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作物学报 ›› 2021, Vol. 47 ›› Issue (3): 438-450.doi: 10.3724/SP.J.1006.2021.04063
韩贝1(
), 王旭文2(), 李保奇1, 余渝2, 田琴2,*(), 杨细燕1,*()
HAN Bei1(), WANG Xu-Wen2(), LI Bao-Qi1, YU Yu2, TIAN Qin2,*(), YANG Xi-Yan1,*()
摘要：
干旱是导致全世界棉花严重减产、纤维品质下降的重要因素, 因此获得高产、优质、耐旱的棉花新品种一直是棉花的育种目标。本研究选取217份陆地棉栽培种组成的自然群体为研究对象, 采用全生育期处理组灌水量为对照组50%的干旱胁迫处理, 并在处理后期对217份材料的株高、衣分、单铃重等18个性状进行2年2点的表型鉴定, 干旱胁迫后, 群体间响应差异明显, 多个表型性状在对照和处理间表现显著差异。通过BLUP分析表型数据并计算各性状的抗旱系数; 全基因组范围选取的214对多态性SSR分子标记扫描群体, 共检测到393个多态性位点, 基因多样性系数平均值为0.402, 范围为0.072~0.631, PIC值平均为0.329, 范围为0.070~0.560; 群体结构分析表明, 该群体可分为2个亚群。用上述SSR标记分别对18个性状的抗旱系数进行关联分析, 共关联到76个极显著位点(P<0.01), 表型变异解释率为2.930%~7.218%, 其中共有14个标记位点能同时被2种或以上性状检测到。研究结果可为后期棉花杂交育种亲本选择及抗旱分子标记辅助育种提供理论基础及参考依据。
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