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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (9): 1590-1596.doi: 10.3724/SP.J.1006.2009.01590


Genetic Composition of Elite Soybean Dultivar Hefeng 25

GUAN Rong-Xia1,QIN Jun2,HU Jing-Shen3,CHEN Wei-Xiu3,CHANG Ru-Zhen1,LIU Zhang-Xiong,QIU Li-Juan1   

  1. 1 National Key Facility for Crop Gene Resources and Genetic Improvement / Key Laboratory of Germplasm Utilization, Ministry of Agriculture / Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 2 Hebei Academy of Agricultural and Forestry Science, Shijiazhuang 050031, China; 3 Northeast Agricultural University, Harbin 150030, China
  • Received:2009-01-08 Revised:2009-04-26 Online:2009-09-12 Published:2009-07-03
  • Contact: QIU Li-Juan, E-mail: qiu_lijuan@263.net; Tel: 010-82105843


Elite soybean cultivar Hefeng 25, derived from a cross of Hefeng 23×Ke 4430-20, has an accumulated planting area of 13 million hectares and the longest planting history of soybean cultivars. The objective of this study is to evaluate the genetic composition of Hefeng 25 and the relationship of Hefeng 25 with the parents. Four hundred and sixty-three SSR markers were used to screen Hefeng 25 and its parents Hefeng 23 and Ke 4430-20. Of the 463 SSR markers 177 were monomorphic between Hefeng 23 and Ke 4430-20 and 57 loci were detected in Hefeng 25 with new alleles mutated not from the parents. The genetic contribution of Hefeng 23 and Ke 4430-20 to Hefeng 25 was 39.4% and 48.3%, respectively based on molecular information. Analysis of each linkage group revealed that large portions of loci on linkage groups G, E, and L were inherited entirely from one parent. Especially on linkage group G, all loci were inherited from Ke4430-20. The loci on linkage group L inherited from Hefeng 23 were 2.3 fold of that from Ke4430-20. Detailed analysis showed that QTLs for yield and disease resistance may relate to these loci. These analyses suggested that breeders may select recombination events with agronomic favorable alleles of two parents. In order to evaluate the effect of SSR mutation on soybean seed purity, we screened Hefeng 25 seed samples from 12 different seed companies at 207 SSR loci including 57 mutated loci. Hybrid alleles were observed only in 4 seed samples at 13 SSR loci. The result indicated that most of the mutated loci were purified during the breeding process. The elite genotype and high seed purity of Hefeng25 may be the most important factors for its long-term utilization in soybean production.

Key words: Soybean(Glycine max), Hefeng 25, Pedigree, SSR, Genetic composition

[1] Guo T(郭泰), Liu Z-T(刘忠堂), Qi N(齐宁), Zhang R-C(张荣昌), Hu X-P(胡喜平). Breeding and utilization of high-yield soybean Hefeng 25. Soybean Sci (大豆科学), 1997, 16(1): 85-87 (in Chinese)

[2] Guo T(郭泰), Liu Z-T(刘忠堂), Qi N(齐宁), Zhang R-C(张荣昌), Hu X-P(胡喜平), Wang Z-X(王志新). Utilization of Hefeng25 in soybean breeding. Crop Germplasm Resour (作物品种资源), 1998, (2): 19-20 (in Chinese)

[3] Liu C-H(刘长海), Yu X-C(于晓春), Qiu C-J(邱长久), Zhang A-H(张安宏), Cheng H-Y(成华玉), Hu S-Y(胡淑艳). Utilization of Hefeng 25 as parental material. Soybean Bull (大豆通报), 2001, (3): 22 (in Chinese)

[4] Qiu L-J(邱丽娟), Wang S-M(王曙明). Chinese soybean breeding lines (1993-2004), Beijing: China Agricultural Press, 2008 (in Chinese)

[5] Qiu F-L(邱福林), Shao G-J(邵国军), Li Y-D(李跃东), Han Y(韩勇). Parent selection and pedigree analysis of Liaojing 326. Reclaim Rice Cult (垦殖与稻作), 2001, (3): 3-5 (in Chinese)

[6] Qin J(秦君), Jiang C-X(姜成喜), Liu Z-X(刘章雄), Fu Y-S(付亚书), Guan R-X(关荣霞), Chen W-Y(陈维元), Li Y-H(李英惠), Zhang M-C(张孟臣), Jing Y-L(景玉良), Chang R-Z(常汝镇), Qiu L-J(邱丽娟). Genetic diversity and recombination of soybean cultivar Suinong 14 and its pedigree. Hereditas (遗传), 2006, 28(11): 1421-1427 (in Chinese with English abstract)

[7] Qin J(秦君), Chen W-Y(陈维元), Guan R-Y(关荣霞), Jiang C-X(姜成喜), Li Y-H(李英慧), Fu Y-S(付亚书), Liu Z-X(刘章雄), Zhang M-C(张孟臣), Chang R-Z(常汝镇), Qiu L-J(邱丽娟). Genetic contribution of foreign germplasm to elite Chinese soybean (Glycine max) cultivars revealed by SSR markers. Chin Sci Bull (科学通报), 2006, 51(6): 686-692(in Chinese)

[8] Guan R-X(关荣霞), Chang R-Z(常汝镇), Qiu L-J(邱丽娟). Rapid isolation of soybean DNA used for SSR analysis. Soybean Sci (大豆科学), 2003, 22(1): 73-74 (in Chinese with English abstract)

[9] Tasma I M, Lorenzen L L, Green D E, Shoemaker R C. Mapping genetic loci for flowering time, maturity, and photoperiod insensitivity in soybean. Mol Breed, 2001, 8: 25-35

