RM6378--RM1075	1.04	2		qPH2-1	101.06±1.65	5.23	2.86E-06
S2	RM6378--RM1075--RM5699	2.58	2		qPH2-2	128.12±3.46	18.76	2.58E-13
S3	RM132--RM14389--RM175	7.34	3		qPH3-1	101.15±1.54	5.28	2.31E-06
S4	RM5928--RM6266--RM157	1.43	3		qPH3-2	110.65±3.48	10.02	4.55E-08
S5	RM5688--RM1155--RM17078	2.95	4		qPH4	108.66±3.19	9.03	1.40E-08
S6	RM7412--RM103--RM494	0.90	6		qPH6-1	109.31±2.00	9.36	1.35E-09
S7	RM103--RM494--long arm end	0.20	6		qPH6-2	104.95±1.73	9.36	4.67E-08
S8	RM5371--RM7412-RM103-RM494--long arm end	2.57	6		qPH6	106.20±2.29	7.80	2.93E-08
N ^a		—		穗长 Panicle length (cm)		19.02±1.11
S1	RM2770--RM6378--RM1075	1.04	2		qPL2	22.70±1.29	1.84	8.53E-07
S4	RM5928--RM6266--RM157	1.43	3		qPL3	24.60±2.19	2.79	1.69E-05
S5	RM5688--RM1155--RM17078	2.95	4		qPL4	21.64±1.62	1.31	0.001
S7	RM103--RM494--long arm end	0.20	6		qPL6	26.02±1.92	3.50	4.63E-08
N ^a		—		一次枝梗数 Number of primary branch		9.47±0.55
S2	RM6378--RM1075--RM5699	2.58	2		qNPB2	12.80±1.60	1.67	1.57E-05
S8	RM5371--RM7412-RM103-RM494--long arm end	2.57	6		qNPB6	10.27±0.96	0.40	0.02
N ^a		—		二次枝梗数 Number of secondary branch		16.03±2.54
S1	RM2770--RM6378--RM1075	1.04	2		qNSB2-1	18.82±2.75	1.40	0.03
S2	RM6378--RM1075--RM5699	2.58	2		qNSB2-2	25.78±2.97	4.88	1.38E-06
N ^a		—		粒长 Grain length (mm)		7.25±0.13
S4	RM5928--RM6266--RM157	1.43	3		qGL3	7.71±0.23	0.23	0.0002
S7	RM103--RM494--long arm end	0.20	6		qGL 6	7.55±0.21	0.15	0.002
N ^a		—		长宽比 Ratio of length to width		2.17±0.04
S4	RM5928--RM6266--RM157	1.43	3		qRLW3	2.31±0.07	0.07	0.0003
S5	RM5688--RM1155--RM17078	2.95	4		qRLW4	2.33±0.11	0.08	0.0007
S7	RM103--RM494--long arm end	0.20	6		qRLW6	2.50±0.12	0.16	9.94E-07
N ^a		—		结实率 Seed-setting rate (%)		92.73±3.59
S5	RM5688--RM1155--RM119	2.95	4	结实率 Seed-setting rate (%)	qSSR4	81.24±4.32	-5.75	0.0002
N ^a		—		每穗总粒数 Spikelets per panicle		91.76±8.36
S5	RM5688--RM1155--RM17078	2.95	4		qSP4	104.00±9.37	6.12	0.006
S7	RM103--RM494--long arm end	0.20	6		qSP6	117.6±10.12	12.92	1.30E-05
N ^a		—		千粒重 1000-grain weight (g)		24.07±0.53
S4	RM5928--RM6266--RM157	1.43	3	千粒重 1000-grain weight (g)	qGWT3	27.56±0.47	1.75	7.11E-10

