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作物学报

作物学报 ›› 2022, Vol. 48 ›› Issue (1): 76-85.doi: 10.3724/SP.J.1006.2022.04241

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

马铃薯StDRO1基因的多态性鉴定及其与根系性状的关联分析

许德蓉1,2(), 孙超1,2(), 毕真真1,2, 秦天元1,2, 王一好1,2, 李成举1,2, 范又方1,2, 刘寅笃1,2, 张俊莲1, 白江平1,2,*()   

  1. 1甘肃省干旱生境作物学重点实验室 / 甘肃省作物遗传改良与种质创新重点实验室, 甘肃兰州 730070
    2甘肃农业大学农学院, 甘肃兰州 730070
  • 收稿日期:2020-11-08 接受日期:2021-04-14 出版日期:2022-01-12 网络出版日期:2021-06-16
  • 通讯作者: 白江平
  • 作者简介:许德蓉, E-mail: 1830204585@qq.com
    孙超, E-mail: sunc@gsau.edu.cn第一联系人：**同等贡献
  • 基金资助:
    国家自然科学基金项目(32060502);国家自然科学基金项目(31660432);甘肃省创新能力提升项目(2019B-073);国家现代农业产业技术体系建设专项(马铃薯)(CARS-09-P14);甘肃省马铃薯产业体系建设专项(GARS-03-P1);甘肃省科技计划项目(19ZD2WA002-02);甘肃省科技计划项目(18JR3RA174);甘肃农业大学人才专项资助(2017RCZX-44)

Identification of StDRO1 gene polymorphism and association analysis with root traits in potato

XU De-Rong1,2(), SUN Chao1,2(), BI Zhen-Zhen1,2, QIN Tian-Yuan1,2, WANG Yi-Hao1,2, LI Cheng-Ju1,2, FAN You-Fang1,2, LIU Yin-Du1,2, ZHANG Jun-Lian1, BAI Jiang-Ping1,2,*()   

  1. 1Gansu Provincial Key Laboratory of Aridland Crop Science / Gansu Key Laboratory of Crop Improvement & Germplasm Enhancement, Lanzhou 730070, Gansu, China
    2College of Agronomy, Gansu Agricultural University, Lanzhou 730070, Gansu, China
  • Received:2020-11-08 Accepted:2021-04-14 Published:2022-01-12 Published online:2021-06-16
  • Contact: BAI Jiang-Ping
  • About author:First author contact:** Contributed equally to this work
  • Supported by:
    National Natural Science Foundation of China(32060502);National Natural Science Foundation of China(31660432);Gansu Provincial Education Department(2019B-073);China Agriculture Research System (Potato)(CARS-09-P14);Agriculture Research System of Gansu Province (Potato)(GARS-03-P1);Gansu Provincial Science and Technology Department Program(19ZD2WA002-02);Gansu Provincial Science and Technology Department Program(18JR3RA174);Fund of Gansu Agricultural University(2017RCZX-44)

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摘要：

为鉴定与马铃薯根系性状相关联的StDRO1基因单核苷酸多态性(single nucleotide polymorphism, SNP)位点, 本研究对110份同源四倍体马铃薯材料StDRO1基因的编码区进行了克隆和测序, 筛选其SNP, 并对StDRO1的SNP位点与马铃薯总根表面积、总根体积和平均根系直径等主要根系性状参数进行了关联分析。结果显示, StDRO1第2外显子上检测到1个SNP位点, 命名为G64C; 第3外显子上检测到10个SNP位点, 分别命名为G152A、A214G、A297G、C314T、A337T、T353C、T560A、C577A、C620A和C625A; 在第4外显子上检测到1个SNP位点, 命名为T793A。关联分析结果表明, StDRO1基因G152A位点在总根体积表现为GA类基因型>GG类基因型(P<0.05); C314T位点在平均根系直径表现为CC类基因型>CT类基因型(P<0.05); A337T位点在总根表面积、鲜重和干重均表现为AT类基因型>AA类基因型(P<0.05), 而在平均根系直径则表现为AA类基因型>AT类基因型(P<0.05); T353C位点在总根表面积、总根体积和鲜重均表现为TC类基因型>TT类基因型(P<0.05); C620A位点在总根体积表现为CA类基因型>CC类基因型(P<0.05); T793A位点在总根表面积、总根体积和鲜重均表现为AT类基因型>TT类基因型(P<0.05)。综上, StDRO1基因的上述6个SNP对马铃薯根系性状有显著影响, 其中A337T、T353C和T793A位点尤为重要。研究结果为后续马铃薯根系构型研究和遗传改良提供了理论参考, 但能否作为马铃薯根系性状遗传标记还需扩大群体样本进一步验证。

