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Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (09): 1676-1682.doi: 10.3724/SP.J.1006.2011.01676

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Quantitative Determination of Peanut Seed Lipase Activity and Its Correlation with Storage Characteristics

AO Qi1,3,ZHANG Ying1,2,*,LIU Ze1,ZHANG Zheng-Zhu3,*,WU Yue-Jin2,YU Shan-Lin4,ZHANG Chen3,TENG Bin1,WU Jing-De1,JIANG Lan1   

  1. 1 Rice Research Institute, Anhui Academy of Agricultural Sciences / Anhui Province Key Laboratory of Rice Genetics and Breeding, Hefei 230031, China; 2 Anhui Province Key Laboratory of Ion Beam Bioengineering, Chinese Academy of Sciences, Hefei 230031, China; 3 Tea and Herbs Key Laboratory Safety, Anhui Agricultural University, Ministry of Agriculture, Hefei 230036, China; 4 Shandong Peanut Research Institute, Qingdao 266100, China
  • Received:2011-02-12 Revised:2011-05-27 Online:2011-09-12 Published:2011-06-28
  • Contact: 张瑛, E-mail: zhangying56888@sina.com; 张正竹, E-mail: zzz@ahau.edu.cn, Tel: 0551-2160182, 0551-2160453

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Abstract: Based on colorimetric determination method reported for rice lipase, we established a new method improving and optimizing the measurement for peanut seed lipase activity, with the optimum sampling amount of 5 mg and substrate concentration of 30 mg mL–1. The method was validated to be accurate, specific and well repeatable. The lipase activities of 216 peanut germplasm resources were detected, with significant difference (P<0.001) among them. Five with higher lipase activity and five with lower lipase activity were screened, and each two of which were selected to perform an accelerated-aging experiment under high temperature and high moisture. The results indicated that, with extension of storage time, the germination rate of peanut with lower lipase activity decreased slowly, while that of peanut with higher lipase decreased rapidly, showing a negative correlation betweenseed vigor and peanut lipase activity (correlation coefficient was –0.8875). The study provides a reference for the further research in the effect of lipase on peanut storage characteristics.

Key words: Peanut, Lipase activity, Quantitative determination, Storage characteristics

