Welcome to Acta Agronomica Sinica，

Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (01): 112-118.doi: 10.3724/SP.J.1006.2011.00112

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Alleviation of Drought Stress Inhibition on Photosynthesis by Over Expression of PEPC Gene in Rice

ZHOU Bao-Yuan,DING Zai-Song,ZHAO Ming*   

  1. Institute of Crop Sciences, Chinese Academy of Agricultural Sciences / Key Laboratory of Crop Ecophysiology and Cultivition, Ministry of Agriculture, Beijing 100081, China
  • Received:2010-04-01 Revised:2010-04-01 Online:2011-01-12 Published:2010-11-16

PDF

1842

Cited

26

Abstract: Introducingenzymes involved in photosynthesis of C4 plants into rice is supposed to enhance the photosynthesis and crop productivity. However, only a few researches showed that the photosynthesis and crop productivity has been improved by introducing phosphoenolpyruvate carboxylase (PEPC) gene into rice. In the present research, the photosynthesis in 42 lines of PEPC gene overexpressed rice was investigated. The average photosynthetic rate (Pn) of transgenic rice lines was almost the same as that of the wild type (control) in paddy field, while much higher in upland field. Only a few transgenic lines showed higher Pn in paddy field and most of them showed higher Pn in upland field. Similar results were found in the water controlled experiment. Two transgenic rice lines with different relative activities of PEPC (10 and 25 fold) were selected to study their photosynthesis under different water potentials (0, -20, and -40 kPa). In both lines, Pn was similar with that in the wild type under normal condition (0 kPa) and much higher under drought conditions (-20 and -40 kPa). In both experiments, the transgenic lines had higher Pn under drought conditions, with a much slower decreasing rate than the wild type. Therefore, the present results suggested that the overexpressed PEPC could not improve the photosynthetic rate of transgenic rice plants. But the photosynthetic rate of transgenic rices declined slowly under drought condition. So it is supposed that PEPC might be involved in drought resistance to decrease the inhibition of drought stress on photosynthesis in rice.

Key words: Drought, Transgenic rice, Phosphoenolpyruvate carboxylase (PEPC), Photosynthetic rate

