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Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (04): 711-716.doi: 10.3724/SP.J.1006.2011.00711


Physiological Basis of Improving Soluble Sugar Content in Sweetpotato for Table Use by Humic Acid Application

LIU Hong-Juan1,LI Zuo-Mei1,SHI Chun-Yu1,*,ZHANG Li-Ming2,*   

  1. 1 Agronomy College, Shandong Agricultural University / State Key Laboratory of Crop Biology, Tai’an 271018, China; 2 Shandong Academy of Agricultural Sciences, Jinan 250100, China
  • Received:2010-09-27 Revised:2011-01-06 Online:2011-04-12 Published:2011-02-24
  • Contact: 史春余, E-mail: scyu@sdau.edu.cn, Tel: 0538-8246259; 张立明, E-mail: zhanglm11@sina.com.cn

Abstract: Soluble sugar content is a main index of edible quality in storage root of sweetpotato for table use.  Previous research showed that soluble sugar content in storage organs of plants was increased significantly by application of humic acid (HA), butthere have been little research on their physiological basis. The objective of this study was to clarify the physiological basis of soluble sugar content improvement in storage root of sweetpotato by using humic acid. The typical varieties of sweetpotato for table use (Beijing 553) was grown in a replicated experiment at agricultural experiment station of Shandong Agricultural University in two summer growing seasons of 2008 and 2009. The treatments included the control and treatment applied HA. The changes of starch content, soluble sugar content and relative enzymes activity during root tuber development, and sucrose content and relative enzymes activity in functional leaves were determined by using periodic sampling during storage root development. The results showed that compared with the control, sucrose phosphate synthase (SPS) activity and sucrose content in functional leaves were increased significantly, with the average increase of 30.90% and 9.48% during whole growth period, while sucrose synthase (SS) activity in storage root was decreased significantly with the average declines by 11.04%, that could promote accumulation of sucrose and fructan in storage root in treatment of HA. Amylase activity in treatment of applying HA was increased significantly with the average increase of 11.33% and 15.70%, respectively for α-amylase and β-amylase activity, that enlarged the accumulation of fructose and glucose in storage root. At harvest, the soluble sugar content was increased by 15.49% and the starch content was decreased by 3.56%. The increase of sucrose content, the decrease of the inversion quantity from soluble sugar to starch and the hydrolysis enhancement of starch in storage root are the physiological basis of increasing soluble sugar of storage root.

Key words: Sweetpotato, Humic acid, Storage root, Soluble sugar, Carbohydrate metabolic enzymes

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