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Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (01): 112-118.doi: 10.3724/SP.J.1006.2011.00112


Alleviation of Drought Stress Inhibition on Photosynthesis by Over Expression of PEPC Gene in Rice

ZHOU Bao-Yuan,DING Zai-Song,ZHAO Ming*   

  1. Institute of Crop Sciences, Chinese Academy of Agricultural Sciences / Key Laboratory of Crop Ecophysiology and Cultivition, Ministry of Agriculture, Beijing 100081, China
  • Received:2010-04-01 Revised:2010-04-01 Online:2011-01-12 Published:2010-11-16

Abstract: Introducingenzymes involved in photosynthesis of C4 plants into rice is supposed to enhance the photosynthesis and crop productivity. However, only a few researches showed that the photosynthesis and crop productivity has been improved by introducing phosphoenolpyruvate carboxylase (PEPC) gene into rice. In the present research, the photosynthesis in 42 lines of PEPC gene overexpressed rice was investigated. The average photosynthetic rate (Pn) of transgenic rice lines was almost the same as that of the wild type (control) in paddy field, while much higher in upland field. Only a few transgenic lines showed higher Pn in paddy field and most of them showed higher Pn in upland field. Similar results were found in the water controlled experiment. Two transgenic rice lines with different relative activities of PEPC (10 and 25 fold) were selected to study their photosynthesis under different water potentials (0, -20, and -40 kPa). In both lines, Pn was similar with that in the wild type under normal condition (0 kPa) and much higher under drought conditions (-20 and -40 kPa). In both experiments, the transgenic lines had higher Pn under drought conditions, with a much slower decreasing rate than the wild type. Therefore, the present results suggested that the overexpressed PEPC could not improve the photosynthetic rate of transgenic rice plants. But the photosynthetic rate of transgenic rices declined slowly under drought condition. So it is supposed that PEPC might be involved in drought resistance to decrease the inhibition of drought stress on photosynthesis in rice.

Key words: Drought, Transgenic rice, Phosphoenolpyruvate carboxylase (PEPC), Photosynthetic rate

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