[1]Häusler R E, Hirsch H J, Kreuzaler F, Peterhänsel C. Over-expression of C4-cycle enzymes in transgenic C3 plants: a biotechnological approach to improve C3-photosynthesis. J Exp Bot, 2002, 53: 591-607
[2]Lee good R. C4 photosynthesis: principles of CO2 concentration and prospects for its introduction into C3 plants. J Exp Bot, 2002, 53: 581-591
[3]Matsuoka M, Furbank R T, Fukayama H, Miyao M. Molecular engineering of C4 photosynthesis. Annu Rev Plant Physiol Plant Mol Biol, 2001, 52: 297-314
[4]Mann C C. Crop scientists seek a new revolution. Science, 1999, 283: 310-314
[5]Mann C C. Genetic engineers aim to soup up crop photosynthesis. Science, 1999, 283: 314-316
[6]Ku M S B, Agarie S, Nomurn M, Fukayama H, Tsuchida H, Ono K,Hirose S, Toki S, Miyao M, Matsuoka M. High-level expression of maize phosphoenolpyruvate carboxylase in transgenic rice plants. Nat Biotech, 1999, 17: 76-80
[7]Sheehy J E, Mitchell P L, Hardy B. Redesign rice Photosynthesis to Increase Yield. Amsterdam: Elsvier Science Publishers, 2000. pp 167-204
[8]Agarie S, Miura A, Sumikura R, Tsukamoto S, Nose A, Arima S, Matsuoka M, Miyao-Tokutomi M. Over expression of C4 PEPC caused O2-insensitive photosynthesis in transgenic rice plant. Plant Sci, 2002, 162: 257-265
[9]Fukayama H, Hatch M D, Tamai T, Tsuchida H, Sudoh S, Furbank R T, Miyao M. Activity regulation and physiological impacts of maize C4-specific phosphoenolpyruvate carboxylase overproduced in transgenic rice plants. Photosynth Res, 2003, 77: 227-239
[10]Ding Z-S(丁在松), Zhao M(赵明), Jing Y-X(荆玉祥), Li L-B(李良璧), Kuang T-Y(匡廷云). Effect of over expression of maize ppc gene on photosynthesis in transgenic rice plants. Acta Agron Sin (作物学报), 2007, 33(5): 717-722 (in Chinese with English abstract)
[11]Miyao M. Molecular evolution and genetic engineering of C4 photosynthetic enzymes. J Exp Bot, 2003, 54: 179-189
[12]Ku M S B, Cho D, Ranade U, Hsu T P, Li X, Jiao D M, Ehleringer J, Miyao M, Matsuoka M. Photosynthetic performance of transgenic rice plants over expressing maize C4 photosynthesis enzymes. In: Sheehy J E, Mitchell P L, Hardy B, eds. Redesigning of Rice Photosynthesis to Increase Yield. Amsterdam: Elsevier Science Publishers, 2000. pp 193-204
[13]Jiao D M, Huang X Q, Li X, Chi W, Kuang T Y, Zhang Q D, Ku M S B, Cho D H. Photosynthetic characteristics and tolerance to photo-oxidation of transgenic rice expressing C4 photosynthesis enzymes. Photosynth Res, 2002, 72: 85-93
[14]Jiao D M, Li X, Ji B H. Photoprotective effects of high level expression of C4 phosphoenolpyruvate carboxylase in transgenic rice during photo inhibition. Photosynthetica, 2005, 43: 501-508
[15]Jiao D-M(焦德茂), Li X(李霞), Huang X-Q(黄雪清), Chi W(迟伟), Kuang T-Y(匡廷云), Ku M S B(古森本). Characteristics of photosynthetic CO2 assimilation and chlorophyll fluorescence in transgenic rice plants with PEPC gene. Chin Sci Bull (科学通报), 2001, 46(5): 414-418 (in Chinese with English abstract)
[16]Bandyopadhyay, Datta K, Zhang J, Yang W, Raychaudhuri S, Datta S K. Enhanced photosynthesis rate in genetically engineered indica rice expressing pepc gene cloned from maize. Plant Sci, 2007, 172: 1204-1209
[17]Gonzalez M C, Sanchez R, Cejudo F J. Abiotic stresses affecting water balance induce phosphoenolpyruvate carboxylase expression in roots of wheat seedlings. Planta, 2003, 216: 985-992
[18]Sanchez R, Flores A, Cejudo F J. Arabidopsis phosphoenolpyruvate carboxylase genes encode immunologically unrelated polypeptides and are differentially expressed in response to drought and salt stress. Planta, 2006, 223: 901-909
[19]Echevarría C, Garciá-Maurino S, Alvarez R, Soler A, Vidal J. Salt stress increases the Ca2+-independent phosphoenolpyruvate carboxylase kinase activity in Sorghum leaves. Planta, 2001, 214: 283-287