关键词: 马铃薯, StDRO1基因, 根系性状, 关联分析, SNP

Abstract:

In order to identify the single nucleotide polymorphism (SNP) of StDRO1 gene associated with potato root traits, StDRO1 gene coding region of the 110 tetraploid potato genotypes was cloned and sequenced. The association analysis between the StDRO1 SNPs and the major root traits of potato such as surface area, volume, and average diameter were carried out. The results showed that a SNP (G64C) was detected in the second exon of the StDRO1, 10 SNPs (G152A, A214G, A297G, C314T, A337T, T353C, T560A, C577A, C620A, and C625A) were identified in the third exon, and a SNP (T793A) was detected in the fourth exon. The association analysis indicated that in the total root volume, G152A locus of StDRO1 gene showed that GA genotype was superior to GG genotype (P < 0.05). In the average root diameter, C314 locus showed that CC genotype were superior to CT genotype (P < 0.05). In the root surface area, fresh weight, and dry weight, A337T locus showed that AT genotype were better than AA genotype (P < 0.05), while the locus in the average root diameter showed that AT genotype was significantly lower than AA genotype (P < 0.05). The root surface area, root volume, and fresh weight were showed as TC genotype > TT genotype (P < 0.05) in T353C site; the root volume was showed as CA genotype > CC genotype (P < 0.05) in C620A site; the root surface area, root volume, and fresh weight were showed as AT genotype > TT genotype (P < 0.05) in T793A site. In summary, the above six SNPs of StDRO1 genes had a significant impact on potato root traits, and the A337T, T353C, and T793A sites were particularly important. These results provide a basic theoretical reference for subsequent potato root architecture research and genetic improvement, but whether it could be used as a genetic marker for potato root traits need to be further verified by expanding the population sample.

Key words: potato, StDRO1 gene, root traits, association analysis, SNP

表1

110份马铃薯材料信息表"

