Welcome to Acta Agronomica Sinica,

Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (12): 2241-2250.doi: 10.3724/SP.J.1006.2011.02241


Effect of Nitrogen Fertilization Timing on HMW-GS Accumulation and GMP Size Distribution in Wheat Grains

CAO Li1,WANG Zhen-Lin1,*,DAI Zhong-Min2,YIN Yan-Ping1,ZHAI Xue-Xu1,NI Ying-Li1,CAI Tie1,LI Yong1,WANG Ping1,CHEN Er-Ying1,GUO Jun-Xiang1,CHEN Xiao-Guang1   

  1. 1 State Key Laboratory of Crop Biology / Agronomy College, Shandong Agricultural University, Tai’an 271018, China; 2 Dezhou College, Dezhou 253023, China
  • Received:2011-04-19 Revised:2011-07-25 Online:2011-12-12 Published:2011-09-29
  • Contact: 王振林, E-mail: zlwang@sdau.edu.cn, zlwangsd@sina.com, Tel: 0538-8241359

Abstract: Wheat processing quality has close relationships with high molecular weight glutenin subunits (HMW-GS) and glutenin macropolyer (GMP) in grains. Nitrogen fertilization scheme could affect the accumulation of HMW-GS and the size distribution of GMP. In this study, we used a strong gluten winter wheat cultivar, Jinan 17, and a weak gluten winter wheat cultivar, Lumai 21, to investigate the effects of nitrogen topdressing stage on the accumulation of HMW-GS and the size distribution of GMP in grains. The nitrogen fertilizer rate during the whole growth period was 225 kg ha-1, of which a half was applied as base fertilizer and the other half was topdressed at pseudo stem erection (SE, GS 30), jointing (JT, GS 32), or booting (BT, GS 41) stage. The spike samples were collected from 7 d after anthesis (DAA) to maturity with a 7-day interval. The HMW-GS in grains formed before 14 DAA, and Jinan 17 had higher contents of HMW-GS and GMP than Lumai 21, which indicated that more glutenin is accumulated in the strong gluten cultivar than in the weak gluten cultivar. At maturity, the contents of HMW-GS and GMP in Jinan 17 showed decreasing trends with the delay of topdressing practice, and the highest values were observed in the SE treatment. In Lumai 21, compared to the SE and BT treatments, the JT treatment significantly accelerated the HMW-GS accumulation at middle-late stage of grain filling, and the rapid accumulation period was prolonged. At middle-late stage of grain filling, the accumulation rates of x-type subunits (1, 4, 5, and 7) were increased significantly in the SE treatment of Jinan 17 and the JT treatment of Lumai 21, whereas the y-type subunits were seldom affected by nitrogen application timging. For both cultivars, delay of nitrogen topdressing enhanced the percentages of volume and surface of GMP particle with diameter less than 12 μm, but reduced those of GMP particle with diameter larger than 100 µm. The percentage of GMP particle number with diameter larger than 12 μm was increased in Jinan 17 with the delay of nitrogen topdressing but decreased in Lumai 21.The percentage of large GMP particle volume was higher in Jinan 17 (containing subunit pair 4+12) than in Lumai 21 (containing subunit pair 5+10). This result suggested that polymerization of subunits and GMP particles are related to not only the subunit type but also the subunit content per unit of flour.

Key words: Wheat, Nitrogen fertilization stage, High molecular weight glutenin subunits (HMW-GS), Glutenin macropolyer (GMP)

