Peanut (Arachis hypogaea L.),SSR,Genetic linkage map,"/> Construction of Genetic Linkage Map in Peanut(<em>Arachis hypogaea</em> L.) Cultivars
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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (3): 395-402.doi: 10.3724/SP.J.1006.2009.00395

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Construction of Genetic Linkage Map in Peanut(Arachis hypogaea L.) Cultivars

HONG Yan-Bin;LIANG Xuan-Qiang*;CHEN Xiao-Ping;LIU Hai-Yan;ZHOU Gui-Yuan;LI Shao-Xiong;WEN Shi-Jie   

  1. Crops Research Institute, GuangdongAcademy of Agricultural Sciences,Guangzhou 510640,China
  • Received:2008-05-16 Revised:2008-09-11 Online:2009-03-12 Published:2009-01-15
  • Contact: LIANG Xuan-Qiang

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Abstract:

Molecular genetic map is a fundamental organizational tool for genomic research. However, a comprehensive genetic linkage map for peanut cultivars has not yet been developed due to extremely low levels of DNA polymorphisms in cultivated peanut. In this study, 184 recombinant inbred lines (RIL), derived from a cross between two Spanish type peanut cultivars (Yueyou 13 × Fu95-5), were used as mapping population. A total of 652 pairs of genomic-SSR primers and 392 pairs of EST-SSR primers were used to detect the polymorphisms between the parental cultivars. Of them, 121 SSR primer pairs amplified 123 segregating loci and were selected to analyze the RIL population. A genetic linkage map consisting of 108 SSR loci (102 genomic-SSR and 6 EST-SSR) in 20 linkage groups and covers 568 cM with an average distance of 6.45 cM of intermaker was constructed. By comparing the SSR genetic map of Arachis (A. duranensis × A. stenosperma) with AA genome, 11 linkage groups in the linkage map constructed in this study were confirmed to have homology with 6 linkage groups of wild peanut species.

Key words: Peanut (Arachis hypogaea L.)')">Peanut (Arachis hypogaea L.), SSR, Genetic linkage map

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