Welcome to Acta Agronomica Sinica，

Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (04): 711-716.doi: 10.3724/SP.J.1006.2011.00711

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Physiological Basis of Improving Soluble Sugar Content in Sweetpotato for Table Use by Humic Acid Application

LIU Hong-Juan1,LI Zuo-Mei1,SHI Chun-Yu1,*,ZHANG Li-Ming2,*   

  1. 1 Agronomy College, Shandong Agricultural University / State Key Laboratory of Crop Biology, Tai’an 271018, China; 2 Shandong Academy of Agricultural Sciences, Jinan 250100, China
  • Received:2010-09-27 Revised:2011-01-06 Online:2011-04-12 Published:2011-02-24
  • Contact: 史春余, E-mail: scyu@sdau.edu.cn, Tel: 0538-8246259; 张立明, E-mail: zhanglm11@sina.com.cn

PDF

1370

Cited

4

Abstract: Soluble sugar content is a main index of edible quality in storage root of sweetpotato for table use.  Previous research showed that soluble sugar content in storage organs of plants was increased significantly by application of humic acid (HA), butthere have been little research on their physiological basis. The objective of this study was to clarify the physiological basis of soluble sugar content improvement in storage root of sweetpotato by using humic acid. The typical varieties of sweetpotato for table use (Beijing 553) was grown in a replicated experiment at agricultural experiment station of Shandong Agricultural University in two summer growing seasons of 2008 and 2009. The treatments included the control and treatment applied HA. The changes of starch content, soluble sugar content and relative enzymes activity during root tuber development, and sucrose content and relative enzymes activity in functional leaves were determined by using periodic sampling during storage root development. The results showed that compared with the control, sucrose phosphate synthase (SPS) activity and sucrose content in functional leaves were increased significantly, with the average increase of 30.90% and 9.48% during whole growth period, while sucrose synthase (SS) activity in storage root was decreased significantly with the average declines by 11.04%, that could promote accumulation of sucrose and fructan in storage root in treatment of HA. Amylase activity in treatment of applying HA was increased significantly with the average increase of 11.33% and 15.70%, respectively for α-amylase and β-amylase activity, that enlarged the accumulation of fructose and glucose in storage root. At harvest, the soluble sugar content was increased by 15.49% and the starch content was decreased by 3.56%. The increase of sucrose content, the decrease of the inversion quantity from soluble sugar to starch and the hydrolysis enhancement of starch in storage root are the physiological basis of increasing soluble sugar of storage root.

Key words: Sweetpotato, Humic acid, Storage root, Soluble sugar, Carbohydrate metabolic enzymes

