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Acta Agron Sin ›› 2017, Vol. 43 ›› Issue (04): 608-619.doi: 10.3724/SP.J.1006.2017.00608

• RESEARCH NOTES • Previous Articles     Next Articles

Differential Expression Characteristics of Proteins Involved in Early Development of Maize Grain

YU Tao1,**,LI Geng1,**,ZHANG Cheng-Fen2,LIU Peng1,*,DONG Shu-Ting1,*,ZHANG Ji-Wang1,ZHAO Bin1   

  1. 1 Agronomy College of Shandong Agricultural University / State Key Laboratory of Crop Biology, Tai’an 271018, China; 2 Zibo Zhoucun Vocational Secondary Specialized School, Zibo 255300, China
  • Received:2016-11-01 Revised:2017-01-21 Online:2017-04-12 Published:2017-02-17
  • Contact: liu na,E-mail: liupengsdau@126.com;dong shuting,E-mail: stdong@sdau.edu.cn E-mail:yutaosdnd@163.com
  • Supported by:

    This study was supported by the National Natural Science Foundation of China (31371576, 31401339), the National Key Research and Development Program of China (2016YFD0300106, 2016YFD0300205), the China National Public Welfare Industry (Agriculture) Plan (201203100, 201203096, 201503130), the National Modern Agricultural Technology & Industry System (CARS-02-20), the Shandong Modern Agricultural Technology & Industry System (SDAIT-02-08), the Colleges and Universities of Shandong Province Science and Technology Plan Projects (J14LF10), the Agriculture Technology Innovation Project of Shandong Province and Shandong Provincial Key Laboratory of Corn Breeding and Cultivation Technology.


During early stage of maize grain development, the metabolic activity is strong and the cell division and enlargement processes are also active, leading to increase the grain sink size for subsequent accumulation of storage material. To explore the protein synthesis, accumulation and regulation during early maize grain development, grains of maize cultivar Denghai 661 in the middle of ear were harvested at 3, 5, and 10 days after flowering artificial saturation pollination, respectively, and analyzed by isobaric tags for relative and absolute quantitation (iTRAQ) proteomics. A total of 2639 proteins were identified and quantified in maize grain during early stages of development, showing that these proteins were involved in diverse biological processes and molecular functions, of which the metabolic process and molecular processes were the two most important biological processes, and the catalytic activity and binding were the two most important molecular functions, all of them played important roles in maize grain development. Quantitative analysis showed that 137 proteins significantly differentially expressed during early maize grain development, and these proteins were involved in protein metabolism, stress response, cell growth and division, carbohydrate and energy metabolism, transport, secondary metabolism, starch synthesis, transcription, lipid metabolism, signal transduction and amino acid metabolism. Among them, the proteins expressed more differentially were related to protein metabolism, stress response, cell growth and division, carbohydrate and energy metabolism. Expression pattern clustering analysis showed that these proteins in different functional categories expressed synergically to regulate the early development of maize grain.

Key words: Maize, Grain early development, iTRAQ, Proteomics, Protein function

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