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Acta Agron Sin ›› 2008, Vol. 34 ›› Issue (12): 2160-2167.doi: 10.3724/SP.J.1006.2008.02160


HMW-GS Accumulation and GMP Size Distribution in Grains of SN12 Grown in Different Soil Conditions

LIANG Tai-Bo12,YIN Yan-Ping1,CAI Rui-Guo1,YAN Su-Hui1,LI Wen-Yang1,GENG Qing-Hui1,WANG Ping1,WU Yun-Hai1,LI Yong1,WANG Zhen-Lin1*   

  1. 1 Agronomy College, Shandong Agricultural University / National Key Laboratory of Crop Biology, Tai’an 271018, Shandong; 2 Zhengzhou Tobacco Research Institute of China National Tobacco Corporation, Zhengzhou 450001, Henan, China
  • Received:2008-04-11 Revised:2008-07-14 Online:2008-12-12 Published:2008-10-10
  • Contact: WANG Zhen-Lin E-mail:zlwang@sdau.edu.cn


Glutenin macropolymer (GMP), the important component of wheat (Triticum aestivum L.) glutenin polymer, consists of high molecular weight gultenin subunits (HMW-GS) and low molecular weight gultenin subunits (LMW-GS). Accumulations of HMW-GS and GMP play key roles in grain quality of wheat. According to recent studies, GMP is a particle network in endosperm of wheat. The content of GMP is affected by HMW-GS accumulation and environment factors. But to date, there are few reports about the relationship between HMW-GS accumulation and GMP particle distribution. To study the HMW-GS accumulation and its relation to GMP particle distribution, a pool experiment was carried out with wheat cultivar Shannong 12 grown under three soil textures. Forty spikes was sampled at 7, 14, 21, 28, and 35 d after anthesis, and partitioned into two groups, superior grain (the 2nd grain from the basal part of a spikelet) and inferior grain (the 4th grain from the basal part of a spikelet). HMW-GS was separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The result showed that all HMW-GS already formed at 14 d after anthesis both in superior grain and inferior grain. Higher HMW-GS content showed that superior grain had strong HMW-GS accumulating ability than inferior grain. HMW-GS accumulation and GMP content of wheat grown in clay loam soil were higher than those in sandy and loam soil, which indicated that clay loam soil was suitable for accumulating of HMW-GS. The diameter of GMP particle was in 0.37–245 μm, it changed in the pattern of two-peak curve in volume and surface area distribution, while single-peak curve in number distribution of GMP particle. The percentages of number and volume of >100 μm GMP particle were higher in superior grain than in inferior grain. The contents of HMW-GS and GMP were negatively correlated with GMP particle volume of <10 μm and <100 μm, but positively correlated with that of >100 μm. It is suggested that larger GMP particle has more HMW-GS content.

Key words: Wheat (Triticum aestivum L.), High molecular weight glutenin subunits (HMW-GS), Glutenin macropolymer (GMP), Size distribution, Soil texture, Superior grain, Inferior grain

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