Soybean,PI471938,Wilting resistance,Root traits,Genetic analysis,SSR markers,QTL,"/> Identification, Inheritance and QTL Mapping of Root and Shoot Traits in Soybean Variety PI471938 with Tolerance to Wilting
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Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (09): 1476-1483.doi: 10.3724/SP.J.1006.2010.01476

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Identification, Inheritance and QTL Mapping of Root and Shoot Traits in Soybean Variety PI471938 with Tolerance to Wilting

LV Cai-Xia1,Guo-Jian-Qiu12**,WANG Ying1,LENG Jian-Tian1,YANG Guang-Ming1,HOU Wen-Sheng1,WU Cun-Xiang1*,HAN Tian-Fu1*   

  1. 1 National Key Facility for Crop Gene Resources and Genetic Improvement / Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 2. Luoyang Academy of Agricultural Sciences, Luoyang 471022, China
  • Received:2010-03-11 Revised:2010-04-23 Online:2010-09-12 Published:2010-07-05
  • Contact: HAN Tian-Fu,WU Cun-Xiang,E-mail:hantf@mail.caas.net.cn,wucx@mail.caas.net.cn;Tel:010-82108784

Abstract: PI471938, introduced from the US, is a soybean [Glycine max (L.) Merr.] variety with tolerance to wilting. In order to know and use this germplasm, the shoot and root traits of PI471938 and two other varieties (Dare and Fengshouhuang) were investigated under either routine irrigation or drought stress conditions in this study. The crosses were made and segregating populations were analyzed for the inheritance of root and shoot traits by using the major gene and polygene mixed inheritance models. The results showed that the dry root weight, root volume and tap root length of PI471938 were significantly higher than those of Dare and Fengshouhuang under both routine irrigation and drought stress conditions, indicating that large root system especially the deep rooting is one of the major reasons for PI471938 to be tolerant to wilting. Both plant height and shoot dry weight of the parents and progenies were positively correlated with root dry weight and volume, which made it possible to use these shoot traits to indirectly select the root traits. In the F2 population of the cross Dare×PI416937, root dry weight was found to be mainly controlled by minor-effect polygenes under both routine irrigation and drought stress conditions. The root volume was controlled by polygenes under routine irrigation but by two pairs of additive-dominance-epistasis major genes plus additive-dominance polygenes under drought stress, and the heritability of major genes was as high as 54.63%. Under drought stress, the root dry weight and root volume in the segregating populations of the cross Fengshouhuang×PI471938 were mainly controlled by polygenes. By using the F2 population derived from Dare × PI471938 grown under drought condition, SSR mapping was conducted and five major-effect QTLs conferring the plant height, root dry weight, shoot/root ratio and tap root length were located on three linkage groups, which could explain 16.07%–38.44% of the total phenotypic variation.

Key words: Soybean')">

Soybean, PI471938, Wilting resistance, Root traits, Genetic analysis, SSR markers, QTL

