作物学报 ›› 2021, Vol. 47 ›› Issue (4): 770-779.doi: 10.3724/SP.J.1006.2021.03031
王蕊1(), 施龙建1,2(), 田红丽1, 易红梅1, 杨扬1, 葛建镕1, 范亚明1, 任洁1, 王璐1, 陆大雷2, 赵久然1,*(), 王凤格1,*()
WANG Rui1(), SHI Long-Jian1,2(), TIAN Hong-Li1, YI Hong-Mei1, YANG Yang1, GE Jian-Rong1, FAN Ya-Ming1, REN Jie1, WANG Lu1, LU Da-Lei2, ZHAO Jiu-Ran1,*(), WANG Feng-Ge1,*()
摘要:
纯度是玉米种子质量的重要指标之一, 尤其杂交种自交株是影响田间产量的关键因素。KASP (kompetitive allele specific PCR)技术具有高通量、低成本的优点, 适用于种子纯度检测。本研究基于12套杂交种及其父母本的三联体样本及335份玉米杂交种国家审定标准样品SNP指纹, 从384个SNP基础位点筛选获得60个候选位点, 位点转化为KASP引物的成功率为95%。综合考虑引物双亲互补率、多态性、稳定性和分型效果等多项指标, 最终确定20个引物作为玉米杂交种纯度鉴定的核心引物, 能够有效鉴定99.7%供试样品纯度。对于待测样品京科968通过SNP-DNA指纹数据库查询, 并选择双亲互补型引物进行纯度鉴定。在检测的110个个体中, 共检出1个自交苗和2个异型株, 纯度为97.3%。同时, 基于纯度核心引物对批量样品检测建立高通量纯度检测方案, 具有快捷、准确、高通量和低成本的特点, 为政府监管和企业提供了更多纯度鉴定方案的选择。
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