水稻新型卷叶突变体rl12（t）的遗传分析和基因定位

doi:10.3724/SP.J.1006.2009.01967

Abstract

Abstract:

Leaf is an important organ in photosynthesis, its moderate rolling could facilitate the improvement of plant population’s structure and enhancelight-use efficiency, which is significant in ideotype breeding. Recently, more attention has been paid to the identification of rice rolled leaf mutants and related gene cloning by breeders and geneticists. This paper reported a rolled leaf mutant, temporarily named rl12(t), from the restorer line Jinhui10 treated by EMS. In the mutant, the initializing leaves didn’t roll, the mature leaves curled the upper 1/3 section of them and the older mature leaves rolled completely, the pigment contents increased significantly.To date, such phenotype has never been reported. One CMS line Xinong 1A with normal leaves was crossed with the rl12(t) mutant, the F₁/F₂ populations were used for genetic analysis, suggesting that the mutant trait were controlled completely by one single dominant nuclear gene. rl12(t) was finally located on the chromosome 10 between SWU-1 and SWU-2 with genetic distances of 1.5 and 0.2 cM, respectively. There has been no rolled leaf gene reported on this chromosome. So the RL12(t) should be novel, and also the unique dominant rolled leaf gene mapped by molecular markers up to now. The results provide a basis for RL12(t) gene cloning and functional analysis, as well as mechanism studies of rolled leaf and the application in plant-type breeding.

Key words: Oryza sattiva, Dominant rolled leaf gene, Genetic analysis, Gene mapping

LUO Yuan-Zhang,ZHAO Fang-Ming,SANG Xian-Chun,LING Ying-Hua,YANG Zheng-Lin. Genetic Analysis and Gene Mapping of a Novel Rolled Leaf Mutant rl12(t) in Rice[J].Acta Agron Sin, 2009, 35(11): 1967-1972.

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Genetic Analysis and Gene Mapping of a Novel Rolled Leaf Mutant rl12(t) in Rice

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