欢迎访问作物学报,今天是

作物学报 ›› 2022, Vol. 48 ›› Issue (1): 27-39.doi: 10.3724/SP.J.1006.2022.04281

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

甘蓝型油菜角果数突变体基因的定位及候选基因分析

赵改会1(), 李书宇2, 詹杰鹏1, 李晏斌3, 师家勤1,*(), 王新发1, 王汉中1   

  1. 1中国农业科学院油料作物研究所, 湖北武汉 430062
    2江西省农业科学院作物研究所, 江西南昌 330200
    3武汉市农业技术推广中心, 湖北武汉 430400
  • 收稿日期:2020-12-27 接受日期:2021-04-14 出版日期:2022-01-12 网络出版日期:2021-06-15
  • 通讯作者: 师家勤
  • 作者简介:E-mail: 3468382960@qq.com第一联系人:**同等贡献
  • 基金资助:
    江西省自然科学基金项目(20202BABL205016);湖北省自然科学基金重点(杰青)项目(2018CFA075);优质高产多抗油菜新品种选育项目(2018ABA087);国家现代农业产业技术体系建设专项资助(CARS-13)

Mapping and candidate gene analysis of silique number mutant in Brassica napus L.

ZHAO Gai-Hui1(), LI Shu-Yu2, ZHAN Jie-Peng1, LI Yan-Bin3, SHI Jia-Qin1,*(), WANG Xin-Fa1, WANG Han-Zhong1   

  1. 1Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, Hubei, China
    2Crop Research Institute, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, Jiangxi, China
    3Wuhan Agricultural Technology Extension Center, Wuhan 430400, Hubei, China
  • Received:2020-12-27 Accepted:2021-04-14 Published:2022-01-12 Published online:2021-06-15
  • Contact: SHI Jia-Qin
  • About author:First author contact:** Contributed equally to this work
  • Supported by:
    Natural Science Foundation of Jiangxi Province(20202BABL205016);Hubei Provincial Natural Science Foundation (Outstanding Youth)(2018CFA075);Breeding of New Rapeseed Varieties with High Quality, High Yield and Multiple Resistance(2018ABA087);China Agriculture Research System(CARS-13)

摘要:

角果数是油菜单株产量重要的构成因子之一, 其优异等位基因的发掘和利用对产量的提高至关重要。油菜中已定位到上百个角果数QTL, 但大多数效应不大且不稳定, 难以进行精细定位或克隆。本研究前期发掘到一个油菜突变体(No.7931), 其花序顶端在分化出约十朵花后即停止生长, 因而成熟期角果极少。利用该少角果突变体和多角果品系No.73290构建F2分离群体, 从中挑选角果数极端单株各30株进行BSA-seq, 在C02染色体检测到3个关联区间: 0~1.1 Mb、4.7~6.2 Mb、11.5~12.4 Mb。该候选区间在油菜参考基因组DarmorV8.1中有522个注释基因, 存在SNP或Indel差异且有同源注释的基因235个。在花芽分化初期, 选取两亲本(No.73290和No.7931)的茎尖分生组织进行RNA-seq, 总共鉴定到8958个差异表达基因(DEGs)。这些DEGs显著富集于20个生物学通路, 包括碳代谢、翻译、氨基酸代谢(和花芽分化高度相关)等, 其中99个位于关联区间。结合基因功能注释以及序列和表达差异分析确定了9个候选基因(BnaC02g00490.1D2BnaC02g01030.1D2BnaC02g01120.1D2BnaC02g00270.1D2BnaC02g02670. 1D2BnaC02g08680.1D2BnaC02g08890.1D2BnaC02g09480.1D2BnaC02g10490.1D2), 它们主要参与花序分生组织特性的维持和花器官的发育。上述研究结果为后续油菜角果数基因的精细定位和克隆奠定了坚实的基础。

关键词: 油菜, 油菜, 角果数突变体, 角果数突变体, 花芽分化, 花芽分化, BSA-seq, BSA-seq, RNA-seq, RNA-seq

Abstract:

The silique number is one of the important components of yield per plant in oilseed rape (Brassica napus L.) and the exploitation and utilization of its excellent alleles are essential to increase yield. More than hundreds of silique number QTLs have been mapped in oilseed rape, but they are difficult to be fine-mapped or cloned because of their moderate and unstable effects. A oilseed rape mutant (No.7931) was detected in previous study and it had few siliques at mature stage due to the stop growth after differentiation about 10 flowers on the top of inflorescence. A F2 segregating population consisting of 3400 individuals was constructed using this mutant and another more-silique lines No.73290. Among them, we performed BSA-seq on 30 individuals with extreme more- or less-siliques and detected three associated intervals of 0-1.1 Mb, 4.7-6.2 Mb, and 11.5-12.4 Mb on the C02 chromosome. These genomic intervals contained a total of 522 annotated genes in the reference genome DarmorV8.1, among which 235 genes had functional annotation and SNP/InDel variation. At the early stage of flower bud differentiation, the shoot apical meristems of two parents were subjected to RNA-seq, and a total of 8958 differentially expressed genes (DEGs) were detected. These DEGs were significantly enriched into 20 pathways, including carbohydrate metabolism, translation, and amino acid metabolism (highly associated with flower bud differentiation) and so on, among which 99 were located in the associated intervals. By the integration of gene functional annotation as well as sequence and expression variation analysis, a total of nine candidate genes (BnaC02g00490.1D2, BnaC02g01030.1D2, BnaC02g01120.1D2, BnaC02g00270.1D2, BnaC02g02670.1D2, BnaC02g08680.1D2, BnaC02g08890.1D2, BnaC02g09480.1D2, and BnaC02g10490.1D2) were identified, which were mainly involved in the maintenance of inflorescence meristems and the regulation of flower development. The above results lay the foundation for the following fine-mapping and cloning of the silique number mutant gene in oilseed rape.

