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Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (12): 2055-2061.doi: 10.3724/SP.J.1006.2010.02055

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Application of SCoT Molecular Marker Technique in Genus Arachis

XIONG Fa-Qian1,2,3,JIANG Jing1,ZHONG Rui-Chun1,HAN Zhu-Qiang1,HE Liang-Qiong1,LI Zhong1,ZHUANG Wei-Jian4,*,TANG Rong-Hua1,2, *   

  1. 1 Cash Crops Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China; 2 Guangxi Crop Genetic Improvement and Biotechnology Laboratory, Guangxi Academy of Agricultural Sciences, Nanning 530007, China; 3 College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China; 4 College of Crop Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China
  • Received:2010-04-09 Revised:2010-08-05 Online:2010-12-12 Published:2010-10-09
  • Contact: TANG Rong-Hua,E-mail:tronghua@163.com,Tel: 0771-3276055;ZHUANG Wei-Jian,E-mail:zhuangwj@pub2.fz.fj.cn

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Abstract: The study of molecular markers in the genus Arachis is far behind other species. It was difficult to identify DNA polymorphism in cultivated peanut by most of the molecular marker techniques, because genetic base in cultivated peanut is narrow, limiting the utilization of the Arachis wild species to improve the cultivated peanut and the establishment of peanut breeding technologies of MAS. In the present study, SCoT molecular marker technique, which can be used to detect markers associated with functional genes, was firstly used to study the genetic diversity and relationships among sixteen accessions of four sections in the genus Arachis and among eight accessions of A. hypogaea, respectively. The results showed that a total of 194 loci were detected with 23 SCoT primers in the tested Arachis accessions, 130 of which were polymorphic with a polymorphism of 67.01%. The genetic relationships revealed by cluster analysis were in accordance with previous reports. Nineteen polymorphic SCoT primers were screened out, and a total of 198 loci were amplified in the tested cultivated peanut accessions, 67 (33.84%) of which were polymorphic. This indicated that SCoT marker technique can be used to detect a certain amount of DNA polymorphism in cultivated peanut.

Key words: Peanut, Start codon targeted polymorphism (SCoT), Molecular marker, Genetic diversity

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