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Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (01): 105-111.doi: 10.3724/SP.J.1006.2011.00105

• TILLAGE & CULTIVATION · PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Effects of Different Fertilizers on Functional Diversities of Microbial Flora in Rhizospheric Soil of Monoculture Tobacco

YANG Yu-Hong1,CHEN Dong-Mei2,JI Yan1,WANG Hai-Bin2,DUAN Yu-Qi1,GUO Xu-Kui2,HE Hai-Bin2,LIN Wen-Xiong2,*   

  1. 1 Institute of Tobacco Research, Yuxi 653100, China; 2Agroecological institute, Fujian Agriculture and Forestry University, Fuzhou 350002, China
  • Received:2010-06-16 Revised:2010-09-26 Online:2011-01-12 Published:2010-11-16

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Abstract: Tobacco is an intolerant continuous cropping crop. Continuous cropping obstacles restrict sustainable development of tobacco production, which are the most important issues in agricultural production that need to be solved urgently. In this study, tobacco was consecutively planted for twelve years in the same soil and the rhizospheric soil was sampled to investigate the changes in the functional diversities of microbial flora after different fertilizer treatments. The results showed that the autotoxic allelopathic potential was maximum for the monoculture soil treated with traditional compound fertilizer, and minimum for the soil treated with farmyard manure. BIOLOG analysis found that the traditional compound fertilizer was conducive to the growth of the microbial flora feeding on amino acids and amine as carbon sources. The commercial organic fertilizer was favorable to the growth of the microbial flora using carboxylic acids as a carbon source, and farmyard manure was good for the growth of the microbial flora feeding on carbohydrate, fatty acids and phenolic acids as carbon sources. Principal component analysis indicated that the first two components related to carbon sources accounted for 74.37% and 25.63% of the data variation, respectively. The carbon source of carbohydrate, fatty acids and phenolic acids made a mainly contribution to the separation of two principal components. Correlation analysis indicated that the autotoxic allelopathic potential of tobacco rhizospheric soil was positively correlated with the AWCD value of microbial flora feeding on carbohydrate and phenolic acids as carbon sources, and negatively correlated with that of the microbial flora using the carbon source of fatty acids. In addition, for the growth of microbial flora in monoculture soil, farmyard manure was the best, commercial organic fertilizer showed the medium effect, and traditional compound fertilizer was the worst.

Key words: Tobacco, Consecutive cropping problem, Microbial flora, Functional diversity, BIOLOG

[1]Jin Y(晋艳), Yang Y-H(杨宇虹), Duan Y-Q(段玉琪), Kong G-H(孔光辉). Effect of rotational cropping and continuous cropping on yield and quality of flue-cured tobacco. Southwest China J Agric Sci (西南农业学报), 2004, 17(S1): 267–271 (in Chinese with English abstract)
[2]Freddy A. Common bean response to tillage intensity and weed control strategies. Cropping Syst, 2001, 93: 556–563
[3]Yu J Q. Autotoxic potential in vegetables crops. In: Narwal S S ed. Ailelopathy Update-Basic and Applied Aspects, 1999. pp 149–162
[4]Zhang X-L(张晓玲), Pan Z-G(潘振刚), Zhou X-F(周晓锋), Ni W-Z(倪吾钟). Continuous cropping obstacle and autotoxicity. Chin J Soil Sci (土壤通报), 2007, 38(4): 267–271(in Chinese with English abstract)
[5]Wu F-Z(吴凤芝), Zhao F-Y(赵风艳), Gu S-Y(谷思玉). Effect of the continuous cultivating cucumber on the bio-chemical properties of soil in the plastic greenhouse. System Sci Comprehensive Studies Agric (农业系统科学与综合研究), 2002, 18(1): 20–22 (in Chinese with English abstract)
[6]Pan M-Y(盘莫谊), Zhang Y-Z(张杨珠), Xiao N-Q(肖嫩群), Tan Z-J(谭周进). Effect of continuous cropping tobacco on the microbes and enzyme activities in dry land soil. World Sci-Tech R&D (世界科技研究与发展), 2008, 30(3): 295–297 (in Chinese with English abstract)
[7]Chen D-M(陈冬梅), Ke W-H(柯文辉), Chen L-L(陈兰兰), Huang J-W(黄锦文), Wu W-X(吴文祥), Chen T(陈婷), Zhang Z-Y(张重义), Lin W-X(林文雄). Diversity of bacterial community in rhizosphere soils under effects of continuously planting burley tobacco. Chin J Appl Ecol (应用生态学报), 2010, 21(7): 1751–1758 (in Chinese with English abstract)
[8]Xue X-P(薛小平), Zhang Q(张琴), Chen Y(陈懿), Wang M-S(王茂胜), Pan W-J(潘文杰), Li J-X(李继新). Effects of fertilization and microorganism on growth of continuous cropping tobacco seedling. Acta Agric Jiangxi (江西农业学报), 2008, 20(9): 1–4 (in Chinese with English abstract)
[9]Wang C(王超), Wu F(吴凡), Liu X-L(刘训理), Liu B(刘兵). Tobacco rhizosphere microorganism in different fertility of soil. Chin Tob Sci (中国烟草科学), 2005, (2): 12–14 (in Chinese with English abstract)
[10]Guo H-H(郭汉华), Yi J-H(易建华), Jia Z-H(贾志红), Sun Z-J(孙在军), Li H-W(李挥文). Effect of fertilizaion on tobacco growth and microbe quantity in rhizosphere soil. Tob Sci Technol (烟草科技), 2004, (6): 40–42 (in Chinese with English abstract)
[11]Wu J-F(吴建峰), Lin X-G(林业贵). Effect of soil microbes on plant growth. Soils (土壤), 2001, (1): 18–21 (in Chinese with English abstract)
[12]Guo H-X(郭红祥), Liu W-Q(刘卫群), Jiang Z-S(姜占省). Effect of cake seed sesame fertilizer on soil microbe in root system of flue-cured tobacco. J Henan Agric Univ (河南农业大学学报), 2002, (4): 344–347 (in Chinese with English abstract)
[13]Xia Z-Y(夏振远), Li Y-H(李云华), Yang S-J(杨数军). Effects of microbial fertilizer on flue-cured tobacco on field. Chin Tob Sci (中国烟草科学), 2002, (3): 28–30 (in Chinese with English abstract)
[14]Lin R-Y(林瑞余), Rong H(戎红), Zhou J-J(周军建), Yu C-P(于翠平), Ye C-Y(叶陈英), Chen L-S(陈良生), Lin W-X(林文雄). Impact of rice seedling allelopathy on rhizospheric microbial populations and their functional diversities. Acta Ecol Sin (生态学报), 2007, 27(9): 3644–3654 (in Chinese with English abstract)
[15]Tan X-M(谭秀梅), Wang H-T(王华田), Kong L-G(孔令刚), Wang Y-P(王延平). Accumulation of phenolic acids in soil of a continuous cropping Poplar plantation and their effects on soil microbes. J Shandong Univ (Nat Sci)(山东大学学报·理学版), 2008, 43(1): 14–19 (in Chinese with English abstract)
[16]Zhang S-X(张淑香), Gao Z-Q(高子勤), Liu H-L(刘海玲). Continuous cropping obstacle and rhizospheric microecology: III. Soil phenolic acids and their biological effect. Chin J Appl Ecol (应用生态学报), 2000, 11(5): 102–105 (in Chinese with English abstract)
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