材料编号
Material code		 CIP号
CIP code		 材料编号
Material code		 CIP号
CIP code		 材料编号
Material code		 CIP号
CIP code
C2 CIP391065.69 C56 CIP394034.65 C99 CIP388615.22
C4 CIP392617.54 C57 CIP394034.7 C100 CIP389468.3
C5 CIP392634.52 C58 CIP394579.36 C101 CIP390637.1
C8 CIP393227.66 C59 CIP394600.52 C102 CIP391180.6
C9 CIP393228.67 C60 CIP394611.112 C103 CIP391533.1
C10 CIP393371.164 C61 CIP394613.139 C104 CIP391724.1
C11 CIP391004.18 C62 CIP394613.32 C105 CIP392032.2
C13 CIP393280.64 C63 CIP394614.117 C106 CIP392740.4
C14 CIP391047.34 C64 CIP394881.8 C107 CIP392745.7
C15 CIP391058.175 C65 CIP395186.6 C108 CIP392759.1
C16 CIP393085.5 C66 CIP395193.6 C109 CIP393613.2
C17 CIP398192.213 C67 CIP395195.7 C110 CIP393615.6
C18 CIP398098.119 C68 CIP395196.4 C112 CIP397030.31
C19 CIP398098.203 C70 CIP395432.51 C113 CIP397035.26
C20 CIP398180.253 C71 CIP395434.1 C114 CIP302428.20
C21 CIP398180.289 C72 CIP395436.8 C115 CIP302476.108
C23 CIP398180.612 C74 CIP396311.1 C116 CIP302499.30
C27 CIP398208.33 C79 CIP397029.21 C117 CIP304345.102
C28 CIP398208.58 C80 CIP397036.7 C119 CIP304350.118
C29 CIP398208.704 C81 CIP397039.51 C120 CIP304350.95
C30 CIP301024.14 C82 CIP397044.25 C121 CIP304371.67
C32 CIP301040.63 C83 CIP397055.2 C122 CIP304383.41
C33 CIP300046.22 C84 CIP397065.2 C123 CIP304383.80
C34 CIP300048.12 C85 CIP397067.2 C124 CIP304387.39
C35 CIP300054.29 C86 CIP397069.5 C127 CIP397077.16
C39 CIP300072.1 C87 CIP397073.15 C128 CIP391919.3
C41 CIP300099.22 C88 CIP397078.12 C130 CIP391931.1
C43 CIP379706.27 C89 CIP397079.26 C131 CIP394906.6
C44 CIP385499.11 C90 CIP397079.6 C132 CIP395438.1
C46 CIP388676.1 C91 CIP397098.12 C133 CIP394904.20
C48 CIP390478.9 C92 CIP397099.6 Atl 大西洋Atlantic
C49 CIP391207.2 C93 CIP397100.9 QS9 青薯9号Qingshu 9
C50 CIP391382.18 C94 CIP397196.3 GN2 甘农薯2号Gannongshu 2
C51 CIP392781.1 C95 CIP397196.8 LS3 陇薯3号Longshu 3
C52 CIP392797.22 C96 CIP397197.9 LS6 陇薯6号Longshu 6
C53 CIP392822.3 C97 CIP398014.2 Fa 费乌瑞它Favorita
C54 CIP392973.48 C98 CIP388611.22

表2

PCR引物序列"

引物
Primer		 引物序列
Primer sequences (5′-3′)		 退火温度
Annealing temperature (℃)		 产物片段长度
Product fragment length (bp)
DRO1-F CCATTCAAACATCATCACAAGA 55 1670
DRO1-R GAAATGGAACAACTAGCAGAGA

图1

StDRO1基因PCR产物电泳检测结果 M为DNA 2000分子标记; 1、2、3、4和5分别表示材料C2、C4、C5、C8和C9的StDRO1扩增片段。"

图1

StDRO1基因PCR产物电泳检测结果 M为DNA 2000分子标记; 1、2、3、4和5分别表示材料C2、C4、C5、C8和C9的StDRO1扩增片段。"

图2

马铃薯StDRO1基因序列比对结果"

图2

马铃薯StDRO1基因序列比对结果"

表3

不同马铃薯材料StDRO1基因编码区的单核苷酸变异"

碱基类型
Base type		 碱基位置Base site (bp)
64 152 214 297 314 337 353 560 577 620 625 793
参考基因组碱基类型
Base type of reference genome		 G G A A C A T T C C C T
变异碱基类型
Variant base type		 C A G G T T C A A A A A

表3

不同马铃薯材料StDRO1基因编码区的单核苷酸变异"

碱基类型
Base type		 碱基位置Base site (bp)
64 152 214 297 314 337 353 560 577 620 625 793
参考基因组碱基类型
Base type of reference genome		 G G A A C A T T C C C T
变异碱基类型
Variant base type		 C A G G T T C A A A A A

表4

马铃薯StDRO1的基因型频率和基因频率"