表3

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性状 Trait	平均值±标准差(亲本) Mean±SD (parents)		F₂群体 F₂ population
性状 Trait	日本晴 Nipponbare	Z746	平均值±标准差 Mean±SD	范围 Range	偏度 Skewness	峰度 Kurtosis
株高Plant height (cm)	87.93±5.32	96.70±2.11^**	89.61±8.43	78.00-124.00	2.16	5.33
有效穗数Panicle number per panicle	18.40±5.55	17.70±5.42	14.41±4.56	6.00-28.00	0.67	0.23
穗长Panicle length (cm)	19.68±0.88	20.64±0.62	19.38±1.70	15.86-29.25	1.93	6.44
一次枝梗数Number of primary branch	7.42±0.43	8.05±0.67^*	8.36±1.49	6.67-14.47	2.75	7.62
二次枝梗数Number of secondary branch	14.62±1.67	22.99±2.42^**	14.59±4.38	7.17-34.73	2.20	6.71
粒长Grain length (mm)	7.40±0.08	8.93±0.19^**	7.71±0.57	7.03-9.42	1.16	0.19
粒宽Grain width (mm)	3.44±0.06	2.85±0.11^**	3.26±0.56	2.63-3.48	10.85	132.05
长宽比Ratio of length to width	2.15±0.03	3.13±0.15^**	2.40±0.34	2.11-3.53	0.56	4.21
结实率Seed-setting rate (%)	91.79±2.02	89.01±2.34^*	87.00±11.02	30.00-96.05	-3.25	11.54
每穗总粒数Spikelets per panicle	97.70±23.84	122.66±10.99^*	91.75±18.10	62.56-182.67	2.24	7.62
每穗实粒数Grains per panicle	89.68±22.56	109.23±10.58^*	78.77±15.17	26.11-166.73	0.90	7.86
千粒重1000-grain weight (g)	25.41±0.91	26.09±0.71	25.34±1.58	18.33-30.10	0.04	2.15
单株产量Yield per plant (g)	38.44±10.23	44.42±15.42	28.87±11.46	9.91-64.25	0.89	0.36

性状 Trait	QTL	染色体 Chr.	连锁标记 Linked marker	估计效应 Additive	贡献率 Var. (%)	P值 P-value
株高 Plant height (cm)	qPH2-1	2	RM1075	-5.06	15.06	< 0.0001
	qPH2-2	2	RM6378	4.11	13.70	0.0037
	qPH3-1	3	RM14389	3.17	2.71	0.0108
	qPH3-2	3	RM6266	1.92	5.06	0.0258
	qPH4	4	RM1155	4.71	16.67	< 0.0001
	qPH6-1	6	RM7412	3.01	13.59	0.0009
	qPH6-2	6	RM103	-4.13	26.98	0.0075
穗长 Panicle length (cm)	qPL2	2	RM1075	-0.99	13.12	< 0.0001
	qPL3	3	RM6266	0.39	4.85	0.0346
	qPL4	4	RM1155	0.49	4.16	0.0276
	qPL6	6	RM7412	0.64	13.81	0.0011
一次枝梗数 Number of primary branch	qNPB2-1	2	RM1075	-0.49	4.19	0.0198
	qNPB2-2	2	RM6378	0.70	11.59	0.0074
	qNPB2-3	2	RM13262	-0.47	5.43	0.0449
	qNPB3	3	RM14389	0.46	5.92	0.0424
	qNPB4	4	RM1155	1.10	27.43	< 0.0001
	qNPB6-1	6	RM7412	0.57	14.32	0.0007
	qNPB6-2	6	RM103	-0.87	35.15	0.0023
	qNPB6-3	6	RM494	0.63	18.95	0.0165
二次枝梗数 Number of secondary branch	qNSB2-1	2	RM1075	-1.42	3.35	0.0366
	qNSB2-2	2	RM6378	1.76	7.10	0.0284
	qNSB4	4	RM1155	2.01	8.73	0.0015
	qNSB6	6	RM7412	1.80	13.81	0.0011
粒长 Grain length (mm)	qGL2	2	RM1075	-0.11	3.63	0.0302
	qGL3	3	RM6266	0.34	60.28	< 0.0001
	qGL4	4	RM1155	0.26	27.47	< 0.0001
	qGL6	6	RM7412	0.08	4.77	0.0467
长宽比 Ratio of length to width	qRLW2	2	RM13262	0.16	12.97	0.0017
	qRLW3	3	RM6266	0.13	14.23	0.0004
	qRLW4	4	RM1155	0.15	10.41	0.0005
	qRLW6	6	RM7412	0.08	5.30	0.0414
结实率 Seed-setting rate (%)	qSSR4	4	RM1155	-0.07	17.33	< 0.0001
每穗粒数 Spikelets per panicle	qSP4	4	RM1155	6.24	4.73	0.0122
每穗粒数 Spikelets per panicle	qSP6	6	RM7412	4.33	4.51	0.0481
千粒重 1000-grain weight (g)	qGWT2	2	RM13262	-0.88	14.82	0.0009
千粒重 1000-grain weight (g)	qGWT3	3	RM6266	1.13	40.50	< 0.0001

优良水稻染色体片段代换系Z746的鉴定及重要农艺性状QTL定位及其验证

Identification of an excellent rice chromosome segment substitution line Z746 and QTL mapping and verification of important agronomic traits

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