[1]Sugiyama T, Rafalski A, Peterson D, Soll D. A wheat HMW glutenin subunit gene reveals a highly repeated structure. Nucl Acids Res, 1985, 13: 8729−8737
[2]Don C, Lichtendonk W J, Plijter J J, Hamer R J. Glutenin macropolymer: a gel formed by glutenin particles. J Cereal Sci, 2003, 37: 1−7
[3]Don C, Lookhart G, Naeem H, MacRitchie F, Hamer R J. Heat stress and genotype affect the gluten in particles of the gluten in macropolymer-gel fraction. J Cereal Sci, 2005, 42: 69−80
[4]Don C, Mann G, Bekes F, Hamer R J. HMW-GS affects the properties of glutenin particles in GMP and thus flour quality. J Cereal Sci, 2006, 44: 127−130
[5]Sun H(孙辉), Yao D-N(姚大年), Li B-Y(李保云), Liu G-T(刘广田), Zhang S-Z(张树臻). Correlation between content of glutenin macropolymer (GMP) in wheat and baking quality. Chin Cereals Oils Assoc (中国粮油学报), 1998, 13(6): 13−16 (in Chinese with English abstract)
[6]Fu B X, Sapirstein H D. Procedure for isolating monomeric proteins and polymeric glutenin of wheat flour. Cereal Chem, 1996, 73: 143−152
[7]MacRitchie F. Conversion of weak flour to a strong one by increasing the proportion of its high molecular weight gluten protein. J Sci Food Agric, 1976, 24: 1325−1329
[8]Gupta R B, Khan K, MacRitchie F. Biochemical basis of flour properties in bread wheat: effects of variation in the quantity and size distribution of polymeric protein. J Cereal Sci, 1993, 18: 23−41
[9]Sapirstein H D, Fu B X. Intercultivar variation in the quantity of monomeric proteins, soluble and insoluble glutenin, and residue protein in wheat flour and relationships to breadmaking quality. Cereal Chem, 1998, 74: 500−507
[10]Zhao H-X(赵惠贤), Hu S-W(胡胜武), Ji W-Q(吉万全), Xue X-Z(薛秀庄), Guo A-G(郭蔼光), Mares D. Study on relationship between the size distribution of glutenin polymeric protein and wheat flour mixing properties. Sci Agric Sin (中国农业科学), 2001, 34(5): 465−468 (in Chinese with English abstract)
[11]Payne P I, Harris P A, Law C N, Holt L M, Blackman J A. The high-molecular-weight subunits of glutenin: Structure, genetics and relationship to bread-making quality. Ann Technol Agric, 1980, 29: 309−320
[12]Zhu J B, Khan K. Characterization of monomeric and glutenin polymeric proteins of hard red spring wheats during grain development by multistacking SDS-PAGE and capillary zone electrophoresis. Cereal Chem, 1999, 76: 261−269
[13]Yue H-W(岳鸿伟), Qin X-D(秦晓东), Dai T-B(戴廷波), Jing Q(荆奇), Cao W-X(曹卫星), Jiang D(姜东). Effects of nitrogen rate on accumulations of HMW-GS and GMP in wheat grain. Acta Agron Sin (作物学报), 2006, 32(10): 1678−1683 (in Chinese with English abstract)
[14]Sutton K H. Qualitative and quantitative variation among high molecular weight subunits of glutenin detected by reverse-phase high -performance liquid chromatography. J Cereal Sci, 1991, 14: 25−34
[15]Kolster P, Krechting C F, Gelder W M J. Quantification of individual high molecular weight glutenin subunits of wheat glutenin using SDS-PAGE and scanning densitometry. J Cereal Sci, 1992, 15: 49−61
[16]Huang D Y, Khan K. Quantitative determination of high molecular weight glutenin subunit of hard red spring wheat by SDS-PAGE: I. Quantitative effects of total amounts on breadmaking quality characteristics. Cereal Chem, 1997, 74: 781−785
[17]Don C, Lichtendonk W J, Plijter J J, Vliet T, Hamer R J. The effect of mixing on glutenin particle properties: aggregation factors that affect gluten function in dough. J Cereal Sci, 2005, 41: 69−83
