Welcome to Acta Agronomica Sinica,

Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (2): 228-238.doi: 10.3724/SP.J.1006.2009.00228


Genetic Structure and Diversity of Soybean Germplasm in Heilongjiang in China

QIN Jun2,3;LI Ying-Hui1;LIU Zhang-Xiong1;LUAN Wei-Jiang1;YAN Zhe1;GUAN Rong-Xia1;ZHANG Meng-Chen3;CHANG Ru-Zhen1;LI Guang-Min2,3,*,   

  1. 1National Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Germplasm & Biotechnology, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081,China;2 Agricultural University of Hebei, Baoding 071000,China; 3Hebei Academy of Agricultural and Foretry Science,Shijiazhuang 050031,China
  • Received:2008-02-29 Revised:2008-09-10 Online:2009-02-12 Published:2008-12-10
  • Contact: QIU Li-Juan


Heilongjiang province is the main production area for soybean (Glycine max) in China, having the high genetic diversity of cultivated soybean. It is useful for breeding and production in the region to develop and create new germplasm. The aim of this study was to reveal genetic structure and genetic diversity of spring seeding soybean germplasm from Heilongjiang, and provide a reliable strategy in soybean breeding program. A total of 140 accessions of soybean cultivars including 78 landraces and 62 developed cultivars were investigated using 60 microsatellite(simple sequence repeat, SSR) markers and 22 phenotypic traits. All accessions were grouped into two clusters based on SSR with UPGMA and Model-base. The results showed that genetic diversity of clusterII was higher than that of clusterI, Nei’s genetic distance was 0.2427 between two clusters. PCO (principlal co-ordinate) analysis revealed that two clusters distributed in different regions, which was closely related to the geographic origin and breeding years. All accessions were also divided into two groups (developed cultivars and landraces) based on variety types, genetic diversity of the landraces was higher than that of developed cultivars. Nei’s genetic distance was 0.1131 between two groups. The PCO figure based on the phenotypic genetic similarity index matrix clustered 140 cultivars into developed cultivars group and landrace group. The first principal component reflected main seed coat color; the second one mainly depended on growth period and maturity data. There were obvious differences on the three principal components, which were composed of six phenotypic characters, between developed group and landraces group, which were not independent genetic clusters. The results indicated that there was abundant genetic diversity in Heilongjiang spring sown soybeans. Thus the optimal strategy combined SSR data and agronomic traits is necessary for genetic diversity analysis of soybean germplasm.

Key words: Heilongjiang province, Soybean cultivars, SSR, Genetic structure, Genetic diversity