单核苷酸多态性位点
SNP		 样本数
Sample number		 基因型频率
Genotype frequency		 基因频率
Gene frequency		 卡平方
χ2
G64C 110 GG
0.2818 (31)		 GC
0.7182 (79)		 G
0.6409		 C
0.3591		 34.5310
G152A 110 GG
0.8364 (92)		 GA
0.1636 (18)		 G
0.9182		 A
0.0818		 0.8734
A214G 110 AA
0.3909 (43)		 AG
0.6091 (67)		 A
0.6955		 G
0.3045		 21.0940
A297G 110 AA
0.2545 (28)		 AG
0.7455 (82)		 A
0.6273		 G
0.3727		 38.8385
C314T 110 CC
0.6818 (75)		 CT
0.3182 (35)		 C
0.8409		 T
0.1591		 3.9372
单核苷酸多态性位点
SNP		 样本数
Sample number		 基因型频率
Genotype frequency		 基因频率
Gene frequency		 卡平方
χ2
A337T 110 AA
0.6818 (75)		 AT
0.3182 (35)		 A
0.8409		 T
0.1591		 3.9372
T353C 110 TT
0.8182 (90)		 TC
0.1818 (20)		 T
0.9091		 C
0.0909		 1.1000
T560A 110 AA
0.6182 (68)		 AT
0.3818 (42)		 A
0.8091		 T
0.1909		 6.1242
C577A 110 CC
0.2818 (31)		 CA
0.7182 (79)		 C
0.6409		 A
0.3591		 34.5310
C620A 110 CC
0.3545 (39)		 CA
0.6455 (71)		 C
0.6773		 A
0.3227		 24.9768
C625A 110 CC
0.6545 (72)		 CA
0.3455 (38)		 C
0.8273		 A
0.1727		 4.7953
T793A 110 AT
0.7909 (87)		 TT
0.2091 (23)		 A
0.3955		 T
0.6045		 47.0682

表4

马铃薯StDRO1的基因型频率和基因频率"

单核苷酸多态性位点
SNP		 样本数
Sample number		 基因型频率
Genotype frequency		 基因频率
Gene frequency		 卡平方
χ2
G64C 110 GG
0.2818 (31)		 GC
0.7182 (79)		 G
0.6409		 C
0.3591		 34.5310
G152A 110 GG
0.8364 (92)		 GA
0.1636 (18)		 G
0.9182		 A
0.0818		 0.8734
A214G 110 AA
0.3909 (43)		 AG
0.6091 (67)		 A
0.6955		 G
0.3045		 21.0940
A297G 110 AA
0.2545 (28)		 AG
0.7455 (82)		 A
0.6273		 G
0.3727		 38.8385
C314T 110 CC
0.6818 (75)		 CT
0.3182 (35)		 C
0.8409		 T
0.1591		 3.9372
单核苷酸多态性位点
SNP		 样本数
Sample number		 基因型频率
Genotype frequency		 基因频率
Gene frequency		 卡平方
χ2
A337T 110 AA
0.6818 (75)		 AT
0.3182 (35)		 A
0.8409		 T
0.1591		 3.9372
T353C 110 TT
0.8182 (90)		 TC
0.1818 (20)		 T
0.9091		 C
0.0909		 1.1000
T560A 110 AA
0.6182 (68)		 AT
0.3818 (42)		 A
0.8091		 T
0.1909		 6.1242
C577A 110 CC
0.2818 (31)		 CA
0.7182 (79)		 C
0.6409		 A
0.3591		 34.5310
C620A 110 CC
0.3545 (39)		 CA
0.6455 (71)		 C
0.6773		 A
0.3227		 24.9768
C625A 110 CC
0.6545 (72)		 CA
0.3455 (38)		 C
0.8273		 A
0.1727		 4.7953
T793A 110 AT
0.7909 (87)		 TT
0.2091 (23)		 A
0.3955		 T
0.6045		 47.0682

表5

马铃薯StDRO1基因群体遗传特异性"