[18]Shi S-B(石书兵), Ma L(马林), Shi Q-H(石庆华), Liu X(刘霞), Chen L-M(陈乐梅), Liu J-X(刘建喜), Wang Z-L(王振林). Effect of nitrogen application timing on protein constitutes and its dynamic change in wheat grain. Plant Nutr Fert Sci (植物营养与肥料学报), 2005, 11(4): 456−460 (in Chinese with English abstract)
[19]Ma D-Y(马冬云), Guo T-C(郭天财), Yue Y-J(岳艳军), Song X(宋晓), Zhu Y-J(朱云集), Wang C-Y(王晨阳), Wang Y-H(王永华). Effects of nitrogen application at different developmental stages on nitrogen accumulation and translocation in winter wheat. Plant Nutr Fert Sci (植物营养与肥料学报), 2009, 15(2): 262−268 (in Chinese with English abstract)
[20]Weegels P L, van de Pijpekamp A M, Graveland A, Hamer R J, Schofield J D. Depolymerisation and repolymerisation of wheat glutenin during dough processing: I. Relationships between glutenin macropolymer content and quality parameters. J Cereal Sci, 1996, 23: 103−111
[21]Liang R-Q(梁荣奇), Zhang Y-R(张义荣), You M-S(尤明山), Mao S-F(毛善锋), Song J-M(宋建民), Liu G-T(刘广田). Multi-stacking SDS-PAGE for wheat glutenin polymer and its relation to bread-making quality. Acta Agron Sin (作物学报), 2002, 28(5): 609−614 (in Chinese with English abstract)
[22]Zadoks J C, Chang T T, Konzak C F. A decimal code for the growth stages of cereals. Weed Res, 1974, 14: 415−421
[23]Yue H-W(岳鸿伟), Tan W-N(谭维娜), Jiang D(姜东), Dai T-B(戴廷波), Jing Q(荆奇), Cao W-X(曹卫星). Effects of post anthesis drought and waterlogging on contents of high molecular weight glutenin subunits and glutenin macropolymer in wheat grain. Acta Agron Sin (作物学报), 2007, 33(11): 1845−1849 (in Chinese with English abstract)
[24]Bo Y(伯云), Li H-W(李华伟), Mou H-R(牟会荣), Cai J(蔡剑), Zhou Q(周琴), Dai T-B(戴廷波), Cao W-X(曹卫星), Jiang D(姜东). Effects of shading from jointing stage to maturity stage on high molecular weight glutenin subunits accumulation and glutenin macropolymer content in wheat grain. Sci Agric Sin (中国农业科学), 2009, 42(10): 3451−3458 (in Chinese with English abstract)
[25]Zhang D-Y(张定一), Zhang Y-Q(张永清), Yang W-D(杨武德), Yan C-P(闫翠萍), Ji H-T(姬虎太). Effect of nitrogen application on high molecular weight glutenin subunit expression and grain quality of winter wheat. Plant Nutr Fert Sci (植物营养与肥料学报), 2008, 14(2): 235−241 (in Chinese with English abstract)
[26]Payne P I. Genetics of wheat storage proteins and the effect of allelic variation on bread-making quality. Annu Rev Plant Physiol, 1987, 38: 141−153
[27]Zhu J B, Khan K. Quantitative variation of HMW glutenin subunits from hard red spring wheats grown in different environments. Cereal Chem, 2002, 79: 783–786
[28]Wang F-C(王凤成), Zhu J-B(朱金宝), Khan K, Brien L O, Chen W-Y(陈万义). The composition and quantity of wheat glutenin subunits in relation to high molecular weight glutenin polymers and their effects on bread making quality. J Chin Cereals Oils Assoc (中国粮油学报), 2004, 19(3): 13−17 (in Chinese with English abstract)
[1] HU Wen-Jing, LI Dong-Sheng, YI Xin, ZHANG Chun-Mei, ZHANG Yong. Molecular mapping and validation of quantitative trait loci for spike-related traits and plant height in wheat [J]. Acta Agronomica Sinica, 2022, 48(6): 1346-1356.
[2] GUO Xing-Yu, LIU Peng-Zhao, WANG Rui, WANG Xiao-Li, LI Jun. Response of winter wheat yield, nitrogen use efficiency and soil nitrogen balance to rainfall types and nitrogen application rate in dryland [J]. Acta Agronomica Sinica, 2022, 48(5): 1262-1272.