Key words: Brassica napus L., Brassica napus L., silique number mutant, silique number mutant, flower bud differentiation, flower bud differentiation, BSA-seq, BSA-seq, RNA-seq, RNA-seq

图1

油菜少角果突变体No.7931和多角果品系No.73290花期和成熟期花序的观察 A1: No.7931花期; A2: No.73290花期; B1: No.7931成熟期; B2: No.73290成熟期。"

图1

油菜少角果突变体No.7931和多角果品系No.73290花期和成熟期花序的观察 A1: No.7931花期; A2: No.73290花期; B1: No.7931成熟期; B2: No.73290成熟期。"

表1

No.7931和No.73290表型数据统计分析"

亲本
Parent
主花序花器官数目
FNm
主花序角果数
SNm
株高
PH
No.7931 8.33±1.24 7.67±0.94 154.3±2.06
No.73290 199.3±1.24 93.67±2.49 150.4±1.57
t检验值t-test value 5.45E-09 6.91E-07 0.0170

表1

No.7931和No.73290表型数据统计分析"

亲本
Parent
主花序花器官数目
FNm
主花序角果数
SNm
株高
PH
No.7931 8.33±1.24 7.67±0.94 154.3±2.06
No.73290 199.3±1.24 93.67±2.49 150.4±1.57
t检验值t-test value 5.45E-09 6.91E-07 0.0170

图2

油菜角果数突变体的显微观察 A、B、C、D分别代表花原基分化、花萼分化、花瓣原基分化(蕾轴伸长)与现蕾早期4个时期; 1、2分别代表No.7931和No.73290。"

图2

油菜角果数突变体的显微观察 A、B、C、D分别代表花原基分化、花萼分化、花瓣原基分化(蕾轴伸长)与现蕾早期4个时期; 1、2分别代表No.7931和No.73290。"

图3

候选区间峰图 横坐标表示Δ(SNP-index)在染色体上的位置, 纵坐标表示Δ(SNP-index)的数值。"

图3

候选区间峰图 横坐标表示Δ(SNP-index)在染色体上的位置, 纵坐标表示Δ(SNP-index)的数值。"

附图1

样本间相关系数热图"

附图1

样本间相关系数热图"

附表1

qRT-PCR引物"

基因
Gene
正向引物
Forward primer (5°-3°)
反向引物
Reverse primer (5°-3°)
BnaA02G0314300ZS CCGACACGCTTCAGAAGGTC AGAGATCCCGCTTCGACTCC
BnaC03G0027000ZS TGAGAACACGCCGGTCAAAG CCTCGCGTTCTCTCTCTCCA
BnaC03G0100100ZS CTGATGGCCGTAGAGCATGC AGCCATCTCCTGTTGTTGCA
BnaC03G0091000ZS TCCCACTCTACCCTGCACTG GACGTTGTTCACATTCGCGC
BnaC02G0283700ZS ACAACATGGCCCTGGAAACTG CTTGGAGGCGGATGATCGTT
BnaC02G0139700ZS CGGCGGAGGTACAGACATTT TCGTCATGATGAGCCTCTCCT
BnaC02G0175400ZS GCGCCTCGTATCCATTCTCG CCTGAAGTTGTGGCGAGCTT
BnaA01G0077800ZS CCACCGTCATGTCTTCCTTCC ACGAGTTGGAAGTGTGCGTT
BnaA01G0061200ZS GGGAACGGCTTAGATGGTGC TTGCTAGTACCAGGGCTGCT
BnaC02G0013900ZS CATTGGCTCGTCATGAACATC TTCACGAGTGTTGAACTGATCC
BnaC02G0159100ZS CTGTCACTGGAAACCACCCG TCAATCGACCATGGCAAGCA
BnaA01G0009100ZS TCTGCTCTGAACGCGACCAA ACCAGCCAAAGAACCAGGGT
BnaA01G0037200ZS TCCTCAACTGTGCCGACATGT AAAGCCGTCGTCAATCTCGC
BnaC05G0266700ZS AGACTACGTGAAGCAGCCGA CTCCAGCTTCCGACCAGTCT
BraActin CTGGAATTGCTGACCGTATGAG ATCTGTTGGAAAGTGCTGAGGG

附表1

qRT-PCR引物"

基因
Gene
正向引物
Forward primer (5°-3°)
反向引物
Reverse primer (5°-3°)
BnaA02G0314300ZS CCGACACGCTTCAGAAGGTC AGAGATCCCGCTTCGACTCC
BnaC03G0027000ZS TGAGAACACGCCGGTCAAAG CCTCGCGTTCTCTCTCTCCA
BnaC03G0100100ZS CTGATGGCCGTAGAGCATGC AGCCATCTCCTGTTGTTGCA
BnaC03G0091000ZS TCCCACTCTACCCTGCACTG GACGTTGTTCACATTCGCGC
BnaC02G0283700ZS ACAACATGGCCCTGGAAACTG CTTGGAGGCGGATGATCGTT
BnaC02G0139700ZS CGGCGGAGGTACAGACATTT TCGTCATGATGAGCCTCTCCT
BnaC02G0175400ZS GCGCCTCGTATCCATTCTCG CCTGAAGTTGTGGCGAGCTT
BnaA01G0077800ZS CCACCGTCATGTCTTCCTTCC ACGAGTTGGAAGTGTGCGTT
BnaA01G0061200ZS GGGAACGGCTTAGATGGTGC TTGCTAGTACCAGGGCTGCT
BnaC02G0013900ZS CATTGGCTCGTCATGAACATC TTCACGAGTGTTGAACTGATCC
BnaC02G0159100ZS CTGTCACTGGAAACCACCCG TCAATCGACCATGGCAAGCA
BnaA01G0009100ZS TCTGCTCTGAACGCGACCAA ACCAGCCAAAGAACCAGGGT
BnaA01G0037200ZS TCCTCAACTGTGCCGACATGT AAAGCCGTCGTCAATCTCGC
BnaC05G0266700ZS AGACTACGTGAAGCAGCCGA CTCCAGCTTCCGACCAGTCT
BraActin CTGGAATTGCTGACCGTATGAG ATCTGTTGGAAAGTGCTGAGGG

图4

转录组测序与实时荧光定量PCR结果的比较"

图4

转录组测序与实时荧光定量PCR结果的比较"

图5

差异表达基因GO富集分析"

图5

差异表达基因GO富集分析"

图6

差异表达基因的功能分类"

图6

差异表达基因的功能分类"

图7

差异表达基因的KEGG分析"

图7

差异表达基因的KEGG分析"

表2

关联区间内的候选基因预测"