[20]Garciá-Maurino S, Monreal J A, Alvarez R, Vidal J, Echevarría C. Characterization of salt stress-enhanced phosphoenolpyruvate carboxylase kinase activity in leaves of Sorghum vulgare: independence of osmotic stress, involvement of iontoxicity and significance of dark phosphorylation. Planta, 2003, 216: 648-655
[21]Barlow E W K. The Growth and Functioning of Leaves. London: Cambridge University Press, 1988. pp 314-345
[22]Legg B J, Day W, Lawtor D W, Parkinson K J. The effect of drought on barley growth: Models and measurements showing relative importance of leaf area and photosynthetic rate. J Agric Sci, 1979, 92: 703-7161
[23]Yamance K, Hayakawa K, Kawasaki M. Bundle sheath chloroplasts of rice are more sensitive to drought stress than mesophyll chloroplasts. J Plant Physiol, 2003, 160: 1319-1327
[24]Jiang M-Y(蒋明义), Yang W-Y(杨文英), Xu J(徐江), Chen Q-Y(陈巧云). Osmotic stress-induced oxidative injury of riceseedlings. Acta Agron Sin (作物学报), 1994, 20(6): 733-738 (in Chinese with English abstract)
[25]Dhindsa R S. Protein synthesis during rehydration of rapidly dried Tortula ruralis: evidence for oxidation injury. Plant Physiol, 1987, 85: 1094-1098
[26]Kavi Kishore P B, Sangam S, Amrutha R N, Laxmi P S, Naidu K R, Rao K R S S, Rao S, Reddy K J, Theriappan P, Sreenivasulu N. Regulation of proline biosynthesis, degradation, uptake and transport in higher plants: Its implications in plant growth and abiotic stress tolerance. Curr Sci, 2005, 88: 424-438
[27]Liang J-S(梁建生), Zhang J-H(张建华). Production, transport and physiological functions of stress signal abscisic acid in roots. Plant Physiol Commun (植物生理学通讯), 1998, 34(5): 329-338 (in Chinese with English abstract)
[28]Grabov A, Blatt M R. Co-ordination of signaling elements in guard cell ion channel control. J Exp Bot, 1998, 49: 351-360
[29]Fang L-F(方立锋), Ding Z-S(丁在松), Zhao M(赵明). Characteristics of drought tolerance in ppc overexpressed rice seedlings. Acta Agron Sin (作物学报), 2008, 34(7): 1220-1226 (in Chinese with English abstract)
[30]Kung S D, Chollet R, Marsho T V. Crystallization and assay procedures of tobacco ribulose 1,5-bisphosphate carboxylase oxygenase. In: Pietro A S ed. Method Enzymology. New York: Academic Press Inc, 1980, 69: 326-335
[31]Gonzalez D H, Iglesias A A, Andreo C S. On the regulation of phosphoenolpyruvate carboxylase activity from maize leaves by L-malate: effect of pH. J Plant Physiol, 1984, 116: 425-430
[32]Frauke C, Peter S, Jurgen F. Malate metabolism and reactions of oxidoreduction in cold-hardened winter rye (Secale cereale L.) leaves. J Exp Bot, 2003, 384: 1075-1083
[33]Backhausen J E, Kitzmann C, Scheibe R. Competition between electron acceptors in photosynthesis: regulation of the malate valve during CO2 fixation and nitrite reduction. Photosynth Res, 1994, 42: 75-86
[34]Kogami H, Shono M, Koike T, Yanagisawa S, Izui K, Sentoku N, Tanifuji S, Uchimiya H, Toki S. Molecular and physiological evaluation of transgenic tobacco plants expression a maize phosphoenolpyruvate carboxylase gene under the control of the cauliflower mosaic virus 35S promoter. Transgenic Res, 1994, 3: 287-296
[35]Li X(李霞), Jiao D-M(焦德茂), Dai C-C(戴传超). The response to photo oxidation in leaves of PEPC transgenic rice plant (Oryza sativa L.). Acta Agron Sin (作物学报), 2005, 31(4): 408-413 (in Chinese with English abstract)
[36]Andreo C S, Gonzalez D H, Iglesias A A. Higher plant phosphoenolpyruvate carboxylase: Structure and regulation. FEBS Lett, 1987, 13: 1-8
[37]Jiao D-M(焦德茂), Kuang T-Y(匡廷云), Li X(李霞), Ge Q-Y(戈巧英), Huang X-Q(黄雪清), Hao N-B(郝乃斌), Bai K-Z(白克智). A limited photosynthetic CO2 concentration mechanism in transgenic rice plant over expressed maize PEPC gene. Sci China (Ser C) (中国科学·C辑), 2003, 33(1): 33-39 (in Chinese with English abstract)
[1] CHEN Song-Yu, DING Yi-Juan, SUN Jun-Ming, HUANG Deng-Wen, YANG Nan, DAI Yu-Han, WAN Hua-Fang, QIAN Wei. Genome-wide identification of BnCNGC and the gene expression analysis in Brassica napus challenged with Sclerotinia sclerotiorum and PEG-simulated drought [J]. Acta Agronomica Sinica, 2022, 48(6): 1357-1371.