[1]Ma D-F(马代夫), Qiu J(邱军), Fang B-P(房伯平), Sun J-Y(孙近友), Liu Y-H(刘玉恒), Liu Q-C(刘庆昌), Zhang L-M(张立明). Investigation of national sweetpotato regional test and recommendations on industrial development. Rain Fed Crops (杂粮作物), 2004, 24(5): 306–308 (in Chinese)
[2]Zhang L-M(张立明), Wang Q-M(王庆美), Wang Y-C(王荫墀). The main nutrient components and health care function of sweetpotato. Rain Fed Crops (杂粮作物), 2003, 23(3): 162–166 (in Chinese)
[3]Shi C-Y(史春余), Wang R-J(王汝娟), Liang T-B(梁太波), Wang Z-L(王振林). Characterization of carbohydrate metabolism in relation to quality of storage root in edible sweetpotato. Sci Agric Sin (中国农业科学), 2008, 41(11): 3878–3885 (in Chinese with English abstract )
[4]Zhang Q-H(张清华), Wang C-Q(王成秋), Han W-C(韩为灿), Fan R-P(范荣平), Han C-B(韩超兵). Study on the application of foliar humic acid in citrus. Humic Acid (腐植酸), 1996, (3): 13–15 (in Chinese)
[5]Ma H-G(马海刚), Xu W-L(徐万里), He S-L(何生丽), Jiang C-Y(蒋晨义), Sun H-W(孙好文), Cai Z-Y(蔡泽宇). Effect of humic acid fertilizer on applied fertilizer and quality of processing tomato. Xinjiang Agric Sci (新疆农业科学), 2009, 46(4): 772–775 (in Chinese with English abstract)
[6]Zhang J-Z(张继舟), Yuan L(袁磊), Ma X-F(马献发). Effects of humic acid on soil nutrients and salts, and on yield and quality of tomato in greenhouse. Humic Acid (腐植酸), 2008, (3): 19–22 (in Chinese)
[7]Yao H-L(姚海兰), Shi C-Y(史春余), Wang R-J(王汝娟). Effects of potassium humate on storage root quality of edible sweetpotato. Humic Acid (腐植酸), 2009, (1): 24–28 (in Chinese)
[8]Wang Y-Q(王艳群), Zhang X-G(张笑归), Xue S-C(薛世川), Gao R-T(高如泰), Zhou Y-P(周亚鹏). Effects of weathered coal and microelement fertilizer on the biologic yield and quality of crown daisy. Chin Agric Sci Bull (中国农学通报), 2008, 24(1): 293–296 (in Chinese with English abstract)
[9]Xiang G-D(项国栋), Zou D-Y(邹德乙), Li R(李荣). Study on effect of humic acid fertilizer specified for vegetable on growth of strawberry and its best application rate. Humic Acid (腐植酸), 2006, (5): 38–42 (in Chinese)
[10]Du H-Y(杜会英), Xue S-C(薛世川), Sun Z-M(孙志梅), Yang Y-M(杨云马), Wang Y-Q(王艳群), Li Y-C(李迎春). The effect of humic acid (HA) compound fertilizer on the quality and production of grape. J Agric Univ Hebei (河北农业大学学报), 2004, 27(4): 63–66 (in Chinese with English abstract)
[11]Shi C-Y(史春余), Zhang F-D(张夫道), Zhang S-Q(张树清), Li H(李辉), Fu C-G(付成高). Effects of organic-inorganic slow release fertilizers on yield and nitrogen recovery in tomato. Plant Nutr Fert Sci (植物营养与肥料学报), 2004, 37(6): 1183–1187 (in Chinese with English abstract)
[12]Padem H, Ocal A. Effects of humic acid applications on yield and some characteristics of processing tomato. Acta Hort, 2002, 487: 173–179
[13]Ertan Yildirim. Foliar and soil fertilization of humic acid affect productivity and quality of tomato. Acta Agric Scandinavica Section B-Soil Plant Sci, 2007, 57: 182–186
[14]He P(何萍), Yang J(杨金), Zhou W(周卫). The effect of humic acid (HA) compound fertilizer on yield, quality and physiological activity in tomato. Chin J Soil Sci (土壤通报), 1997, 28(6): 277–279 (in Chinese)
[15]Lin R-X(林汝湘), Xie C-S(谢春生), Feng Z-X(冯祖虾), Huang H-C(黄宏城). A study on several nutritional compositions of sweetpotato germplasm in south China. Sci Agric Sin (中国农业科学), 1995, 28(4): 39–45 (in Chinese with English abstract)
[16]Li L(李良), Liao J-X(廖嘉信), Lai Z-R(赖昭蓉). Relationship between eating quality components and physico-chemical properties in sweetpotato. J Chin Agron Assoc (中华农学会报), 1991, 156: 83–94 (in Chinese)
[17]Guo A-G(郭蔼光). Basic Biochemistry (基础生物化学). Beijing: Higher Education Press, 2008. pp 139–141, 203–205 (in Chinese)
[18]Douglas C D, Tsung M K, Frederick C F. Enzymes of sucrose and hexose metablism in development kernels of two inbreds of maize. Plant Physiol, 1988, 86: 1 013–1 019
[19]Tsai-Mei, Ou-Lee, Setter T L. Effect of increased temperature in apical regions of maize ears on starch-synthesis enzymes and accumulation of sugars and starch. Plant Physiol, 1985, 79: 852–855
[20]Yu X-J(於新建). Experimental Manual of Plant Physiology (植物生理学实验手册). Shanghai: Shanghai Scientific and Technical Publisher, 1985. pp 148–149 (in Chinese)
[21]Wardlaw I F, Willenbrink J. Carbonhydrate storage and mobilization by the culm of wheat between heading and grain maturity: the relation to sucrose synthase and sucrose-phosphate synthase. Aust J Plant Physiol, 1994, 21: 251–271
