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Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (2): 256-260.doi: 10.3724/SP.J.1006.2010.00256

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

QTL Identification for Plant Height in a New Dwarf Germplasm of Maize

SHI Yun-Su,YU Yong-Tao,SONG Yan-Chun,LIU Zhi-Zhai, LI Yu,WANG Tian-Yu   

  1. Institute of Crop Sciences, Chinese Academy of Agricultural Sciences / National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing 100081, China
  • Received:2009-06-29 Revised:2009-09-09 Online:2010-02-10 Published:2009-12-21
  • Contact: shiyunsu@mail.caas.net.cn; Tel: 010-62186647

Abstract:

Dwarf germplasm is important for both breeding programs and basic researches in maize (Zea mays L.). Previously we found a naturally occurred dwarf mutant from the inbred “K36” and developed a new dwarf germplasm, “Ai 2003”, with good agronomical performance. Classical genetic analysis showed that this dwarfing character was controlled by a major single recessive nuclear gene. However, the character of plant height in the germplasm is also likely associated with other genetic loci. Therefore, quantitative trait locus (QTL) analysis was conducted to elucidate the genetic basis of plant height of this dwarf germplasm by using a segregating population consisting of 255 F2:3 lines derived from a cross between Ai 2003 and a normal inbred, Ji 257. A genetic linkage map was constructed by 114 polymorphism simple sequence repeat (SSR) markers covering the entire maize genome. The total map length was 2 852.1 cM, with an average distance of 27.4 cM between markers. Composite interval mapping (CIM) was used to identify the genes/QTLs controlled plant height based on the phenotypic characterization of 255 F2:3 families in Beijing and Hainan in 2006. The same three QTLs located on chromosomes 1 and 2 were identified under both environments, explaining 4.8% to 50.5% of the phenotypic variances, among which, one major QTL located in the region of bin 1.10–1.11 explained 50.5% and 37.5% of the phenotypic variation in Beijing and Hainan, respectively. A further sequences analysis revealed that the QTL is located in the 20–30 cM interval downstream of dwarf plant8 (d8), a well-known maize dwarf gene, implying that the locus is a gene newly discovered for controlling plant height in maize.

Key words: Maize, Dwarf germplasm, SSR, Plant height, QTL


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