油菜基因编号
B. napus ID
拟南芥
基因名
Name of
A. thaliana
拟南芥基因
编号
A. thaliana ID
位置Position
(Mb)
基因注释
Gene annotation
序列差异
Sequence difference
差异表达基因
Differential expressed genes
BnaC02g00490.1D2 FLC AT5g10140 0.208 K盒区和MADS转录因子家族蛋白
K-box region and MADS-box transcription factor family protein
有Yes 是No
BnaC02g01030.1D2 TPR1 AT1G80490 0.465 TPL相关的基因1 TOPLESS-related 1 无No 否No
BnaC02g01120.1D2 AGAL2 AT5G08370 0.493 α-半乳糖苷酶2 Alpha-galactosidase 2 无No 否No
BnaC02g00270.1D2 AIL6 AT5G10510 0.996 ANT基因6
AINTEGUMENTA-LIKE 6
有Yes 否No
BnaC02g02670.1D2 TFL1 AT5G03840 1.10 磷脂酰乙醇胺结合蛋白
Phosphatidylethanolamine-binding-protein
无No 是Yes
BnaC02g08680.1D2 COL1 AT5G15850 4.83 CO基因1 CONSTANS-like 1 有Yes 否No
BnaC02g08890.1D2 ELF9 AT5G16260 4.96 RNA结合(RRM/RBD/RNP基序)家族蛋白RNA binding (RRM/RBD/RNP motifs) family protein 有Yes 否No
BnaC02g09480.1D2 AIM1 AT4G29010 5.23 烯酰辅酶A水合酶/异构酶家族
Enoyl-CoA hydratase/isomerase family
有Yes 否No
BnaC02g10490.1D2 CHR17 AT5G18620 5.97 染色质重塑因子17
Chromatin remodeling factor 17
无No 是Yes

表2

关联区间内的候选基因预测"