[3] LEI Xin-Hui, WAN Chen-Xi, TAO Jin-Cai, LENG Jia-Jun, WU Yi-Xin, WANG Jia-Le, WANG Peng-Ke, YANG Qing-Hua, FENG Bai-Li, GAO Jin-Feng. Effects of soaking seeds with MT and EBR on germination and seedling growth in buckwheat under salt stress [J]. Acta Agronomica Sinica, 2022, 48(5): 1210-1221.
[4] FU Mei-Yu, XIONG Hong-Chun, ZHOU Chun-Yun, GUO Hui-Jun, XIE Yong-Dun, ZHAO Lin-Shu, GU Jia-Yu, ZHAO Shi-Rong, DING Yu-Ping, XU Yan-Hao, LIU Lu-Xiang. Genetic analysis of wheat dwarf mutant je0098 and molecular mapping of dwarfing gene [J]. Acta Agronomica Sinica, 2022, 48(3): 580-589.
[5] FENG Jian-Chao, XU Bei-Ming, JIANG Xue-Li, HU Hai-Zhou, MA Ying, WANG Chen-Yang, WANG Yong-Hua, MA Dong-Yun. Distribution of phenolic compounds and antioxidant activities in layered grinding wheat flour and the regulation effect of nitrogen fertilizer application [J]. Acta Agronomica Sinica, 2022, 48(3): 704-715.
[6] LIU Yun-Jing, ZHENG Fei-Na, ZHANG Xiu, CHU Jin-Peng, YU Hai-Tao, DAI Xing-Long, HE Ming-Rong. Effects of wide range sowing on grain yield, quality, and nitrogen use of strong gluten wheat [J]. Acta Agronomica Sinica, 2022, 48(3): 716-725.
[7] YAN Yan, ZHANG Yu-Shi, LIU Chu-Rong, REN Dan-Yang, LIU Hong-Run, LIU Xue-Qing, ZHANG Ming-Cai, LI Zhao-Hu. Variety matching and resource use efficiency of the winter wheat-summer maize “double late” cropping system [J]. Acta Agronomica Sinica, 2022, 48(2): 423-436.
[8] WANG Yang-Yang, HE Li, REN De-Chao, DUAN Jian-Zhao, HU Xin, LIU Wan-Dai, GU Tian-Cai, WANG Yong-Hua, FENG Wei. Evaluations of winter wheat late frost damage under different water based on principal component-cluster analysis [J]. Acta Agronomica Sinica, 2022, 48(2): 448-462.
[9] CHEN Xin-Yi, SONG Yu-Hang, ZHANG Meng-Han, LI Xiao-Yan, LI Hua, WANG Yue-Xia, QI Xue-Li. Effects of water deficit on physiology and biochemistry of seedlings of different wheat varieties and the alleviation effect of exogenous application of 5-aminolevulinic acid [J]. Acta Agronomica Sinica, 2022, 48(2): 478-487.
[10] XU Long-Long, YIN Wen, HU Fa-Long, FAN Hong, FAN Zhi-Long, ZHAO Cai, YU Ai-Zhong, CHAI Qiang. Effect of water and nitrogen reduction on main photosynthetic physiological parameters of film-mulched maize no-tillage rotation wheat [J]. Acta Agronomica Sinica, 2022, 48(2): 437-447.
[11] MA Bo-Wen, LI Qing, CAI Jian, ZHOU Qin, HUANG Mei, DAI Ting-Bo, WANG Xiao, JIANG Dong. Physiological mechanisms of pre-anthesis waterlogging priming on waterlogging stress tolerance under post-anthesis in wheat [J]. Acta Agronomica Sinica, 2022, 48(1): 151-164.
[12] MENG Ying, XING Lei-Lei, CAO Xiao-Hong, GUO Guang-Yan, CHAI Jian-Fang, BEI Cai-Li. Cloning of Ta4CL1 and its function in promoting plant growth and lignin deposition in transgenic Arabidopsis plants [J]. Acta Agronomica Sinica, 2022, 48(1): 63-75.
[13] WEI Yi-Hao, YU Mei-Qin, ZHANG Xiao-Jiao, WANG Lu-Lu, ZHANG Zhi-Yong, MA Xin-Ming, LI Hui-Qing, WANG Xiao-Chun. Alternative splicing analysis of wheat glutamine synthase genes [J]. Acta Agronomica Sinica, 2022, 48(1): 40-47.
[14] LI Ling-Hong, ZHANG Zhe, CHEN Yong-Ming, YOU Ming-Shan, NI Zhong-Fu, XING Jie-Wen. Transcriptome profiling of glossy1 mutant with glossy glume in common wheat (Triticum aestivum L.) [J]. Acta Agronomica Sinica, 2022, 48(1): 48-62.
[15] LUO Jiang-Tao, ZHENG Jian-Min, PU Zong-Jun, FAN Chao-Lan, LIU Deng-Cai, HAO Ming. Chromosome transmission in hybrids between tetraploid and hexaploid wheat [J]. Acta Agronomica Sinica, 2021, 47(8): 1427-1436.
Full text



No Suggested Reading articles found!