单核苷酸多态性位点SNP 纯合度
Homozygosity		 杂合度Heterozygosity 有效等位基因数
Effective number of alleles		 多态信息含量
Polymorphism information content (PIC)
G64C 0.5397 0.4603 1.8528 0.3544
G152A 0.8498 0.1502 1.1768 0.1390
A214G 0.5764 0.4236 1.7349 0.3339
A297G 0.5324 0.4676 1.8783 0.3583
C314T 0.7324 0.2676 1.3653 0.2318
A337T 0.7324 0.2676 1.3653 0.2318
T353C 0.8347 0.1653 1.1980 0.1516
A560T 0.6911 0.3089 1.4470 0.2612
C577A 0.5397 0.4603 1.8528 0.3544
C620A 0.5629 0.4371 1.7767 0.3416
C625A 0.7142 0.2858 1.4001 0.2449
A793T 0.5219 0.4781 1.9162 0.3638

表5

马铃薯StDRO1基因群体遗传特异性"

单核苷酸多态性位点SNP 纯合度
Homozygosity		 杂合度Heterozygosity 有效等位基因数
Effective number of alleles		 多态信息含量
Polymorphism information content (PIC)
G64C 0.5397 0.4603 1.8528 0.3544
G152A 0.8498 0.1502 1.1768 0.1390
A214G 0.5764 0.4236 1.7349 0.3339
A297G 0.5324 0.4676 1.8783 0.3583
C314T 0.7324 0.2676 1.3653 0.2318
A337T 0.7324 0.2676 1.3653 0.2318
T353C 0.8347 0.1653 1.1980 0.1516
A560T 0.6911 0.3089 1.4470 0.2612
C577A 0.5397 0.4603 1.8528 0.3544
C620A 0.5629 0.4371 1.7767 0.3416
C625A 0.7142 0.2858 1.4001 0.2449
A793T 0.5219 0.4781 1.9162 0.3638

表6

StDRO1基因多态性与马铃薯根系性状的关联分析"