[1] Lin H(林红), Lai Y-C(来永才), Qi N(齐宁), Li H(李辉), Zhang X-B(张晓波), Yang X-F(杨雪峰). Screening of germplasm with high content of isoflavones in wild and cultivated soybean in Heilongjiang. J Plant Genet Resour (植物遗传资源学报), 2005, 6(1): 53-55 (in Chinese with English abstract)
[2] Wang Z-Y(王子迎), Wang Y-C(王源超), Zhang Z-G(张正光), Zheng X-B(郑小波). Genetic relationships among Chinese and American isolates of Phytophthora sojae by ISSR markers. Biodivers Sci(生物多样性), 2007, 15(3): 215-223 (in Chinese with English abstract)
[3] Xue E-Y(薛恩玉), Li W-H(李文华), Jiang Y (姜妍). Evolution tendency of agronomic characters of soybean cultivars released in Heilongjiang province. Soybean Sci (大豆科学), 2006, 25(4): 445-449 (in Chinese with English abstract)
[4] Qiu L-J(邱丽娟), Cao Y-S(曹永生), Chang R-Z(常汝镇), Zhou X-A(周新安), Wang G-X(王国勋), Sun J-Y(孙建英), Xie H(谢华), Zhang B(张博), Li X-H(李向华), Xu Z-Y(许占有), Liu L-H(刘立宏). Establishment of Chinese soybean (Glycine max) core collection I: Sampling strategy. Sci Agric Sin (中国农业科学), 2003, 36(12): 1442-1449 (in Chinese with English abstract)
[5] Wang L X, Guan Y, Guan R X, Li Y H, Ma Y S, Dong Z M, Liu X, Zhang H Y, Zhang Y Q, Liu Z X, Chang R Z, Xu H M, Li L H, Lin F Y, Luan W J, Yan Z, Ning X C, Zhu L, Cui Y H, Piao R H, Liu Y, Chen P Y, Qiu L J. Establishment of Chinese soybean (Glycine max) core collections with agronomic traits and SSR markers. Euphytica, 2006, 151: 215-223
[6] Xue H(谢华), Chang R-Z(常汝镇), Cao Y-S(曹永生), Zhang M-H(张明辉), Feng Z-F(冯忠孚), Qiu L-J(邱丽娟). Selection of core SSR loci by using Chinese autumn soybean. Sci Agric Sin (中国农业科学), 2003, 36(4): 360-366(in Chinese with English abstract)
[7] Xie H(谢华), Guan R-X(关荣霞), Chang R-Z(常汝镇), Qiu L-J(邱丽娟). Genetic diversity of Chinese summer soybean
[Glycine max (L.) Merr.] germplasm revealed by SSR markers. Chin Sci Bull (科学通报), 2005, 52(5): 343-351( in Chinese )
[8] Cui Y-H(崔艳华), Qiu L-J(邱丽娟), Chang R-Z(常汝镇), Lü W-H(吕文河). A study of genetic diversity of Huanghuai summer sowing soybean in China. Sci Agric Sin (中国农业科学), 2004, 37(1): 15-22(in Chinese with English abstract)
[9] Li Y-H(李英慧), Liu Y(刘燕), Guan R-X(关荣霞), Wei S-H(魏淑红), Yang G-Y(杨光宇), Zhou X-A(周新安), Zhang M-C(张孟臣), Yang C-Y(杨春燕), Zhu B-G(朱保葛), Li W-D(李卫东), Liu X-Y(刘学义), Xu R(徐冉), Sun J-M(孙君明), Zhu S-L(朱申龙), Zhao T-J(赵团结), Liu Z-X(刘章雄), Chang R-Z(常汝镇), Qiu L-J(邱丽娟). Genetic structure and diversity of both enhanced germplasms developed during 10th five-year plan and modern cultivars released during 1963-1995 in China. Acta Agron Sin (作物学报), 2007, 33(10): 1630-1636(in Chinese with English ab-stract)
[10] Wang L-X(王丽侠). Genetic diversity analysis and establishment of core collection of Glycine max in China (中国栽培大豆遗传多样性分析与核心种质构建). PhD Dissertation of Chinese Academy of Agricultural Sciences, 2005. pp 61-73(in Chinese with English abstract)
[11] Institute of Crop Germplasm Resource, Chinese Academy of Agricultural Sciences (中国农科院作物品种资源所), Catalog of Chinese Soybean Germplasm Resources(Continued 1, 2)
[中国大豆品种资源目录(续编一、二)]. Beijing: Agriculture Press, 1990, 1996
[12] Rohlf F J. NTSYS: Numerical taxonomy and multivariate analy-sis system, Version 2.1, New York: State University of New York, 2000
[13] Wang B (王彪), Chang R-Z(常汝镇), Tiao L(陶莉), Guan R-X(关荣霞), Yan L(阎丽), Zhang M-H(张明恢), Feng Z-F(冯忠孚), Qiu L-J(邱丽娟). Identification of SSR primer numbers for analyzing genetic diversity of Chinese cultivated soybean. Mol Plant Breed (分子植物育种), 2003, 1(1): 82-88(in Chinese with English abstract)