[10] Funatsuki H, Kawaguchi K, Matsuba S,Sato Y, Ishimoto M. Mapping of QTL associated with chilling tolerance during reproductive growth in soybean. Theor Appl Genet, 2005, 142: 137-142

[11] Mansur L M, Orf J H, Chase K, Jarvik T, Cregan P B, Lark K G. Genetic mapping of agronomic traits using recombinant inbred lines of soybean. Crop Sci, 1996, 36: 1327-1336

[12] Lorenzen L L, Lin S F, Shoemaker R C. Soybean pedigree analysis using map-based molecular markers: Recombination during cultivar development. Theor Appl Genet, 1996, 93: 1251-1260

[13] Kimura M. A model of a genetic system which leads to closer linkage by natural selection. Evolution, 1956, 10: 278-287

[14] Nei M. Modification of linkage intensity by natural selection. Genetics, 1967, 57: 625-641

[15] Nei M. Evolutionary change of linkage intensity. Nature, 1968, 218: 1160-1161

[16] Song Q J, Marek L F, Shoemaker R C, Lark K G, Concibido V C, Delannay X, Specht J E, Cregan P B. A new genetic linkage map for soybean. Theor Appl Genet, 2004, 109: 122-128

[17] Ha B K, Bennett J B, Hussey R S, Finnerty S L, Boerma H R. Pedigree analysis of a major QTL conditioning soybean resistance to southern root-knot nematode. Crop Sci, 2004, 44: 758-763

[18] Ha B K, Hussey R S, Boerma H R. Development of SNP assays for marker-assisted selection of two southern root-knot nematode resistance QTL in soybean. Crop Sci, 2007, 47: 573-582

[19] Guo B, Sleper D A, Arrelli P R, Shannon J G, Nguyen H T. Identification of QTLs associated with resistance to soybean cyst nematode races 2, 3 and 5 in soybean PI 90763. Theor Appl Genet, 2005, 103: 1167-1173

[20] Cregan P B, Mudge J, Fickus E W, Danesh D, Denny R, Young N D. Two simple sequence repeat markers to select for soybean cyst nematode resistance conditioned by the rhg1 locus. Theor Appl Genet, 1999, 99: 811-818

[21] Mudge J, Cregan P B, Kenworthy J P, Kenworthy W J,

Orf J H, Young N D. Two microsatellite markers that flank the major soybean cyst nematode resistance locus. Crop Sci, 1997, 37: 1611-1615

[22] Yue P, Arrelli P R, Sleper D A. Molecular characterization of resistance to Heterodera glycines in soybean PI 438489B. Theor Appl Genet, 2001, 102: 921-928

[23] Li Z, Jakkula L, Hussey R S, Tamulonis J P, Boerma H R. SSR mapping and confirmation of the QTL from PI96354 conditioning soybean resistance to southern root-knot nematode. Theor Appl Genet, 2001, 103: 1167-1173

[24] Panthee D R, Pantalone V R, Sams C E, Saxton A M, West D R, Rayford W E. Genomic regions governing soybean seed nitrogen accumulation. J Am Oil Chem Soc, 2004, 81: 77-81

[25] Specht J E, Chase K, Macrander M, Graef G L, Chung J, Markwell J P, Germann M, Orf J H, Lark K G. Soybean response to water: A QTL analysis of drought tolerance. Crop Sci, 2001, 41: 493-509

[26] Liu G-Y(刘广阳). Utilization of elite germplasm Ke4430-20 in soybean breeding in Heilongjiang province. J Plant Genet Resour (植物遗传资源学报), 2005, 6(3): 326-329 (in Chinese with English abstract)

[27] Wang D, Graef G L, Procopiuk A M,Diers B W. Identification of putative QTL that underlie yield in interspecific soybean backcross populations. Theor Appl Genet, 2003, 108: 458-467

[28] Arahana V S, Graef G L, Specht G E, Steadman J R, Eskridge K M. Identification of QTLs for resistance to sclerotinia sclerotiorum in soybean. Crop Sci, 2001, 41: 180-188

[29] Naoki Y, Seishi N, Masako N, Yasutaka T, Masahiro Y, Yashiaki N, Takuji S, Kyuya H. An informative linkage map of soybean reveals QTLs for flowering time, leaflet morphology and regions of segregation distortion. DNA Res, 2001, 8: 61-72

[30] Pahthee D R, Kwanyuen P, Sams C E, West D R, Saxton A M,Pantalone V R.Quantitative trait loci for β-Conglycinin (7S) and glycinin (11S) fractions of soybean storage protein. J Am Oil Chem Soc, 2004, 81: 1005-1012

[31] Orf G H, Chase K, Jarvik T, Mansur L M, Cregan P B, Adler F R, Lark K G. Genetics of soybean agronomic traits: I. Comparison of three related recombinant inbred populations. Crop Sci, 1999, 39: 1642-1651

[32] Romero-Severson J, Smith J S C, Ziegle J, Hauser J, Joe L, Hookstra G. Pedigree analysis and haplotype sharing within diverse groups of Zea mays L. inbreds. Theor Appl Genet, 2001, 103: 567-574

[33] Guan R-X(关荣霞), Liu Y(刘燕), Liu Z-X(刘章雄), Chang R-Z(常汝镇), Qiu L-J(邱丽娟). Purity identification of soybean varieties with SSR technique. Mole Plant Breed (分子植物育种), 2003, 1(3): 357-360 (in Chinese with English abstract)

[34] Meesang N. Ranamukhaarachchi S L, Petersen M J, Andersen S B. Soybean cultivar identification and genetic purity analysis using microsatellite DNA markers. Seed Sci Technol, 2001, 29: 637-645
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