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Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (2): 242-248.doi: 10.3724/SP.J.1006.2010.00242

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

QTL Analysis for Dynamic Expression of Chlorophyll Content in Soybean (Glycine max L. Merri.)

LI Guang-Jun1,2,LI He-Nan,CHENG Li-Guo,ZHANG Yuan-Ming1,*   

  1. 1 State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University / National Center for Soybean Improvement, Nanjing 210095, China; 2 Linyi Normal University, Linyi 276005, China
  • Received:2009-07-28 Revised:2009-12-08 Online:2010-02-10 Published:2009-12-21
  • Contact: ZHANG Yuan-Ming，E-mail:soyzhang@njau.edu.cn

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Abstract:

Chlorophyll is the most important photosynthetic pigment and closely related to soybean seed yield. However, there are still very few studies at different developmental stages under multiple environments. A genetic linkage map using 244 F2 plants derived from a soybean cross between Lishuizhongzihuang and Nannong 493-1 was constructed. F2:3 and F2:4 families were used to dissect the developmental behavior for chlorophyll content across different environments by quantitative trait locus (QTL) mapping approach. Chlorophyll contentsfor the function leaf (in vivo) in the 244 F2:3 and F2:4 families from seedling to blooming stages at Jiangpu and/or Linyi experimental stations in 2007 and/or 2008 were measured by SPAD-502 chlorophyll instrument. Composite interval mapping (CIM) of Windows QTL Cartographer V2.5 was used for the QTL analysis. A total of 20 QTLs were detected at various developmental stages under two environments, but there were few common QTLs identified across different developmental stages, across years and across experimental stations, although there were 3 to 4 QTLs detected on each of the N, D1a, F and K linkage groups. In the two years above, one common QTL, qchl-D1a-1, located between markers sat_160 and satt147 on the linkage group D1a, was identified at Jiangpu experimental station. At the two experimental stations above, three common QTLs on the K, M and N linkage groups were mapped. These results provide a theoretical basis for genetic analysis of chlorophyll traits and marker-assisted breeding.

Key words: Soybean, Chlorphyll, Quantitative trait locus, Dynamic Expression


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