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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (6): 1044-1050.doi: 10.3724/SP.J.1006.2009.01044


Genetical Studies on Progenies of Somatic Hybrids between Brassica napus and Descurainia sophia

XIN Ru-Ying,GUAN Rong-Zhan*,ZHANG Li-Jun,JIANG Shu-Hui,ZHANG Hong-Sheng,ZHENG Xiu   

  1. State Key Laboratory of Crop Genetics and Germpalsm Enhancement,Nanjing Agricultural University,Nanjing 210095,China
  • Received:2008-12-07 Revised:2009-02-17 Online:2009-06-12 Published:2009-04-16
  • Contact: GUAN Rong-Zhan,E-mail:guanrzh@njau.edu.cn


Somatic hybridization is an available way to create new germplasm and enrich breeding materials. Somatic hybridization between B. napus and D. sophia has been finished by the authors in 2005. Present research is to continue progeny studies of the somatic hybrids with the objective of carrying out desirable genes transfer from D. sophia to B. napus. Fertile progenies of the somatic hybrids between Brassica napus (2n=38) and Descurainia sophia (2n=38) selfed in three consecutive generations, resulting in F2, F3, and F4 populations. The number of somatic chromosome, chromosome behavior and genetic components from D. sophia in these progenies were observed and analyzed by cytological and SSR analyse. The average number of chromosomes in F2, F3, and F4 populations were 38.47±3.17, 37.65±3.23, and 36.66±2.95, respectively, and the maximum number was 56, 50, and 45, respectively, showing that chromosome number tended to reduction with the generation progression. Abnormal chromosome behaviors such as chromosome bridges, lagging chromosomes, meiotic cycle asynchrony, chromosome unequal distribution at meiosis stage were often observed in the three populations. SSR marker detections with 22 pairs of primers showed that the average frequency of plants with bands characteristic of D. sophia in the F2, F3, and F4 populations was 9.62%, 2.99%, and 0.31%, respectively. Therefore, achieving germplasm of D. sophia filter into B. napus should attach importance to select progenies of the somatic hybrid in F2 population.

Key words: Brassica napus, Descurainia Sophia, Somatic hybrid, Chromosome behavior, SSR

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