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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (1): 185-189.doi: 10.3724/SP.J.1006.2009.00185

• RESEARCH NOTES • Previous Articles    

Correlation Analysis between Sucrose Synthase Expression and Sucrose Accumulation in Sweet Sorghum(Sorghum bicolor L.Moench)

YANG Ming1,3,LIU Li-Juan1,LI Li-Yun1,WANG Bo1,CHANG Jin-Hua2,Liu Guo-Zhen1,*   

  1. 1College of Life Sciences,2College of Agronomy,Agricultural University of Hebei,Baoding 071000,China,3 North China Pharmaceutical Group Corporation,Shijiazhuang050015,China
  • Received:2008-05-12 Revised:2008-07-16 Online:2009-01-12 Published:2008-11-18
  • Contact: Liu Guo-Zhen

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Abstract:

Alcohol production by using sweet sorghum (Sorghum bicolor L. Moench) might be a potential way to provide bio-energy in the future, so it is necessary to learn about the mechanism of sucrose accumulation in sweet sorghum for exploitation of bio-energy. The experiment was conducted using two sweet sorghum varieties, to detect expression of sucrose synthase (SS) in leaves and stems by using Western blotting, and analyse the correlation between SS expression and sucrose accumulation. The results indicated that the SS expression in sweet sorghum leaves and stems was lower at early growth stages (jointing and heading stage), increased dramatically at late growth stages (flowering, grain filling and dough stage), and reached the highest level at flowering stage. Compared with two common sorghum varieties, SS expression in leaves and stems in two sweet sorghum varieties increased significantly at late growth stages and correlated with sucrose content in stems. Expression of SS might play an important role in the difference of sucrose content between sweet and common sorghums. The results proposed a strategy for screening sweet sorghum cultivars and enhancing sucrose content of stems in breeding program.