单核苷酸多态性位点SNP 基因型Genotype 总根表面积
Surface area (cm2)		 平均根系直径
Average diameter (mm)		 总根体积
Root volume (cm3)		 鲜重
Fresh weight (g)		 干重
Dry weight (g)
G64C GG 14.9413±3.1468 a 0.4150±0.0604 a 0.1412±0.0495 a 0.1393±0.0649 a 0.0107±0.0047 a
GC 14.0160±3.7477 a 0.4270±0.1101 a 0.1482±0.0577 a 0.1352±0.0568 a 0.0100±0.0053 a
G152A GG 13.9844±3.5567 a 0.4237±0.1040 a 0.1415±0.0529 b 0.1335±0.0571 a 0.0102±0.0052 a
GA 15.7710±3.5351 a 0.4233±0.0660 a 0.1702±0.0629 a 0.1514±0.0672 a 0.0102±0.0048 a
A214G AA 13.5207±3.4322 a 0.4401±0.1031 a 0.1509±0.0590 a 0.1283±0.0586 a 0.0094±0.0053 a
AG 14.7620±3.6443 a 0.4131±0.0948 a 0.1432±0.0531 a 0.1416±0.0590 a 0.0106±0.0049 a
A297G AA 14.5685±3.2391 a 0.4056±0.0572 a 0.1340±0.0462 a 0.1400±0.0635 a 0.0107±0.0051 a
AG 14.1772±3.7271 a 0.4298±0.1087 a 0.1504±0.0578 a 0.1351±0.0576 a 0.0100±0.0051 a
C314T CC 13.9384±3.4374 a 0.4378±0.1073 a 0.1475±0.0578 a 0.1302±0.0573 a 0.0096±0.0051 a
CT 15.0018±3.8743 a 0.3934±0.0683 b 0.1434±0.0503 a 0.1498±0.0609 a 0.0114±0.0050 a
A337T AA 13.7373±3.4046 b 0.4372±0.1061 a 0.1465±0.0593 a 0.1282±0.0553 b 0.0094±0.0048 b
AT 15.4327±3.7782 a 0.3946±0.0731 b 0.1455±0.0466 a 0.1541±0.0633 a 0.0118±0.0053 a
单核苷酸多态性位点SNP 基因型Genotype 总根表面积
Surface area (cm2)		 平均根系直径
Average diameter (mm)		 总根体积
Root volume (cm3)		 鲜重
Fresh weight (g)		 干重
Dry weight (g)
T353C TT 13.9052±3.5271 b 0.4238±0.1050 a 0.1407±0.0527 b 0.1312±0.0555 b 0.0100±0.0051 a
TC 15.9487±3.5253 a 0.4229±0.0639 a 0.1712±0.0613 a 0.1597±0.0691 a 0.0109±0.0052 a
T560A AA 13.7829±3.3268 a 0.4295±0.1115 a 0.1381±0.0521 a 0.1303±0.0545 a 0.0100±0.0051 a
AT 15.0764±3.9096 a 0.4142±0.0732 a 0.1593±0.0585 a 0.1462±0.0649 a 0.0104±0.0051 a
C577A CC 14.5161±3.7664 a 0.4038±0.0608 a 0.1363±0.0477 a 0.1432±0.0617 a 0.0109±0.0049 a
CA 14.1828±3.5511 a 0.4314±0.1092 a 0.1501±0.0579 a 0.1337±0.0580 a 0.0099±0.0052 a
C620A CC 14.0477±3.6818 a 0.4018±0.0589 a 0.1307±0.0449 b 0.1407±0.0627 a 0.0103±0.0050 a
CA 14.4026±3.5725 a 0.4356±0.1132 a 0.1548±0.0589 a 0.1340±0.0570 a 0.0101±0.0052 a
C625A CC 14.3825±3.5532 a 0.4159±0.0926 a 0.1450±0.0551 a 0.1334±0.0552 a 0.0099±0.0051 a
CA 14.0765±3.7234 a 0.4384±0.1087 a 0.1485±0.0565 a 0.1420±0.0658 a 0.0106±0.0052 a
T793A TT 12.4573±2.7287 b 0.4114±0.0964 a 0.1259±0.0515 b 0.1134±0.0482 b 0.0086±0.0041 a
AT 14.7578±3.6593 a 0.4269±0.0994 a 0.1516±0.0554 a 0.1425±0.0602 a 0.0106±0.0053 a

表6

StDRO1基因多态性与马铃薯根系性状的关联分析"

