水稻新型卷叶突变体rl12（t）的遗传分析和基因定位

doi:10.3724/SP.J.1006.2009.01967

Abstract

Abstract:

Leaf is an important organ in photosynthesis, its moderate rolling could facilitate the improvement of plant population’s structure and enhancelight-use efficiency, which is significant in ideotype breeding. Recently, more attention has been paid to the identification of rice rolled leaf mutants and related gene cloning by breeders and geneticists. This paper reported a rolled leaf mutant, temporarily named rl12(t), from the restorer line Jinhui10 treated by EMS. In the mutant, the initializing leaves didn’t roll, the mature leaves curled the upper 1/3 section of them and the older mature leaves rolled completely, the pigment contents increased significantly.To date, such phenotype has never been reported. One CMS line Xinong 1A with normal leaves was crossed with the rl12(t) mutant, the F₁/F₂ populations were used for genetic analysis, suggesting that the mutant trait were controlled completely by one single dominant nuclear gene. rl12(t) was finally located on the chromosome 10 between SWU-1 and SWU-2 with genetic distances of 1.5 and 0.2 cM, respectively. There has been no rolled leaf gene reported on this chromosome. So the RL12(t) should be novel, and also the unique dominant rolled leaf gene mapped by molecular markers up to now. The results provide a basis for RL12(t) gene cloning and functional analysis, as well as mechanism studies of rolled leaf and the application in plant-type breeding.

Key words: Oryza sattiva, Dominant rolled leaf gene, Genetic analysis, Gene mapping

LUO Yuan-Zhang,ZHAO Fang-Ming,SANG Xian-Chun,LING Ying-Hua,YANG Zheng-Lin. Genetic Analysis and Gene Mapping of a Novel Rolled Leaf Mutant rl12(t) in Rice[J].Acta Agron Sin, 2009, 35(11): 1967-1972.

References

[1] Yuan L-P(袁隆平). Hybrid rice breeding for super high yield. Hybrid Rice (杂交水稻), 1997, 12(6): 1-6 (in Chinese with English abstract)

[2] Shen F-C(沈福成). Several opinions on how to use rolled leaf character of rice in breeding. J Guizhou Agric Sci (贵州农业科学), 1983, (5): 6-8 (in Chinese with English abstract)

[3] Chen Z-X(陈宗祥), Pan X-B(潘学彪), Hu J(胡俊). Relationship between rolled leaf and ideal plant type of rice (Oryza sativa L.). J Jiangsu Agric Res (江苏农业研究), 2001, 22(4): 88-91 (in Chinese with English abstract)

[4] Chen Z-X(陈宗祥), Chen G(陈刚), Pan X-B(潘学彪). Genetic expression and effects of rolled leaf gene Rl(t) in hybrid rice(Oryza sativa). Acta Agron Sin (作物学报), 2002, 28(6): 847-851 (in Chinese with English abstract)

[5] Lang Y-Z(郎有忠), Zhang Z-J(张祖建), Gu X-Y(顾兴友), Yang J-C(杨建昌), Zhu Q-S(朱庆森). Physiological and ecological effects of crimpy leaf character in rice (Oryza sativa L.): I. Leaf orientation, canopy structure and light distribution. Acta Agron Sin (作物学报), 2004, 30(9): 883-887 (in Chinese with English abstract)

[6] Lang Y-Z(郎有忠), Zhang Z-J(张祖建), Gu X-Y(顾兴友), Yang J-C(杨建昌), Zhu Q-S(朱庆森). Physiological and ecological effects of crimpy leaf character in rice (Oryza sativa L.): II. Photosynthetic character, dry mass production and yield forming. Acta Agron Sin (作物学报), 2004, 30(8): 806-810 (in Chinese with English abstract)

[7] Yu D(余东), Wu H-B(吴海滨), Yang W-T(杨文韬), Gong P-T(巩鹏涛), Li Y-Z(李有志), Zhao D-G(赵德刚). Genetic analysis and mapping of the unilateral rolled leaf trait of rice mutant B157. Mol Plant Breed (分子植物育种), 2008, 6(2): 220-226 (in Chinese with English abstract)

[8] Li S-G(李仕贵), Ma Y-Q(马玉清), He P(何平), Li H-Y(黎汉云), Chen Y(陈英), Zhou K-D(周开达), Zhu L-H(朱立煌). Genetic analysis and mapping the flag leaf roll in rice (Oryza sativa L.). J Sichuan Agric Univ (四川大学学报), 1998, 16(4): 391-393 (in Chinese with English abstract)