Key words: Sweet sorghum, Common sorghum, Sucrose synthase, Western blotting

[1] Gnansounou E, Dauriat A, Wyman C E. Refining sweet sorghum to ethanol and sugar: economic trade-offs in the context of North China. Bioresour Technol, 2005, 96: 985–1002
[2] Zhang M-F(张明方), Li Z-L(李志凌). Sucrose-metabolizing enzymes in higher plants. Plant Physiol Commun (植物生理学通讯), 2002, 38(3): 289–295 (in Chinese with English abstract)
[3] Tanase K, Yamaki S. Purification and characterization of two sucrose synthase isoforms from Japanese pear fruit. Plant Cell Physiol, 2000, 41: 408–414
[4] Salanoubat M, Belliard G. Molecular cloning and sequencing of sucrose synthase cDNA from potato (Solanum tuberosum L.): preliminary characterization of sucrose synthase mRNA distribution. Gene, 1987, 60: 47–56
[5] McCarty D R, Shaw J R, Hannah L C. The cloning, genetic mapping, and expression of the constitutive sucrose synthase locus of maize. Proc Natl Acad Sci USA, 1986, 83: 9099–9103
[6] Hesse H, Willmitzer L. Expression analysis of a sucrose synthase gene from sugar beet (Beta vulgaris L.). Plant Mol Biol, 1996, 30: 863–872
[7] Sebkova V, Unger C, Hardegger M. Biochemical, physiological, and molecular characterization of sucrose synthase from Daucuscarota. Plant Physiol, 1995, 108: 75–83
[8] Lingle S E, Dyer J M. Cloning and expression of sucrose synthase-1 cDNA from sugarcane. J Plant Physiol, 2001, 158: 129–131
[9] Moriguchi T, Abe K, Yamaki S. Level and role of sucrose synthase, sucrose-phosphate synthase and acid invertase in sucrose accumulation in fruit of Asian pear. J Am Soc Hort Scie, 1992, 117: 274–278
[10] Batta S K, Singh R. Sucroae metabolism in sugarcane growth under varying climatic condition: synthsis and storage of sucrose in relation to the activities of sucrose synthase, sucrose-phosphate synthase and acid invertase. Phytochemistry, 1986, 25: 2431–2437
[11] Moriguchi T, Sanada T, Yamaki S. Seasonal fluctuation of some enzymes relating to sucrose and sorbitol metabolism in peach fruit. J Am Soc Hort Sci, 1990, 115: 278–281
[12] Hu C-M(胡春梅), Wang X-F(王秀峰), Ji J-J(季俊杰), Zhu Y-L(朱月林). Changes in carbohydrate contents during guar plant development. Acta Agron Sin (作物学报), 2007, 33(11): 1869–1873(in Chinese with English abstract)
[13] Qi H-Y(齐红岩), Li T-L(李天来), Zhang J(张洁), Liu H-T(刘海涛). Relation ship between carbohydrate change and related enzymes activities during tomato fruit development. Acta Hort Sin (园艺学报), 2006, 33(2): 294–299(in Chinese with English abstract)
[14] Botha F C, Black K G. Sucrose phosphate synthase and sucrose synthase activity during maturation of internodal tissue in sugarcane. Australia J Plant Physiol, 2000, 27: 81–85
[15] Wolfgang E S, Johann M R, Frederik C B. Protein-level expression and localization of sucrose synthase in the sugarcane culm. Physiol Plant, 2004, 121: 187–195
[16] Kaur K, Gupta A K, Kaur N. Effect of water deficit on carbohydrate status and enzymes of carbohydrate metabolism in seedlings of wheat cultivars. Indian J Biochem Biophys, 2007, 44: 223–230
[17] Geromel C, Ferreira L P, Guerreiro S M C, Cavalari A A, Pot D, Pereira L F P, Leroy T, Vieira L G E, Mazzafera P, Marraccini P. Biochemical and genomic analysis of sucrose metabolism during coffee (Coffea arabica) fruit development. J Exp Bot, 2006, 57: 3243–3258
[18] Privat I, Foucrier S, Prins A, Epalle T, Eychenne M, Kandalaft L, Caillet V, Lin C W, Tanksley S, Foyer C Mccarthy J. Differential regulation of grain sucrose accumulation and metabolism in Coffea arabica (Arabica) and Coffea canephora (Robusta) revealed through gene expression and enzyme activity analysis. New Phytol, 2008, 178(4): 781–797
[19] Bhatia S, Singh R. Interconversion of free sugars in relation to activities of enzymes catalyzing synthesis and cleavage of sucrose in growing stem tissues of sorghum. Indian J Exp Biol, 2001, 39: 1035–1040
[20] Yang M(杨明), Liu L-J(刘丽娟), Li L-Y(李莉云), Wang B(王博), Chang J-H(常金华), Liu G-Z(刘国振). The Correlation analysis of sugar accumulation and SPS expression in sweet sorghum (Sorghum bicolor L. Moench) Stems. Sci Agric Sin (中国农业科学), 2008(in press)(in Chinese with English abstract)
[21] Li D-J(黎大爵), Liao F-S(廖馥荪). Sweet Sorghum and Its Utilization (甜高粱及其利用). Beijing: Science Press, 1992. pp 144–145(in Chinese)
[22] Zhang Y-J(张意静). Food Analysis Technology (食品分析技术). Beijing: China Light Industry Press, 2001. pp 138–151(in Chinese)
[23] Zhang C-G(张成岗), Yuan S-J(袁守军), Deng M-Y(邓美玉), Li L(李林), Tang Z-M(汤仲明), He F-C(贺福初). A simple method for semi-quantitative image analysis of dot and band signals. Chin J Stereol Image Anal (中国体视学与图像分析), 2001, 6(3): 167–170(in Chinese with English abstract)
[24] Liu P(刘鹏), Hu C-H(胡昌浩), Dong S-T(董树亭), Wang K-J(王空军), Zhang J-W(张吉旺), Zhang B-R(张保仁). Comparison of enzymes activity associated with sucrose metabolism in the developing grains between sweet corn and normal corn. Sic Agric Sin (中国农业科学), 2005, 38(1): 52–58(in Chinese with English abstract)
[25] Klotza K L, Haagenson D M. Wounding, anoxia and cold induce sugarbeet sucrose synthase transcriptional changes that are unrelated to protein expression and activity. J Plant Physiol, 2008, 165: 423–434
[26] Martin T, Frommer W B, Salanoubat M, Willmitzer L. Expression of an Arabidopsis sucrose synthase gene indicates a role in metabolization of sucrose both during phloem loading and in sink organs. Plant J, 1993, 4: 367–377
[27] Godt D, Roitsch T. The developmental and organ specific expression of sucrose cleaving enzymes in sugar beet suggests a transition between apoplasmic and symplasmic phloem unloading in the tap roots. Plant Physiol Biochem, 2006, 44: 656–665
[28] Farrar J F. Sink strength: what is it and how do we measure it? Plant Cell Environ, 1993, 16: 1013–1046
[29] Li W-Y(李文阳), Yin Y-P(尹燕枰), Yan S-H(闫素辉), Dai Z-M(戴忠民), Li Y(李勇), Liang T-B(梁太波), Geng Q-H(耿庆辉), Wang Z-L(王振林). Effect of shading after anthesis on starch accumulation and activities of the related enzymes in wheat grain. Acta Agron Sin (作物学报), 2008, 34(4): 632–640(in Chinese with English abstract)
[30] Song J(宋瑾), Fan P-G(范培格), Wu B-H(吴本宏), Li S-H(李绍华). Changes in soluble sugars and activities of related metabolic enzymes in grape berries during ripening and delayed harvest. Acta Hort Sin (园艺学报), 2007, 34(4): 823–828(in Chinese with English abstract)
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