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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (12): 2187-2196.doi: 10.3724/SP.J.1006.2009.02187

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Effects Contributed by Different Donor Parents and Backcross Times on R08 Improvement

WANG Yu-Hua,QIAO Shan-Bao,YANG Ke-Cheng*,RONG Ting-Zhao,PAN Guang-Tang,GAO Shi-Bin*   

  1. Maize Research Institute,Sichuan Agricultural University/Key Laboratory of Crop Genetic Resources and Improvement,Ministry of Education,Ya'an 625014,China
  • Received:2009-04-27 Revised:2009-08-22 Online:2009-12-10 Published:2009-10-13
  • Contact: YANG Ke-Chen,Tel:0835-2882465;GAO Shi-Bin,E-mail:shibingao@gmail.com

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Abstract:

In this study, we usedR08 as the recurrent parent and 18 excellentinbreeding lines as the donor parents to improve R08 line through backcross and self-cross. A total of 18 BC1F3-derived lines and 18 BC2F2-derived lines with relatively diverse genetic background were obtained. Improving effects contributed by different donor parents and backcross times on R08 were analyzed by evaluation field trials, combining ability and SSR molecular markers of R08 improved lines. The results showed that 29 lines out of 36 BC-derived lines were resistant or high-resistant to northern leaf blight. General combining ability (GCA)of most yield traits in the majority of improved lines showed no decrease or a little increase compared with that of R08. BC1F3 (backcross once, self-cross twice) wasbetter than BC2F2 (backcross twice, self-cross once) in the improvement of disease resistance to northern leaf blightcompared with the lines which had same donor parents and different times of backcross, while the GCA of most yield traits in majority of the lines selected from the improved offspring was roughly the same. The results of SSR markers analysis showed that the genetic variation in the lines had no significant difference. On the contrary, there was a larger difference in disease resistance to northern leaf blight, GCA of the majority of yield traits and the results of SSR markersanalysis in the lines selected from different donor and same times of backcross. Donor parents Chang 7-2 and Chuan 321 played a larger role in improving R08’s disease resistance to northern leaf blight and most of yield traits’ GCA. Therefore, they are excellent donor parents. As a result, w4-1 and w10-1 are selected as elite lines of backcross-improving. Consequently, during the utilization of backcross in improving maize inbred lines, we could backcross once on the basis of selecting the right donor parents, and strengthen the identification and selection of target traits and the determination of GCA in self-cross process to improve the breeding efficiency of backcross improvement.

Key words: Corn, Donor parent, Backcross breeding, Combining ability, SSR


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