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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (3): 388-394.doi: 10.3724/SP.J.1006.2009.00388

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Analysis of QTLs for Resistance to Small Brown Planthopper(Laodelphax striatellus Fallen)in Rice(Oryza sativa L.) Using an F2 Population from a Cross between Mudgo and Wuyujing 3

DUAN Can-Xing1;CHENG Zhi-Jun1;LEI Cai-Lin1;ZHAI Hu-Qu2;WAN Jian-Min1*   

  1. Institute of Crop Sciences,Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement,Beijing 100081,China;Chinese Academy of Agricultural Sciences,Beijing 100081,China
  • Received:2008-10-13 Revised:2009-01-07 Online:2009-03-12 Published:2009-01-15
  • Contact: WAN Jian-Min

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Abstract:

The small brown planthopper (SBPH), Laodelphax striatellus Fallén (Homoptera: Delphacide), is an economically important pest in rice (Oryza sativa L.) and distributes widely in China. It not only causes direct damage by sucking plant sap but also transmits several viral diseases such as Rice stripe virus(RSV) and Rice black-streaked dwarf virus(RBSDV). which often cause major yield losses.. Host resistance has been recoqnized as one of the most economic and effective measures in controlling SBPH. Mudgo is one of indica rice cultivars with high resistance to SBPH, expressing strong antixenosis and antibiosis against SBPH. A genetic linkage map was constructed with an F2 population, derived from a cross between Mudgo and a japonics cultivar Wuyujing 3 for mapping QTLs associated with resistance to SBPH. The linkage map comprised 104 SSR and 3 Indel markers and covered 1 409.9 cM of rice genome with an average marker interval of 13.2 cM. One hundred and seventy-seven plants of F2:3 families were identified for resistance to SBPH by way of the modified seedbox screening test. QTL analysis of SBPH resistance was conducted using composite interval mapping implemented in Windows QTL Cartographer 2.5 software. A total of three QTLs, Qsbph2b, Qsbph3d, and Qsbph12a,conferring resistance to SBPH were detected on chromosomes 2, 3, and 12, locating in the regions of RM5791–RM29, RM3199–RM5442, and I12-17–RM3331, with LOD scores of 3.25, 3.11, and 6.82, respectively. The resistant alleles of Qsbph2b, Qsbph3d, and Qsbph12a, were all from Mudgo and could explain 17.3%, 15.6%, and 35.8% of total phenotypic variance, respectively. Qsbph12a linked tightly to the markers RM3331 and I12-17 has a potential value in breeding for SBPH resistance by rapid marker-assisted selection.

Key words: Rice, Resistance, Small brown planthopper, Quantitative trait locus(QTL)

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