单核苷酸多态性位点SNP 基因型Genotype 总根表面积
Surface area (cm2)		 平均根系直径
Average diameter (mm)		 总根体积
Root volume (cm3)		 鲜重
Fresh weight (g)		 干重
Dry weight (g)
G64C GG 14.9413±3.1468 a 0.4150±0.0604 a 0.1412±0.0495 a 0.1393±0.0649 a 0.0107±0.0047 a
GC 14.0160±3.7477 a 0.4270±0.1101 a 0.1482±0.0577 a 0.1352±0.0568 a 0.0100±0.0053 a
G152A GG 13.9844±3.5567 a 0.4237±0.1040 a 0.1415±0.0529 b 0.1335±0.0571 a 0.0102±0.0052 a
GA 15.7710±3.5351 a 0.4233±0.0660 a 0.1702±0.0629 a 0.1514±0.0672 a 0.0102±0.0048 a
A214G AA 13.5207±3.4322 a 0.4401±0.1031 a 0.1509±0.0590 a 0.1283±0.0586 a 0.0094±0.0053 a
AG 14.7620±3.6443 a 0.4131±0.0948 a 0.1432±0.0531 a 0.1416±0.0590 a 0.0106±0.0049 a
A297G AA 14.5685±3.2391 a 0.4056±0.0572 a 0.1340±0.0462 a 0.1400±0.0635 a 0.0107±0.0051 a
AG 14.1772±3.7271 a 0.4298±0.1087 a 0.1504±0.0578 a 0.1351±0.0576 a 0.0100±0.0051 a
C314T CC 13.9384±3.4374 a 0.4378±0.1073 a 0.1475±0.0578 a 0.1302±0.0573 a 0.0096±0.0051 a
CT 15.0018±3.8743 a 0.3934±0.0683 b 0.1434±0.0503 a 0.1498±0.0609 a 0.0114±0.0050 a
A337T AA 13.7373±3.4046 b 0.4372±0.1061 a 0.1465±0.0593 a 0.1282±0.0553 b 0.0094±0.0048 b
AT 15.4327±3.7782 a 0.3946±0.0731 b 0.1455±0.0466 a 0.1541±0.0633 a 0.0118±0.0053 a
单核苷酸多态性位点SNP 基因型Genotype 总根表面积
Surface area (cm2)		 平均根系直径
Average diameter (mm)		 总根体积
Root volume (cm3)		 鲜重
Fresh weight (g)		 干重
Dry weight (g)
T353C TT 13.9052±3.5271 b 0.4238±0.1050 a 0.1407±0.0527 b 0.1312±0.0555 b 0.0100±0.0051 a
TC 15.9487±3.5253 a 0.4229±0.0639 a 0.1712±0.0613 a 0.1597±0.0691 a 0.0109±0.0052 a
T560A AA 13.7829±3.3268 a 0.4295±0.1115 a 0.1381±0.0521 a 0.1303±0.0545 a 0.0100±0.0051 a
AT 15.0764±3.9096 a 0.4142±0.0732 a 0.1593±0.0585 a 0.1462±0.0649 a 0.0104±0.0051 a
C577A CC 14.5161±3.7664 a 0.4038±0.0608 a 0.1363±0.0477 a 0.1432±0.0617 a 0.0109±0.0049 a
CA 14.1828±3.5511 a 0.4314±0.1092 a 0.1501±0.0579 a 0.1337±0.0580 a 0.0099±0.0052 a
C620A CC 14.0477±3.6818 a 0.4018±0.0589 a 0.1307±0.0449 b 0.1407±0.0627 a 0.0103±0.0050 a
CA 14.4026±3.5725 a 0.4356±0.1132 a 0.1548±0.0589 a 0.1340±0.0570 a 0.0101±0.0052 a
C625A CC 14.3825±3.5532 a 0.4159±0.0926 a 0.1450±0.0551 a 0.1334±0.0552 a 0.0099±0.0051 a
CA 14.0765±3.7234 a 0.4384±0.1087 a 0.1485±0.0565 a 0.1420±0.0658 a 0.0106±0.0052 a
T793A TT 12.4573±2.7287 b 0.4114±0.0964 a 0.1259±0.0515 b 0.1134±0.0482 b 0.0086±0.0041 a
AT 14.7578±3.6593 a 0.4269±0.0994 a 0.1516±0.0554 a 0.1425±0.0602 a 0.0106±0.0053 a

表7

不同SNP位点组合与马铃薯总根表面积和鲜重的关联分析"

基因型组合编号
Code of genotype combinations		 基因型组合
Genotype combinations		 总根表面积
Surface area (cm2)		 鲜重
Fresh weight (g)
7-1 337AA+353TT+793AT 13.7148±3.3564 ab 0.1290±0.0530 ab
7-2 337AA+353TT+793TT 12.0009±2.2114 b 0.1028±0.0364 b
7-3 337AA+353TC+793AT 16.0900±3.6150 a 0.1532±0.0674 ab
7-4 337AA+353TC+793TT 14.7243±3.3069 ab 0.1583±0.0783 ab
7-5 337AT+353TT+793AT 15.3848±3.8763 ab 0.1515±0.0621 ab
7-6 337AT+353TT+793TT 11.6084±2.0201 b 0.1250±0.0230 ab
7-7 337AT+353TC+793AT 16.7491±3.0515 a 0.1808±0.0621 a

