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作物学报 ›› 2021, Vol. 47 ›› Issue (11): 2147-2162.doi: 10.3724/SP.J.1006.2021.04271

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

紫甘薯SSR标记遗传图谱构建与重要农艺性状QTL定位

马猛(), 闫会, 高闰飞, 后猛, 唐维, 王欣, 张允刚, 李强*()   

  1. 中国农业科学院甘薯研究所 / 江苏徐淮地区徐州农业科学研究所 / 农业农村部甘薯生物学与遗传育种重点实验室, 江苏徐州 221131
  • 收稿日期:2020-12-13 接受日期:2021-03-19 出版日期:2021-11-12 网络出版日期:2021-04-12
  • 通讯作者: 李强
  • 作者简介:E-mail: 1325428037@qq.com
  • 基金资助:
    国家重点研发计划项目(2019YFD1001300);国家重点研发计划项目(2019YFD1001304);国家现代农业产业技术体系建设专项(CARS-10, 甘薯)

Construction linkage maps and identification of quantitative trait loci associated with important agronomic traits in purple-fleshed sweetpotato

MA Meng(), YAN Hui, GAO Run-Fei, KOU Meng, TANG Wei, WANG Xin, ZHANG Yun-Gang, LI Qiang*()   

  1. Sweetpotato Research Institute, Chinese Academy of Agricultural Sciences / Xuzhou Institute of Agricultural Sciences in Jiangsu Xuhuai District / Key Laboratory of Biology and Genetic Breeding of Sweetpotato, Ministry of Agriculture and Rural Affairs, Xuzhou 221131, Jiangsu, China
  • Received:2020-12-13 Accepted:2021-03-19 Published:2021-11-12 Published online:2021-04-12
  • Contact: LI Qiang
  • Supported by:
    National Key Research and Development Program of China(2019YFD1001300);National Key Research and Development Program of China(2019YFD1001304);China Agriculture Research System(CARS-10, 甘薯)

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摘要：

理想农艺性状是甘薯育种的重要目标, 而选择甘薯理想农艺性状的育种手段还很缺乏。本研究以分枝数多、蔓长中等、高产紫肉甘薯品种徐紫薯8号为母本, 分枝数少、长蔓、中等产量白肉甘薯品种美国红为父本, 以F1代分离群体的274个单株为作图群体, 利用SSR分子标记技术, 构建了甘薯分子连锁图谱, 能够加密已有的遗传图谱。其中母本图谱包含24个连锁群(linkage groups, LGs), 图谱总长1325.8 cM, 标记间平均距离9.2 cM; 父本图谱包含21个LGs, 图谱总长1088.6 cM, 标记间平均距离8.2 cM。通过复合区间作图法对甘薯地上部分枝数、茎蔓直径、最长蔓长、叶柄长度和节间长度5个重要农艺性状进行QTL分析, 检测到1个与分枝数相关的QTL, 解释表型变异的53.2%; 1个与茎蔓直径相关的QTL, 解释表型变异的16.7%; 2个与最长蔓长相关的QTL, 解释表型变异的9.5%和13.7%; 2个与叶柄长度相关定位的重要农艺性状QTL, 可以开发与其连锁的分子标记, 辅助室内早代苗期筛选具有理想农艺性状的株系, 从而提高田间选择效率的QTL, 解释表型变异的8.8%和11.3%; 5个与节间长度相关的QTL, 解释表型变异的9.6%~28.1%。利用定位的重要农艺性状QTL,可以开发与其连锁的分子标记,辅助室内早代苗期筛选具有理想农艺性状的株系,从而提高田间选择效率。。

关键词: 甘薯, 分枝数, 茎蔓直径, 最长蔓长, 叶柄长度, 节间长度, QTL

Abstract:

Ideal agronomic traits are the important objectives in sweetpotato breeding, but the breeding methods are still lacking. We constructed linkage maps using a mapping population of 274 individuals derived from a cross between the female parent Xuzishu 8 (a purple-fleshed cultivar with many branches, medium vine, and high yield) and the male parent Meiguohong (a white-fleshed cultivar with few branches, long vine, and medium yield) by simple sequence repeats (SSR) markers in this study. The female parent map contained 24 linkage groups, and covered 1325.8 cM with an average marker interval of 9.2 cM. The male parent map contained 21 linkage groups, and covered 1088.6 cM with an average marker interval of 8.2 cM. The maps could increase the density of existing genetic maps. Using the composite interval mapping, we analyzed five important agronomic traits, including branch number, vine diameter, longest vine length, petiole length, and internode length in sweetpotato, thus identified one QTL related to branch number explaining the phenotypic variance of 53.2%, one QTL related to internode diameter explaining the phenotypic variance of 16.7%, two QTLs related to longest vine length explaining the phenotypic variance of 9.5% and 13.7%, two QTLs related to petiole length explaining the phenotypic variance of 8.8% and 11.3%, and five QTLs related to internode length explaining the phenotypic variance of 9.6%-28.1%. The QTLs can be used to develop molecular markers and assist the screening of plants with ideal agronomic traits at early seedling stage, thus improved the efficiency of field selection.

