花生中DREB类转录因子PNDREB1的克隆及鉴定

doi:10.3724/SP.J.1006.2009.01973

Abstract

Abstract:

The dehydration responsive element binding proteins (DREB) are important and specific plant transcription factors responding to stress conditions including drought, salt and low temperature.It has been generally accepted that DREB can regulates the expression of a number of abiotic stress-related genes in down stream of the stress signal transduction pathways. In this paper, a DREB-like gene, named PNDREB1 (Accession No. FM955398), was cloned by screening a peanut (Arachis hypogaea L.) full-length cDNA library of immature seeds. The structure analysis showed that PNDREB1 contained a 687 bp ORF, encoding a protein of 229 amino acids with predicted molecular weight of 24.7 kD and a isoelectric point of 5.97. The predicted protein sequence contained one conserved AP2 domain, which is the typical characteristic of DREB transcription factors. Based on the sequences similarity, PNDREB1 is classified into A-1 subgroup of DREB subfamily. Furthermore, the yeast hybrid system was carried out, and the results confirmed that the AP2 domain of PNDREB1 could specifically interact with DRE cis-acting element. The activation activity of the C-terminal end as a transcriptional activator was also been proved experimentally. The expression pattern analysis carried out by semi-quantitative RT-PCR indicated that PNDREB1 was constitutively expressed in various tissues of peanut, and was strongly upregulate by treatments with low temperature, also respond to dehydration. However, the expression of PNDREB1 was not affected by high salinity and exogenous application of abscisic acid (ABA). In this study, we isolated and characterized a novel peanut DREB-like transcription factor which was regulated by low temperature and osmotic stresses.

Key words: Peanut, DREB Transcription factor, PNDREB1, Yeast hybrid system, Expression pattern, Low temperature, Dehydration

ZHANG Mei,LIU Wei,BI Yu-Ping,WANG Zi-Zhang. Isolation and Identification of pndreb1-A New DREB Transcription Factor from Peanut（Arachis hypogaea L.）[J].Acta Agron Sin, 2009, 35(11): 1973-1980.

References

[1] Okamuro J K, Caster B, Villarroel R. The AP2 domain of APETALA2 define a large new family of DNA binding protein in Arabidopsis. Proc Natl Acad Sci USA, 1997, 94: 7076-7081

[2] Liu Q, Kasuga M, Sakuma Y, Abe H, Miura S, Yamaguchi-Shinozaki K, Shinozaki K. Two transcription factors, DREB1 and DREB2, with an EREBP/AP2 DNA binding domain separate two cellular signal transduction pathways in drought- and low-temperature-responsive gene expression, respectively, in Arabidopsis. Plant Cell, 1998, 10: 1391-1406

[3] Yamaguchi-Shinozakiaib K, Shinozaki K. A Novel cis-acting element in an Arabidopsis gene is involved in responsiveness to drought, low temperature, or high-salt stress. Plant Cell, 1994, 6: 251-264

[4] Stockinger E J, Gilmour S J, Thomashow M F. Arabidopsis thaliana CBF1 encodes an AP2 domain-containing transcriptional activator that binds to the C-repeat/DRE, a cis-acting DNA regulatory element that stimulates transcription in response to low temperature and water deficit. Proc Natl Acad Sci USA, 1997, 94: 1035-1040
[5] Zhang M(张梅), Liu W(刘炜), Bi Y-P(毕玉平). Dehydration-responsive element-binding (DREB) transcription factor in plants and its role during abiotic stresses. Hereditas(遗传), 2009, 31(3): 236-244(in Chinese with English abstract)

[6] Hsieh T S, Lee J T, Yang P T, Chiu L H, Charng Y Y, Wang Y C, Chan M T. Heterology expression of the Arabidopsis C-repeat/dehydration response element binding factor1 gene confers elevated tolerance to chilling and oxidative stresses in transgenic tomato. Plant Physiol, 2002, 129: 1086-1094

[7] Dubouzet J G, Sakuma Y, Ito Y, Kasuga M, Dubouzet E G, Miura S, Seki M, Shinozaki K, Yamaguchi-Shinozaki K. OsDREB genes in rice, Oryza sativa L., encode transcription activators that function in drought-, high-salt- and cold-responsive gene expression. Plant J, 2003, 33: 751-763

[8] Sakuma Y, Maruyama K, Qin F, Osakabe Y, Shinozaki K, Yamaguchi-Shinozaki K. Dual function of an Arabidopsis transcription factor DREB2A in water-stress-responsive and heat-stress-responsive gene expression. Proc Natl Acad Sci USA, 2006, 103: 18822-18827
[9] Sun S, Yu J P, Chen F, Zhao T J, Fang X H, Li Y Q, Sui S F. Tiny, a DREB-like transcription factor connecting the DRE- and ERE-mediated signaling pathways in Arabidopsis. J Biol Chem., 2008, 283: 6261-6271
[10] Carra A, Gambino G, Schubert A. A cetyltrimethylammonium bromide-based method to extract low-molecular-weight RNA from polysaccharide-rich plant tissues. Anal Biochem, 2007, 360: 318-320

[11] Chen Y-Q(陈由强), Ye B-Y(叶冰莹), Zhu J-M(朱锦懋), Zhuang W-J(庄伟建), Pan D-R(潘大仁), Chen R-K(陈如凯). A simple and modified procedure to isolate total DNA from leaves of peanut (Arachis hypogaea). Peanut Sci Technol(花生科技), 1999, (3): 1-4 (in Chinese with English abstract)
[12] Wang P-R(王平荣), Deng X-J(邓晓建), Gao X-L(高晓玲), Chen J(陈静), Wan J(万佳), Jiang H(姜华), Xu Z-J(徐正君). Progress in the study on DREB transcription factor. Hereditas (遗传), 2006, 28(3): 369-374 (in Chinese with English abstract)
[13] Sakuma Y, Liu Q, Dubouzet J G, Abe H, Shinozaki K, Yamaguchi-Shinozaki K. DNA-binding specificity of the ERF/AP2 domain of Arabidopsis DREBs, transcription factors involved in dehydration- and cold-inducible gene expression. Biochem Biophys Res Commun, 2002, 290: 998-1009
[14] Li J J, Herskowitz I. Isolation of ORC6, a component of the yeast origin recognition complex by a one hybrid system. Science, 1993, 262: 1870-1874

[15] Hu Z-L(胡振林), Sun S-H(孙树汉), Da J-X(戴建新), Zhou F-J(周凤娟). Screening out CpG immunostimulatory sequence-specific DNA-binding proteins with yeast-one-hybrid system. Acad J Second Military Med Univ (第二军医大学学报), 200l, 22(6): 542-545(in Chinese with English abstract)

[16] Jaglo K R, Kleff S, Amundsen K L, Zhang X, Haake V, Zhang J Z, Deits T, Thomashow M F. Components of the Arabidopsis C-repeat/dehydration-responsive element binding factor cold-response pathway are conserved in Brassica napus and other plant species. Plant Physiol, 2001, 127: 910-917
[17] Chen M, Wang Q Y, Cheng X G, Xu Z S, Li L C, Ye X G, Xia L Q, Ma Y Z. GmDREB2, a soybean DRE-binding transcription factor, conferred drought and high-salt tolerance in transgenic plants. Biochem Biophys Res Commun,2007, 353: 299-305

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Isolation and Identification of pndreb1-A New DREB Transcription Factor from Peanut（Arachis hypogaea L.）

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