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作物学报 ›› 2023, Vol. 49 ›› Issue (10): 2698-2704.doi: 10.3724/SP.J.1006.2023.24251

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

花生蔗糖含量与蛋白质和含油量的相关性分析及蔗糖含量QTL定位

郭建斌1(), 成良强2(), 李威涛1, 刘念1, 罗怀勇1, 丁膺宾1, 喻博伦1, 陈伟刚1, 黄莉1, 周小静1, 雷永1, 廖伯寿1, 姜慧芳1()   

  1. 1中国农业科学院油料作物研究所 / 农业农村部油料作物生物学与遗传育种重点实验室, 湖北武汉 430062
    2贵州省农业科学院油料研究所, 贵州贵阳 550006
  • 收稿日期:2022-11-11 接受日期:2023-02-21 出版日期:2023-10-12 网络出版日期:2023-03-01
  • 通讯作者: 姜慧芳, E-mail: peanutlab@oilcrops.cn
  • 作者简介:郭建斌, E-mail: guojianbin1990@163.com;成良强, E-mail: chengliangmei1984@126.com **同等贡献
  • 基金资助:
    贵州省科技计划项目(黔科合基础-ZK[2022]一般290);农业农村部油料作物生物学与遗传育种重点实验室开放课题项目(KF2020008);财政部和农业农村部国家现代农业产业技术体系建设专项(花生, CARS-13-种质资源评价)

Correlation analysis of sucrose content with protein and oil content and QTL mapping of sucrose content in peanut

GUO Jian-Bin1(), CHENG Liang-Qiang2(), LI Wei-Tao1, LIU Nian1, LUO Huai-Yong1, DING Ying-Bin1, YU Bo-Lun1, CHEN Wei-Gang1, HUANG Li1, ZHOU Xiao-Jing1, LEI Yong1, LIAO Bo-Shou1, JIANG Hui-Fang1()   

  1. 1Oil Crops Research Institute, China Academy of Agricultural Sciences / Key Laboratory of Biology and Genetic Improvement of Oil Crops, the Ministry of Agriculture and Rural Affairs, Wuhan 430062, Hubei, China
    2Guizhou Oil Research Institute, Guizhou Academy of Agricultural Sciences, Guiyang 550006, Guizhou, China
  • Received:2022-11-11 Accepted:2023-02-21 Published:2023-10-12 Published online:2023-03-01
  • Contact: E-mail: peanutlab@oilcrops.cn
  • About author:**Contributed equally to this work
  • Supported by:
    Guizhou Provincial Science and Technology Project (QKHJC-ZK[2022]YB 290);Open Project Program of the Key Laboratory of Biology and Genetic Improvement of Oil Crops, the Ministry of Agriculture and Rural Affairs(KF2020008);China Agriculture Research System of MOF and MARA (Peanut, CARS-13-Germplasm Resource Evaluation)

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摘要：

随着食用型花生市场规模的日益扩大, 食用花生的品质特性备受关注。甜味是影响食用花生风味和口感的重要指标, 而花生甜味主要来源于蔗糖。提高蔗糖含量是培育食用型花生的关键, 定位蔗糖含量相关的QTL, 将为花生高糖分子育种奠定基础。但提高蔗糖可能对蛋白质和含油量有影响。本研究以徐花13和中花6号为亲本构建的重组自交系群体(RIL)为材料, 分析了蔗糖与蛋白质和含油量的关系, 并对蔗糖含量进行了QTL定位。结果表明, 蔗糖含量与蛋白质含量无显著相关性, 而与含油量呈极显著负相关; RIL群体中蔗糖含量变异广泛, 变异范围为14.33~61.42 mg g-1; 筛选出2份高蛋白低脂肪中等含糖量的材料。通过该群体的遗传连锁图, 在3个环境下共检测到10个QTL, 分布在6条染色体上, 贡献率为4.56%~12.25%。其中, qSUCA07能在3个环境下被重复检测到。本研究为蔗糖含量精细定位和培育性状优良的食用型花生品种提供了重要信息。

关键词: 花生, 含油量, 蛋白质, 蔗糖, QTL

Abstract:

With the increasing size of edible peanuts market, the quality characteristics of edible peanuts have attracted much attention. Sweetness is an important indicator affecting the flavor and taste of edible peanuts, and it mainly comes from sucrose. Increasing sucrose content is the key to the edible peanuts cultivation, and QTL mapping for sucrose content will lay a foundation for molecular breeding of high sucrose content. However, the increase of sucrose content may affect protein and oil content. In this study, a recombinant inbred line population derived from the cross of Xuhua 13 and Zhonghua 6 was developed. The relationships between sucrose and protein as well as oil content were investigated, and QTLs for sucrose content were mapped. The results showed that sucrose content was not significantly correlated with protein content but there was extremely significantly negatively correlated with the content. Sucrose content presented board phenotypic variations among the RILs, ranging from 14.33 mg g-1 to 61.42 mg g-1. Two lines with high protein, low oil content, and medium sucrose content were observed. Ten QTLs on six chromosomes were detected with 4.56%-12.25% of phenotypic variation explained (PVE) among which qSUCA07 could be repeatedly detected in three environments. The results provide an important information for QTLs fine mapping of sucrose content and cultivating edible peanut varieties with excellent traits.