[22]Smith D A, Prescott H E. Sugar content and activity of sucrose metabolism enzymes in milled rice grain. Plant Physiol, 1989, 89: 893–896
[23]Keeling P L, Wood J R, Tyson R H, Bridges I G. Starch biosynthesis in developing wheat grain. Plant Physiol, 1988, 87: 311–319
[1] JIN Rong, JIANG Wei, LIU Ming, ZHAO Peng, ZHANG Qiang-Qiang, LI Tie-Xin, WANG Dan-Feng, FAN Wen-Jing, ZHANG Ai-Jun, TANG Zhong-Hou. Genome-wide characterization and expression analysis of Dof family genes in sweetpotato [J]. Acta Agronomica Sinica, 2022, 48(3): 608-623.
[2] ZHANG Hai-Yan, XIE Bei-Tao, JIANG Chang-Song, FENG Xiang-Yang, ZHANG Qiao, DONG Shun-Xu, WANG Bao-Qing, ZHANG Li-Ming, QIN Zhen, DUAN Wen-Xue. Screening of leaf physiological characteristics and drought-tolerant indexes of sweetpotato cultivars with drought resistance [J]. Acta Agronomica Sinica, 2022, 48(2): 518-528.
[3] ZHANG Si-Meng, NI Wen-Rong, LYU Zun-Fu, LIN Yan, LIN Li-Zhuo, ZHONG Zi-Yu, CUI Peng, LU Guo-Quan. Identification and index screening of soft rot resistance at harvest stage in sweetpotato [J]. Acta Agronomica Sinica, 2021, 47(8): 1450-1459.
[4] MA Meng, YAN Hui, GAO Run-Fei, KOU Meng, TANG Wei, WANG Xin, ZHANG Yun-Gang, LI Qiang. Construction linkage maps and identification of quantitative trait loci associated with important agronomic traits in purple-fleshed sweetpotato [J]. Acta Agronomica Sinica, 2021, 47(11): 2147-2162.
[5] Yong-Chen LIU,Cheng-Cheng SI,Hong-Juan LIU,Bin-Bin ZHANG,Chun-Yu SHI. Reason exploration for soil aeration promoting photosynthate transportation between sink and source in sweet potato [J]. Acta Agronomica Sinica, 2020, 46(3): 462-471.
[6] Shan-Bin CHEN, Si-Fan SUN, Nan NIE, Bing DU, Shao-Zhen HE, Qing-Chang LIU, Hong ZHAI. Cloning of IbCAF1 and identification on tolerance to salt and drought stress in sweetpotato [J]. Acta Agronomica Sinica, 2020, 46(12): 1862-1869.
[7] Hai-Yan ZHANG,Bei-Tao XIE,Bao-Qing WANG,Shun-Xu DONG,Wen-Xue DUAN,Li-Ming ZHANG. Evaluation of drought tolerance and screening for drought-tolerant indicators in sweetpotato cultivars [J]. Acta Agronomica Sinica, 2019, 45(3): 419-430.
[8] LI Yan-Xia,YANG Wei-Bing,YIN Yan-Ping,ZHENG Meng-Jing,CHEN Jin,YANG Dong-Qing,LUO Yong-Li,PANG Dang-Wei,LI Yong,WANG Zhen-Lin. Difference of physiological characteristics of grain weight at various kernel positions in wheat spikelets [J]. Acta Agronomica Sinica, 2019, 45(11): 1715-1724.
[9] ZHANG Hai-Yan,DUAN Wen-Xue,XIE Bei-Tao,DONG Shun-Xu,WANG Bao-Qing,SHI Chun-Yu,ZHANG Li-Ming. Effects of Drought Stress at Different Growth Stages on Endogenous Hormones and Its Relationship with Storage Root Yield in Sweetpotato [J]. Acta Agron Sin, 2018, 44(01): 126-136.
[10] DENG Chang-Zhe,YAO Hui,AN Fei-Fei,LI Kai-Mian,CHEN Song-Bi. Chromoplast Isolation and Its Proteomic Analysis from Cassava Storage Roots [J]. Acta Agron Sin, 2017, 43(09): 1290-1299.
[11] WANG Shun-Yi,LI Huan,LIU Qing,SHI Yan-Xi*. Effect of Potassium Application on Root Grow and Yield of Sweet Potato and Its Physiological Mechanism [J]. Acta Agron Sin, 2017, 43(07): 1057-1066.
[12] Lü Xiao-Kang,WEN Xiao-Xia,LIAO Yun-Cheng,LIU Yang*. Effect of ExogenousPolyamines on Mechanism of Floret Degeneration in Wheat [J]. Acta Agron Sin, 2016, 42(09): 1391-1401.
[13] SHI Xuan,WANG Ru-Yuan,TANG Jun,LI Zong-Yun,LUO Yong-Hai. Analysis of Interspecific SNPs in Sweetpotato Using a Reduced-Representation Genotyping Technology [J]. Acta Agron Sin, 2016, 42(05): 641-647.
[14] WU Chun-Hong,LIU Qing,KONG Fan-Mei,LI Huan,SHI Yan-Xi. Effects of Nitrogen Application Rates on Root Yield and Nitrogen Utilization in Different Purple Sweetpotato Varieties [J]. Acta Agron Sin, 2016, 42(01): 113-122.
[15] WANG Cui-Juan,SHI Chun-Yu,LIU Na,LIU Shuang-Rong,YU Xin-Di. Comparison of Root Characteristics and Sugar Components in Root and Leaf at Early Growth Phase of Sweet Potato Varieties with Significant Difference in Valid Storage Root Number [J]. Acta Agron Sin, 2016, 42(01): 131-140.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No. 80 Xueyuan South Rd, Beijing 100081, P. R. China E-mail: zwxb301@caas.cn
Supported by Beijing Magtech Co.Ltd