[2] ZHOU Wen-Qi, QIANG Xiao-Xia, WANG Sen, JIANG Jing-Wen, WEI Wan-Rong. Mechanism of drought and salt tolerance of OsLPL2/PIR gene in rice [J]. Acta Agronomica Sinica, 2022, 48(6): 1401-1415.
[3] YAN Jia-Qian, GU Yi-Biao, XUE Zhang-Yi, ZHOU Tian-Yang, GE Qian-Qian, ZHANG Hao, LIU Li-Jun, WANG Zhi-Qin, GU Jun-Fei, YANG Jian-Chang, ZHOU Zhen-Ling, XU Da-Yong. Different responses of rice cultivars to salt stress and the underlying mechanisms [J]. Acta Agronomica Sinica, 2022, 48(6): 1463-1475.
[4] LI Yi-Jun, LYU Hou-Quan. Effect of agricultural meteorological disasters on the production corn in the Northeast China [J]. Acta Agronomica Sinica, 2022, 48(6): 1537-1545.
[5] WANG Xing-Rong, LI Yue, ZHANG Yan-Jun, LI Yong-Sheng, WANG Jun-Cheng, XU Yin-Ping, QI Xu-Sheng. Drought resistance identification and drought resistance indexes screening of Tibetan hulless barley resources at adult stage [J]. Acta Agronomica Sinica, 2022, 48(5): 1279-1287.
[6] LI A-Li, FENG Ya-Nan, LI Ping, ZHANG Dong-Sheng, ZONG Yu-Zheng, LIN Wen, HAO Xing-Yu. Transcriptome analysis of leaves responses to elevated CO2 concentration, drought and interaction conditions in soybean [Glycine max (Linn.) Merr.] [J]. Acta Agronomica Sinica, 2022, 48(5): 1103-1118.
[7] WANG Xia, YIN Xiao-Yu, Yu Xiao-Ming, LIU Xiao-Dan. Effects of drought hardening on contemporary expression of drought stress memory genes and DNA methylation in promoter of B73 inbred progeny [J]. Acta Agronomica Sinica, 2022, 48(5): 1191-1198.
[8] DING Hong, XU Yang, ZHANG Guan-Chu, QIN Fei-Fei, DAI Liang-Xiang, ZHANG Zhi-Meng. Effects of drought at different growth stages and nitrogen application on nitrogen absorption and utilization in peanut [J]. Acta Agronomica Sinica, 2022, 48(3): 695-703.
[9] ZHANG Hai-Yan, XIE Bei-Tao, JIANG Chang-Song, FENG Xiang-Yang, ZHANG Qiao, DONG Shun-Xu, WANG Bao-Qing, ZHANG Li-Ming, QIN Zhen, DUAN Wen-Xue. Screening of leaf physiological characteristics and drought-tolerant indexes of sweetpotato cultivars with drought resistance [J]. Acta Agronomica Sinica, 2022, 48(2): 518-528.
[10] CAO Liang, DU Xin, YU Gao-Bo, JIN Xi-Jun, ZHANG Ming-Cong, REN Chun-Yuan, WANG Meng-Xue, ZHANG Yu-Xian. Regulation of carbon and nitrogen metabolism in leaf of soybean cultivar Suinong 26 at seed-filling stage under drought stress by exogenous melatonin [J]. Acta Agronomica Sinica, 2021, 47(9): 1779-1790.
[11] ZHANG Ming-Cong, HE Song-Yu, QIN Bin, WANG Meng-Xue, JIN Xi-Jun, REN Chun-Yuan, WU Yao-Kun, ZHANG Yu-Xian. Effects of exogenous melatonin on morphology, photosynthetic physiology, and yield of spring soybean variety Suinong 26 under drought stress [J]. Acta Agronomica Sinica, 2021, 47(9): 1791-1805.
[12] YUE Dan-Dan, HAN Bei, Abid Ullah, ZHANG Xian-Long, YANG Xi-Yan. Fungi diversity analysis of rhizosphere under drought conditions in cotton [J]. Acta Agronomica Sinica, 2021, 47(9): 1806-1815.
[13] LI Jie, FU Hui, YAO Xiao-Hua, WU Kun-Lun. Differentially expressed protein analysis of different drought tolerance hulless barley leaves [J]. Acta Agronomica Sinica, 2021, 47(7): 1248-1258.
[14] LI Hui, LI De-Fang, DENG Yong, PAN Gen, CHEN An-Guo, ZHAO Li-Ning, TANG Hui-Juan. Expression analysis of abiotic stress response gene HcWRKY71 in kenaf and transformation of Arabidopsis [J]. Acta Agronomica Sinica, 2021, 47(6): 1090-1099.
[15] LI Peng-Cheng, BI Zhen-Zhen, SUN Chao, QIN Tian-Yuan, LIANG Wen-Jun, WANG Yi-Hao, XU De-Rong, LIU Yu-Hui, ZHANG Jun-Lian, BAI Jiang-Ping. Key genes mining of DNA methylation involved in regulating drought stress response in potato [J]. Acta Agronomica Sinica, 2021, 47(4): 599-612.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No. 80 Xueyuan South Rd, Beijing 100081, P. R. China E-mail: zwxb301@caas.cn
Supported by Beijing Magtech Co.Ltd