油菜基因编号
B. napus ID
拟南芥
基因名
Name of
A. thaliana
拟南芥基因
编号
A. thaliana ID
位置Position
(Mb)
基因注释
Gene annotation
序列差异
Sequence difference
差异表达基因
Differential expressed genes
BnaC02g00490.1D2 FLC AT5g10140 0.208 K盒区和MADS转录因子家族蛋白
K-box region and MADS-box transcription factor family protein
有Yes 是No
BnaC02g01030.1D2 TPR1 AT1G80490 0.465 TPL相关的基因1 TOPLESS-related 1 无No 否No
BnaC02g01120.1D2 AGAL2 AT5G08370 0.493 α-半乳糖苷酶2 Alpha-galactosidase 2 无No 否No
BnaC02g00270.1D2 AIL6 AT5G10510 0.996 ANT基因6
AINTEGUMENTA-LIKE 6
有Yes 否No
BnaC02g02670.1D2 TFL1 AT5G03840 1.10 磷脂酰乙醇胺结合蛋白
Phosphatidylethanolamine-binding-protein
无No 是Yes
BnaC02g08680.1D2 COL1 AT5G15850 4.83 CO基因1 CONSTANS-like 1 有Yes 否No
BnaC02g08890.1D2 ELF9 AT5G16260 4.96 RNA结合(RRM/RBD/RNP基序)家族蛋白RNA binding (RRM/RBD/RNP motifs) family protein 有Yes 否No
BnaC02g09480.1D2 AIM1 AT4G29010 5.23 烯酰辅酶A水合酶/异构酶家族
Enoyl-CoA hydratase/isomerase family
有Yes 否No
BnaC02g10490.1D2 CHR17 AT5G18620 5.97 染色质重塑因子17
Chromatin remodeling factor 17
无No 是Yes
[1] 刘成, 冯中朝, 肖唐华, 马晓敏, 周广生, 黄凤洪, 李加纳, 王汉中. 我国油菜产业发展现状、潜力及对策. 中国油料作物学报, 2019, 41:485-489.
刘成, 冯中朝, 肖唐华, 马晓敏, 周广生, 黄凤洪, 李加纳, 王汉中. 我国油菜产业发展现状、潜力及对策. 中国油料作物学报, 2019, 41:485-489.
Liu C, Feng Z C, Xiao T H, Ma X M, Zhou G S, Huang F H, Li J N, Wang H Z. Development, potential and adaptation of Chinese rapeseed industry. Chin J Oil Crop Sci, 2019, 41:485-489 (in Chinese with English abstract).
Liu C, Feng Z C, Xiao T H, Ma X M, Zhou G S, Huang F H, Li J N, Wang H Z. Development, potential and adaptation of Chinese rapeseed industry. Chin J Oil Crop Sci, 2019, 41:485-489 (in Chinese with English abstract).
[2] 王汉中. 以新需求为导向的油菜产业发展战略. 中国油料作物学报, 2018, 40:613-617.
王汉中. 以新需求为导向的油菜产业发展战略. 中国油料作物学报, 2018, 40:613-617.
Wang H Z. New-demand oriented oilseed rape industry developing strategy. Chin J Oil Crop Sci, 2018, 40:613-617 (in Chinese with English abstract).
Wang H Z. New-demand oriented oilseed rape industry developing strategy. Chin J Oil Crop Sci, 2018, 40:613-617 (in Chinese with English abstract).
[3] 范成明, 田建华, 胡赞民, 王珏, 吕慧颖, 葛毅强, 魏珣, 邓向东, 张蕾颖, 杨维才. 油菜育种行业创新动态与发展趋势. 植物遗传资源学报, 2018, 19:447-454.
范成明, 田建华, 胡赞民, 王珏, 吕慧颖, 葛毅强, 魏珣, 邓向东, 张蕾颖, 杨维才. 油菜育种行业创新动态与发展趋势. 植物遗传资源学报, 2018, 19:447-454.
Fan C M, Tian J H, Hu Z M, Wang J, Lyu H Y, Ge Y Q, Wei X, Deng X D, Zhang L Y, Yang W C. Advances of oilseed rape breeding. J Plant Genet Resour, 2018, 19:447-454 (in Chinese with English abstract).
Fan C M, Tian J H, Hu Z M, Wang J, Lyu H Y, Ge Y Q, Wei X, Deng X D, Zhang L Y, Yang W C. Advances of oilseed rape breeding. J Plant Genet Resour, 2018, 19:447-454 (in Chinese with English abstract).
[4] 宋稀, 刘凤兰, 郑普英, 张学昆, 陆光远, 付桂萍, 程勇. 高密度种植专用油菜重要农艺性状与产量的关系分析. 中国农业科学, 2010, 43:1800-1806.
宋稀, 刘凤兰, 郑普英, 张学昆, 陆光远, 付桂萍, 程勇. 高密度种植专用油菜重要农艺性状与产量的关系分析. 中国农业科学, 2010, 43:1800-1806.
Song X, Liu F L, Zheng P Y, Zhang X K, Lu G Y, Fu G P, Cheng Y. Correlation analysis between agronomic traits and yield of rapeseed (Brassica napus L.) for high-density. Sci Agric Sin, 2010, 43:1800-1806 (in Chinese with English abstract).
Song X, Liu F L, Zheng P Y, Zhang X K, Lu G Y, Fu G P, Cheng Y. Correlation analysis between agronomic traits and yield of rapeseed (Brassica napus L.) for high-density. Sci Agric Sin, 2010, 43:1800-1806 (in Chinese with English abstract).
[5] 俞琦英, 赵伟明. 油菜区域试验产量性状及其稳定性分析. 浙江农业学报, 2010, 22:337-340.
俞琦英, 赵伟明. 油菜区域试验产量性状及其稳定性分析. 浙江农业学报, 2010, 22:337-340.
Yu Q Y, Zhao W M. Analysis of yield characters and its stability in rape regional trial of Zhejiang province. Acta Agric Zhejiangensis, 2010, 22:337-340 (in Chinese with English abstract).
Yu Q Y, Zhao W M. Analysis of yield characters and its stability in rape regional trial of Zhejiang province. Acta Agric Zhejiangensis, 2010, 22:337-340 (in Chinese with English abstract).
[6] 张芳. 我国油菜品种审定管理与育种趋势研究. 中国农业科学院硕士学位论文,北京, 2012.
张芳. 我国油菜品种审定管理与育种趋势研究. 中国农业科学院硕士学位论文,北京, 2012.
Zhang F. Management of Rapeseed and Breeding Trend in China. MS Thesis of Chinese Academy of Agricultural Sciences, Beijing,China, 2012 (in Chinese with English abstract).
Zhang F. Management of Rapeseed and Breeding Trend in China. MS Thesis of Chinese Academy of Agricultural Sciences, Beijing,China, 2012 (in Chinese with English abstract).
[7] 张锦芳, 周贤琼, 蒲晓斌, 李浩杰, 蒋梁材. 高产、双低杂交油菜产量构成因素与产量的相关分析. 西南农业学报, 2008, 27:939-941.
张锦芳, 周贤琼, 蒲晓斌, 李浩杰, 蒋梁材. 高产、双低杂交油菜产量构成因素与产量的相关分析. 西南农业学报, 2008, 27:939-941.
Zhang J F, Zhou X Q, Pu X B, Li H J, Jiang L C. Correlation analysis between yield components and yield per plant of high yield rapeseed hybrid. Southwest China J Agric Sci, 2008, 27:939-941 (in Chinese with English abstract).
Zhang J F, Zhou X Q, Pu X B, Li H J, Jiang L C. Correlation analysis between yield components and yield per plant of high yield rapeseed hybrid. Southwest China J Agric Sci, 2008, 27:939-941 (in Chinese with English abstract).
[8] Li S Y, Zhu Y Y, Rajeev K V, Zhan J P, Zheng X X, Shi J Q, Wang X F, Wang H Z. A systematic dissection of the mechanisms underlying the natural variation of silique number in rapeseed (Brassica napus L.) germplasm. Plant Biotechnol J, 2020, 18:568-580.
