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Acta Agronomica Sinica ›› 2025, Vol. 51 ›› Issue (2): 347-357.doi: 10.3724/SP.J.1006.2025.42030

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Map-based cloning and functional analysis of Dwarf and Tillering 1 (DT1) gene in rice

LI Chun-Mei,CHEN Jie,LANG Xing-Xuan,ZHUANG Hai-Min,ZHU Jing,DU Zi-Jun,FENG Hao-Tian,JIN Han,ZHU Guo-Lin,LIU Kai*   

  1. Zhongkai University of Agriculture and Engineering, Guangzhou 510225, Guangdong, China
  • Received:2024-06-25 Revised:2024-09-18 Accepted:2024-09-18 Online:2025-02-12 Published:2024-10-10
  • Supported by:
    This study was supported by the State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China (SKL-KF202315), the State Key Laboratory for Managing Biotic and Chemical Treats to the Quality and Safety of Agro-products (2021DG700024-KF202408), the Basic and Applied Basic Research Foundation of Guangdong Province (2024A1515013028), the Science and Technology Plan Project of Yunfu (2023020202), the Guangdong Provincial Universities Characteristic Innovation Project (2023KTSCX046), and the Guangzhou Science and Technology Plan Project (2024A04J4995).

Abstract:

Tillering is a crucial trait that influences plant architecture and yield in rice. In this study, we identified a natural mutant with dwarf stature and high tillering, which we designated as dwarf and tillering 1 (dt1). The dt1 mutant exhibited significant reductions in panicle length, seed setting rate, grain length, grain width, thousand-grain weight, and the number and size of vascular bundle sheath cells compared to the wild type. Map-based cloning revealed that the dt1 phenotype was caused by an 8 bp insertion in the second exon of D17/HTD1 (LOC_Os04g46470), which encodes Carotenoid Cleavage Dioxygenase 7 (CCD7), a key enzyme in strigolactone biosynthesis. Thus, dt1 represents a new allele of D17/HTD1. Additionally, the dt1 mutant showed significantly reduced germination rate, root length, and root diameter, all of which were restored by the exogenous application of the strigolactone analog GR24. Transcriptomic analysis identified 579 up-regulated and 506 down-regulated genes in the dt1 mutant. Gene Ontology (GO) analysis revealed that the up-regulated genes were significantly enriched in pathways related to auxin response, endogenous stimulus response, and hormone response, while the down-regulated genes were enriched in pathways involved in cellular carbohydrate metabolism and histone methylation. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis indicated that the up-regulated genes were associated with plant hormone signal transduction, whereas the down-regulated genes were linked to amino sugar and nucleotide sugar metabolism, as well as diterpenoid biosynthesis. These findings enhance our understanding of the regulatory roles of CCD7 and strigolactones in rice and hold significant theoretical implications for rice breeding.

Key words: rice, dwarf and tillering, map-based cloning, biological function

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