Key words: sweetpotato, the number of branches, vine diameter, the longest vine length, petiole length, internode length, QTLs

图1

徐紫薯8号分子连锁图谱"

图2

美国红分子连锁图谱"

表1

甘薯分枝数双亲值及其在F1群体中的分布"

年份
Year		 母本(个)
Maternal		 父本(个)
Paternal		 最大值(个)
Max.		 最小值(个)
Min.		 平均值(个)
Mean		 标准差
SD		 偏度
Skewness		 峰度
Kurtosis
2019 14.83 6.67 75.00 7.00 23.00 10.47 1.37 3.29
2020 14.20 8.00 25.80 2.33 11.95 3.70 0.64 1.03
平均Average 14.52 7.33 66.00 6.20 18.14 8.56 2.04 6.41

图3

分枝数在F1分离群体中的频率分布图"

图4

甘薯分枝数QTL在分子连锁图谱上的分布"

表2

甘薯茎蔓直径双亲值及其在F1群体中的分布"

年份
Year		 母本
Maternal (mm)		 父本
Paternal (mm)		 最大值
Max. (mm)		 最小值
Min. (mm)		 平均值
Mean (mm)		 标准差
SD		 偏度
Skewness		 峰度
Kurtosis
2019 3.93 6.88 7.04 2.52 5.17 0.80 -0.21 0.39
2020 4.19 6.90 7.26 2.80 5.23 0.72 0.06 0.75
平均Average 4.06 6.89 7.19 2.96 5.19 0.72 -0.13 0.54

图5

茎蔓直径在F1分离群体中的频率分布图"

图6

甘薯茎蔓直径QTL在分子连锁图谱上的分布"

表3

甘薯最长蔓长双亲值及其在F1群体中的分布"

年份
Year		 母本
Maternal (cm)		 父本
Paternal (cm)		 最大值
Max. (cm)		 最小值
Min. (cm)		 平均值
Mean (cm)		 标准差
SD		 偏度
Skewness		 峰度
Kurtosis
2019 178.33 185.00 425.00 75.00 178.09 52.82 0.93 2.41
2020 182.00 195.00 361.00 77.00 172.74 47.83 0.50 0.60
平均Average 180.17 190.00 393.00 75.00 174.68 43.86 0.63 2.20

图7

最长蔓长在F1分离群体中的频率分布图"

表4

甘薯最长蔓长的QTL分析"

QTL名称
QTL name		 连锁图谱
Linkage group		 QTL位置
QTL location (cM)		 年份
Year		 遗传效应
Genetic effect		 贡献率
R2 (%)
qVLm-1p Meiguohong (2) 5.006 2020 正向Positive 18.0
平均Average 13.7
qVLf-1p Xuzishu 8 (18) 15.775 2019 正向Positive 12.4
平均Average 9.5

图8

甘薯最长蔓长QTL在分子连锁图谱上的分布"

表5

甘薯叶柄长度双亲值及其在F1群体中的分布"

年份
Year		 母本
Maternal (cm)		 父本
Paternal (cm)		 最大值
Max. (cm)		 最小值
Min. (cm)		 平均值
Mean (cm)		 标准差
SD		 偏度
Skewness		 峰度
Kurtosis
2019 14.17 15.48 27.98 5.80 16.74 4.18 0.47 -0.24
2020 17.94 10.92 26.64 8.80 18.60 3.16 -0.05 0.10
平均Average 16.05 13.20 25.24 5.80 17.54 3.38 0.02 -0.14

图9

叶柄长度在F1分离群体中的频率分布图"

表6

甘薯叶柄长度的QTL分析"

QTL名称
QTL name		 连锁图谱
Linkage group		 QTL位置
QTL location (cM)		 年份
Year		 遗传效应
Genetic effect		 贡献率
R2 (%)
qPLf-1p Xuzishu 8 (1) 40.483 2019 正向Positive 6.5
平均Average 11.3
qPLf-2p Xuzishu 8 (18) 41.837 2020 正向Positive 6.5
平均Average 8.8

图10

甘薯叶柄长度QTL在分子连锁图谱上的分布"

表7

甘薯节间长度双亲值及其在F1群体中的分布"

年份
Year		 母本
Maternal (cm)		 父本
Paternal (cm)		 最大值
Max. (cm)		 最小值
Min. (cm)		 平均值
Mean (cm)		 标准差
SD		 偏度
Skewness		 峰度
Kurtosis
2019 4.08 3.95 10.35 2.00 4.16 1.45 1.46 2.78
2020 4.98 3.48 9.46 3.10 5.39 1.17 0.63 0.51
平均Average 4.53 3.72 9.55 2.40 4.73 1.23 0.76 1.50

图11

节间长度在F1分离群体中的频率分布图"

表8

甘薯节间长度的QTL分析"

QTL名称
QTL name		 连锁图谱
Linkage group		 QTL位置
QTL location (cM)		 年份
Year		 遗传效应
Genetic effect		 贡献率
R2 (%)
qILm-1p Meiguohong (2) 5.006 2020 正向Positive 9.8
平均Average 9.6
qILf-1p Xuzishu 8 (1) 40.483 2019 正向Positive 15.0
平均Average 15.8
qILf-2n Xuzishu 8 (2) 13.470 2019 负向Negative 13.1
平均Average 16.1
qILf-3n Xuzishu 8 (9) 24.405 2019 负向Negative 53.6
平均Average 28.1
qILf-4p Xuzishu 8 (18) 21.380 2019 正向Positive 43.5
平均Average 20.6

图12

甘薯节间长度QTL在分子连锁图谱上的分布"

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