Key words: peanut, oil content, protein content, sucrose content, quantitative trait locus

表1

RIL群体蛋白质、含油量和蔗糖含量的相关系数"

环境 性状 蛋白质 含油量 蔗糖
Environment Trait Protein content Oil content Sucrose content
2020WH 蛋白质 Protein content 1 -0.597** -0.169
含油量 Oil content 1 -0.299**
蔗糖 Sucrose content 1
2021WH 蛋白质 Protein content 1 -0.643** -0.132
含油量 Oil content 1 -0.178*
蔗糖 Sucrose content 1
2021QZ 蛋白质Protein content 1 -0.576** -0.021
含油量 Oil content 1 -0.255**
蔗糖 Sucrose content 1
平均 Mean 蛋白质 Protein content 1 -0.647** -0.077
含油量 Oil content 1 -0.443**
蔗糖 Sucrose content 1

图1

亲本蔗糖含量差异 **表示在0.01概率水平差异显著。XH13: 徐花13; ZH6: 中花6号。"

表2

RIL群体蔗糖含量统计分析"

环境
Environment		 最小值
Min.		 最大值
Max.		 均值
Mean		 标准差
SD		 偏度
Skewness		 峰度
Kurtosis		 夏皮洛-威尔克检验
Shapiro-Wilk test W (sig.)
2020WH 16.87 57.93 31.21 9.00 0.74 0.09 0.959 (0.000)
2021WH 14.33 61.42 31.58 8.70 0.81 0.77 0.953 (0.000)
2021QZ 14.49 51.11 32.37 6.49 0.49 0.17 0.978 (0.008)

图2

3个环境下RIL群体蔗糖含量的表型分布 横坐标为蔗糖含量, 纵坐标为频数; 虚线箭头为中花6号, 实线箭头为徐花13。2020WH: 2020武汉; 2021WH: 2021武汉; 2121QZ: 2021泉州。RIL: 重组自交系。"

表3

3个环境下RIL群体蔗糖含量的方差分析"

变异来源 自由度 平方和 均方 F值 P值
Source DF SS MS F-value P-value
环境 Environment 2 134.050 67.025 1.576 0.208
基因型 Genotype 185 20,709.541 111.943 2.633 <0.0001
误差 Error 354 15,050.897 42.517
总计 Total 542 581,263.608

图3

3个环境下RIL群体蔗糖含量性状的QTL峰值图 2020WH: 2020武汉; 2021WH: 2021武汉; 2121QZ: 2021泉州。RIL: 重组自交系。"

表4

3个环境下RIL群体蔗糖含量QTL"

QTL 染色体
Chromosome		 环境
Environment		 位置
Position (cM)		 置信区间
Confidence interval		 LOD值
LOD value		 加性效应
Additive effect		 贡献率
PVE (%)
qSUCA01.1 A01 2020WH 5.11 4.7-7.0 2.62 -1.94 4.60
qSUCA01.2 A01 2020WH 16.01 14.1-17.5 3.00 -2.09 5.23
qSUCA07 A07 2020WH 1.01 0-3.3 6.81 3.21 12.25
2021WH 0.01 0-2.7 4.23 2.49 7.70
2021QZ 0.01 0-3.0 3.51 1.79 7.05
qSUCA10 A10 2020WH 63.70 56.0-64.7 3.60 -2.10 7.14
qSUCB01 B01 2021WH 3.51 0.8-10.9 2.73 -2.06 4.94
qSUCB03.1 B03.1 2020WH 64.81 60.3-66.1 2.70 2.00 4.81
qSUCB10.1 B10 2021QZ 35.71 34.8-37.5 5.47 -2.29 11.45
qSUCB10.2 B10 2021QZ 45.21 43.2-45.6 4.18 -1.98 8.88
qSUCB10.3 B10 2021WH 60.51 59.5-60.7 2.51 -1.95 4.56
qSUCB10.4 B10 2021WH 68.81 67.5-72.7 3.30 -2.22 6.20
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