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Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (2): 228-232.doi: 10.3724/SP.J.1006.2010.00228

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Transgenic Brassica napus Growing with Phytate as a Sole Phosphorus Source

FANG Xiao-Ping1,WANG Zhuan1,CHEN Ru-Mei2,LI Jun1,FAN Yun-Liu2,LUO Li-Xia1,CHEN Kun-Ron1,REN Li1   

  1. 1 Key Laboratory of Oil Crops Genetic Improvement, Ministry of Agriculture / Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, China; 2 Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2009-07-14 Revised:2009-10-02 Online:2010-02-10 Published:2009-12-21
  • Contact: xpfang2008@163.com; xpfang@public.wh.hb.cn

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Abstract:

Phosphorus (P) deficiency in soil is a major constraint in agricultural production worldwide. Most soils contain significant amounts of total soil P that occurs in insoluble inorganic and organic fractions, but lack available phosphorus. Phytic acid is the major storage form of organic phosphorus in soil. In this experiment, the phytase gene with the signal peptide sequence of extracellular secretion was introduced into Brassica napus cv. Zhongshuang 4 via Agrobacterium tumefaciens LBA4404. Fifty six plants of transgenic Brassica napus were obtained and checked by PCR and phytase activity, most of them gave the positive results. The transformation efficiency was 0.16–9.2%. When grown in MS medium with phytic acid as a sole phosphorus source under sterile conditions, transgenic Brassica napus plants were able to obtain inorganic phosphate from phytic acid and grew normally, but the wild-type plants not. These results show that extracellular phytase secreted from plant roots is a significant factor in the utilization of phosphorus from phytate and indicate that there exists a prospect for using gene technology to improve the ability of plants to utilize accumulated forms of soil organic phosphorus.

Key words: Brassica napus, Phytase, Transgene, Extracellular secretion

[1] Atkinson D. Some general effects of phosphorus deficiency on growth and development. New Phytol, 1973, 72: 101-111

[2] Institute of Soil Science of Chinese Academy of Sciences (中国科学院南京土壤研究所主编). Chinese Soil (中国土壤). Beijing: Science Press, 1978. pp 376-391(in Chinese)

[3] Bielesk R L.Phosphate pools, phosphate transport, and phosphate availability. Annu Rev Plant Physiol, 1973, 24: 225-252

[4] Dalal R C. Soil organic phosphorus. Adv Agron, 1977, 29: 83-117

[5] Anderson G. Assessing organic phosphorus in soil. In: Khasawneh F E, Sample E C Kamprath E J, eds. The Role of Phosphorus in Agriculture. Madison, USA: Amer. Soc. Agron. 1980. pp 411-432

[6] Turner B L, Mahieu N, Condron L M. Quantification of myoinositol hexaphosphate in alkaline soil extracts by solution 31P-NMR spectroscopy and spectral deconvolution. Soil Sci, 2003, 168: 469-478

[7] Turner B L, Papha´zy M J, Haygarth P M, McKelvie I D. Inositol phosphates in the environment. Philo Rans Royal Soc, Series B, 2002, 357: 449-469

[8] Raboy V. Progress in breeding low phytate crops. -505J Nutr, 2002, 132: 503

[9] Yao B(姚斌), Fan Y-L(范云六). Molecular biology and gene engineering of phytase. Chin J Biotechnol生物工程学报), 2000, 16: 1-5 (in Chinese with English abstract) (

[10] Reddy N R, Sathe S K. Occurrence, Distribution, Content and Dietary Intake of Phytate. In: Food Phytates. Boca Raton: CRC press, 2002. pp 25-52

[11] Sun H-G(孙海国), Zhang F-S(张福锁). Effect of phosphorus deficiency on activity of acid phosphates exuded by wheat roots. Chin J Appl Ecol (应用生态学报), 2002, 13(3): 379-381 (in Chinese with English abstract)

[12] Irving G C J. Phytase. In: Cosgrove D J ed. Inositol Phosphates: Their Chemistry, Biochemistry and Physiology (Studies in Organic Chemistry 4). 1980. pp 85-186