[14] Guan R-X(关荣霞), Chang R-Z(常汝镇), Qiu L-J(邱丽娟). Rapid isolation of soybean DNA for SSR analysis. Soybean Sci (大豆科学), 2003, 22(1): 73-74(in Chinese with English abstract)
[15] Evanno G, Regnaut S, Goudet J. Detecting the number of clusters of individuals using the software structure: A simulation study. Mol Ecol, 2005, 14: 2611-2620
[16] Yeh F C, Yang R C. POPGENE Version 1.31, available at http://www. ualberta.ca/~fyeh/popgene.pdf, 1999
[17] Narvel J M, Fehr W R, Chu W C, Grant D, Shoemarker R C. Simple sequence repeat diversity among soybean plant introduc-tions and elite genotypes. Crop Sci, 2000, 40: 1452 -1458
[18] Luan W-J(栾维江), Liu Z-X(刘章雄), Guan R-X(关荣霞), Chang R-Z(常汝镇), He B-R(何蓓如), Qiu L-J(邱丽娟). Repre-sentativeness of northeast China spring soybeans and their ge-netic diversity at SSR loci. Chin J Appl Ecol (应用生态学报), 2005, 16(8): 1469-1476 (in Chinese with English abstract)
[19] Sun Z-Q(孙志强), Tian P-Z(田佩占), Wang J-A(王继安). Analy-sis of the parentage compositions among soybean cultivars de-veloped in the northeast of China. Soybean Sci (大豆科学), 1990, 9(2): 112-120(in Chinese with English abstract)
[20] Gai J-Y(盖钧镒), Wang Y-S(汪越胜). A study on the varietal ecoregions of soybeans in China. Sci Agric Sin (中国农业科学), 2001, 34(2): 139-145(in Chinese with English abstract)
[21] Chen W-Y(陈维元), Lü D-C(吕德昌), Jiang C-X(姜成喜), Fu Y-S(付亚书), Jing Y-L(景玉良), Fu C-X(付春旭). Pedigree analysis of soybean cultivars named by Suinong. Heilongjiang Agric Sci (黑龙江农业科学), 2004, (4): 9-12 (in Chinese with English abstract)
[22] Hu X-P(胡喜平). Analysis of family tree of Hefeng series of soybean varieties. Soybean Sci (大豆科学), 2002, 21(2): 131-137(in Chinese with English abstract)
[23] Cui Y-H(崔艳华), Qiu L-J(邱丽娟), Chang R-Z(常汝镇), Lü W-H(吕文河). Advances in the core collection of plant germ-plasm resources. J Plant Genet Resour (植物遗传资源学报), 2003, 4(3): 279-284 (in Chinese with an English abstract)
[24] Dong Y-S(董英山), Lü J-L(吕景良), Jiang X-Z(江相智). Analy-sis center of origin of soybean (Glycine max) in China. Crops (作物杂志), 1998, (1): 18-19(in Chinese)
[25] Diwan N, Cregan P B. Automated sizing of fluorescent-labeled simple sequence repeat (SSR) markers to assay genetic variation in soybean. Theor Appl Genet, 1997, 95: 723-733
[26] Ma Y S, Wang W H, Wang L X, Ma F M, Wang P W, Chang R Z, Qiu L J. Genetic diversity of soybean and establishment of core collection focused on resistance to soybean cyst nematode. J Integr Plant Biol, 2006, 48: 722-731
[27] Zhang D-L(张冬玲), Zhang H-L(张洪亮) , Wei X-H(魏兴华), Qi Y-W(齐永文), Wang M-X(王美兴), Sun J-L(孙俊立), Ding L(丁立), Tang S-X(汤圣祥), Qiu Z-E(裘宗恩), Cao Y-S(曹永生), Wang X-K(王象坤), Li Z-C(李自超). Genetic structure and di-versity of Oryza sativa L. in Guizhou, China. Chin Sci Bull (科学通报), 2006, 51(23): 343-351(in Chinese)
[28] He T-M(何天明), Chen X-S(陈学森), Gao J-S(高疆生), Zhang D-H(张大海), Xu L(徐麟), Wu Y(吴燕). Using SSR markers to study population genetic structure of cultivated apricots native to Xinjiang. Acta Hort Sin (园艺学报), 2006, 33(4): 809-812(in Chinese with English abstract)
[1] XIAO Ying-Ni, YU Yong-Tao, XIE Li-Hua, QI Xi-Tao, LI Chun-Yan, WEN Tian-Xiang, LI Gao-Ke, HU Jian-Guang. Genetic diversity analysis of Chinese fresh corn hybrids using SNP Chips [J]. Acta Agronomica Sinica, 2022, 48(6): 1301-1311.
[2] CHEN Xiao-Hong, LIN Yuan-Xiang, WANG Qian, DING Min, WANG Hai-Gang, CHEN Ling, GAO Zhi-Jun, WANG Rui-Yun, QIAO Zhi-Jun. Development of DNA molecular ID card in hog millet germplasm based on high motif SSR [J]. Acta Agronomica Sinica, 2022, 48(4): 908-919.
