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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (1): 25-32.doi: 10.3724/SP.J.1006.2009.00025

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Genetic Variation of Fatty Acid Components in Arachis Species and development of Interspecific Hybrids with High Oleic and Low Palmitic Acids

JIANG Hui-Fang,REN Xiao-Ping,HUANG Jia-Quan,LEI Yong,LIAO Bo-Shou   

  1. Oil Crops Research Institute,Chinese Academy of Agricultural Sciences,Wuhan 430062,China
  • Received:2008-05-14 Revised:2008-07-17 Online:2009-01-12 Published:2008-11-17

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Abstract:

Wild Arachis species are important resource for genetic improvement of the cultivated peanut (Arachis hypogaea L.). Fatty acid composition is highly crucial for peanut quality in terms of nutritional value and shelf life duration, thus increasing oleate and decreasing the saturated fatty acids such as palmitic and stearic acids has been important breeding objectives worldwide. In the present study, fatty acids of 87 wild Arachis accessions and 113 interspecific hybrid derivatives were tested. Considerable variation in fatty acid components was observed among the Arachis species involved. Among the saturated fatty acids, lowest content of palmitic acid was identified in A. rigonii. Among the unsaturated fatty acids, the highest oleic acid contents were found in A. pusilla and A. duranensis and the highest linoleic acid was in A. batizocoi. Two genotypes (A. duranensis with documented number as 19-6 and A. sp. with documented number as 23-1) with oleic acid content more than 60.0% were identified. Compared to the cultivated peanut, stearic and oleic acid contents were slightly lower and linoleic acid content was slightly higher in the wild species while palmitic acid content was similar to that in A. hypogaea. The interspecific hybrid derivatives had wider ranges of most fatty acids than their parents. The variation of contents of palmitic, stearic, oleic and linoleic acids among the hybrid derivatives was statistically significant. Six derivatives with oleic acid content over 64.0% and palmitic acid content less than 8.5% were identified, among which yz8913-8 had a high oleic acid content as 67.85% (30.0% higher than its parents) and a low palmitic acid as 7.60%. Based on sequence-related amplified polymorphism (SRAP) analysis, new bands were observed in all these 6 derivatives.

Key words: Arachis species, Fatty acids, Genetic diversity, Wide crosses, Genetic enhancement