[13] Hayes J E, Richardson A E, Simpson R J. Components of organic phosphorus in soil extracts that are hydrolysed by phytase and acid phosphatase. Biol Fertil Soils, 2000, 32: 279-286

[14] Hayes J E, Richardson A E, Simpson R J. Phytase and acid phosphatase activities in extracts from roots of temperate pasture grass and legume seedlings. Aust J Plant Physiol, 1999, 26: 801-809

[15] Richardson A E, George T S, Hens M, Simpson R J. Utilisation of soil organic phosphorus by higher plants. In: Turner B L, Frossard E, Baldwin D, eds. Organic Phosphorus in the Environment. Wallingford: CABI Publishing. 2004. pp 165-184

[16] Kong F-L(孔凡利), Ling W-L(林文量), Yan X-L(严小龙), Liao H(廖红). Phytate-phosphorus uptake and utilization by transgenic tobacco carrying Bacillus subtilis phytase gene. Chin J Appl Ecol (应用生态学报), 2005, 16(12): 2225-2478 (in Chinese with English abstract)

[17] Zou L-K邹立扣)，Wang H-N王红宁). Phytase and its plant genetic engineering. (((Microbiology微生物学通报), 2005, 32(6): 128-132 (in Chinese with English abstract)

[18] Chan W L, Lung S C, Lim B L.-106 Properties of beta-propeller phytase expressed in transgenic tobacco. Protein Exp Purif, 2006, 46: 100

[19] Shen Y-O(沈亚欧), Peng H-W(彭焕伟), Pan G-T(潘光堂). Research study on expressed phytase in transgenic plants. Chin J Biotechnol (中国生物工程杂), 2005, 25(1): 29-32 (in Chinese with English abstract)

[20] Xiao K, Harrison M J, Wang Z Y.-36 Transgenic expression of a novel M. truncatula phytase gene results in improved acquisition of organic phosphorus by Arabidopsis. Planta, 2005, 222: 27

[21] Hong C Y, Cheng K J, Tseng T H, Wang C S, Liu L F, Yu S M.-39 Production of two highly active bacterial phytases with broad pH optima in germinated transgenic rice seeds. Transgenic Res, 2004, 13: 29

[22] Richardson A E, Hadobas P A, Hayes J E. Extracellular secretion of Aspergillus phytase from Arabidopsis roots enables plants to obtain phosphorus from phytate. Plant J, 2001, 25: 641-649

[23] Mudge S R, Smith F W, Richardson A E. Root-specific and phosphate-regulated expression of phytase under the control of a phosphate transporter promoter enables Arabidopsis to grow on phytate as a sole P source. Plant Sci, 2003, 165: 871-878

[24] Yan X-L(阎秀兰), Duan H-Y(段海燕), Wang Y-H(王运华). Phosphorus efficiency of different rape (Brassica napus L.) genotypes. Chin J Oil Crop Sci(中国油料作物学报), 2002, 24(2): 47-49 (in Chinese with English abstract)

[25] Ponstein A S, Bade J B, Verwoerd T C, MolendijkStable expression of Phytase (phyA) in canola (Brassica napus) seeds: Towards a commercial product. Mol Breed, 2002, 10: 31-44 L, Storms J, Beudeker R F and Pen J.

[26] Murray M G, Thompson W F. Rapid iso1ation of high mo1ecular weight plant.DNA Nucl Acid Res, 1980, 8: 4321-4325

[27] Sambrook J, Fritsch E F, Maniatis T. Molecular Cloning: A Laboratory Manual. New York: Cold Spring Harbor Laboratory Press, 2002

[28] Yan X-P(闫新甫). Transgenic Plants (转基因植物). Beijing: Science Press, 2003 (in Chinese)

[29] Moloney M M, Walker J M, Sharma K K. High efficiency transformation of Brassica napus using Agrobacterium vectors. Plant Cell Rep, 1989, 8: 238-242

[30] Xie J-K(谢建坤), Xiong H-J(熊焕金), Wan Y(万勇), Xiao Y-Q(肖叶青). Advances in plant gene transformation techniques of Brassica mediated by Agrobacterium tumefaciens. Acta Agric Jiangxi (江西农业学报), 2002, 14(2): 38-44 (in Chinese with English abstract)
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