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Acta Agron Sin ›› 2016, Vol. 42 ›› Issue (05): 696-705.doi: 10.3724/SP.J.1006.2016.00696


Analysis of Heterosis in Sorghum-Sudangrass Hybrids Seedlings Based on Proteomics

HAN Ping-An1,LU Xiao-Ping1,*,MI Fu-Gui2,ZHANG Rui-Xia3,LI Mei-Na3,XUE Chun-Lei1,DONG Jing1,CONG Meng-Lu1   

  1. 1 College of Agronomy, Inner Mongolia Agricultural University, Hohhot 010019, China;2 College of Ecology and Environmental Science, Inner Mongolia
    Agricultural University, Hohhot 010019, China; 3Hohhot Seed Management Station, Hohhot 010020, China
  • Received:2015-09-07 Revised:2016-01-11 Online:2016-05-12 Published:2016-02-18
  • Contact: LU Xiaoping, E-mail: lxpnmnd@126.com E-mail:hanpingan327@163.com
  • Supported by:

    This study was supported by the National Natural Science Foundation of China(31160302,31460375) and the Science and Technology Plan Projects of Hohhot (2012-major plans-8-2).


Sorghum-sudangrass hybrids are typically used for studying heterosis in forage crops. In this study, we carried out proteomic research on sorghum-sudangrass hybrids and their parents at the three-leaf stage by two dimensional electrophoresis-based proteomics and bioinformatic methods. More than 400 protein spots were detected, in which 34 proteins showed significant differences between hybrid and parents in expression, including dominant expression (showing three single-parent silent, seventeen high-parent and five low-parent expression) and overdominant expression (showing one hybrid-specific, six above-high-parent, two below-low-parent expression). Thus, we speculated that dominant and overdominant effects play key roles, and dominant effect is a major factor in the formation of heterosis in sorghum-sudangrass hybrid. Moreover, the 27 out of 34 proteins were related to eight functional categories, i.e., photosynthesis, carbohydrate metabolism, stress response, ATP synthesis, protein synthesis, electron transfer, signal transduction and unknown. The up-regulated photosynthetic proteins were the biggest category, which indicates that photosynthesis in the leaves of sorghum-sudangrass hybrid is enhanced resultig in producing more organic matter, so that showing heterosis. The identified key node proteins in the interaction networks were the potential target proteins for future genetic manipulation of the specific proteins of heterosis. Our findings provide a theoretical basis on heterosis analysis of sorghum-sudangrass hybrids, which is potentially useful for other forage plants.

Key words: Sorghum-sudangrass hybrids, Leaves, Heterosis, Proteomics

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