表7

不同SNP位点组合与马铃薯总根表面积和鲜重的关联分析"

基因型组合编号
Code of genotype combinations		 基因型组合
Genotype combinations		 总根表面积
Surface area (cm2)		 鲜重
Fresh weight (g)
7-1 337AA+353TT+793AT 13.7148±3.3564 ab 0.1290±0.0530 ab
7-2 337AA+353TT+793TT 12.0009±2.2114 b 0.1028±0.0364 b
7-3 337AA+353TC+793AT 16.0900±3.6150 a 0.1532±0.0674 ab
7-4 337AA+353TC+793TT 14.7243±3.3069 ab 0.1583±0.0783 ab
7-5 337AT+353TT+793AT 15.3848±3.8763 ab 0.1515±0.0621 ab
7-6 337AT+353TT+793TT 11.6084±2.0201 b 0.1250±0.0230 ab
7-7 337AT+353TC+793AT 16.7491±3.0515 a 0.1808±0.0621 a

表8

不同SNP位点组合与马铃薯平均根系直径的关联分析"

基因型组合编号
Code of genotype combinations		 基因型组合
Genotype combinations		 平均根系直径
Average diameter (mm)
8-1 314CC+337AA 0.4420±0.1073 a
8-2 314CC+337AT 0.3794±0.0992 a
8-3 314CT+337AA 0.3708±0.0600 a
8-4 314CT+337AT 0.3971±0.0698 a

表8

不同SNP位点组合与马铃薯平均根系直径的关联分析"

基因型组合编号
Code of genotype combinations		 基因型组合
Genotype combinations		 平均根系直径
Average diameter (mm)
8-1 314CC+337AA 0.4420±0.1073 a
8-2 314CC+337AT 0.3794±0.0992 a
8-3 314CT+337AA 0.3708±0.0600 a
8-4 314CT+337AT 0.3971±0.0698 a

表9

不同SNP位点组合与马铃薯总根体积的关联分析"

基因型组合编号
Code of genotype combinations		 基因型组合
Genotype combinations		 总根体积
Root volume (cm3)
9-1 152GG+353TT+620CC+793AT 0.1399±0.0441 bc
9-2 152GG+353TT+620CC+793TT 0.0967±0.0229 c
9-3 152GG+353TT+620CA+793AT 0.1500±0.0558 bc
9-4 152GG+353TT+620CA+793TT 0.1344±0.0561 bc
9-5 152GG+353TC+620CA+793AT 0.1343±0.0098 bc
9-6 152GG+353TC+620CA+793TT 0.2247±0.0198 a
9-7 152GA+353TC+620CC+793AT 0.1347±0.0494 bc
9-8 152GA+353TC+620CC+793TT 0.1754±0.0511 ab
9-9 152GA+353TC+620CA+793AT 0.2186±0.0543 a
9-10 152GA+353TC+620CA+793TT 0.1053±0.0553 bc

表9

不同SNP位点组合与马铃薯总根体积的关联分析"

基因型组合编号
Code of genotype combinations		 基因型组合
Genotype combinations		 总根体积
Root volume (cm3)
9-1 152GG+353TT+620CC+793AT 0.1399±0.0441 bc
9-2 152GG+353TT+620CC+793TT 0.0967±0.0229 c
9-3 152GG+353TT+620CA+793AT 0.1500±0.0558 bc
9-4 152GG+353TT+620CA+793TT 0.1344±0.0561 bc
9-5 152GG+353TC+620CA+793AT 0.1343±0.0098 bc
9-6 152GG+353TC+620CA+793TT 0.2247±0.0198 a
9-7 152GA+353TC+620CC+793AT 0.1347±0.0494 bc
9-8 152GA+353TC+620CC+793TT 0.1754±0.0511 ab
9-9 152GA+353TC+620CA+793AT 0.2186±0.0543 a
9-10 152GA+353TC+620CA+793TT 0.1053±0.0553 bc
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