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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (09): 1592-1606.doi: 10.3724/SP.J.1006.2012.01592


Proteomic Analysis of Leaves of the Chlorophyll-Deficient Wheat Mutant Mt6172 and Its Wild-Type through 2D-Difference Gel Electrophoresis

SONG Su-Jie1,2,**,GU Jia-Yu2,**,GUO Hui-Jun2,ZHAO Lin-Shu2,ZHAO Shi-Rong2,LI Jun-Hui2, ZHAO Bao-Cun1,*,LIU Lu-Xiang2,*   

  1. 1 College of Life Science, Hebei Normal University, Shijiazhuang 050024, China; 2 Institute of Crop Sciences, Chinese Academy of Agricultural Sciences / National Center of Space Mutagenesis for Crop Improvement / National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing 100081, China
  • Received:2012-04-01 Revised:2012-06-06 Online:2012-09-12 Published:2012-07-03
  • Contact: 刘录祥, E-mail: luxiang@263.net.cn, Tel: 010-62122719; 赵宝存, E-mail: baocunzh@126.com

Abstract: Two-dimensional difference gel electrophoresis (2D-DIGE) proteomic approach was used to identify differentially expressed proteins in the leaves of chlorophyll-deficient wheat mutant Mt6172 derived from space environment mutagenesis. A total of 1 654 protein spots were detected, of which 100 spots showed 1.5-fold or higher volume ratio in the leaves of Mt6172 or its wide-type Han 6172. Eighty-five spots subject to mass spectrometry and 62 spots representing 29 distinct proteins were identified and classified into 10 functional groups. Among these differential proteins, 50 were down-regulated and 12 were up-regulated. Most of the down-regulated proteins were located in the subcellular system of chloroplast, including the subunits of PSI, PSII, NAD(P)H dehydrogenase complex and ATP synthase, and the proteins involved in Calvin-Benson cycle, glucose metabolic process and stress responses. Compared to the proteins in chloroplast, the identified non-chloroplast proteins were mainly up-regulated, including the proteins involved in response to oxidative stress, transcriptional activation, and protein folding process. The results suggested that the early death of the albino phenotype of Mt6172 might result from the deficiency of main photosynthesis protein complexes, the decrease of chloroplast antioxidant capacity, and the obstacle of post-transcriptional modification process for chloroplast RNAs.

Key words: Wheat (Triticum aestivum L.), Chlorophyll-deficient mutant, Space mutagenesis, Two-dimensional difference gel electrophoresis (2D-DIGE), Proteomics

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