[1] Liu X-Y(刘学义). Drought. In: Lam H-M(林汉明), Chang R-Z(常汝镇), Shao G-H(邵桂花), Liu Z-T(刘忠堂), eds. Soybean Stress Tolerance Research in China (中国大豆耐逆研究). Beijing: China Agriculture Press, 2009. pp 1-60 (in Chinese)
[2] Zhang S-G(张士功), Liu G-D(刘国栋), Liu G-L(刘更另). Plant nutrition and drought resistance of crops. Chin Bull Bot (植物学通报), 2001, 18(1): 64-69 (in Chinese with English abstract)
[3] Shan L(山仑), Chen G-L(陈国良). Theory and Practice of Dry Land Agriculture in the Loess Plateau (黄土高原旱地农业的理论与实践). Beijing: Science Press, 1993. pp 125-129 (in Chinese)
[4] Hudak C M, Patterson R P. Vegetative growth analysis of a drought-resistant soybean plant introduction
[J].Crop Sci.1995, 35:464-471
[5] Hudak C M, Patterson R P. Root distribution and soil moisture depletion pattern of a drought-resistant soybean plant introduction
[J].Agron J.1996, 88:478-485
[6] Pantalone V R, Rebetzke G J, Burton J W, Carter T E Jr. Phenotypic evaluation of root traits in soybean and applicability to plant breeding
[J].Crop Sci.1996, 36:456-459
[7] Carter T E Jr, De Souza P I, Purcell L C. Recent advances in breeding for drought and aluminum resistance in soybean. In: Kauffman H ed. Proceedings of World Soybean Research Conference VI, Chicago, IL, USA, 1999. pp 106-125
[8] Fletcher A L, Sinclair T R, Allen L H Jr. Transpiration responses to vapor pressure deficit in well watered “slow-wilting” and commercial soybean
[J].Environ Exp Bot.2007, 61:145-151
[9] Hufstetler E V, Boerma H R, Carter T E Jr, Earl H J. Genotypic variation for three physiological traits affecting drought tolerance in soybean
[J].Crop Sci.2007, 47:25-35
[10] King C A, Purcell L C, Brye K R. Differential wilting among soybean genotypes in response to water deficit
[J].Crop Sci.2009, 49:290-291
[11] Sinclair T R, Zwieniecki M A, Holbrook N M. Low leaf hydraulic conductance associated with drought tolerance in soybean
[J].Physiol Plant.2008, 132:446-451
[12] Carter T E Jr, Rufty T W. Soybean plant introductions exhibiting drought and aluminum tolerance. In: Kuo G ed. Adaptation of Vegetable and Other Food Crops to Temperature and Water Stress. Asian Vegetable Research and Development Center, Shanhua, Taiwan, China, 1993. pp 335-346
[13] Sloane R J, Patterson R P, Carter T E Jr. Field drought tolerance of a soybean plant introduction
[J].Crop Sci.1990, 30:118-123
[14] Pantalone V R, Buton J W, Carter T E Jr. Soybean fibrous root heritability and genotypic correlations with agronomic and seed quality traits
[J].Crop Sci.1996, 36:1120-1125
[15] Fehr W R, Caviness C E. Stages of Soybean Development. Agric and Home Economics Exp. 1977, Stn. Spec. Rep. 80, Iowa State Univ, Ames, IA, USA, pp 1-11
[16] Lü S-L(吕世霖), Cheng S-H(程舜华). The ecological distribution of seed characteristics of soybean and in relation to breeding. Soybean Sci (大豆科学), 1984, 3(3): 201-207 (in Chinese with English abstract)
[17] Xue Q-W(薛青武), Chen P-Y(陈培元). Effect of different types of drought stress on water relations and photosynthesis in wheat. Acta Agric Boreali-Sin (华北农学报), 1990, 5(2): 26-32 (in Chinese with English abstract)
[18] Wang J-K(王建康), Gai J-Y(盖钧镒). Identification of major gene and polygene mixed inheritance model of quantitative traits by using joint analysis of P1, F1, P2, F2 and F2:3 generations. Acta Agron Sin (作物学报), 1998, 24(6): 651-659 (in Chinese with English abstract)
[19] Gai J-Y(盖钧镒), Zhang Y-M(章元明), Wang J-K(王建康). Genetic System of Quantitative Traits in Plants (植物数量性状遗传体系). Beijing: Science Press, 2003. pp 169-219 (in Chinese)
[20] Lincoin S E, Daly M J, Lander E S. Constructing genetics linkage maps with MAPMAKER/EXP Version 3.0: A Tutorial and Reference Manual. Cambridge: Lander E S, 1993. pp 1-9
[21] Wang S C, Basten C J, Zeng Z B. Windows QTL Cartographer 2.5 User Manual. Department of Statistics, North Carolina State University, Raleigh, NC, USA, 2005. http://statgen.ncsu.edu/ qtlcart/WQTLCart.htm
[22] Kabelka E A, Diers B W, Fehr W F, LeRoy A R, Baianu I C, You T, Neece D J, Nelson R L. Putative alleles for increased yield from soybean plant introductions
[J].Crop Sci.2004, 44:784-791
[23] Pantalone V R, Buton J W, Carter T E Jr. Soybean fibrous root heritability and genotypic correlations with agronomic and seed quality traits
[J].Crop Sci.1996, 36:1120-1125
[24] Wang H-L(王宏林), Yu D-Y(喻德跃), Wang Y-J(王永军), Chen S-Y(陈受宜), Gai J-Y(盖钧镒). Mapping QTLs of soybean root weight with RIL population NJRIKY. Hereditas (遗传), 2004, 26 (3): 333-336 (in Chinese with English abstract)
[25] Liu Y(刘莹), Gai J-Y(盖钧镒), Lü H-N(吕慧能), Wang Y-J(王永军), Chen S-Y(陈受宜). Identification of drought tolerant germplasm and inheritance and QTL mapping of related root traits in soybean
[Glycine max (L.) Merr.]. Act Genet Sin (遗传学报), 2005, 32(8): 855-863 (in Chinese with English abstract)
[26] Yang S-P(杨守萍), Chen J-M(陈加敏), He X-H(何小红), Yu D-Y(喻德跃), Gai J-Y(盖钧镒). Inheritance of drought tolerance and root traits of seedling in soybean. Soybean Sci (大豆科学), 2005, 24(4): 275-280 (in Chinese with English abstract)
[27] Du W, Yu D, Fu S. Detection of quantitative trait loci for yield and drought tolerance traits in soybean using a recombinant inbred line population
[J].J Integr Plant Biol.2009, 51:868-878
[28] Manavalan L P, Guttikonda S K, Tran L S P, Nguyen H T. Physiological and molecular approaches to improve drought resistance in soybean
[J].Plant Cell Physiol.2009, 50:1260-1276
[29] Guan J-F(关军锋), Li G-M(李广敏). The root function expression and regulation under drought stress. China Basic Sci (中国基础科学), 2001, (3): 25-28 (in Chinese with English abstract)
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