Li S Y, Zhu Y Y, Rajeev K V, Zhan J P, Zheng X X, Shi J Q, Wang X F, Wang H Z. A systematic dissection of the mechanisms underlying the natural variation of silique number in rapeseed (Brassica napus L.) germplasm. Plant Biotechnol J, 2020, 18:568-580.
[9] 李少钦, 王健胜, 张文学, 李殿荣, 郑磊, 田建华. 甘蓝型油菜优良亲本对杂种后代产量性状的遗传效应分析. 中国油料作物学报, 2011, 33:545-549.
李少钦, 王健胜, 张文学, 李殿荣, 郑磊, 田建华. 甘蓝型油菜优良亲本对杂种后代产量性状的遗传效应分析. 中国油料作物学报, 2011, 33:545-549.
Li S Q, Wang J S, Zhang W X, Li D R, Zheng L, Tian J H. Genetic analysis for hybrid yield traits using elite parents of Brassica napus L. Chin J Oil Crop Sci, 2011, 33:545-549 (in Chinese with English abstract).
Li S Q, Wang J S, Zhang W X, Li D R, Zheng L, Tian J H. Genetic analysis for hybrid yield traits using elite parents of Brassica napus L. Chin J Oil Crop Sci, 2011, 33:545-549 (in Chinese with English abstract).
[10] 郦美娟, 顾菊生. 油菜品种的产量和品质性状的稳定性分析. 浙江农业学报, 1989, 1(1):1-7.
郦美娟, 顾菊生. 油菜品种的产量和品质性状的稳定性分析. 浙江农业学报, 1989, 1(1):1-7.
Li M J, Gu J S. Stability analysis on the yield and quality of the rape varieties. Acta Agric Zhejiangensis, 1989, 1(1):1-7 (in Chinese with English abstract).
Li M J, Gu J S. Stability analysis on the yield and quality of the rape varieties. Acta Agric Zhejiangensis, 1989, 1(1):1-7 (in Chinese with English abstract).
[11] 王俊生, 张文学, 田建华, 李殿荣. 紧凑型油菜数量性状的遗传与杂种优势研究. 西北农业学报, 2006, 15(3):31-36.
王俊生, 张文学, 田建华, 李殿荣. 紧凑型油菜数量性状的遗传与杂种优势研究. 西北农业学报, 2006, 15(3):31-36.
Wang J S, Zhang W X, Tian J H, Li D R. Study on inheritance and heterosis of plant-type traits in compact rapeseed lines. Acta Agric Boreali-Occident Sin, 2006, 15(3):31-36 (in Chinese with English abstract).
Wang J S, Zhang W X, Tian J H, Li D R. Study on inheritance and heterosis of plant-type traits in compact rapeseed lines. Acta Agric Boreali-Occident Sin, 2006, 15(3):31-36 (in Chinese with English abstract).
[12] 张书芬. 甘蓝型油菜重要农艺和品质性状的杂种优势及遗传分析. 华中农业大学博士学位论文,湖北武汉, 2005.
张书芬. 甘蓝型油菜重要农艺和品质性状的杂种优势及遗传分析. 华中农业大学博士学位论文,湖北武汉, 2005.
Zhang S F. Heterosis and Genetic Analysis of Important Agronomic and Quality Characters in Brassica napus L. PhD Dissertation of Huazhong Agricultural University, Wuhan, Hubei, Chin, 2005 (in Chinese with English abstract).
Zhang S F. Heterosis and Genetic Analysis of Important Agronomic and Quality Characters in Brassica napus L. PhD Dissertation of Huazhong Agricultural University, Wuhan, Hubei, Chin, 2005 (in Chinese with English abstract).
[13] 高必军. 甘蓝型油菜napin基因启动子的克隆与几个重要农艺性状的初步QTL定位. 四川农业大学博士学位论文,四川成都, 2007.
高必军. 甘蓝型油菜napin基因启动子的克隆与几个重要农艺性状的初步QTL定位. 四川农业大学博士学位论文,四川成都, 2007.
Gao B J. Cloning of the napin Gene Promoter and Preliminary QTL of some Agronomical Import Traits in Brassica napus. PhD Dissertation of Sichuan Agricultural University, Chengdu, Sichuan,China, 2007 (in Chinese with English abstract).
Gao B J. Cloning of the napin Gene Promoter and Preliminary QTL of some Agronomical Import Traits in Brassica napus. PhD Dissertation of Sichuan Agricultural University, Chengdu, Sichuan,China, 2007 (in Chinese with English abstract).
[14] Radoev M, Becker H C, Ecke W. Genetic analysis of heterosis for yield and yield components in rapeseed (Brassica napus L.) by quantitative trait locus mapping. Genetics, 2008, 179:1547-1558.
Radoev M, Becker H C, Ecke W. Genetic analysis of heterosis for yield and yield components in rapeseed (Brassica napus L.) by quantitative trait locus mapping. Genetics, 2008, 179:1547-1558.
[15] Ding G D, Zhao Z K, Liao Y, Hu Y F, Shi L, Xu F S. Quantitative trait loci for seed yield and yield-related traits, and their responses to reduced phosphorus supply in Brassica napus. Ann Bot, 2012, 109:747-759.
Ding G D, Zhao Z K, Liao Y, Hu Y F, Shi L, Xu F S. Quantitative trait loci for seed yield and yield-related traits, and their responses to reduced phosphorus supply in Brassica napus. Ann Bot, 2012, 109:747-759.
[16] 孙美玉, 华玮, 刘静, 王新发, 刘贵华, 王汉中. 甘蓝型油菜主花序有效角果数QTL定位. 中国油料作物学报, 2013, 35:1-7.
孙美玉, 华玮, 刘静, 王新发, 刘贵华, 王汉中. 甘蓝型油菜主花序有效角果数QTL定位. 中国油料作物学报, 2013, 35:1-7.
Sun M Y, Hua W, Liu J, Wang X F, Liu G H, Wang H Z. QTLs for effective silique number of main inflorescence on rapeseed (Brassica napus L.). Chin J Oil Crop Sci, 2013, 35:1-7 (in Chinese with English abstract).
Sun M Y, Hua W, Liu J, Wang X F, Liu G H, Wang H Z. QTLs for effective silique number of main inflorescence on rapeseed (Brassica napus L.). Chin J Oil Crop Sci, 2013, 35:1-7 (in Chinese with English abstract).
[17] Shi J Q, Zhan J P, Yang Y H, Ye J, Huang S M, Li R Y, Wang X F, Wang H Z. Linkage and regional association analysis reveal two new tightly-linked major-QTLs for pod number and seed number per pod in rapeseed (Brassica napus L.). Sci Rep, 2015, 5:389-452.
Shi J Q, Zhan J P, Yang Y H, Ye J, Huang S M, Li R Y, Wang X F, Wang H Z. Linkage and regional association analysis reveal two new tightly-linked major-QTLs for pod number and seed number per pod in rapeseed (Brassica napus L.). Sci Rep, 2015, 5:389-452.
[18] 耿鑫鑫, 曾焕, 黄伊雪, 徐飞. 甘蓝型油菜主花序有效角果数QTL定位和候选基因筛选. 江苏农业科学, 2019, 47(7):38-41.
耿鑫鑫, 曾焕, 黄伊雪, 徐飞. 甘蓝型油菜主花序有效角果数QTL定位和候选基因筛选. 江苏农业科学, 2019, 47(7):38-41.