[9] Shao Y-J(邵元健), Chen Z-X(陈宗祥), Zhang Y-F(张亚芳), Chen E-H(陈恩会), Qi D-C(祁顶成), Liao J(缪进), Pan X-B(潘学彪). One major QTL mapping and physical map construction for rolled leaf in rice. Acta Genet Sin (遗传学报), 2005, 32(5): 501-506 (in Chinese with English abstract)

[10] Yan C J, Yan S, Zhang Z Q, Liang G H, Lu J F, Gu M H. Genetic analysis and gene fine mapping for a rice novel mutant (rl9_(t)) with rolling leaf character. Chin Sci Bull, 2006, 51(1): 63-69

[11] Luo Z K, Yang Z L, Zhong B Q, Li Y F, Xie R, Zhao F M, Ling Y H, He G H. Genetic analysis and fine mapping of a dynamic rolled leaf gene, RL10(t), in rice (Oryza sativa L.). Genome, 2007, 50: 811-817

[12] Shi Y-F(施勇烽), Chen J(陈洁), Liu W-Q(刘文强), Huang Q-N(黄奇娜), Shen B(沈波), Leung H, Wu J-L(吴建利). Genetic analysis and gene mapping of a novel rolled leaf mutant in rice (Oryza sativa L.). Sci China (Ser C)(中国科学·C辑), 2009, 39(4): 407-412 (in Chinese)

[13] Xia L(夏令), Chen L(陈亮), Guo C-M(郭迟鸣), Zhang H-X(张红心), Zhao Z(赵政), Shen M-S(沈明山), Chen L(陈亮). Genetic analysis and mapping of rice (Oryza sativa L.) sd-sl mutant. J Xiamen Univ (Nat Sci) (厦门大学学报·自然科学版), 2007, 46(6): 847-851 (in Chinese with English abstract)

[14] Wang D K,Liu H Q, Li K L, Li S J, Tao Y Z. Genetic analysis and gene mapping of a narrow leaf mutant in rice (Oryza sativa L.). Chin Sci Bull, 2009, 54(5): 752-758

[15] Shao Y J, Pan C H, Chen Z X, Zuo S M, Zhang Y F, Pan X B. Fine mapping of an incomplete recessive gene for leaf rolling in rice (Oryza sativa L.). Chin Sci Bull, 2005, 50(21): 2466-2472

[16] Shen G-Z(沈革志), Wang X-Q(王新其), Yin L-Q(殷丽青), Wang J(王江), Li L(李琳), Zhang J-L(张景六). Genetic analysis of a rolled-leaf mutant in rice population of T-DNA insertion. Acta Biol Exp Sin (实验生物学报), 2003, 36(6): 459-464(in Chinese with English abstract)

[17] Chen Z-G(陈兆贵), Wang J(王江), Zhang Z-M(张泽民), Liu F(刘芳), Zhu H-T(朱海涛), Wang X-S(宛新杉), Zhang J-L(张景六), Zhang G-Q(张桂权). Genetic analysis of a rolled leaf mutation by T-DNA (Ds) insertion in Oryza sativa. J South China Agric Univ (华南农业大学学报), 2006, 27(1): 1-4 (in Chinese with English abstract)

[18] Zou Q(邹琦). Experimental Guide for Plant Physiology (植物生理学实验指导). Beijing: China Agriculture Press, 2000. pp 72-75 (in Chinese)

[19] Rogers S O, Bendich A J. Extraction of DNA from plant tissues. Plant Mol Biol Manual, 1988, A6: 1-10

[20] Sang X-C(桑贤春), He G-H(何光华), Zhang Y(张毅), Yang Z-L(杨正林), Pei Y(裴炎). The simple gain of templates of rice genomes DNA for PCR. Hereditas(遗传), 2003, 25(6): 705-707 (in Chinese with English abstract)

[21] Panaud O, Chen X, McCouch S R. Development of microsatellite markers and characterization of simple sequence length polymorphism (SSLPs) in rice (Oryza sativa L.). Mol Gen Genet, 1996, 252: 597-607

[22] Lander E S, Green P, Abrahamson J, Barlow A, Daly M J, Lincoln S E, Newburg L. MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics, 1987, 1: 174-181

[23] Kosambi D D. The estimation of map distances from recombination values. Ann Eugen, 1944, 12: 172-175

[24] Erik H M, Chen Y Z, Hubbart S, Peng S B, Horton P. Interaction between senescence and leaf orientation determine in situpatterns of photosynthesis and photoinhibition in field-grown rice. Plant Physiol,1999, 119: 553-563