[1] Wang Y-B(王耀波), Zhang Y-B(张艺兵), Zhang P(张鹏), Men A-J(门爱军). Perspectives and export promoting strategies in Chinese peanut industry after entering WTO. J Peanut Sci (花生学报), 2003, 32(suppl): 24–29(in Chinese)
[2] Liao B-S(廖伯寿). Competitiveness analysis of oil industry in China. J Peanut Sci (花生学报), 2003, 32(suppl): 11–15(in Chinese)
[3] Jiang H-F(姜慧芳), Duan N-X(段乃雄). The corrections of oil and oleic as well as linoleic acids in peanut seeds. Peanut Sci Tech (花生科技), 1993, (2): 4–5(in Chinese)
[4] Liu G-M(刘桂梅), Liang Z-P(梁泽萍). Seed quality of peanut germplasm in China. Oil crops China (中国油料), 1993, 15(1): 18–21(in Chinese)
[5] Jiang H-F(姜慧芳), Ren X-P(任小平), Huang J-Q(黄家权), Liao B-S(廖伯寿), Lei Y(雷永). Establishment of peanut mini core collection in China and finding of new resource with high oleat. Chin J Oil Crops Sci (中国油料作物学报), 2008, 30(3): 287–291(in Chinese with English abstract)
[6] Isleib T G, C T Yong, Knauft D A. Fatty acid genotypes of five Virginia-type cultivars. Crop Sci, 1996, 36: 556–558
[7] Mercer L C, Wynne J C, Young C T. Inheritance of fatty acid content in peanut oil. Peanut Sci, 1990, 17: 17–21
[8] Norden A J, Gorbet D W, Knauft D A, Young C T. Variability in oil quality among peanut genotypes in Florida breeding program. Peanut Sci, 1987, 14: 7–11
[9] Lei Y(雷永), Liao B-S(廖伯寿). Progress of peanut breeding for high oleate. Proceedings of the 5th National Peanut Workshop, Beijing: China Agricultural Science and Technology Press, 2007(in Chinese)
[10] Wan Y-S(万勇善), Tan Z(谭忠), Fan H(范晖), Li X-D(李向东), Zhang G-Y(张高英), Liu F-Z(刘凤珍), Wang S(王溯). Genetic effect of major fatty acid composition in groundnut. Chin J Oil Crops Sci (中国油料作物学报), 2002, 24(1): 26–28(in Chinese with an English abstract)
[11] Tian Y-Q(田永全). Nutritional function of fatty acids. Food Nutr China (中国食物与营养), 2007, (8): 51–52(in Chinese)
[12] Yao Y-Y(姚云游). Comparison of peanut oil and olive oil in nutritional value. China Oil (中国油脂), 2005, (4): 66–68(in Chinese with English abstract)
[13] Jung S, Powell G, Moore K, Annott A. The high oleate trait in cultivated peanut (Arachis hypogaea L.): II. Molecular basis and genetics of the trait. Mol Gen Genet, 2000, 263: 806–811
[14] Jung S, Swift D, Sengoku E, Patel M, Teule F, Powell G. The high oleate trait in the cultivated peanut (Arachis hypogaea L.): I. Isolation and characterization of two genes encoding microsomal oleoyl-PC desaturases. Mol Gen Genet, 2000, 263: 796–805
[15] Lopez Y, Nadaf H L, Smith O D, Connell J P, Reddy A S, Fritz A K. Isolation and characterization of the 12-fatty acid desaturase in peanut (Arachis hypogaea L.) and search for polymorphism for the high oleat trait in Spanish market-type lines. Theor Appl Genet, 2000, 101: 79–805
[16] Lopez Y, Smith O D, Senseman S A, Rooney W L. Genetic factors influencing high oleic acid content in Spanish market-type peanut cultivars. Crop Sci, 2001, 41: 51–56
[17] Lopez Y, Nadaf H L, Smith O D, Simpson C E, Fritz A K. Expressed variants of 12-fatty acid desaturase for the high oleat trait in Spanish market-type peanut lines. Mol Breed, 2002, 9: 183–190
[18] Moore K M, Knauft D A. The inheritance of high oleic acid in peanut. J Hered, 1989, 80: 252–253
[19] Yin D M, Deng S Z, Zhan K H, Cui D Q. High-oleic peanut oils produces by HpRNA-mediated gene silencing of oleate desaturase. Plant Mol Biol Rep, 2007, 25: 154–163
[20] Ding J-P(丁锦平), Han Z-Q(韩柱强), Zhou R-Y(周瑞阳), Gao G-Q(高国庆), Yang Y-P(杨玉萍). Genetic analysis of oleic/linolei (O/L) ratio in peanut. Chin J Oil Crops Sci (中国油料作物学报), 2007, 29(3): 233–237(in Chinese with English abstract)
[21] Yu S-L(禹山林), Isleid T G. The inheritance of high oleic acid content in peanut of virginia type in USA. Chin J Oil Crops Sci (中国油料作物学报), 2000, 22(1): 34–37(in Chinese with English abstract)
[22] Patel M, Jung S, Moore K, Powell G, Ainsworth C, Abbott A. High-oleate peanut mutants result from a MITE insertion into the FAD2 gene. Theor Appl Genet, 2004, 108: 1492–1502
[23] Li D(李铎). Fatty acid components and health. Int Acad Dev (国际学术动态), 2007, (5): 12–13(in Chinese)
[24] Mallikarjuna N. Gene introgression from Arachis glabrata into A. hypogaea, A. duranensis and A. diogoi. Euphytica, 2002, 124: 99–105
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