Geng X X, Zeng H, Huang Y X, Xu F. QTL mapping and candidate gene screening for effective pod number of main inflorescence ofBrassica napus L. Jiangsu Agric Sci, 2019, 47(7):38-41 (in Chinese with English abstract).
Geng X X, Zeng H, Huang Y X, Xu F. QTL mapping and candidate gene screening for effective pod number of main inflorescence ofBrassica napus L. Jiangsu Agric Sci, 2019, 47(7):38-41 (in Chinese with English abstract).
[19] 严贤诚, 陈立凯, 罗玉花, 罗文龙, 王慧, 郭涛, 陈志强. 水稻花器官数目突变体mf2的鉴定和基因定位. 作物学报, 2018, 44:169-176.
严贤诚, 陈立凯, 罗玉花, 罗文龙, 王慧, 郭涛, 陈志强. 水稻花器官数目突变体mf2的鉴定和基因定位. 作物学报, 2018, 44:169-176.
Yan X C, Chen L K, Luo Y H, Luo W L, Wang H, Guo T, Chen Z Q. Identification and gene mapping of a floral organ number mutant mf2 in rice(Oryza sativa). Acta Agron Sin, 2018, 44:169-176 (in Chinese with English abstract).
Yan X C, Chen L K, Luo Y H, Luo W L, Wang H, Guo T, Chen Z Q. Identification and gene mapping of a floral organ number mutant mf2 in rice(Oryza sativa). Acta Agron Sin, 2018, 44:169-176 (in Chinese with English abstract).
[20] Galli M, Gallavotti A. Expanding the regulatory network for meristem size in plants. Trends Genet, 2016, 32:372-383.
Galli M, Gallavotti A. Expanding the regulatory network for meristem size in plants. Trends Genet, 2016, 32:372-383.
[21] Tanaka W, Hirano H Y. Antagonistic action of TILLERS ABSENT1 and FLORAL ORGAN NUMBER2 regulates stem cell maintenance during axillary meristem development in rice. New Phytol, 2020, 225:974-984.
Tanaka W, Hirano H Y. Antagonistic action of TILLERS ABSENT1 and FLORAL ORGAN NUMBER2 regulates stem cell maintenance during axillary meristem development in rice. New Phytol, 2020, 225:974-984.
[22] 刘爽. 番茄心室形成研究进展. 农学学报, 2018, 8(12):63-66.
刘爽. 番茄心室形成研究进展. 农学学报, 2018, 8(12):63-66.
Liu S. Research advances of tomato locule formation. J Agric, 2018, 8(12):63-66 (in Chinese with English abstract).
Liu S. Research advances of tomato locule formation. J Agric, 2018, 8(12):63-66 (in Chinese with English abstract).
[23] Sunok M, Jung K H, LeeD F, Lee D Y, Jinwon L, Kyungsook A, Gynheung A. The rice FON1 gene controls vegetative and reproductive development by regulating shoot apical meristem size. Mol Cells, 2006, 21:147-52.
Sunok M, Jung K H, LeeD F, Lee D Y, Jinwon L, Kyungsook A, Gynheung A. The rice FON1 gene controls vegetative and reproductive development by regulating shoot apical meristem size. Mol Cells, 2006, 21:147-52.
[24] Liu C, Zhou Y, Zhang X C, Zhang J Y, Zhou Z Q, Weng G F, Wang Z H. Natural variation in the THICK TASSEL DWARF1 (TD1) gene in the regulation of maize(Zea mays L.) ear-related traits. Breed Sci, 2019, 69:323-331.
Liu C, Zhou Y, Zhang X C, Zhang J Y, Zhou Z Q, Weng G F, Wang Z H. Natural variation in the THICK TASSEL DWARF1 (TD1) gene in the regulation of maize(Zea mays L.) ear-related traits. Breed Sci, 2019, 69:323-331.
[25] Fumio T S, Zhuang Y, Jackson D. The fasciated ear2 gene encodes a leucine-rich repeat receptor-like protein that regulates shoot meristem proliferation in maize. Genes Dev, 2001, 15:2755-2766.
Fumio T S, Zhuang Y, Jackson D. The fasciated ear2 gene encodes a leucine-rich repeat receptor-like protein that regulates shoot meristem proliferation in maize. Genes Dev, 2001, 15:2755-2766.
[26] 刘洪岩, 岳淑婷, 张琳, 赵文静, 包颖. 影响番茄果实大小相关基因的研究进展. 曲阜师范大学学报(自然科学版), 2018, 44(2):81-85.
刘洪岩, 岳淑婷, 张琳, 赵文静, 包颖. 影响番茄果实大小相关基因的研究进展. 曲阜师范大学学报(自然科学版), 2018, 44(2):81-85.
Liu H Y, Yue S T, Zhang L, Zhao W J, Bao Y. Research progresses on related genes of affecting fruit size in tomato. J Qufu Nor Univ(Nat Sci), 2018, 44(2):81-85 (in Chinese with English abstract).
Liu H Y, Yue S T, Zhang L, Zhao W J, Bao Y. Research progresses on related genes of affecting fruit size in tomato. J Qufu Nor Univ(Nat Sci), 2018, 44(2):81-85 (in Chinese with English abstract).
[27] Hyten D L, Smith J R, Frederick R D, Tucker M L, Cregan P B. Bulked segregant analysis using the golden gate assay to locate the Rpp3 locus that confers resistance to soybean rust in soybean. Crop Sci, 2009, 49:265-271.
Hyten D L, Smith J R, Frederick R D, Tucker M L, Cregan P B. Bulked segregant analysis using the golden gate assay to locate the Rpp3 locus that confers resistance to soybean rust in soybean. Crop Sci, 2009, 49:265-271.
[28] Zou C, Wang P X, Xu Y B. Bulked sample analysis in genetics, genomics and crop improvement. Plant Biotechnol J, 2016, 14:1941-1955.
Zou C, Wang P X, Xu Y B. Bulked sample analysis in genetics, genomics and crop improvement. Plant Biotechnol J, 2016, 14:1941-1955.
[29] 王楚彪, 卢万鸿, 林彦, 罗建中. 转录组测序的发展和应用. 桉树科技, 2018, 35(4):20-26.
王楚彪, 卢万鸿, 林彦, 罗建中. 转录组测序的发展和应用. 桉树科技, 2018, 35(4):20-26.
Wang C B, Lu W H, Lin Y, Luo J Z. Development and application of transcriptome sequencing. Euct Sci Technol, 2018, 35(4):20-26 (in Chinese with English abstract).
Wang C B, Lu W H, Lin Y, Luo J Z. Development and application of transcriptome sequencing. Euct Sci Technol, 2018, 35(4):20-26 (in Chinese with English abstract).
