Soybean,PI471938,Wilting resistance,Root traits,Genetic analysis,SSR markers,QTL,"/> Identification, Inheritance and QTL Mapping of Root and Shoot Traits in Soybean Variety PI471938 with Tolerance to Wilting
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Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (09): 1476-1483.doi: 10.3724/SP.J.1006.2010.01476


Identification, Inheritance and QTL Mapping of Root and Shoot Traits in Soybean Variety PI471938 with Tolerance to Wilting

LV Cai-Xia1,Guo-Jian-Qiu12**,WANG Ying1,LENG Jian-Tian1,YANG Guang-Ming1,HOU Wen-Sheng1,WU Cun-Xiang1*,HAN Tian-Fu1*   

  1. 1 National Key Facility for Crop Gene Resources and Genetic Improvement / Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 2. Luoyang Academy of Agricultural Sciences, Luoyang 471022, China
  • Received:2010-03-11 Revised:2010-04-23 Online:2010-09-12 Published:2010-07-05
  • Contact: HAN Tian-Fu,WU Cun-Xiang,,;Tel:010-82108784

Abstract: PI471938, introduced from the US, is a soybean [Glycine max (L.) Merr.] variety with tolerance to wilting. In order to know and use this germplasm, the shoot and root traits of PI471938 and two other varieties (Dare and Fengshouhuang) were investigated under either routine irrigation or drought stress conditions in this study. The crosses were made and segregating populations were analyzed for the inheritance of root and shoot traits by using the major gene and polygene mixed inheritance models. The results showed that the dry root weight, root volume and tap root length of PI471938 were significantly higher than those of Dare and Fengshouhuang under both routine irrigation and drought stress conditions, indicating that large root system especially the deep rooting is one of the major reasons for PI471938 to be tolerant to wilting. Both plant height and shoot dry weight of the parents and progenies were positively correlated with root dry weight and volume, which made it possible to use these shoot traits to indirectly select the root traits. In the F2 population of the cross Dare×PI416937, root dry weight was found to be mainly controlled by minor-effect polygenes under both routine irrigation and drought stress conditions. The root volume was controlled by polygenes under routine irrigation but by two pairs of additive-dominance-epistasis major genes plus additive-dominance polygenes under drought stress, and the heritability of major genes was as high as 54.63%. Under drought stress, the root dry weight and root volume in the segregating populations of the cross Fengshouhuang×PI471938 were mainly controlled by polygenes. By using the F2 population derived from Dare × PI471938 grown under drought condition, SSR mapping was conducted and five major-effect QTLs conferring the plant height, root dry weight, shoot/root ratio and tap root length were located on three linkage groups, which could explain 16.07%–38.44% of the total phenotypic variation.

Key words: Soybean')">

Soybean, PI471938, Wilting resistance, Root traits, Genetic analysis, SSR markers, QTL

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