[25] Peter H. Prospects for crop improvement through the genetic manipulation of photosynthesis: morphological and biochemical aspects of light capture. J Exp Bot, 2000, 51: 475-485

[26] Sinclair T R, Sheehy J E. Erect leaves and photosynthesis in rice. Science, 1999, 283: 1456-1457

[27] Meng J(孟军), Chen W-F(陈温福), Xu Z-J(徐正进), Li L-X(李磊鑫), Zhou S-Q(周淑清). Study on photosynthetic rate and chlorophyll content. J Shenyang Agric Univ(沈阳农业大学学报), 2001, 32(4): 247-249(in Chinese with English abstract)

[28] Ou Z-Y(欧志英), Peng C-L(彭长连), Yang C-W(阳成伟), Lin G-Z(林桂珠), Duan J(段俊), Wen X(温学). High efficiency photosynthetic characteristics in flag leaves of super high-yielding rice. J Trop Subtrop Bot (热带亚热带植物学报), 2003, 11(1): 1-6 (in Chinese with English abstract)

[29] Yan S, Yan C J, Zeng X H, Yang Y C, Fang Y W, Tian C Y, Sun Y W, Cheng Z K, Gu M H. Rolled leaf 9, encoding a GARP protein, regulates the leaf abaxial cell fate in rice. Plant Mol Biol, 2008, 68: 239-250

[30] Zhang G H, Xu Q, Zhu X D, Qian Q, Xue H W. SHALLOT-LIKE1 is a KANADI transcription factor that modulates rice leaf rolling by regulating leaf Abaxial cell development. Plant Cell, 2009, 21: 719-735

[31] Deng J-X(邓加省), Yu X-Q(余显权), Wang J(王锦), Gao G-Z(高贵忠). Genetic analysis of the leaf rolling characteristics the GuiNongTongHui. J Swau Agric Sci (西南农业学报), 2006, 19(6): 1005-1008 (in Chinese with English abstract)

[32] Gao Y-H(高艳红), Lü C-G(吕川根), Wang M-Q(王茂青), Wang P(王澎), Yan X-Y(闫晓燕), Xie K(谢坤), Wan J-M(万建民). QTL mapping for rolled leaf gene in rice. J Jiangsu Agric Sci (江苏农业学报), 2007, 23(1): 5-10 (in Chinese with English abstract)

[33] Micol L J, Hake S. The development of plant leaves. Plant Physiol, 2003, 131: 389-394

[34] Byrne M, Timmermans M, Kidner C, Martienssen R. Development of leaf shape. Curr Opin Plant Biol, 2001, 4: 38-43

[35] Talbert P B, Adler H T, Parks D W, Comai L. The REVOLUTA gene is necessary for apical meristem development and for limiting cell divisions in the leaves and stems of Arabidopsis thaliana. Genes Dev, 1995, 121: 2723-2735

[36] Ratcliffe O J, Riechmann J L, Zhang J Z. INTERFASCICULARF IBERLESS1 is the same gene as REVOLUTA. Plant Cell, 2000, 12: 315-317

[37] McConnell J R, Emery J, Eshed Y, Bao Y, Bowman J, Barton M K. Role of PHABULOSA and PHAVOLUTA in determining radial patterning in shoots. Nature, 2001, 411: 709-713

[38] Juarez M T, Kui J S, Thomas J, Heller B A, Timmermans M C. MicroRNA-mediated repression of rolled leaf1 specifies maize leaf polarity. Nature, 2004, 428: 84-88

[39] Mallory A C, Reinhart B J, Jones-Rhoades M W, Tang G, Zamore P D, Barton M K, Bartel D P. microRNA control of PHABULOSA in leaf development: Importance of pairing to the microRNA 5' region. EMBO J, 2004, 23: 3356-3364

[40] Vaucheret H, Vazquez F,Crété P, Bartel D P. The action of ARGONAUTE1 in the miRNA pathway and its regulation by the miRNA path-way are crucial for plant development. Genes Dev, 2004, 18: 1187-1197
Fujino K, Matsuda Y, Ozawa K, Nishimura T, Koshiba T, Fraaije M W, Sekiguchi H. NARROW LEAF 7 controls leaf shape mediated by auxin in rice. Mol Genet Genomics, 2008, 279: 499-507

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Genetic Analysis and Gene Mapping of a Novel Rolled Leaf Mutant rl12(t) in Rice

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