[30] Gao J, Dai G X, Zhou W Y, Liang H F, Huang J, Qing D J, Chen W W, Wu H, Yang X H, Li D, Deng G F. Mapping and identifying a candidate gene Plr4, a recessive gene regulating purple leaf in rice, by using bulked segregant and transcriptome analysis with next-generation sequencing. Int J Mol Sci, 2019, 20:4335.
Gao J, Dai G X, Zhou W Y, Liang H F, Huang J, Qing D J, Chen W W, Wu H, Yang X H, Li D, Deng G F. Mapping and identifying a candidate gene Plr4, a recessive gene regulating purple leaf in rice, by using bulked segregant and transcriptome analysis with next-generation sequencing. Int J Mol Sci, 2019, 20:4335.
[31] Sunggil K, Cheol W K, Minkyu P, Doil C. Identification of candidate genes associated with fertility restoration of cytoplasmic male-sterility in onion (Allium cepa L.) using a combination of bulked segregant analysis and RNA-seq. Theor Appl Genet, 2015, 128:2289-2299.
Sunggil K, Cheol W K, Minkyu P, Doil C. Identification of candidate genes associated with fertility restoration of cytoplasmic male-sterility in onion (Allium cepa L.) using a combination of bulked segregant analysis and RNA-seq. Theor Appl Genet, 2015, 128:2289-2299.
[32] Li H, Bob H, Wysoker A, Tim F, Jue R, Nils H, Gabor M, Richard D. The sequence alignment/map format and SAMtools. bioinformatics. Bioinformatics, 2009, 25:2078-2079.
Li H, Bob H, Wysoker A, Tim F, Jue R, Nils H, Gabor M, Richard D. The sequence alignment/map format and SAMtools. bioinformatics. Bioinformatics, 2009, 25:2078-2079.
[33] McKenna A, Hanna M, Banks M, Sivachenko A, Cibulskis K, Kernytsky A, Garimella K, Altshuler D, Gabriel S, DePristo M A. The genome analysis toolkit: a map reduce framework for analyzing next-generation DNA sequencing data. Genome Res, 2010, 20:1297-1303.
McKenna A, Hanna M, Banks M, Sivachenko A, Cibulskis K, Kernytsky A, Garimella K, Altshuler D, Gabriel S, DePristo M A. The genome analysis toolkit: a map reduce framework for analyzing next-generation DNA sequencing data. Genome Res, 2010, 20:1297-1303.
[34] Trapnell C, Williams B A, Pertea G, Mortazavi A, Kwan G, Marijke J B, Salzberg S L, Lior P. Transcript assembly and quantification by RNA-Seq reveals unannotated transcripts and isoform switching during cell differentiation. Nat Biotechnol, 2010, 28:511-515.
Trapnell C, Williams B A, Pertea G, Mortazavi A, Kwan G, Marijke J B, Salzberg S L, Lior P. Transcript assembly and quantification by RNA-Seq reveals unannotated transcripts and isoform switching during cell differentiation. Nat Biotechnol, 2010, 28:511-515.
[35] Simon A, Paul P, Huber T, Wolf G. HTSeq: a Python framework to work with high-throughput sequencing data. Bioinformatics, 2015, 31:166-169.
Simon A, Paul P, Huber T, Wolf G. HTSeq: a Python framework to work with high-throughput sequencing data. Bioinformatics, 2015, 31:166-169.
[36] 苏倩, 吴洁, 杜佳慧, 杨凡, 袁濮玉, 刘松柏, 邱秀芹. 荧光定量PCR对FEN1敲低细胞株转录组测序结果的验证. 重庆医学, 2019, 48:2528-2531.
苏倩, 吴洁, 杜佳慧, 杨凡, 袁濮玉, 刘松柏, 邱秀芹. 荧光定量PCR对FEN1敲低细胞株转录组测序结果的验证. 重庆医学, 2019, 48:2528-2531.
Su Q, Wu J, Du J H, Yang F, Yuan P Y, Liu S B, Qiu X Q. Verification of transcriptome sequencing of FEN1 knockdown cell line by real-time quantitative PCR. Chongqing Med J, 2019, 48:2528-2531 (in Chinese with English abstract).
Su Q, Wu J, Du J H, Yang F, Yuan P Y, Liu S B, Qiu X Q. Verification of transcriptome sequencing of FEN1 knockdown cell line by real-time quantitative PCR. Chongqing Med J, 2019, 48:2528-2531 (in Chinese with English abstract).
[37] 张尧锋, 张冬青, 余华胜, 林宝刚, 华水金, 丁厚栋, 傅鹰. 基于极端混合池(BSA)全基因组重测序的甘蓝型油菜有限花序基因定位. 中国农业科学, 2018, 51:3029-3039.
张尧锋, 张冬青, 余华胜, 林宝刚, 华水金, 丁厚栋, 傅鹰. 基于极端混合池(BSA)全基因组重测序的甘蓝型油菜有限花序基因定位. 中国农业科学, 2018, 51:3029-3039.
Zhang Y F, Zhang D Q, Yu H S, Lin B G, Hua S J, Ding H D, Fu Y. Location and mapping of the determinate growth habit of Brassica napus by bulked segregant analysis (BSA) using whole genome re-sequencing. Sci Agric Sin, 2018, 51:3029-3039.
Zhang Y F, Zhang D Q, Yu H S, Lin B G, Hua S J, Ding H D, Fu Y. Location and mapping of the determinate growth habit of Brassica napus by bulked segregant analysis (BSA) using whole genome re-sequencing. Sci Agric Sin, 2018, 51:3029-3039.
[38] Kaur H, Banga S S. Discovery and mapping of Brassica juncea Sdt 1 gene associated with determinate plant growth habit. Theor Appl Genet, 2015, 128:235-245.
Kaur H, Banga S S. Discovery and mapping of Brassica juncea Sdt 1 gene associated with determinate plant growth habit. Theor Appl Genet, 2015, 128:235-245.
[39] Li K X, Yao Y M, Xiao L, Zhao Z G, Guo S M, Du D Z. Fine mapping of the Brassica napus Bnsdt1 gene associated with determinate growth habit. Theor Appl Genet, 2018, 131:193-208.
Li K X, Yao Y M, Xiao L, Zhao Z G, Guo S M, Du D Z. Fine mapping of the Brassica napus Bnsdt1 gene associated with determinate growth habit. Theor Appl Genet, 2018, 131:193-208.
[40] Shannon S. A mutation in the Arabidopsis TFL1 gene affects inflorescence meristem development. Plant Cell, 1991, 3:877-892.
Shannon S. A mutation in the Arabidopsis TFL1 gene affects inflorescence meristem development. Plant Cell, 1991, 3:877-892.
[41] Long J A, Ohno C, Smith Z R, Meyerowitz E M. TOPLESS regulates apical embryonic fate in Arabidopsis. Science, 2006, 312:1520-1523.
