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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (9): 1637-1645.doi: 10.3724/SP.J.1006.2009.01637


Gene Differential Expression at Seedling Stage in Four Cotton Combination Hybridized by CRI-12 and Its Pedigree-Deerived Lines

ZHU Xin-Xia1,2,ZHU Yi-Chao1,AI Ni-Jiang1,LIU Ren-Zhong1,ZHANG Tian-Zhen1*   

  1. 1 National Key Laboratory of Crop Genetics & Germplasm Enhancement / Nanjing Agricultural University, Nanjing 210095, China; 2 Life Science College of Shihezi University, Shihezi 832003, China
  • Received:2008-12-09 Revised:2009-04-24 Online:2009-09-12 Published:2009-07-03
  • Contact: ZHANG Tian-Zhen, E-mail: cotton@njau.edu.cn; Fax: 025-84395307


CRI-12, an Upland cotton variety with high yield, elite fiber quality and disease resistance, is characterized by its high heritability, combining ability and genetic stability. CRI-12 and its pedigree-derived lines were used to develop high heterosis cotton hybrids such as Zhongmiansuo 28, CRI-29, XZM2, and Jimian 18. The roots and leaves at seedling stage of these hybrids and their corresponding parents were sampled for cDNA-AFLP analysis and validation by Quantitative Real-Time PCR. The results were as follows: (1) CIR-12 played a predominant role in the heterosis of vegetative growth in CRI-28, CRI-29, and Jimian18 at seedling stage. (2) Four differential expression types were detected between the hybrid and its parents: I. Up expression only showed in hybrid but not in both parents; II. Dominant expression showed in one of the parents but not in F1 and another parent, including the expression pattern in female parent and hybrid not in male parent, and the expression pattern in male parent and hybrid not in female parent; III. The gene silenced in one of the parents, including the expression pattern in male parent not in hybrid and female parent and the expression pattern in female parent not in hybrid and male parent, IV. Down expression showed in both parents but not in F1. The tendency of proportion in four types was consistent and showed a high ratio in dominant expression and silencing in single parent, but a low ratio in down expression in roots and leaves of hybrids. The type expressed only in one parent and F1 or only in one parent played main role in heterosis. Differential expression genes in leaves and roots accounted for 29.20–46.09% and 15.65–22.49%, respectively. The differential expression genes in leaves were more than those in roots, indicating that some genes play larger roles for heterosis than these in roots. (3) Genes co-expressed between hybrids and high value parent CRI-12 and/or its derived lines were much more than those between hybrids and the relative low value parents, indicating that CIR-12 plays a predominant role in the expression of genes responsible for heterosis in CRI-28, CRI-29, and Jimian18 at seedling stage. (4) Differentially expressed genes in the four hybrids accounted for 27.00–34.56% of total genes detected. Further analysis revealed that the main modes of gene action involved in hybrids were additive and dominant effects accounting for 79.52–83.79%, and the effect of over-dominance accounting for 3.30–7.17%, under-dominance effects accounting for 2.62–4.14% and low-parent dominance effects accounting for 5.65–13.03%. All possible modes of gene action co-existed supported the hypothesis of multiple gene mechanisms contributed to heterosis. (5) Over-dominance effect in CRI-28 which express high parent heterotic is 7.17%, higher than that in three other middle heterotic crosses at three earlier growing stages, which suggesting the differential expression type expressed only in F1 play an important role in heterosis of vegetative growth.

Key words: CRI-12, Hybrid Cotton, Differential Expression gene

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