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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (1): 185-189.doi: 10.3724/SP.J.1006.2009.00185

• RESEARCH NOTES • Previous Articles    

Correlation Analysis between Sucrose Synthase Expression and Sucrose Accumulation in Sweet Sorghum(Sorghum bicolor L.Moench)

YANG Ming1,3,LIU Li-Juan1,LI Li-Yun1,WANG Bo1,CHANG Jin-Hua2,Liu Guo-Zhen1,*   

  1. 1College of Life Sciences,2College of Agronomy,Agricultural University of Hebei,Baoding 071000,China,3 North China Pharmaceutical Group Corporation,Shijiazhuang050015,China
  • Received:2008-05-12 Revised:2008-07-16 Online:2009-01-12 Published:2008-11-18
  • Contact: Liu Guo-Zhen

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2008

Cited

17

Abstract:

Alcohol production by using sweet sorghum (Sorghum bicolor L. Moench) might be a potential way to provide bio-energy in the future, so it is necessary to learn about the mechanism of sucrose accumulation in sweet sorghum for exploitation of bio-energy. The experiment was conducted using two sweet sorghum varieties, to detect expression of sucrose synthase (SS) in leaves and stems by using Western blotting, and analyse the correlation between SS expression and sucrose accumulation. The results indicated that the SS expression in sweet sorghum leaves and stems was lower at early growth stages (jointing and heading stage), increased dramatically at late growth stages (flowering, grain filling and dough stage), and reached the highest level at flowering stage. Compared with two common sorghum varieties, SS expression in leaves and stems in two sweet sorghum varieties increased significantly at late growth stages and correlated with sucrose content in stems. Expression of SS might play an important role in the difference of sucrose content between sweet and common sorghums. The results proposed a strategy for screening sweet sorghum cultivars and enhancing sucrose content of stems in breeding program.

Key words: Sweet sorghum, Common sorghum, Sucrose synthase, Western blotting

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