[1]Shan S-H(单世华), Wang S-B(万书波), Qiu Q-S(邱庆树), Li C-J(李春娟), Xu T-T(许婷婷), Gong Q-X(宫清轩). Quality evaluation on groundnut germplasm resources in China. Shandong Agric Sci (山东农业科学), 2007, (6): 40–42 (in Chinese with English abstract)
[2]Tang S(汤松), Yu S-L(禹山林), Liao B-S(廖伯寿), Zhang X-Y(张新友), Sun H-Y(孙海艳). Industry status, existing problems and development strategy of peanut in China. J Peanut Sci (花生学报), 2010, 39(3): 35–38 (in Chinese with English abstract)
[3]Dai L-Y(戴凌燕). Study on the effect of three pharmaceuticals on the peanut sprout germination and protein contents. Acad Period Farm Prod Proc (农产品加工?学刊), 2008, (3): 46–47 (in Chinese with English abstract)
[4]Xiao C-Z(肖昌珍), Wu Y(吴渝), Gan D-S(甘冬生), Tong C-X(童春霞), Liu G-M(刘桂梅). The analysis of biochemical quality of China peanut germplasm. Peanut Sci Technol (花生科技), 1999, (suppl): 161–166 (in Chinese)
[5]Gao H-M(高慧敏), Zhang Y(张颖). Microanalysis and fast analysis on fatty acids of peanut seed by gas chromatography. Chin Agric Sci Bull (中国农学通报), 2010, 26(13): 98–103 (in Chinese with English abstract)
[6]Li Y(李颖). Study on extension of storage period of peanut. Sci Technol Cereals, Oils Foods (粮油食品科技), 2009, 17(2): 45–47 (in Chinese with English abstract)
[7]Huang A H C. Plant lipases. In: Borstrom B, Brockman H L, eds. Lipases. Elsevier Science Publishers, Amsterdam, 1984. pp 419–442
[8]Chen X-J(陈雄金). The research of lipase and its application. Sci Technol Inform (科技信息), 2008, 19: 58 (in Chinese)
[9]Yamamoto A, Fujii Y, Yasumoto K, Mitsuda H. Partial purification and study of some properties of rice germ lipoxygenase. Agric Biol Chem, 1980, 44: 443–445
[10]Araki T, Kitaoka H. Antioxidative properties of probucol estimated by the reactivity with superoxide and by electrochemical oxidation. Chem Pharm Bull, 2001, 49: 943–947
[11]Yuan W-W(袁伟伟), Tan X-L(谭小力), Zhou J(周佳), Zhu F-G(朱福各), Xu F(胥峰), Yu L-L(于莉莉). Dynamic change of lipases activity during post-germinative growth and seed development in Brassica napus. Jiangsu J Agric Sci (江苏农业学报), 2010, 26(3): 482–486 (in Chinese with English abstract)
[12]Zhang C(张驰), Liu X-P(刘信平), Zhou D-Z(周大寨), Tian G-Z(田国政). The influence of selenium upon seeds budding and fatase of peanut. Hubei Agric Sci (湖北农业科学), 2003, 3: 36–37 (in Chinese with English abstract)
[13]Zhai Z-Q(翟征秋), Sun H-F(孙会芳), Zhang R-H(张瑞华). Effect of heavy metals Cr and Cu on seeds germination and lipase activity of peanut. J Anhui Agric Sci (安徽农业科学), 2009, 37(1): 70–71 (in Chinese with English abstract)
[14]Shen L(申琳), Zhang Z-Q(张志强), Shi L(史兰), Shi F(石凤), Fan B(范蓓), Sheng J-P(生吉萍). Changes of the activities of proteinase, Lipase, SOD and CAT during the germination of the peanut seed. J Chin Inst Food Sci Technol (中国食品学报), 2003, (suppl): 24–27 (in Chinese with English abstract)
[15]Zhang Y(张瑛), Wu Y-J(吴跃进), Yu Z-L(余增亮), Wang Y(王钰), She D-H(佘德红), Song M(宋美). The rapid detection of seeds lipase activity. China, Patent serial number: 200510037720.6. 2005. 2 (in Chinese)
[16]Liu J(刘洁), Zhang Y(张瑛), Song M(宋美), Ren C(任冲), Jiang J-Y(蒋家月), Yu Z-L(余增亮), Wu Y-J(吴跃进). Study on accurate analysis technique of rice seed lipase. Chin High Technol Lett (高技术通讯), 2007, 17(10): 1077–1081 (in Chinese with English abstract)
[17]Huang X-R(黄锡荣), Zhang W-J(张文娟), Song S-F(宋少芳), Li Y-Z(李越中), Qu Y-B(曲音波). Pectrophotometric assay of lipase activity in microemulsion. Chemistry (化学通报), 2001, (10): 659–661 (in Chinese with English abstract)
[18]Zhi J-Z(支巨振), Bi X-H(毕辛华), Du K-M(杜克敏), Chang X-L(常秀兰), Yang S-H(杨淑惠), Ren S-P(任淑萍), Wu Z-X(吴志行), Li R-F(李仁凤), Zhao J-Y(赵菊英). Rules for Agricultural Seed Testing—Germination Test (农作物种子检验规程——发芽试验). Beijing: Standard Press of China, 1995 (in Chinese)
[19]Jin J-M(金建猛), Ren L(任丽), Gu J-Z(谷建中), Fan J-L(范君龙), Liu X-Y(刘向阳). Safe storage of peanut seed cause analysis and countermeasures. Modern Agric (现代农业), 2007, (8): 41 (in Chinese)
[20]Magda A M, Tarek M M, Saleh A M, Afaf S F. Distribution of lipases in the gramineae: partial purification and characterization of esterase from Avena fatua. Bioresource Technol, 2000, 73: 227–234
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