Long J A, Ohno C, Smith Z R, Meyerowitz E M. TOPLESS regulates apical embryonic fate in Arabidopsis. Science, 2006, 312:1520-1523.
[42] Krizek B A, Ivory C. Blakley, Ho Y Y, Loraine A E. The Arabidopsis transcription factor AINTEGUMENTA orchestrates patterning genes and auxin signaling in the establishment of floral growth and form. Plant J, 2020, 103:752-768.
Krizek B A, Ivory C. Blakley, Ho Y Y, Loraine A E. The Arabidopsis transcription factor AINTEGUMENTA orchestrates patterning genes and auxin signaling in the establishment of floral growth and form. Plant J, 2020, 103:752-768.
[43] 万薇, 余坤江, 叶波涛, Aimal N K, 王天娅, 杨仁芹, 林树春, 田恩堂. TFL1相关基因调控植物花序发育的分子机制. 植物生理学报, 2020, 56:367-372.
万薇, 余坤江, 叶波涛, Aimal N K, 王天娅, 杨仁芹, 林树春, 田恩堂. TFL1相关基因调控植物花序发育的分子机制. 植物生理学报, 2020, 56:367-372.
Wan W, Yu K J, Ye B T, Aimal N K, Wang T Y, Yang R Q, Lin S C, Tian E T. Molecular mechanism of TFL1 related genes regulating plant inflorescence development. J Plant Physiol, 2020, 56:367-372.
Wan W, Yu K J, Ye B T, Aimal N K, Wang T Y, Yang R Q, Lin S C, Tian E T. Molecular mechanism of TFL1 related genes regulating plant inflorescence development. J Plant Physiol, 2020, 56:367-372.
[44] Sriboon S, Li H T, Guo C C, Senkhamwong T, Cheng D, Liu K D. Knock-out of TERMINAL FLOWER 1 genes altered flowering time and plant architecture in Brassica napus. BMC Genet, 2020, 21:627-348.
Sriboon S, Li H T, Guo C C, Senkhamwong T, Cheng D, Liu K D. Knock-out of TERMINAL FLOWER 1 genes altered flowering time and plant architecture in Brassica napus. BMC Genet, 2020, 21:627-348.
[45] Ledger S, Strayer C, Ashton F, Kay S A, Putterill J. Analysis of the function of two circadian-regulated CONSTANS-LIKE genes. Plant J, 2001, 26:15-22
Ledger S, Strayer C, Ashton F, Kay S A, Putterill J. Analysis of the function of two circadian-regulated CONSTANS-LIKE genes. Plant J, 2001, 26:15-22
[46] Song H R, Song J D, Cho J N, Amasino R M, Noh Y S. The RNA binding protein ELF9 directly reduces suppressor of overexpression of CO1 transcript levels in Arabidopsis, possibly via nonsensemediated mRNA decay. Plant Cell, 2009, 21:1195-1211.
Song H R, Song J D, Cho J N, Amasino R M, Noh Y S. The RNA binding protein ELF9 directly reduces suppressor of overexpression of CO1 transcript levels in Arabidopsis, possibly via nonsensemediated mRNA decay. Plant Cell, 2009, 21:1195-1211.
[47] Wiszniewski A A G, Bussell J D, Smith S M. Knockout of the two evolutionarily conserved peroxisomal 3-ketoacyl-CoA thiolases in Arabidopsis recapitulates the abnormal inflorescence meristem 1 phenotype. J Exp Bot, 2014, 65:6723-6733.
Wiszniewski A A G, Bussell J D, Smith S M. Knockout of the two evolutionarily conserved peroxisomal 3-ketoacyl-CoA thiolases in Arabidopsis recapitulates the abnormal inflorescence meristem 1 phenotype. J Exp Bot, 2014, 65:6723-6733.
[48] Tan L M, Liu R, Gu B W, Zhang C J, Luo J Y, Guo J, Wang Y H, Chen L X, Du X, Li S S, Shao C R, Su Y N, Cai X W, Lin R N, Li L, Chen S, He X J. Dual recognition of H3K4me3 and DNA by the ISWI component ARID5 regulates the floral transition inArabidopsis. Plant Cell, 2020, 32:2178-2195.
Tan L M, Liu R, Gu B W, Zhang C J, Luo J Y, Guo J, Wang Y H, Chen L X, Du X, Li S S, Shao C R, Su Y N, Cai X W, Lin R N, Li L, Chen S, He X J. Dual recognition of H3K4me3 and DNA by the ISWI component ARID5 regulates the floral transition inArabidopsis. Plant Cell, 2020, 32:2178-2195.
[1] 陈松余, 丁一娟, 孙峻溟, 黄登文, 杨楠, 代雨涵, 万华方, 钱伟. 甘蓝型油菜BnCNGC基因家族鉴定及其在核盘菌侵染和PEG处理下的表达特性分析[J]. 作物学报, 2022, 48(6): 1357-1371.
[2] 田甜, 陈丽娟, 何华勤. 基于Meta-QTL和RNA-seq的整合分析挖掘水稻抗稻瘟病候选基因[J]. 作物学报, 2022, 48(6): 1372-1388.
[3] 秦璐, 韩配配, 常海滨, 顾炽明, 黄威, 李银水, 廖祥生, 谢立华, 廖星. 甘蓝型油菜耐低氮种质筛选及绿肥应用潜力评价[J]. 作物学报, 2022, 48(6): 1488-1501.
[4] 李阿立, 冯雅楠, 李萍, 张东升, 宗毓铮, 林文, 郝兴宇. 大豆叶片响应CO2浓度升高、干旱及其交互作用的转录组分析[J]. 作物学报, 2022, 48(5): 1103-1118.
[5] 黄伟, 高国应, 吴金锋, 刘丽莉, 张大为, 周定港, 成洪涛, 张凯旋, 周美亮, 李莓, 严明理. 芥菜型油菜BjA09.TT8BjB08.TT8基因调节类黄酮的合成[J]. 作物学报, 2022, 48(5): 1169-1180.
[6] 雷新慧, 万晨茜, 陶金才, 冷佳俊, 吴怡欣, 王家乐, 王鹏科, 杨清华, 冯佰利, 高金锋. 褪黑素与2,4-表油菜素内酯浸种对盐胁迫下荞麦发芽与幼苗生长的促进效应[J]. 作物学报, 2022, 48(5): 1210-1221.
[7] 石育钦, 孙梦丹, 陈帆, 成洪涛, 胡学志, 付丽, 胡琼, 梅德圣, 李超. 通过CRISPR/Cas9技术突变BnMLO6基因提高甘蓝型油菜的抗病性[J]. 作物学报, 2022, 48(4): 801-811.
[8] 袁大双, 邓琬玉, 王珍, 彭茜, 张晓莉, 姚梦楠, 缪文杰, 朱冬鸣, 李加纳, 梁颖. 甘蓝型油菜BnMAPK2基因的克隆及功能分析[J]. 作物学报, 2022, 48(4): 840-850.
[9] 黄成, 梁晓梅, 戴成, 文静, 易斌, 涂金星, 沈金雄, 傅廷栋, 马朝芝. 甘蓝型油菜BnAPs基因家族成员全基因组鉴定及分析[J]. 作物学报, 2022, 48(3): 597-607.
[10] 王瑞, 陈雪, 郭青青, 周蓉, 陈蕾, 李加纳. 甘蓝型油菜白花基因InDel连锁标记开发[J]. 作物学报, 2022, 48(3): 759-769.
[11] 娄洪祥, 姬建利, 蒯婕, 汪波, 徐亮, 李真, 刘芳, 黄威, 刘暑艳, 尹羽丰, 王晶, 周广生. 种植密度对油菜正反交组合产量与倒伏相关性状的影响[J]. 作物学报, 2021, 47(9): 1724-1740.
[12] 张建, 谢田晋, 尉晓楠, 王宗铠, 刘崇涛, 周广生, 汪波. 无人机多角度成像方式的饲料油菜生物量估算研究[J]. 作物学报, 2021, 47(9): 1816-1823.
[13] 王艳花, 刘景森, 李加纳. 整合GWAS和WGCNA筛选鉴定甘蓝型油菜生物产量候选基因[J]. 作物学报, 2021, 47(8): 1491-1510.
[14] 曾维英, 赖振光, 孙祖东, 杨守臻, 陈怀珠, 唐向民. 基于BSA-Seq和RNA-Seq方法鉴定大豆抗豆卷叶螟候选基因[J]. 作物学报, 2021, 47(8): 1460-1471.
[15] 李杰华, 端群, 史明涛, 吴潞梅, 柳寒, 林拥军, 吴高兵, 范楚川, 周永明. 新型抗广谱性除草剂草甘膦转基因油菜的创制及其鉴定[J]. 作物学报, 2021, 47(5): 789-798.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!