花生中DREB类转录因子PNDREB1的克隆及鉴定

doi:10.3724/SP.J.1006.2009.01973

Abstract

Abstract:

The dehydration responsive element binding proteins (DREB) are important and specific plant transcription factors responding to stress conditions including drought, salt and low temperature.It has been generally accepted that DREB can regulates the expression of a number of abiotic stress-related genes in down stream of the stress signal transduction pathways. In this paper, a DREB-like gene, named PNDREB1 (Accession No. FM955398), was cloned by screening a peanut (Arachis hypogaea L.) full-length cDNA library of immature seeds. The structure analysis showed that PNDREB1 contained a 687 bp ORF, encoding a protein of 229 amino acids with predicted molecular weight of 24.7 kD and a isoelectric point of 5.97. The predicted protein sequence contained one conserved AP2 domain, which is the typical characteristic of DREB transcription factors. Based on the sequences similarity, PNDREB1 is classified into A-1 subgroup of DREB subfamily. Furthermore, the yeast hybrid system was carried out, and the results confirmed that the AP2 domain of PNDREB1 could specifically interact with DRE cis-acting element. The activation activity of the C-terminal end as a transcriptional activator was also been proved experimentally. The expression pattern analysis carried out by semi-quantitative RT-PCR indicated that PNDREB1 was constitutively expressed in various tissues of peanut, and was strongly upregulate by treatments with low temperature, also respond to dehydration. However, the expression of PNDREB1 was not affected by high salinity and exogenous application of abscisic acid (ABA). In this study, we isolated and characterized a novel peanut DREB-like transcription factor which was regulated by low temperature and osmotic stresses.

Key words: Peanut, DREB Transcription factor, PNDREB1, Yeast hybrid system, Expression pattern, Low temperature, Dehydration

ZHANG Mei,LIU Wei,BI Yu-Ping,WANG Zi-Zhang. Isolation and Identification of pndreb1-A New DREB Transcription Factor from Peanut（Arachis hypogaea L.）[J].Acta Agron Sin, 2009, 35(11): 1973-1980.

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Isolation and Identification of pndreb1-A New DREB Transcription Factor from Peanut（Arachis hypogaea L.）

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