[3] ZHANG Xia, YU Zhuo, JIN Xing-Hong, YU Xiao-Xia, LI Jing-Wei, LI Jia-Qi. Development and characterization analysis of potato SSR primers and the amplification research in colored potato materials [J]. Acta Agronomica Sinica, 2022, 48(4): 920-929.
[4] WANG Yan-Yan, WANG Jun, LIU Guo-Xiang, ZHONG Qiu, ZHANG Hua-Shu, LUO Zheng-Zhen, CHEN Zhi-Hua, DAI Pei-Gang, TONG Ying, LI Yuan, JIANG Xun, ZHANG Xing-Wei, YANG Ai-Guo. Construction of SSR fingerprint database and genetic diversity analysis of cigar germplasm resources [J]. Acta Agronomica Sinica, 2021, 47(7): 1259-1274.
[5] HAN Bei, WANG Xu-Wen, LI Bao-Qi, YU Yu, TIAN Qin, YANG Xi-Yan. Association analysis of drought tolerance traits of upland cotton accessions (Gossypium hirsutum L.) [J]. Acta Agronomica Sinica, 2021, 47(3): 438-450.
[6] LIU Shao-Rong, YANG Yang, TIAN Hong-Li, YI Hong-Mei, WANG Lu, KANG Ding-Ming, FANG Ya-Ming, REN Jie, JIANG Bin, GE Jian-Rong, CHENG Guang-Lei, WANG Feng-Ge. Genetic diversity analysis of silage corn varieties based on agronomic and quality traits and SSR markers [J]. Acta Agronomica Sinica, 2021, 47(12): 2362-2370.
[7] SUN Qian, ZOU Mei-Ling, ZHANG Chen-Ji, JIANG Si-Rong, Eder Jorge de Oliveira, ZHANG Sheng-Kui, XIA Zhi-Qiang, WANG Wen-Quan, LI You-Zhi. Genetic diversity and population structure analysis by SNP and InDel markers of cassava in Brazil [J]. Acta Agronomica Sinica, 2021, 47(1): 42-49.
[8] GUO Yan-Chun, ZHANG Li-Lan, CHEN Si-Yuan, QI Jian-Min, FANG Ping-Ping, TAO Ai-Fen, ZHANG Lie-Mei, ZHANG Li-Wu. Establishment of DNA molecular fingerprint of applied core germplasm in jute (Corchorus spp.) [J]. Acta Agronomica Sinica, 2021, 47(1): 80-93.
[9] Meng-Liang ZHAO,Li-Hui WANG,Yan-Jing REN,Xue-Mei SUN,Zhi-Qiang HOU,Shi-Peng YANG,Li LI,Qi-Wen ZHONG. Genetic diversity of phenotypic traits in 257 Jerusalem artichoke accessions [J]. Acta Agronomica Sinica, 2020, 46(5): 712-724.
[10] WANG Heng-Bo,QI Shu-Ting,CHEN Shu-Qi,GUO Jin-Long,QUE You-Xiong. Development and application of SSR loci in monoploid reference genome of sugarcane cultivar [J]. Acta Agronomica Sinica, 2020, 46(4): 631-642.
[11] Hong-Yan ZHANG,Tao YANG,Rong LIU,Fang JIN,Li-Ke ZHANG,Hai-Tian YU,Jin-Guo HU,Feng YANG,Dong WANG,Yu-Hua HE,Xu-Xiao ZONG. Assessment of genetic diversity by using EST-SSR markers in Lupinus [J]. Acta Agronomica Sinica, 2020, 46(3): 330-340.
[12] Li-Lan ZHANG, Lie-Mei ZHANG, Huan-Ying NIU, Yi XU, Yu LI, Jian-Min QI, Ai-Fen TAO, Ping-Ping FANG, Li-Wu ZHANG. Correlation between SSR markers and fiber yield related traits in jute (Corchorus spp.) [J]. Acta Agronomica Sinica, 2020, 46(12): 1905-1913.
[13] MA Yan-Ming, FENG Zhi-Yu, WANG Wei, ZHANG Sheng-Jun, GUO Ying, NI Zhong-Fu, LIU Jie. Genetic diversity analysis of winter wheat landraces and modern bred varieties in Xinjiang based on agronomic traits [J]. Acta Agronomica Sinica, 2020, 46(12): 1997-2007.
[14] LIU Rong, WANG Fang, FANG Li, YANG Tao, ZHANG Hong-Yan, HUANG Yu-Ning, WANG Dong, JI Yi-Shan, XU Dong-Xu, LI Guan, GUO Rui-Jun, ZONG Xu-Xiao. An integrated high-density SSR genetic linkage map from two F2 population in Chinese pea [J]. Acta Agronomica Sinica, 2020, 46(10): 1496-1506.
[15] MA Yan-Ming, LOU Hong-Yao, CHEN Zhao-Yan, XIAO Jing, XU Lin, NI Zhong-Fu, LIU Jie. Genetic diversity assessment of winter wheat landraces and cultivars in Xinjiang via SNP array analysis [J]. Acta Agronomica Sinica, 2020, 46(10): 1539-1556.
Full text



No Suggested Reading articles found!